全文获取类型
收费全文 | 6115篇 |
免费 | 395篇 |
国内免费 | 447篇 |
出版年
2024年 | 11篇 |
2023年 | 109篇 |
2022年 | 96篇 |
2021年 | 160篇 |
2020年 | 189篇 |
2019年 | 232篇 |
2018年 | 166篇 |
2017年 | 178篇 |
2016年 | 199篇 |
2015年 | 246篇 |
2014年 | 260篇 |
2013年 | 367篇 |
2012年 | 211篇 |
2011年 | 250篇 |
2010年 | 203篇 |
2009年 | 325篇 |
2008年 | 355篇 |
2007年 | 346篇 |
2006年 | 330篇 |
2005年 | 268篇 |
2004年 | 246篇 |
2003年 | 268篇 |
2002年 | 183篇 |
2001年 | 158篇 |
2000年 | 161篇 |
1999年 | 120篇 |
1998年 | 134篇 |
1997年 | 137篇 |
1996年 | 98篇 |
1995年 | 88篇 |
1994年 | 97篇 |
1993年 | 93篇 |
1992年 | 105篇 |
1991年 | 70篇 |
1990年 | 71篇 |
1989年 | 60篇 |
1988年 | 53篇 |
1987年 | 45篇 |
1986年 | 53篇 |
1985年 | 60篇 |
1984年 | 28篇 |
1983年 | 21篇 |
1982年 | 27篇 |
1981年 | 29篇 |
1980年 | 14篇 |
1979年 | 10篇 |
1978年 | 7篇 |
1977年 | 6篇 |
1973年 | 3篇 |
1972年 | 5篇 |
排序方式: 共有6957条查询结果,搜索用时 31 毫秒
891.
J G Lewis R P Learmonth P V Attfield K Watson 《Journal of industrial microbiology & biotechnology》1997,18(1):30-36
Fourteen wild-type baking strains of Saccharomyces cerevisiae were grown in batch culture to true stationary phase (exogenous carbon source exhausted) and tested for their trehalose
content and their tolerance to heat (52°C for 4.5 min), ethanol (20% v/v for 30 min), H2O2 (0.3 M for 60 min), rapid freezing (−196°C for 20 min, cooling rate 200°C min−1), slow freezing (−20°C for 24 h, cooling rate 3°C min−1), salt (growth in 1.5 M NaCl agar) or acetic acid (growth in 0.4% w/v acetic acid agar) stresses. Stress tolerance among
the strains was highly variable and up to 1000-fold differences existed between strains for some types of stress. Compared
with previously published reports, all strains were tolerant to H2O2 stress. Correlation analysis of stress tolerance results demonstrated relationships between tolerance to H2O2 and tolerance to all stresses except ethanol. This may imply that oxidative processes are associated with a wide variety
of cellular stresses and also indicate that the general robustness associated with industrial yeast may be a result of their
oxidative stress tolerance. In addition, H2O2 tolerance might be a suitable marker for the general assessment of stress tolerance in yeast strains. Trehalose content
failed to correlate with tolerance to any stress except acetic acid. This may indicate that the contribution of trehalose
to tolerance to other stresses is either small or inconsistent and that trehalose may not be used as a general predictor
of stress tolerance in true stationary phase yeast.
Received 10 October 1995/ Accepted in revised form 10 September 1996 相似文献
892.
Seed germination and seedling establishment patterns have been used to classify species as shade tolerant or intolerant. The main objective of this research was to investigate, under controlled conditions, seed germination of species from different successional positions as well as to follow seed germination and seedling survival under natural shade in the field. The species studied were Solarium granuloso‐leprosum, Trema micrantha, Cecropia pachystachya, Croton piptocalyx, Bauhinia forficata subsp. pruinosa. Senna macranthera, Schizolobium parahyba, Piptadenia gonoacantha, Chorisia speciosa, Pseudobombax grandiflorum, Ficus guaranitica, Esenbeckia leiocarpa, Pachystroma longifolium, Myroxylon peruiferum, and Hymenaea courbaril. Field trials were carried out at Santa Genebra Municipal Reserve, Campinas, SP, Brazil, at the forest edge and in the understory. No significant correlations were detected between successional status and seed size or seed water content. Light‐regulated germination was present only in small‐seeded species. In field experiments, most species, including the light‐sensitive ones, were able to germinate under the canopy, where a low red/far‐red ratio predominates. Most species, mainly those of early‐ and intermediate successional positions, presented low seedling survival rates under shade. Myroxylon peruiferum was the most shade tolerant species, while 5. granuloso‐leprosum, C. speciosa, P. gonoacantha, F. guaranitica, T. micrantha, and 5. parahyba were the most shade intolerant. These latter species showed little or no survival under the shade conditions. 相似文献
893.
