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71.
Abstract: The tapetum surrounds the developing sporogenous tissue, and therefore is suggested to have an important role during sporogerlesis. In pteridophytes details about tapetum development and function during sporangium development are poorly known. The current article analyses the leptospor-angiate development of the pteridophyte Cyrtomium falcatum in terms of functional compartmentalization related to tapetum function. Morphology, development and localization of different compounds during sporangium development are studied. Dur ing C. falcatum leptosporangiate development phases of initia tion, division, differentiation, and maturation are identified. Functional compartments are distinguished by the presence of a callose culture sac during the division and differentiation phase, and a callose spore sac during the differentiation and ma turation phase. 相似文献
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Magnesium Transporter 5 plays an important role in Mg transport for male gametophyte development in Arabidopsis
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Xiao‐Feng Xu Bo Wang Yue Lou Wen‐Jian Han Jie‐Yang Lu Dan‐Dan Li Le‐Gong Li Jian Zhu Zhong‐Nan Yang 《The Plant journal : for cell and molecular biology》2015,84(5):925-936
During anther development the male gametophyte develops inside the locule and the tapetal cells provide all nutrients for its development. Magnesium Transporter 5 (MGT5) is a member of the MGT family and has dual functions of Mg export and import. Here, we show that male gametophyte mitosis and intine formation are defective in a mgt5 mutant. The transient expression of GFP‐MGT5 revealed that MGT5 is localized in the plasma membrane. These findings suggest that in the male gametophyte MGT5 plays a role in importing Mg from the locule and that Mg is essential for male gametophyte development. The expression of MGT5 in the knockout ABORTED MICROSPORES (AMS) mutant (AMS being an essential regulator of tapetum) is tremendously reduced. Chromatin immunoprecipitation and mobility shift assay experiments demonstrated that AMS can directly bind the promoter of MGT5. An immunoelectron microscopy assay revealed that MGT5‐His is localized to the plasma membrane of the tapetum. These findings suggest that AMS directly regulates MGT5 in the tapetum and thus induces export of Mg into the locule. The mgt5 plant exhibits severe male sterility while the expression of MGT5 under the tapetum‐specific promoter A9 partly rescued mgt5 fertility. mgt5 fertility was restored under high‐Mg conditions. These findings suggest that the mgt5 tapetum still has the ability to export Mg and that a sufficient supply of Mg from the tapetum can improve the importation of Mg in the mgt5 male gametophyte. Therefore, MGT5 plays an important role in Mg transport from the tapetum to the microspore. 相似文献
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75.
BACKGROUND AND AIMS: Arum alpinum has a quite uncommon pollen wall. A sporopolleninous ektexine is missing. The outermost pollen wall layer is formed by the endexine which is covered by polysaccharidic ornamentation elements. An ontogenetical investigation was accomplished to clarify pollen-wall development, with special reference to callose and pollen-wall development. METHODS: Plants of Arum alpinum grown in their natural habitat were collected once a week within the vegetative period and processed for semi- and ultra-thin sectioning. KEY RESULTS: At any stage of pollen-wall formation callose is missing. Microspores are released from the tetrad by invagination of the amoeboid tapetum. The polysaccharidic wall ornamentations are formed by the tapetum. CONCLUSIONS: There appears to be no truth in the dogma that callose is essential for microspore separation and release from the tetrad. The lack of callose does not influence fertility but could be the reason for the uncommon pollen wall, where a sporopolleninous ektexine is missing. 相似文献
76.
