The fire ant social chromosome supergene variant Sb shows low diversity but high divergence from SB

Variation in social behaviour is common, yet little is known about the genetic architectures underpinning its evolution. A rare exception is in the fire ant Solenopsis invicta: Alternative variants of a supergene region determine whether a colony will have exactly one or up to dozens of queens. The two variants of this region are carried by a pair of ‘social chromosomes’, SB and Sb, which resemble a pair of sex chromosomes. Recombination is suppressed between the two chromosomes in the supergene region. While the X‐like SB can recombine with itself in SB/SB queens, recombination is effectively absent in the Y‐like Sb because Sb/Sb queens die before reproducing. Here, we analyse whole‐genome sequences of eight haploid SB males and eight haploid Sb males. We find extensive SB–Sb differentiation throughout the >19‐Mb‐long supergene region. We find no evidence of ‘evolutionary strata’ with different levels of divergence comparable to those reported in several sex chromosomes. A high proportion of substitutions between the SB and Sb haplotypes are nonsynonymous, suggesting inefficacy of purifying selection in Sb sequences, similar to that for Y‐linked sequences in XY systems. Finally, we show that the Sb haplotype of the supergene region has 635‐fold less nucleotide diversity than the rest of the genome. We discuss how this reduction could be due to a recent selective sweep affecting Sb specifically or associated with a population bottleneck during the invasion of North America by the sampled population.     

Assessing habitat utilization by neotropical primates: a new approach

This study aims to ascertain habitat utilization, in relation to forest structural variation, by a multi-species group of primates in tropical lowland rainforest in Southeast Peru during dry season. A new approach to assessing habitat utilization was used. Habitat variation was described by structural and indicator variables collected in quadrats along transects through a study area within Terra Firme and Floodplain forest. Variables were grouped into ‘factors’ accounting for most of the variation by means of a Principal Components Analysis (PCA). Presence or absence of the primates within the quadrats, assessed by repeat transect surveys, was taken to indicate habitat preferences. Discrimination between the habitat and forest structure in areas of primate presence as opposed to absence was carried out by means of Discriminant Function Analysis (DFA). This highlighted patterns in most utilized habitat. Vertical utilization of the forest was also assessed along with presence in bamboo and general activity on encounter. Suggestions of habitat preference and utilization are made for each of the six sympatric species studied, based on significantly discriminating habitat factors, vertical stratification on encounter and relationships with bamboo.Saguinus fuscicollis andCebus moloch appeared as habitat generalists.Cebus apella, Saimiri sciureus, andAotus spp., exhibited varying degrees of preference for habitat factors suggesting disturbed forest,Cebus albifrons was more generalistic but with a possible association with primary, naturally disturbed forest.C. appella was encountered in Terra Firme forest significantly more than in Floodplain. ForS. sciureus, C. moloch, andC. apella, upper understory was the most utilized forest layer, forC. albifrons, middle canopy and forS. sciureus andAotus spp., lower understory. Both positive and negative relationships with bamboo were highlighted. Significant positive relationships betweenAotus spp., and bamboo suggest dense bamboo stands provide important daytime resting sites for this nocturnal species.C. moloch was also shown to utilize bamboo whereasS. fuscicollis appeared to actively avoid it. High levels of conformity with past studies in terms of habitat utilization patterns described for the species studied supports the conclusion that the methods used in this study provide an effective means of assessing primate habitat utilization within complex habitat.     

Nutrient-limited biodegradation of PAH in various soil strata at a creosote contaminated site

The effects of nutrient addition on the in situ biodegradation of polycyclic aromatic hydrocarbons in creosote contaminated soil were studied in soil columns taken from various soil strata at a wood preserving plant in Norway. Three samples were used: one from the topsoil (0–0.5 m), one from an organic rich layer (2–2.5 m) and one from the sandy aquifer (4.5–5 m). The addition of inorganic nitrogen and phosphorous stimulated the degradation of polycyclic aromatic hydrocarbons (PAHs) in the top soil and the aquifer sand. These two soils, which differed strongly in contamination levels, responded similarly to nutrient addition with the corresponding degradation of 4-ring PAHs. The ratio between available nitrogen (N) and phosphorous (P) might explain the degree of degradation observed for the 4-ring PAHs. However, the degree of degradation of 3-ring PAHs did not significantly increase after nutrient addition. An increase in the respiration rate, after nutrient addition, could only be observed in the topsoil. In the aquifer sand, 4-ring PAH degradation was not accompanied by an increase in the respiration rate or the number of heterotrophic micro-organisms. PAH degradation in the organic layer did not respond to nutrient addition. This was probably due to the low availability of the contaminants for micro-organisms, as a result of sorption to the soil organic matter. Our data illustrate the need for a better understanding of the role of nutrients in the degradation of high molecular weight hydrocarbons for the successful application of bioremediation at PAH contaminated sites.     

Use of space by the marsupial Marmosops incanus (Didelphimorphia, Didelphidae) in the Atlantic Forest, Brazil

The biology of small marsupials is poorly known because most species are nocturnal and arboreal. Using the spool-and-line device, we investigated the use of vertical space by male and female Marmosops incanus in the Serra dos Órgãos National Park. Diameter and slopes of supports also were compared. The species was sexually dimorphic in body size, males weighing on average 72.6 g and females 48.1 g, but males and females did not differ in the frequency of ground movements (GM) and understory movements (UM), or in diameter and slopes of supports used. Males and females also used the forest strata similarly, moving 67% on the ground and 33% in the understory. Both sexes used 0.9–1.7 cm support diameters, and support slopes near the horizontal (more than 50% of cases), but preferred to access a given height by moving on high slope supports (mostly near 90°). Sexual dimorphism in body size does not seem to constrain movements of M. incanus.     