C. Nielsen G. Holdensgaard H. C. Petersen B. Th. Björnsson S. S. Madsen 《Journal of fish biology》2001,59(1):28-44
Out of five strains of Atlantic salmon Salmo salar of 1+ years released upstream of a fyke net in the River Gudenaa in 1996, three, Lagan, Ätran and Corrib, migrated immediately, 50% of the recaptured fish reaching the net in 3–6 days. Burrishoole and Conon fish migrated with a 15–19 day delay. Smolt development in 1997 at the hatchery showed a spring surge in gill Na+ , K+ -ATPase activity in all strains which was correlated with increased seawater tolerance. Differences in the timing of gill enzyme development matched the observed migration pattern well. Lagan, Ätran and Corrib strains reached high enzyme activity earlier than the Burrishoole and Conon strains, and strains with delayed enzyme development and migration showed a delayed regression of seawater tolerance compared with the early strains. Inter-strain differences in plasma growth hormone profiles could not be related to the observed patterns of Na+ , K+ -ATPase and seawater tolerance development. The study gives evidence of genetic influence on the timing and intensity of smolting and subsequent migration in Atlantic salmon. 相似文献
894.
The salinity tolerance of 62 strains of Pfiesteria and Pfiesteria‐like heterotrophic dinoflagellates was measured. All strains were acclimated at 12 psu for at least 1 year before experimentation. Strains isolated from the Chesapeake Bay and Neuse River systems tolerated lower salinities than strains isolated from the Wilmington River system (P< 0.005). Swimming cells were still observed after 5 days at 0.5 psu for one strain, and at 1 psu for most other Chesapeake Bay and Neuse River strains. Swimming cells for the Wilmington River were still observed after 5 days at 3–5 psu, but no swimming cells were observed at ≤ 2 psu. With regard to the upper salinity tolerance, the Wilmington River strains tolerated higher salinities than the Chesapeake Bay and Neuse River systems (P< 0.005). Most Wilmington River strains were swimming after 5 days at salinities ≥ 50 psu, whereas the Chesapeake Bay and Neuse River system strains rarely had swimming cells at salinities exceeding 35–45 psu. For all three water systems and for both lower and higher salinities, cells apparently encysted in many instances. However, when salinities were returned to 12 psu, swimming cells often re‐appeared. Statistically significant geographic differences in salinity tolerance suggest a geographic adaptation has occurred and that salinity tolerance is under genetic control. The results also suggest there is diversity among the strains. 相似文献
895.
Allele frequencies were determined at 14 microsatellite loci in 284 female and 50 male rainbow trout that were sampled throughout the spawning season from a commercial trout farm. Phenotypic selection has expanded the spawning season of the broodstock from 2 weeks to 8 months. Females maturing in different seasons showed significantly different allelic distributions (P<0·001) at all loci. The spawning time for the majority of females sampled could be predicted based on their genotypic information [chromosome segment sharing coefficient (CSSC) values]. CSSC analyses assigned 100, 56, 76 and 68% of summer, fall, winter, and spring spawning females, respectively to the season from which their gametes were actually collected. Alternatively, only 38 and 14% of summer and spring spawning XY males respectively, were assigned to the correct season. Loci linked to thermal tolerance and spawning time quantitative trait loci (QTL) showed significantly greater heterogeneity (higher average Ds values) in allele frequencies than those not known to be linked to QTL based on previous work. Thus, phenotypic selection for spawning time has led to concomitant changes in allele frequencies at markers of QTL. This suggests that the QTL detected in our previous work have detectable effects in fish from other genetic backgrounds. 相似文献
896.
897.
898.
