全文获取类型
收费全文 | 11359篇 |
免费 | 867篇 |
国内免费 | 1604篇 |
专业分类
13830篇 |
出版年
2024年 | 61篇 |
2023年 | 251篇 |
2022年 | 297篇 |
2021年 | 377篇 |
2020年 | 405篇 |
2019年 | 488篇 |
2018年 | 461篇 |
2017年 | 402篇 |
2016年 | 334篇 |
2015年 | 368篇 |
2014年 | 649篇 |
2013年 | 827篇 |
2012年 | 464篇 |
2011年 | 591篇 |
2010年 | 501篇 |
2009年 | 542篇 |
2008年 | 574篇 |
2007年 | 676篇 |
2006年 | 548篇 |
2005年 | 465篇 |
2004年 | 339篇 |
2003年 | 343篇 |
2002年 | 340篇 |
2001年 | 277篇 |
2000年 | 253篇 |
1999年 | 190篇 |
1998年 | 156篇 |
1997年 | 147篇 |
1996年 | 131篇 |
1995年 | 126篇 |
1994年 | 135篇 |
1993年 | 86篇 |
1992年 | 109篇 |
1991年 | 130篇 |
1990年 | 72篇 |
1989年 | 74篇 |
1988年 | 52篇 |
1987年 | 57篇 |
1985年 | 111篇 |
1984年 | 170篇 |
1983年 | 137篇 |
1982年 | 169篇 |
1981年 | 119篇 |
1980年 | 150篇 |
1979年 | 138篇 |
1978年 | 109篇 |
1977年 | 119篇 |
1976年 | 74篇 |
1975年 | 54篇 |
1974年 | 54篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
101.
Godfrey C. Akani Luca Luiselli Edem A. Eniang Charity C. Amuzie Nwabueze Ebere 《African Journal of Ecology》2008,46(4):533-539
The ecology of the blindsnake, Typhlops punctatus punctatus in five selected study plots in the River Niger Delta (Port Harcourt, Nigeria) was investigated. A total of 207 specimens were collected, 43% occurring under stones, 30% under leaf litter and 15% under logs. The number of snakes found under cover objects was significantly influenced by the number of cover objects lifted up. GLM analysis revealed that there was no difference in terms of number of snakes observed, once the relative effect of the different number of cover objects lifted up was taken into account, either among study plots or among types of cover objects. These snakes appeared to have a strong affinity for loamy soils, with moisture content of 8–18%, high organic matter (1–6%) and slightly acid pH of 5.4–6.9. Stomach content analysis revealed that these snakes fed mainly upon ants and termites. A case of predation by the toad Bufo maculatus on a juvenile blindsnake was also reported. 相似文献
102.
Rutkowska J Koskela E Mappes T Speakman JR 《Proceedings. Biological sciences / The Royal Society》2011,278(1720):2962-2969
Sex-allocation theories generally assume differential fitness costs of raising sons and daughters. Yet, experimental confirmation of such costs is scarce and potential mechanisms are rarely addressed. While the most universal measure of physiological costs is energy expenditure, only one study has related the maternal energy budget to experimentally controlled offspring sex. Here, we experimentally test this in the bank vole (Myodes glareolus) by simultaneously manipulating the litter's size and sex ratio immediately after birth. Two weeks after manipulation, when mothers were at the peak of lactation and were pregnant with concurrent litters, we assessed their energy budget. We found that maternal food consumption and daily energy expenditure increased with the size of the litters being lactated. Importantly, the effects of offspring sex on energy budget depended on the characteristics of the simultaneously gestating litters. Specifically, the mothers nursing all-male litters and concurrently pregnant with male-biased litters had the highest energy expenditure. These had consequences for the next generation, as size of female offspring from the concurrent pregnancy of these mothers was compromised. Our study attests a higher cost of sons, consequently leading to a lower investment in them, and reveals the significance of offspring sex in moulding the trade-off between current and future maternal investment. 相似文献
103.
