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981.
Two new alkaloids, calpurmenine (12β,13α-dihydroxylupanine) and its 13α-pyrrolylcarboxylic acid ester have been isolated from a South African sample of Calpurnia aurea. The alkaloid 10,13-dihydroxylupanine, earlier found in Cadia purpurea but absent from Ethiopian material of Calpurnia aurea, was also identified. 相似文献
982.
9-O-(Pentafluorophenylcarbamoyl)quinine and 10-(pentafluorophenylcarbamoyloxy)-6'-methoxy-11-norcinchonan-9-ol, obtained by derivatization of alkaloid double bond, were compared in NMR enantiodiscrimination experiments of selected chiral substrates. 相似文献
983.
Shang-Hua Zhai Ya-Nan Lu Cheng-Qi Fan Sheng-Nan Shao 《Biochemical Systematics and Ecology》2011,39(2):161-162
Highlights? Ascidia longistriata was collected for secondary metabolites investigation. ? This is the first report of compounds 1-3 from ascidians of the genus Ascidia. ? Compound 2 might be derived from an associated organism. 相似文献
984.
Bo Sun Ling Li Shengming Wu Qi Zhang Haijing Li Hebing Chen Famei Li Fangting Dong Xianzhong Yan 《Analytical biochemistry》2009,395(2):125-133
The Aconitum alkaloids aconitine, mesaconitine, and hypaconitine are the main toxic components in a commonly used traditional Chinese herbal medicine Fu Zi. To provide guidelines for the safe use of this medicine, metabolic changes in Wistar rats caused by these compounds were investigated by means of integrated analysis of two metabonomic approaches: 1H nuclear magnetic resonance (NMR) and gas chromatography/time-of-flight mass spectrometry (GC/TOF–MS). Rats were given a single dose of aconitine, mesaconitine, hypaconitine, or vehicle. The largest metabolic changes were observed 6 h after treatment. Every group receiving a dose had higher urine concentrations of glucose, acetate, dimethylglycine, succinate, and alanine and had lower concentrations of creatinine, citrate, 2-oxoglutarate, N-acetylated metabolites, and trimethylamine-N-oxide (TMAO) than did the control group. These results may reflect the perturbation of renal tubular function within the first 24 h after treatment. The results also revealed a larger perturbation of metabolic profiles in the aconitine group than in the mesaconitine and hypaconitine groups, illustrating how these alkaloids exhibit different toxicities. An analysis of plasma samples collected 7 days postdose showed that there were higher levels of lactate, alanine, and lipids along with lower levels of glucose, β-hydroxybutyrate, and creatine in the plasma of the aconitine and mesaconitine groups than there were in the control and hypaconitine groups. The GC/TOF–MS data from the plasma samples showed that the number of metabolites, with significant changes or with a tendency to change, in the aconitine and mesaconitine groups were dissimilar, suggesting a possible difference in the acute toxicity mechanisms of these alkaloids. 相似文献
985.
2,3-Secofernane triterpenoids, alstonic acids A (1) and B (2), were isolated from the leaves of Alstonia scholaris together with an indole alkaloid, N1-methoxymethyl picrinine (3). Their structures were established from MS and NMR spectroscopic analyses and confirmed by single crystal X-ray diffraction analysis. 相似文献
986.
C. M. Maragos 《Mycotoxin Research》2009,25(2):67-75
Photochemical reaction of the non-fluorescent mycotoxin cyclopiazonic acid (CPA) to fluorescent products was recently reported.
Because CPA contains an indole moiety, believed to contribute to the fluorescence, it was of interest to determine whether
the effect might be more generally applicable to indole-containing mycotoxins. Three indole-containing tremorgens (penitrem
A, paxilline, verruculogen) that have not previously been reported to be fluorescent were rendered fluorescent by exposure
to ultraviolet light in a photoreactor. Naturally fluorescent ergot alkaloids, which also contain an indole-moiety, exhibited
a diminished response after exposure. This suggests that the phenomenon may be most useful for detection of indole-containing
tremorgens that are non-fluorescent, rather than for the enhancement of materials that are already fluorescent, such as the
ergot alkaloids. The extent to which fluorescence enhancement was seen was strongly influenced by the reaction environment,
in particular the solvent used and whether cyclodextrins were present. In an HPLC format, placement of the photoreactor post-column
allowed for the fluorescence detection of penitrem A, paxilline, and verruculogen. The ability to photoreact indole-containing
tremorgens and detect them by fluorescence may open up new avenues for detection of these mycotoxins alone or in combination. 相似文献
987.
Pyrrolizidine alkaloid (PA) clivorine, isolated from traditional Chinese medicinal plant Ligularia hodgsonii Hook, has been shown to induce apoptosis in hepatocytes via mitochondrial‐mediated apoptotic pathway in our previous research. The present study was designed to observe the protection of N‐acetyl‐cysteine (NAC) on clivorine‐induced hepatocytes apoptosis. Our results showed that 5 mM NAC significantly reversed clivorine‐induced cytotoxicity via MTT and Trypan Blue staining assay. DNA apoptotic fragmentation analysis and Western‐blot results showed that NAC decreased clivorine‐induced apoptotic DNA ladder and caspase‐3 activation. Further results showed that NAC inhibited clivorine‐induced Bcl‐xL decrease, mitochondrial cytochrome c release and caspase‐9 activation. Intracellular glutathione (GSH) is an important ubiquitous redox‐active reducing sulfhydryl (? SH) tripeptide, and our results showed that clivorine (50 µM) decreased cellular GSH amounts and the ratio of GSH/GSSG in the time‐dependent manner, while 5 mM NAC obviously reversed this depletion. Further results showed that GSH synthesis inhibitor BSO augmented clivorine‐induced cytotoxicity, while exogenous GSH reversed its cytotoxicity on hepatocytes. Clivorine (50 µM) significantly induced cellular reactive oxygen species (ROS) generation. Further results showed that 50 µM Clivorine decreased glutathione peroxidase (GPx) activity and increased glutathione S transferase (GST) activity, which are both GSH‐related antioxidant enzymes. Thioredoxin‐1 (Trx) is also a ubiquitous redox‐active reducing (? SH) protein, and clivorine (50 µM) decreased cellular expression of Trx in a time‐dependent manner, while 5 mM NAC reversed this decrease. Taken together, our results demonstrate that the protection of NAC is major via maintaining cellular reduced environment and thus prevents clivorine‐induced mitochondrial‐mediated hepatocytes apoptosis. J. Cell. Biochem. 108: 424–432, 2009. © 2009 Wiley‐Liss, Inc. 相似文献
988.
989.
990.
本文主要结合近几年的国内外研究报道,综述了中国传统中草药-两面针的化学成分、药理活性及临床疗效等方面的研究进展;并结合作者所在课题组的研究成果,重点介绍了两面针生物碱及其金属配合物的抗肿瘤活性研究。 相似文献