González-Melendi P Uyttewaal M Morcillo CN Hernández Mora JR Fajardo S Budar F Lucas MM 《Journal of experimental botany》2008,59(4):827-838
Ogura cytoplasmic male sterility (CMS) occurs naturally in radishand has been introduced into rapeseed (Brassica napus) by protoplastfusion. As with all CMS systems, it involves a constitutivelyexpressed mitochondrial gene which induces male sterility tootherwise hermaphroditic plants (so they become females) anda nuclear gene named restorer of fertility that restores pollenproduction in plants carrying a sterility-inducing cytoplasm.A correlative approach using light and electron microscopy wasapplied to define what stages throughout development were affectedand the subcellular events leading to the abortion of the developingpollen grains upon the expression of the mitochondrial protein.Three central stages of development (tetrad, mid-microsporeand vacuolate microspore) were compared between fertile, restored,and sterile plants. At each stage observed, the pollen in fertileand restored plants had similar cellular structures and organization.The deleterious effect of the sterility protein expression startedas early as the tetrad stage. No typical mitochondria were identifiedin the tapetum at any developmental stage and in the vacuolatemicrospores of the sterile plants. In addition, some strikingultrastructural alterations of the cell's organization werealso observed compared with the normal pattern of development.The results showed that Ogu-INRA CMS was due to premature celldeath events of the tapetal cells, presumably by an autolysisprocess rather than a normal PCD, which impairs pollen developmentat the vacuolate microspore stage, in the absence of functionalmitochondria. Key words: Brassica napus, cell death, light and electron microscopy, mitochondria, plastids, pollen development, Ogu-INRA cytoplasmic male sterility, transgenic-restored plants, tapetum
Received 30 September 2007; Revised 11 December 2007 Accepted 20 December 2007 相似文献
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Giuseppina Falasca Simone D'Angeli Rita Biasi Laura Fattorini Maja Matteucci Antonella Canini Maria Maddalena Altamura 《Annals of botany》2013,112(6):1045-1055
Background and Aims
Dioecism characterizes many crop species of economic value, including kiwifruit (Actinidia deliciosa). Kiwifruit male sterility occurs at the microspore stage. The cell walls of the microspores and the pollen of the male-sterile and male-fertile flowers, respectively, differ in glucose and galactose levels. In numerous plants, pollen formation involves normal functioning and degeneration timing of the tapetum, with calcium and carbohydrates provided by the tapetum essential for male fertility. The aim of this study was to determine whether the anther wall controls male fertility in kiwifruit, providing calcium and carbohydrates to the microspores.Methods
The events occurring in the anther wall and microspores of male-fertile and male-sterile anthers were investigated by analyses of light microscopy, epifluorescence, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL assay) and transmission electron microscopy coupled with electron spectroscopy. The possibility that male sterility was related to anther tissue malfunctioning with regard to calcium/glucose/galactose provision to the microspores was also investigated by in vitro anther culture.Key Results
Both tapetum and the middle layer showed secretory activity and both degenerated by programmed cell death (PCD), but PCD was later in male-sterile than in male-fertile anthers. Calcium accumulated in cell walls of the middle layer and tapetum and in the exine of microspores and pollen, reaching higher levels in anther wall tissues and dead microspores of male-sterile anthers. A specific supply of glucose and calcium induced normal pollen formation in in vitro-cultured anthers of the male-sterile genotype.Conclusions
The results show that male sterility in kiwifruit is induced by anther wall tissues through prolonged secretory activity caused by a delay in PCD, in the middle layer in particular. In vitro culture results support the sporophytic control of male fertility in kiwifruit and open the way to applications to overcome dioecism and optimize kiwifruit production. 相似文献79.
D. EDWARDS L. AXE E. MENDEZ 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(3):297-310
A new taxon Sporathylacium salopense gen. et sp. nov . is based on small isolated coalified sporangia from the Lower Devonian (Lochkovian: micrornatus—newportensis spore zone) from the Welsh Borderland. The sporangia have two equal valves with multi-layered walls and thickened borders, and contain trilete crassitate, non-curvaturate isospores that are completely covered by a microgranular ornament with possible distal verrucate/murornate structures. They differ from zosterophyll sporangia in details of the presumed dehiscence zone extending around the entire free convex margin, particularly in the presence of a wedge of amorphous material between the valves, and in spore characters. Absence of any information relating to water-conducting cells prevents further assignment within the embryophytes. Spherical bodies associated with spores and a resilient sporangial lining are compared with similar structures in extant free-sporing plants and with Ubisch bodies. The mode of sporangial dehiscence involving anatomical modifications of the valve margins and the novel wedge of tissue which connects them remains speculative. 相似文献
80.
Benjamin Eggs Jonas O. Wolff Lucia Kuhn‐Nentwig Stanislav N. Gorb Wolfgang Nentwig 《Ethology : formerly Zeitschrift fur Tierpsychologie》2015,121(12):1166-1177
More than half of all spider species hunt prey without a web. To successfully subdue their prey, they use adapted capture behaviour and efficient grasping mechanisms to interrupt the prey's locomotion, and to restrain it from escaping during the subsequent handling for final envenomation. In this study, we investigated how the prey capture behaviour of different lycosoid spider species is related to leg morphology and venom efficiency; using high speed videography, feeding experiments, stereomicroscopy, scanning electron microscopy and LD50 venom bioassays. We found that different species employed different techniques when grasping their prey and these differences strongly correlate with the distribution and size of hairy adhesive leg pads (so‐called scopulae on pro‐ and retrolateral parts of legs) and erectable spines, which act in a complementary way. Our results indicate that the grasping and handling behaviour and leg morphology is crucial in restricting the prey's movements. However, none of these traits is directly related with venom efficiency. 相似文献