Following is the first publication of a previously unknown translation by L.S. Vygotsky from Hebrew of a fictional short story by M.J. Berdyczewski, a Jewish writer popular at the beginning of the twentieth century. The commentary by the authors of this article reconstructs the context of Vygotsky’s life situation during the prerevolutionary period. Particular attention is focused on the questions of ethnic and cultural self-determination that were confronting him. The cultural contexts essential for an understanding and interpretation of the artistic and world-view features of the text of the short story are explored. A comparison is drawn to show the connection between the themes addressed in the story and the substantive problems that Vygotsky would subsequently deal with as a professional psychology: the artistic inner experience, moral choice, and cultural mediation. 相似文献
104.
天然产物中透明质酸酶抑制剂的研究 总被引:4,自引:0,他引:4
透明质酸酶抑制剂是对透明质酸酶的激活有抑制作用的物质。透明质酸酶是透明质酸的特异性裂解酶,而透明质酸在人体许多发育和调控过程中起重要作用,抑制透明质酸酶的活性可使透明质酸不被分解,维持正常的生理功能,笔者对天然产物中透明质酸酶抑制的研究开发进行了概述,并探讨了透明质酸酶抑制在医药及保健食品工业中的应用潜力。 相似文献
105.
水稻是世界上最早驯化的重要粮食作物之一。水稻芒可以保护水稻种子不被鸟琢食,是水稻重要的驯化性状之一。芒在野生稻中普遍存在,对野生稻的生存和传播至关重要,然而在驯化和人工选择过程中该性状逐渐被淘汰。定位和克隆水稻长芒相关基因是研究水稻芒驯化遗传机制的基础。本研究以籼稻恢复系东南恢810为受体、漳浦野生稻为供体构建的146个染色体片段置换系(chromosome segment substitution lines, CSSLs)为研究材料,调查了146个CSSLs株系和双亲的芒长,结果表明在4个置换系中检测到1个控制水稻芒长主效基因GAD1-2,位于水稻第8号染色体;利用重叠代换作图法,将GAD1-2定位在Ind8-10和RM4936标记之间,遗传距离约为4.75 Mb。选择分离群体中的显性单株,利用开发的标记,最终将GAD1-2 基因定位在两个 Indel 标记之间,两者间的物理距离约为27 kb,该区域内只有两个候选基因Os08g0485500和Os08g0485400。经测序和分析表明,Os08g0485500是GAD1-2的候选基因,GAD1-2在保守的ORF区域存在6个碱基缺失,导致丝氨酸和半胱氨酸这两个氨基酸缺失,从而表现长芒的性状;在Os08g0485500基因位点已克隆了1个控制水稻芒长的GAD1基因,推测GAD1-2与GAD1为等位基因。本研究为进一步理解水稻起源演化和水稻芒长发育基因的遗传机制奠定了基础。 相似文献
106.
Presence of isolated beta or betagamma domains of streptokinase (SK) increased the catalytic activity of staphylokinase (SAK)-plasmin (Pm) complex up to 60%. In contrast, fusion of SK beta or betagamma domains with the C-terminal end of SAK drastically reduced the catalytic activity of the activator complex. The enhancement effect mediated by beta or betagamma domain on Pg activator activity of SAK-Pm complex was reduced greatly (45%) in the presence of isolated kringles of Pg, whereas, kringles did not change cofactor activity of SAK fusion proteins (carrying beta or betagamma domains) significantly. When catalytic activity of SAK-microPm (catalytic domain of Pm lacking kringle domains) complex was examined in the presence of isolated beta and betagamma domains, no enhancement effect on Pg activation was observed, whereas, enzyme complex formed between microplasmin and SAK fusion proteins (SAKbeta and SAKbetagamma) displayed 50-70% reduction in their catalytic activity. The present study, thus, suggests that the exogenously present beta and betagamma interact with Pg/Pm via kringle domains and elevate catalytic activity of SAK-Pm activator complex resulting in enhanced substrate Pg activation. Fusion of beta or betagamma domains with SAK might alter these intermolecular interactions resulting in attenuated functional activity of SAK. 相似文献
107.