A new genus of lycopods from the Devonian of New York State

Drepanophycus colophyllus Grierson & Banks is transferred to llaskinsia Grierson & Banks on the basis of the morphology of its leaves and its anatomical structure. Slender, dichotomizing axes bear helically arranged leaves that are falcate, petiolate, simple, lanceolate and entire. A solid cylinder of metaxylem is surrounded by ridges of protoxylem. Pitting on tracheid walls is annular, helical, reticulate, scalariform to circular-bordered. This lycopod extends from early Givetian into early Frasnian time in the Catskill strata of eastern New York State. The effect of cleavage of fossiliferous rock on patterns observed on the exposed fossils and the bearing of recent research on the fossil history of lycopods are discussed.     

A review of the sub-species carpatica and tortuosa within the species betula pubescens Ehrh.

Summary

The classifications employed during the last decade for defining sub-specific variation within the species B. pubescens Ehrh. have produced a situation in which the terms carpatica and tortuosa, originally and usually applied to separate taxa, could be regarded as synonymous. This has been caused by the association of both terms, at different times, with B. odorata Bechst. Recent research at Merlewood supports the use of the term ssp. tortuosa and its distinction from ssp. carpatica whose presence in the British flora requires further verification.     

Sexual niche partitioning in two species of New Guinean Pachycephala whistlers

Sex differences in foraging behavior have been widely reported in the ornithological literature, but few examples are available from tropical avifaunas. Differences between males and females in foraging behavior have been hypothesized to be a byproduct of sexual size dimorphism or a result of niche partitioning to reduce intersexual competition for food or different reproductive roles. From 2010 to 2013, I used foraging data and mist‐net capture rates from multiple study sites to examine possible sex differences in the foraging behavior of two New Guinean Pachycephala whistlers. I found that male Regent (Pachycephala schlegelii) and Sclater's (Pachycephala soror) whistlers consistently foraged in higher strata than females. It is unlikely that these differences are due to sexual dimorphism because these species exhibit little sexual dimorphism. Sex differences in foraging behavior were consistent across years and study sites and did not appear linked to breeding behavior, supporting the food‐competition hypothesis, but not the reproductive‐roles hypothesis. Male territorial defense often occurs in relatively high strata in Pachycephala whistlers, possibly influencing male foraging strata. However, male territorial behavior cannot explain why females predominately forage in lower strata. Instead, intersexual competition for food resources is likely the primary driver of differences in the foraging behavior of male and female Regent and Sclater's whistlers.     

Morphology and microanatomy of harbor porpoise (Phocoena phocoena) dorsal fin tubercles

The unique pattern of small tubercles on the leading edge of the dorsal fins of harbor porpoises (Phocoena phocoena) has been widely noted in the literature, though their structure or function has never been conclusively identified. We examined external morphology and microanatomy of the tubercles for further understanding of the nature of the tubercles. Measurements were taken of height and peak‐to‐peak distance of the tubercles using scaled photographs. Mean tubercle height was standardized as a percentage of the dorsal fin height and ranged from 0.63 to 0.87%. Mean peak‐to‐peak distance ranged from 4.2 ± 2.0 to 5.6 ± 2.0 mm. The microstructure analysis of the dorsal fin leading edge, trailing edge and tubercles revealed an epidermal thickness of 0.7–2.7 mm with the thickest epidermis at the tubercular apex. The epidermis contained three distinct strata (=layers), including the stratum corneum, spinosum, and basale. The stratum corneum was significantly thickened in tubercles, over four times thicker than in the leading or trailing edge of the fin. The stratum spinosum, composed of lipokeratinocytes and lamellar oil bodies, was significantly thinner in the trailing edge than in the other two sites. There was no significant difference in the stratum basale among the three sites. Volume fraction of lipokeratinocytes was significantly higher at the sides of the leading edge and the apex of the tubercles, while volume fraction of lamellar oil bodies was significantly lower at the apex of the tubercles. Though the function of the tubercles is unknown, their position, hardened structure and increased epidermal stratum corneum suggest that they may have hydrodynamic importance. J. Morphol., 2011. © 2010 Wiley‐Liss, Inc.     

Indicating assemblage vulnerability and resilience in the face of climate change by means of adult ground beetle length–weight allometry over elevation strata in Tenerife (Canary Islands)

Adaptive trade-offs in length–weight allometry might reduce vulnerability under climate change of adult ground beetle (Col., Carabidae) assemblages in their original elevation stratum on Tenerife. In particular this study shows that the predictive values for simple log-linear regression parameters were high in all strata and also the F-tests were statistically significant (p < 0.0001). Ground beetle assemblage on upper stratum had smaller coefficients and higher intercepts than did lower ones, indicating that the ground-beetle assemblages may be trading off higher powers for higher resilience via water and thermal efficiency in the face of environmental warming, in opposition to strategies adopted in cool and wet climates. Adults of ground beetle assemblages from warm and dry lower strata might have to be heftier, with encapsulation of bodies and heavily sclerotized exoskeleton, than those from cloudy, cool and wet strata; the latter group, freed from this constraint, would thus be characterized by more elongated, thinner and softer-bodied species. The outlined methodology could become a useful tool for the vulnerability and resilience assessment of natural assemblages, and could theoretically be applied to any latitudinal and altitudinal assemblage.