Patterns of co-occurrence among arboreal marsupials in the forests of central Victoria,southeastern Australia

Abstract Patterns of co-occurrence were examined among the suite of species of arboreal marsupials that inhabit the montane ash forests of the Central Highlands of Victoria, southeastern Australia. The data used in our analyses were counts of different species of arboreal marsupials derived from stagwatching surveys of 152 sites throughout the Central Highlands region. Our work investigated both the number of species that tend to occur together, as well as identity of the taxa in such associations. A total of eight different species of arboreal marsupials were recorded from field surveys of montane ash forests: Leadbeater's Possum (Gymnobelideus leadbeateri), Sugar Glider (Petaurus breviceps), Yellow-bellied Glider (Petaurus australis), Greater Glider (Petauroides volans), Mountain Brushtail Possum (Trichosurus caninus), Feathertail Glider (Acrobates pygmaeus), Common Ringtail Possum (Pseudocheirusperegrinus), and Eastern Pygmy Possum (Cercartetus nanus). Large assemblages of taxa were rare and no sites supported the full array of species of arboreal marsupials– the maximum number of species recorded on a given 3 ha site was five. The results of our analyses indicated that if any one species of arboreal marsupials was present at a site, then on average 1. 5 other taxa will co-inhabit that site. The exception was the Common Ringtail Possum. When this species was detected, few other arboreal marsupial taxa tended to be recorded with it. Principal Co-ordinates Analysis showed that although combinations of animals comprised of either Mountain Brushtail Possum, Leadbeater's Possum, or Greater Glider were often recorded, there were no strong patterns in the identities of co-occurring taxa. This finding, together with the result that most taxa in these forests typically co-occurred with one or two other species, indicates that none of the arboreal marsupials inhabiting the montane ash forests of the Central Highlands of Victoria would be a good surrogate for (or ‘indicator’ of), the presence of the suite of other species in the assemblage.     

Whole exome sequencing,in silico and functional studies confirm the association of the GJB2 mutation p.Cys169Tyr with deafness and suggest a role for the TMEM59 gene in the hearing process

The development of next generation sequencing techniques has facilitated the detection of mutations at an unprecedented rate. These efficient tools have been particularly beneficial for extremely heterogeneous disorders such as autosomal recessive non-syndromic hearing loss, the most common form of genetic deafness. GJB2 mutations are the most common cause of hereditary hearing loss. Amongst them the NM_004004.5: c.506G > A (p.Cys169Tyr) mutation has been associated with varying severity of hearing loss with unclear segregation patterns. In this study, we report a large consanguineous Emirati family with severe to profound hearing loss fully segregating the GJB2 missense mutation p.Cys169Tyr. Whole exome sequencing (WES), in silico, splicing and expression analyses ruled out the implication of any other variants and confirmed the implication of the p.Cys169Tyr mutation in this deafness family. We also show preliminary murine expression analysis that suggests a link between the TMEM59 gene and the hearing process. The present study improves our understanding of the molecular pathogenesis of hearing loss. It also emphasizes the significance of combining next generation sequencing approaches and segregation analyses especially in the diagnosis of disorders characterized by complex genetic heterogeneity.     

Regulation of C4 photosynthesis: characterization of a protein factor mediating the activation and inactivation of NADP-malate dehydrogenase.

The leaf NADP-malate dehydrogenase of Zea mays is rapidly activated when leaves are illuminated and inactivated in the dark. The present studies show that inactive enzyme isolated from darkened leaves was activated by dithiothreitol and that the active enzyme was rapidly inactivated by oxygen in dithiothreitol-free solutions. Following the fractionation of leaf extracts, both the activation and inactivation of NADP-malate dehydrogenase in vitro were partially or totally dependent upon a separate small molecular weight protein factor. Activation and inactivation were largely or solely dependent upon this factor at pH 8.0 or less, but apparently only partially factor dependent at pH 9.0. The factor was heat stable, inactivated by incubation with trypsin, and had a molecular weight of about 10,000. It was mostly associated with the chloroplasts of mesophyll cells.     

