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961.
Differential receptor subunit affinities of type I interferons govern differential signal activation
Type I interferons (IFNs) elicit antiviral, antiproliferative and immunmodulatory responses by binding to a shared cell surface receptor comprising the transmembrane proteins ifnar1 and ifnar2. Activation of differential response patterns by IFNs has been observed, suggesting that members of the family play different roles in innate immunity. The molecular basis for differential signaling has not been identified yet. Here, we have investigated the recognition of various IFNs including several human IFNalpha species, human IFNomega and human IFNbeta as well as ovine IFNtau2 by the receptor subunits in detail. Binding to the extracellular domains of ifnar1 (ifnar1-EC) and ifnar2 (ifnar2-EC) was monitored in real time by reflectance interference and total internal reflection fluorescence spectroscopy. For all IFNs investigated, competitive 1:1 interaction not only with ifnar2-EC but also with ifnar1-EC was shown. Furthermore, ternary complex formation was studied with ifnar1-EC and ifnar2-EC tethered onto solid-supported membranes. These analyses confirmed that the signaling complexes recruited by IFNs have very similar architectures. However, differences in rate and affinity constants over several orders of magnitude were observed for both the interactions with ifnar1-EC and ifnar2-EC. These data were correlated with the potencies of ISGF3 activation, antiviral and anti-proliferative activity on 2fTGH cells. The ISGF3 formation and antiviral activity correlated very well with the binding affinity towards ifnar2. In contrast, the affinity towards ifnar1 played a key role for antiproliferative activity. A striking correlation was observed for relative binding affinities towards ifnar1 and ifnar2 with the differential antiproliferative potency. This correlation was confirmed by systematically engineering IFNalpha2 mutants with very high differential antiproliferative potency. 相似文献
962.
Clark AT Smith K Muhandiram R Edmondson SP Shriver JW 《Journal of molecular biology》2007,372(4):992-1008
Sac7d and Sso7d are homologous, hyperthermophile proteins with a high density of charged surface residues and potential ion pairs. To determine the relative importance of specific amino acid side-chains in defining the stability and function of these Archaeal chromatin proteins, pK(a) values were measured for the acidic residues in both proteins using (13)C NMR chemical shifts. The stability of Sso7d enabled titrations to pH 1 under low-salt conditions. Two aspartate residues in Sso7d (D16 and D35) and a single glutamate residue (G54) showed significantly perturbed pK(a) values in low salt, indicating that the observed pH-dependence of stability was primarily due to these three residues. The pH-dependence of backbone amide NMR resonances demonstrated that perturbation of all three pK(a) values was primarily the result of side-chain to backbone amide hydrogen bonds. Few of the significantly perturbed acidic pK(a) values in Sac7d and Sso7d could be attributed to primarily ion pair or electrostatic interactions. A smaller perturbation of E48 (E47 in Sac7d) was ascribed to an ion pair interaction that may be important in defining the DNA binding surface. The small number (three) of significantly altered pK(a) values was in good agreement with a linkage analysis of the temperature, pH, and salt-dependence of folding. The linkage of the ionization of two or more side-chains to protein folding led to apparent cooperativity in the pH-dependence of folding, although each group titrated independently with a Hill coefficient near unity. These results demonstrate that the acid pH-dependence of protein stability in these hyperthermophile proteins is due to independent titration of acidic residues with pK(a) values perturbed primarily by hydrogen bonding of the side-chain to the backbone. This work demonstrates the need for caution in using structural data alone to argue the importance of ion pairs in stabilizing hyperthermophile proteins. 相似文献
963.
964.
Leila J. Hamdan Robert B. Jonas 《Journal of experimental marine biology and ecology》2007,342(2):242-252
Phytoplankton production and accumulation of extracellular organic carbon (EOC) was tracked during diel intervals in microcosms by inhibiting bacterioplankton assimilation of EOC with streptomycin and kanamycin. Bacterioplankton production (3H-thymidine incorporation) and metabolism (14C-glucose incorporation) were monitored in samples collected from the Potomac River estuary to determine the effect of the antibiotics. Particulate (i.e., raw water) primary production and EOC (i.e., water passing through 1.0 μm glass fiber filter) production rates were monitored to determine the impact of antibiotics on phytoplankton. In preliminary experiments, neither streptomycin nor kanamycin alone significantly inhibited bacterioplankton activity compared to controls, but when both were present secondary production and metabolism were reduced up to 90%, and remained as such for 45 h. During field evaluations using a streptomycin and kanamycin mixture (50 μM each) particulate primary production and EOC production were not statistically different in control and antibiotic treated samples indicating that the antibiotics did not negatively influence phytoplankton production rates. In the presence of antibiotics dissolved free amino acids (DFAA) and, to a lesser extent, monosaccharides were significantly elevated compared to controls. This study demonstrates that streptomycin and kanamycin are capable of inhibiting bacterioplankton metabolism and uptake of dissolved organic carbon (DOC) in the samples tested so that the contribution of EOC to the DOC pool and to bacterioplankton metabolism could be measured and assessed. 相似文献
965.
