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991.
振动光谱与蛋白质二级结构 总被引:3,自引:0,他引:3
红外光谱和拉曼光谱(统称分子振动光谱)应用于蛋白质构象研究经历了定性阶段和半定量阶段.近5年来,去卷积和二阶导教等提高分辨率方法以及曲线拟合方法的应用,使得振动光谱在定量计算蛋白质二级结构和监测蛋白质构象变化等方面取得突破性的进展,进入了定量化的阶段. 相似文献
992.
Martin J. Koch Hans K. Biesalski Eckart Stofft Harald Weiser Helmut E. Gabbert Hans-P. Dienes Burkard Schulz-Dobrick Karl H. Bässler 《Cell and tissue research》1990,260(3):625-628
Summary The effect of vitamin A-deficiency on jejunal Paneth cells in rats was investigated. Crystalloid particles were observed in secretion granules of Paneth cells from 6 out of 8 rats with vitamin A-deficiency. The particles were similar to those found in Paneth cells under other experimental conditions. Using an immuno-electron-microscopic technique we demonstrated a clear lysozyme immunoreactivity of these particles. In 2 vitamin A-deficient rats tubular structures have been detected in addition to the crystalloid particles. Crystalloid particles or tubular structures were not detectable in a control group of 8 vitamin A-supplemented rats. The morphological alterations of Paneth cells may be correlated to an impaired local immunity of the intestine during vitamin A-deficiency.This work represents a portion of a doctoral thesis presented by M.J. Koch in partial fullfillment for the degree of medical doctor 相似文献
993.
The structures of five basic pancreatic trypsin inhibitor (BPTI) molecules are compared to establish the extent and nature of the conformational variability resulting from crystal packing effects. BPTI is an ideal system to evaluate such factors because of the availability of high resolution X-ray models of five different BPTI structures, each in a different crystal packing environment. Differences observed among the structures are found to be distributed throughout the molecule, although the regions that display most variability are associated with the loop structures (residues 14-17 and 24-29). The regions of structure that show the largest rms deviations from the mean of the five packing motifs correlate well with the presence of intermolecular contacts in the crystal lattice. For most of the molecules there is also a correspondence between a larger number of intermolecular contacts and systematically higher B-factors, although it is not apparent whether this is induced by the crystal contact or results from the fact that the contacts are made predominantly through surface loops. The conformational differences seen among the X-ray models constitute more than local shifts at the lattice contact surfaces, and in fact involve in some cases the making and breaking of intramolecular H-bonds. The magnitudes of the differences among packing models are significantly larger than those usually associated with changes induced by mutagenesis; for instance; the structural differences at the site of mutation observed on removing an internal disulfide from the molecule are significantly less than those associated with lattice contact effects. The crystal packing conformations are compared to representative structures of BPTI generated during a 96-psec molecular dynamics (MD) simulation. This comparison shows a high level of correspondence between the protein flexibility indicated by the X-ray and MD analyses, and specifically between those regions that are most variable. This suggests that the regions that show most variability among the crystal packing models are not artifacts of crystallization, but rather represent true low-energy conformers that have been preferentially selected by crystallization factors. 相似文献
994.
D. Bassolino-Klimas R. E. Bruccoleri S. Subramaniam 《Protein science : a publication of the Protein Society》1992,1(11):1465-1476
A model structure has been constructed for a monoclonal anti-dinitrophenyl antibody. The antibody, ANO2, has been sequenced and cloned (Anglister, J., Frey, T., & McConnell, H.M., 1984, Biochemistry 23, 1138-1142). Its amino acid sequence shows striking homology with the anti-lysozyme Fab fragments HyHel5 (83%) and HyHel10 (73%). Based on this homology, a model for the ANO2 variable heavy and variable light chain framework was constructed using a hybrid of the HyHel5 light chain and the HyHel10 heavy chain backbone, omitting the hypervariable loops. These coordinates were used as scaffolds for the model building of ANO2. The CONGEN conformational sampling algorithm (Bruccoleri, R.E. & Karplus, M., 1987, Biopolymers 26, 127-196) was used to model the six hypervariable loops that contain the antigen-combining site. All the possible conformations of the loop backbones were constructed and the best loop structures were selected using a combination of the CHARMM potential energy function and evaluation of the solvent-accessible surface area of the conformers. The order in which the loops were searched was carried out based on the relative locations of the loops with reference to the framework of the beta-barrel, namely, L2-H1-L3-H2-H3-L1. The model structures thus obtained were compared to the high resolution X-ray structure (Brünger, A.T., Leahy, D.J., Hynes, T.R., & Fox, R.O., 1991, J. Mol. Biol. 221, 239-256). 相似文献
995.