In this paper, we continue our analysis of Na+ and K+ uptake by mid-vegetative Spergularia marina (L.) Griseb. plants growing on 0.2x sea water medium, with attention to the relationship of ion uptake and growth. In the first part of the paper, growth analysis techniques are used to compare relative growth rates (RGR) and relative accumulation rates (RAR) for Na+ and K+. Under constant growth conditions, a high correlation between RGR and RAR indicated that growth and accumulation of both ions were well balanced, resulting in Na+ and K+ concentrations within the plants which were stable after adjustement to the saline medium. The analysis confirmed the existence of a Na+ -related growth stimulation in S. marina and an associated increase in the efficiency of K+ utilization for growth. When plants were subjected to more rapid salinization and step changes in the light intensity of the growth chamber, RGR and RAR were again similar, even through the discontinuities in growth conditions, suggesting that growth and ion accumulation were co-regulated rather than simply correlated. The growth analysis data were then transformed to give net uptake rates for Na+ and K+ and the results were compared to those of isotope studies under similar growth conditions. In roots, the rates estimated by the two techniques differed substantially; net uptake rates reflected primarily growth, while isotope studies indicated a substantial ion exchange rate between mature cells and the growth medium. The rates of transport of either Na+ or K+ to the shoot were very similar using the two estimation techniques. As the rates measured with isotopes were taken from studies lasting at most a few hours, this suggested a very rapid turnover of the upwardly mobile Na+ and K+ pools in the roots. 相似文献
899.
Sayed H. Raza Habib R. Athar Muhammad Ashraf Amjad Hameed 《Environmental and Experimental Botany》2007,60(3):368-376
Modulation of water relations, activities of antioxidant enzymes and ion accumulation was assessed in the plants of two wheat cultivars S-24 (salt tolerant) and MH-97 (moderately salt sensitive) subjected to saline conditions and glycinebetaine (GB) applied foliarly. Different levels of GB, i.e., 0 (unsprayed), 50 and 100 mM (in 0.10% Tween-20 solution) were applied to the wheat plants at the vegetative growth stage. Leaf water potential, leaf osmotic potential and turgor potential were decreased due to salt stress. Salt stress increased the Na+ and Cl− accumulation coupled with a decrease in K+ and Ca2+ in the leaves and roots of both cultivars thereby decreasing tissue K+/Na+ and Ca2+/Na+ ratios. Furthermore, salt stress decreased the activities of superoxide dismutase (SOD), whereas it increased the activities of catalase (CAT) and peroxidase (POD) in both wheat cultivars. However, accumulation of GB in the leaves of both wheat cultivars was consistently increased with an increase in concentration of exogenous GB application under both non-saline and saline conditions. Accumulation of Na+ was decreased with an increase in K+ accumulation upon a consistent increase in GB accumulation under salt stress conditions thereby resulting in better K+/Na+ and Ca2+/Na+ ratios in the leaves and roots. High accumulation of GB and K+ mainly contributed to osmotic adjustment, which is one of the factors known to be responsible for improving growth and yield under salt stress. The activities of all antioxidant enzymes, SOD, CAT and POD were enhanced by GB application in cv. MH-97 under saline conditions, whereas all these except SOD were reduced in cv. S-24. It is likely that both applied GB and intrinsic SOD scavenged ROS in the tolerant cultivar thereby resulting into low activities of CAT and POD enzymes under salt stress. In conclusion, the adverse effects of salt stress on wheat can be alleviated by the exogenous application of 100 mM GB by modulating activities of antioxidant enzymes and changes in water relations and ion homeostasis. Furthermore, effectiveness of GB application on regulation of activities of antioxidant enzymes was found to be cultivar-specific. 相似文献
900.
1. Sedimentary akinetes (resting stages) may represent significant potential inocula for nuisance blooms of cyanobacteria. We studied the effects of salinity and sediment source on the germination and subsequent growth of Anabaena flos‐aquae akinetes from a shallow, tidally influenced lake. 2. Surface sediments collected from littoral and open‐water sites were used as inocula to culture A. flos‐aquae akinetes in four salinities (0.1, 2.2, 4.4 and 6.5) over 22 days. Akinete germination and development was followed by counting developmental stages every second day. 3. Filament growth, but not akinete germination, was inhibited by salinity and there were significantly fewer filaments at 6.5 than at 0.1 and 2.2. Cultures inoculated with littoral sediment had more akinetes, germlings and filaments than those inoculated with open‐water sediment. 4. Sediment is a potential source of inocula for Anabaena blooms in the lake, which potentially could develop solely from this source because germination and subsequent filament growth do not depend on the existence of an initial pelagic Anabaena population. 相似文献