M.S. Koch S. Schopmeyer C.J. Madden 《Journal of experimental marine biology and ecology》2007,341(1):91-101
To examine the synergism of high temperature and sulfide on two dominant tropical seagrass species, a large-scale mesocosm experiment was conducted in which sulfide accumulation rates (SAR) were increased by adding labile carbon (glucose) to intact seagrass sediment cores across a range of temperatures. During the initial 10 d of the 38 d experiment, porewater SAR in cores increased 2- to 3-fold from 44 and 136 μmol L− 1 d− 1 at 28-29 °C to 80 and 308 μmol L− 1 d− 1 at 34-35 °C in Halodule wrightii and Thalassia testudinum cores, respectively. Labile C additions to the sediment resulted in SAR of 443 and 601 μmol L− 1 d− 1 at 28-29 °C and 758 to 1,557 μmol L− 1 d− 1 at 34-35 °C in H. wrightii and T. testudinum cores, respectively. Both T. testudinum and H. wrightii were highly thermal tolerant, demonstrating their tropical affinities and potential to adapt to high temperatures. While plants survived the 38 d temperature treatments, there was a clear thermal threshold above 33 °C where T. testudinum growth declined and leaf quantum efficiencies (Fv/Fm) fell below 0.7. At this threshold temperature, H. wrightii maintained shoot densities and leaf quantum efficiencies. Although H. wrightii showed a greater tolerance to high temperature, T. testudinum had a greater capacity to sustain biomass and short shoots under thermal stress with labile C enrichment, regardless of the fact that sulfide levels in the T. testudinum cores were 2 times higher than in the H. wrightii cores. Tropical seagrass tolerance to elevated temperatures, predicted in the future with global warming, should be considered in the context of the sediment-plant complex which incorporates the synergism of plant physiological responses and shifts in sulfur biogeochemistry leading to increased plant exposure to sulfides, a known toxin. 相似文献
966.
To assess the colonization success of pumpkinseed Lepomis gibbosus on the Iberian Peninsula, life-history attributes of pumpkinseed populations from Spanish water bodies were compared to populations in the northern and southern parts of its native range, as well as to those of English water bodies where introduced populations have shown minimal natural range expansion. Discriminate function analysis using five population characteristics [mean age at maturity, mean total length ( L T ) at maturity, gonado-somatic index ( I G ), mean L T at age 2 years as an indication of juvenile growth rate and relative body condition] strongly differentiated populations from the four regional study areas. Spanish populations were early maturing, showed moderate juvenile growth rate and L T at maturity and high I G relative to the other population groups. Spanish populations matured significantly earlier than Canadian and English populations, but not southern U.S.A. populations. Spanish populations, however, had a significantly higher I G than southern U.S.A. populations, suggesting greater reproductive output. Considering these differences in the context of the Winemiller and Rose triangular life-history strategy model, the pumpkinseed exhibits 'equilibrium' life-history traits that have made other non-native fishes successful invaders in areas where habitat alteration has resulted in more predictable discharge regimes and water levels. Populations in Iberia, however, appear to have achieved their success by adopting a more 'opportunistic' life-history strategy than their native counterparts. High levels of life-history plasticity appear to contribute to the success of this species on the Iberian Peninsula. 相似文献
967.