Samar K. Kundu Yadollah Harati Lalith K. Misra 《Biochemical and biophysical research communications》1984,118(1):83-89
Gangliosides of healthy and pathologic muscles (amyotropic lateral sclerosis and facio-scapulo-humeral muscular dystrophy) were studied. Total ganglioside content of the affected muscles was approximately 2 fold higher than the unaffected muscles. Our results showed that ALS muscle contained a ganglioside which was absent in the unaffected and FSH muscular dystrophic muscles. Based on the results of hydrolysis with neuraminidase and subsequent reactivity of the asialo derivative towards anti-globotetraosylceramide, we propose that the ALS ganglioside is sialosylglobtetraosylceramide, NeuAc(α2–3)GalNAc(β1–3)Gal(α1–4)Gal(β1–4)Glc-Cer. 相似文献
996.
997.
Formation of Uniform N‐doped Carbon‐Coated SnO2 Submicroboxes with Enhanced Lithium Storage Properties
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Rational design and preparation of SnO2‐based materials with superior electrochemical performance for lithium‐ion batteries are highly desirable. In this work, the synthesis of SnO2/nitrogen‐doped carbon (SnO2/NC) submicroboxes with excellent lithium storage properties is reported. The as‐synthesized SnO2/NC submicroboxes are highly porous with a high specific surface area of 125 m2 g?1, well‐defined hollow structure (around 400 nm in size) with a shell thickness of 40 nm, and ultrasmall SnO2 nanoparticles uniformly coated with nitrogen‐doped carbon layer. As a result, the SnO2/NC submicroboxes show outstanding electrochemical performance as an anode material for lithium‐ion batteries. A high reversible capacity of 491 mAh g?1 can be retained after 100 cycles at a current density of 0.5 A g?1. 相似文献
998.
Bond valence sums and structural studies of antimony complexes containing Sb bonded only to O ligands 总被引:1,自引:0,他引:1
Ruth C. Palenik 《Inorganica chimica acta》2005,358(4):1034-1040
New R0 values for Sb(III)-O of 1.955(13) Å and for Sb(V)-O of 1.912(12) Å were derived from a bond valence sum, or BVS, analysis of of 123 SbOn fragments with n = 3-7. These R0 values can be used to calculate the oxidation state, or OS, of Sb in complexes where Sb is bonded only to O ligands. If the OS of the Sb ion is uncertain, the average R0 value of 1.934 Å can be used to decide between the +3 or +5 OS from the observed Sb-O bond distances without any assumptions. The Sb-O distances used in deriving the R0 values for the +3 and +5 complexes are tabulated as a function of coordination number and have a wide range of values, but the average Sb-O distance reflects the requirement that the BVS must equal the OS. To supplement the Sb-O data, 2 new antimony tartrates were synthesized, and their crystal structures were determined. A single crystal structure determination of NaSb(OH)6, an unexpected reaction product, gave Sb-O distances that were also included in our BVS analysis. 相似文献
999.
James Cookson Emma A.L. Evans Christopher J. Serpell Andrew R. Cowley Paul D. Beer 《Inorganica chimica acta》2010,363(6):1195-2710
The synthesis of a range of dinuclear Cu(II) dithiocarbamate (dtc)-based macrocycles and their characterisation are described. By carefully tuning the size of the aromatic spacer, cavities of different dimensions can be designed. The length and flexibility of the chosen spacer group dictates the intermetallic distance and hence the degree of communication between the two metal centres as evidenced by electrochemical and EPR experiments. This is illustrated by crystallographic evidence that show the macrocycles can host guests (such as CH2Cl2) or can fold and form unexpected Cu(I) dtc clusters. 相似文献
1000.
《Cell reports》2020,30(3):714-724.e5
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