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171.
172.
Reduction of a cytochrome b following excitation by a single, short, near-saturating light flash has been demonstrated in Chromatium vinosum chromatophores. The extent of reduction is increased by addition of antimycin. The cytochrome has an α-band maximum at 562 nm in the presence of antimycin.The cytochrome b reduction is most readily observed in the presence of antimycin at high redox potential when cytochrome c-555 is oxidised before excitation. Under these conditions the half-time for reduction is about 20 ms, and the extent is about 0.5 mol of cytochrome b reduced per mol of reaction center oxidised. This extent of reduction is observed on the first flash-excitation from the dark-adapted state, and there was no indication that the reaction center quinone acceptor complex acted as a two-electron accumulating system. With cytochrome c-555 reduced before excitation, the extent of cytochrome b reduction is approximately halved. The factors which result in substoichiometric cytochrome b reduction are not yet understood.Agents which appear to inhibit primary acceptor oxidation by the secondary acceptor (UHDBT, PHDBT, DDAQQ, HOQNO, o-phenanthroline), inhibit reduction of the cytochrome b. DBMIB inhibits cytochrome b reduction but does not appear to inhibit primary acceptor oxidation.These observations confirm that a cytochrome b receives electrons delivered from the primary acceptor complex, and indicate that the photoreduced cytochrome b is reoxidised via an antimycin-sensitive pathway. 相似文献
173.
Marilynn E. Etzler Carl Borrebaeck 《Biochemical and biophysical research communications》1980,96(1):92-97
A glycoprotein from the stems and leaves of the plant that cross reacts with antibodies to the seed lectin has been found to bind to affinity columns of blood group A + H substance covalently linked to Sepharose. This binding of the cross reactive material to the affinity resin differs from that of the seed lectin in that it is easily dissociated with 0.15 M NaCl. Affinity electrophoresis using entrapped blood group A + H substance shows that the carbohydrate binding activity of the cross reactive material is weakly inhibited with N-acetyl-D-galactosamine and N-acetyl-D-glucosamine. Glucose, mannose and galactose gave no inhibition when tested at concentrations of 50 mM. These data indicate that the specificity of the cross reactive material is somewhat different from the N-acetyl-D-galactosamine specificity of the seed lectin. The significance of these findings is discussed in relation to the structural similarities of the cross reactive material and the seed lectin. 相似文献
174.
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176.
J H Miller M P Calos D Galas M Hofer D E Büchel B Müller-Hill 《Journal of molecular biology》1980,144(1):1-18
The lac region of Escherichia coli, carried on an F′ lacproB episome, was used as a target for the transposition of several transposable elements. Tn9 shows a preferential integration (by a factor of 50) into a region extending from the end of the Z gene through the Y gene. Throughout the remainder of the lacI, Z and Y genes one other short region, located in the middle of the I gene, is favored for integration. Within these favored regions many different integration points are evident. Inspection of the DNA sequence for the I and Y genes, and parts of the Z gene, shows a strong correlation between A + T richness and regions of preferential integration. Tn5 insertions follow a similar pattern, although with less preference; whereas Tn10 insertions (provided by T. J. Foster), also favor the Y gene and the end of Z, but are distributed among fewer integration points. Most of the Tn3 insertions into the episome are accompanied by a nearby or adjacent deletion. 相似文献
177.
C T Gombar W P Tong D B Ludlum 《Biochemical and biophysical research communications》1979,90(3):878-882
A crosslinked dinucleoside, 1,2-(diguanosin-7-yl) ethane, has been isolated from the reaction of guanosine with the antitumor agent, BCNU. The formation of this product suggests that DNA crosslinking, which may be responsible for the cytotoxicity of BCNU, could occur through such dinucleosides. 相似文献
178.
Isolation and sequence determination of a peptide located in or near the active site of bovine muscle pyruvate kinase 总被引:1,自引:0,他引:1
S C Johnson T Bailey R R Becker J M Cardenas 《Biochemical and biophysical research communications》1979,90(2):525-530
Bovine muscle pyruvate kinase was inactivated by treatment with trinitrobenzenesulfonic acid; approximately one trinitrophenyl group was incorporated per subunit. ADP or Mg-ADP decreased the rate of inactivation but Mg++ alone or phosphoenolpyruvate had no effect. The inactivated protein was treated with trypsin and the trinitrophenylated peptide isolated by gel filtration. Homogeneity of the isolated peptide was shown by high voltage electrophoresis and high pressure liquid chromatography. Amino acid analysis and sequence determination revealed the presence of an acidic peptide 34 amino acids long and containing ?-trinitrophenylated lysine. 相似文献
179.
When added alone, the arylamine procarcinogens N-acetyl-aminofluorene, 4-acetyl-aminobiphenyl or their N-hydroxy derivatives failed to alter partially purified soluble guanylate cyclase from rat liver or particulate guanylate cyclase activity from colonic mucosa. However, addition of linoleic acid hydroperoxide to the enzyme preparation in the presence N-OH-acetyl-aminofluorene or N-OH-acetyl-aminobiphenyl significantly increased guanylate cyclase activity. With linoleic acid hydroperoxide plus N-OH-acetyl-aminofluorene, both the activation of hepatic guanylate cyclase and the formation of the carcinogen oxidation product 2-nitrosofluorene required hematin but not molecular O2. Both processes were inhibited by ascorbic acid. These data strongly imply that guanylate cyclase activation was dependent upon hematin catalyzed oxidation of N-OH-acetyl-aminofluorene by the lipid peroxide. The results provide the first evidence that guanylate cyclase activation can occur during the conversion of a procarcinogen to a more reactive chemical species, and thereby emphasize the importance of examining carcinogen interaction with the GC system under conditions which permit such chemical conversion. 相似文献
180.