Water use efficiency in C3 cereals under Mediterranean conditions: a review of physiological aspects
In this review, we will discuss physiological traits of C3 cereals related to water use efficiency (WUE) in Mediterranean environments, from leaf (WUEinstantaneous) to crop level (WUEyield or ‘water productivity’). First, we analyse the WUEinstantaneous and the possible trade‐off between improving this parameter and growth/yield performance. Ways to ameliorate WUE without penalties are discussed. We also analyse in what cases breeding by high or low WUEinstantaneous is a suitable criterion to maintain grain yield under drought (Mediterranean) conditions. This question is approached in the framework of carbon isotope discrimination, (Δ13C), the main indirect parameter used to integrate (at time and space scale) the WUEinstantaneous in C3 plants. A negative correlation between these two parameters has been confirmed by several studies. The relationship between Δ13C and grain yield, however, is more complex, and may differ from one environment to another. In Mediterranean conditions with moderate or no water stress, a positive correlation between Δ13C and grain yield is found in barley and wheat, whereas in ‘stored‐water’ crops (such as in some regions of Australia), lower Δ13C (i.e. higher WUEinstantaneous) is associated with higher grain yield, particularly in more stressful conditions. These apparent inconsistencies and their possible implications for plant breeding are discussed. One physiological trait that has received minor attention in attempts to improve WUEinstantaneous is the role of ear photosynthesis. Ears of barley and durum wheat have a higher WUEinstantaneous than the flag leaf, both in well‐watered and in drought conditions. The underlying causes of the higher WUEinstantaneous of ears are not fully understood, but their refixation capacity (i.e. the capacity to re‐assimilate respired carbon dioxide) could be important. Although the genotypic variability of this trait has not been extensively studied, some data support the idea that variation in refixation capacity may be attributable to genetic factors. At the crop level, decreasing soil evaporation is a crucial factor in efforts to improve the WUEyield in Mediterranean conditions, and fast initial growth of the crop (i.e. early vigour) seems to be relevant. In wheat, modern varieties with dwarfing genes (giberellic acid – insensitive) have higher yields but, concomitantly, they have lower initial growth performance. Recently, semi‐dwarf cultivars (giberellic acid – sensitive) with high grain yield and simultaneously high early vigour were found, opening new avenues to increase WUEyield in wheat. The negative effects of futile water loss by cuticular and nocturnal transpiration are also commented. Finally, we discuss some agronomic practices (in particular, ‘deficit irrigation’ systems) linked to physiological traits that confer higher WUEyield,, in particular, in the cases of Mediterranean regions. 相似文献
968.
969.
We investigated the responses of photon-saturated photosynthesis rate (P
sat) and its simultaneous acclimation of anatomy and nitrogen use patterns of current needles of Korean pine (Pinus koraiensis) seedlings grown under factorial combinations of two nitrogen levels and irradiances. Although N supply resulted in a significant
increase of N content in needles under both irradiances, the increase of P
sat tended to be suppressed only in shade (S). The significant increase of P
sat in full sunlight (O) was associated with the increase of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) and chlorophyll
(Chl) contents. In contrast, small increase of Chl content and no increase of RuBPCO content were found in S (90 % cut of
full irradiance), which would result in a small increase of P
sat. This result suggests that extra N is stocked in needles under shade for the growth in next season. With N supply, a significant
decrease of specific leaf area (SLA) was detected only in O. This decrease of SLA was due to the increase of density of needle.
Furthermore, the increase of needle density was not due to the increased number and size of mesophyll cells, but the increased
density of each mesophyll cell. Therefore, although SLA changed in O, the change did not involve anatomical adaptation to
use increased N effectively, at least observable by light microscopy. Hence, even though the SLA would change, N deposition
will improve the photosynthetic capacity of Korean pine seedlings, not through the development of needle anatomy but through
improvement of the allocation of N in both irradiances. 相似文献
970.
Gas exchange, chlorophyll (Chl) fluorescence, and contents of some metabolites in two genotypes of jasmine (Jasminum sambac), single petal (SP) and double petal (DP) one, were analyzed during dehydration and re-hydration. Water stress significantly
decreased net photosynthetic rate, stomatal conductance, and maximum photochemical efficiency (Fv/Fm) in both jasmine genotypes, but increased minimum fluorescence (F0) only in DP-jasmine. Water stress also decreased starch content, while increased contents of total soluble sugars and proline
in leaves of both genotypes. SP-jasmine demonstrated higher drought tolerance as evidenced by maintaining higher gas exchange
and photochemical efficiency and lower alteration of metabolites than DP-jasmine. Recovery analysis revealed that drought-induced
injury in photosynthetic machinery in jasmine plants was reversible. DP-jasmine exhibited a slow recovery of drought-induced
impairment in photosynthetic activity and associated metabolites, suggesting that this genotype had lower capacity to adapt
to water limited condition. Higher yield stability of SP-than that of DP-jasmine under rain-fed condition finally confirmed
higher drought tolerance of SP-jasmine. 相似文献