Abstract
Broad scale survey distributions of fish are dominated by some extremely high catches. With a novel survey design we resolved
the small-scale fish distribution in the spatio-temporal vicinity of these extreme hauls and showed that in the North Sea
they generally do not occur in isolation. An additional case study where stomach contents of fish predators were analyzed
revealed that they actually indicate aggregations of piscivorous fish predators on prey aggregations. We show that the predation
impact can reach immense dimensions, an aggregation of more than 50 million juvenile cod (Gadus morhua) was entirely wiped out in 5 days by predatory whiting (Merlangius merlangus), aggregating on these juveniles in an area of approximately 18 km2. The consumption of only 32 hot spots of similar magnitude as observed in our study adds up to the average size of an incoming
North Sea cod year class. These findings support the hypothesis of predation as the major source of mortality in young-of-the-year
demersal fish species and questions the generality of fish aggregation as an effective anti-predator strategy. This study
highlights the system-wide structuring force of small-scale predation hot spots and further points to the importance of a
more realistic implementation of local high-intensity predation events in food web models.
Electronic supplementary material: The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
108.
The long-lived, light-induced radical YD• of the Tyr161 residue in the D2 protein of Photosystem II (PSII) is known to magnetically interact with the CaMn4 cluster, situated ∼ 30 Å away. In this study we report a transient step-change increase in YD• EPR intensity upon the application of a single laser flash to S1 state-synchronised PSII-enriched membranes from spinach. This transient effect was observed at room temperature and high applied microwave power (100 mW) in samples containing PpBQ, as well as those containing DCMU. The subsequent decay lifetimes were found to differ depending on the additive used. We propose that this flash-induced signal increase was caused by enhanced spin relaxation of YD• by the OEC in the S2 state, as a consequence of the single laser flash turnover. The post-flash decay reflected S2 → S1 back-turnover, as confirmed by their correlations with independent measurements of S2 multiline EPR signal and flash-induced variable fluorescence decay kinetics under corresponding experimental conditions. This flash-induced effect opens up the possibility to study the kinetic behaviour of S-state transitions at room temperature using YD• as a probe. 相似文献
109.
Primates tend to prefer specific plant foods, and primate home ranges may contain only a subset of food species present in an area. Thus, primate feeding strategies should be sensitive to the phenology of specific species encountered within the home range in addition to responding to larger scale phenomena such as seasonal changes in rainfall or temperature. We studied three groups of Javan gibbons (Hylobates moloch) in the Gunung Halimun‐Salak National Park, Indonesia from April 2008 to March 2009 and used general linear mixed models (GLMM) and a model selection procedure to investigate the effects of variation in fruit and flower availability on gibbon behavior. Preferred foods were defined as foods that are overselected relative to their abundance, while important food species were those that comprised >5% of feeding time. All important species were also preferred. Season and measurements of flower and fruit availability affected fruit‐feeding time, daily path lengths (DPL), and dietary breadth. Models that included the availability of preferred foods as independent variables generally showed better explanatory power than models that used overall fruit or flower availability. For one group, fruit and preferred fruit abundance had the strongest effects on diets and DPL in the models selected, while another group was more responsive to changes in flower availability. Temporal variation in plant part consumption was not correlated in neighboring groups. Our results suggest that fine‐scale local factors are important determinants of gibbon foraging strategies. Am. J. Primatol. 74:1154‐1167, 2012. © 2012 Wiley Periodicals, Inc. 相似文献
110.
JAMES R. CAREY NIKOS T. PAPADOPOULOS STELLA PAPANASTASIOU ALEXANDROS DIAMANTIDIS CHRISTOS T. NAKAS 《Ecological Entomology》2012,37(5):359-369
1. A simple, low‐cost approach to estimating population ageing was introduced based on a modified version of the captive cohort method – a technique developed earlier (Carey et al., Aging Cell, 7 , 426–437, 2008) in which information on the remaining lifespans of live‐captured medflies of unknown age is used to estimate the overall population age structure. 2. To test this approach approximately 1200 medflies near Volos, Greece were live captured from daily sampling over a 3‐month field season. 3. This simplified method reported: (i) an extraordinary post‐capture longevity of wild medflies in early season (>200 days in longest lived); (ii) a decrease of 50–75 days in the mean longevity from early‐season to late‐season flies; (iii) seasonality of frailty as indicated by the shorter‐lived flies in late autumn; (iv) cessation of fly emergence in late season as indicated by the absence of long‐lived individuals (indicating newly emerged at capture) sampled in the autumn; and (v) increase in mean age from about 20 days in early season to approximately 60 days in late season. 4. The applications of this simplified captive cohort method are discussed, including its use in the analysis of insect vector populations, Drosophila ecology and ageing in the wild, demographic toxicology, and age bias in sampling. 相似文献