Characterisation of gut hormones storage granules from normal human jejunum using metrizamide density gradients

Analytical subcellular fractionation techniques using metrizamide density gradients have been used to investigate the properties of the gut hormone storage granules and the principal organelles from homogenates of normal human jejunal mucoosa obtained by peroral mucosal biopsy. The individual hormones, detected by radioimmunoassay, each showed single discrete peaks in the density gradient experiments indicating localisation to single granules each with characteristic modal densities. Thus motilin showed a modal density of 1.15, gastrin 1.16, gastric inhibitory polypeptide (GIP) 1.17, enteroglucagon 1.18 and somatostatin and vasoactive intestinal peptide (VIP) 1.10 g/ml. The following organelles, characterised by their marker enzymes were located in the density gradients; plasma membrane (5′-nucleotidase) brush border (α-glucosidase, pH 6.0) mitochondria (particulate malate dehydrogenase), peroxisomes (catalase), lysosomes ($\text{N-}\text{acetyl-β-glucosaminidase}$), endoplasmic reticulum (α-glucosidase, pH 8.0), cytosol (lactate dehydrogenase). These studies provide biochemical evidence of the distinct nature of the individual gut hormone storage granules and provide a basis for studying dynamic changes in the granules in response to physiological stimuli and pathological processes.     

SARS-CoV-2 spike variants exhibit differential infectivity and neutralization resistance to convalescent or post-vaccination sera

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Convergence of Differentially Invaded Systems toward Invader-dominance: Time-lagged Invasions as a Predictor in Desert Fish Communities

To what extent do patterns of invasion in one region generalize to other regions? Answering this question is a key goal of invasion biology because it underlies whether we can make progress via comparative studies or must instead cope with a large set of unique cases. Here we quantify similarities and differences in the historical development of nonnative fish assemblages of two North American desert drainages, one with many nonnative fishes (Gila Basin, principally southwestern USA), and one with few (Yaqui Basin, principally northwestern Mexico). The two river basins are similar in size, physiography, and ecology, but because of differences in the timing of regional development, we hypothesized that richness and geographic spread of nonnative fishes in the Yaqui are time-lagged relative to the Gila, and that a slow, but steady increase of nonnative fish occurrence is underway in the Yaqui, similar to what has already occurred in the Gila. Using the comprehensive SONFISHES database, we found that increases in the regional richness of extant nonnative species over time have been roughly linear in both basins. Meanwhile, previously established species have continued to spread spatially, such that the cumulative number of reach records for nonnative species has increased roughly exponentially in both systems. The current status of nonnatives in the Yaqui is remarkably similar to what was evident from the Gila in the past at a comparable level of sampling effort. For all measures of invasion dynamics we examined, a time lag of ～ 40–50 years exists between the Gila and Yaqui. The majority of extant nonnative fishes are known piscivores, and many have high levels of parental care, a life history trait that affords considerable advantages over native fishes. These results predict that, absent strong action now, the presently abundant native fish fauna of the Yaqui may become increasingly imperiled over the next several years, with a future similar to the Gila, where most native fishes are either extirpated, threatened, or substantially reduced in range, at least partially due to nonnative fishes. We recommend immediate actions to identify and protect high priority portions of the Yaqui Basin from further nonnative fish invasion before further degradation occurs.     

Early Predictors of Gestational Diabetes Mellitus in IVF-Conceived Pregnancies

ObjectiveGestational diabetes mellitus (GDM) is associated with adverse maternal and fetal outcomes. This study aimed to identify early and reliable GDM predictors that would enable implementation of preventive and management measures.MethodsThe participants were a 28-week prospective cohort of in vitro fertilization (IVF)-conceived pregnant women (≤39 years, body mass index [BMI] 18.5-38 kg/m²) without a known history of diabetes mellitus. Fasting blood samples were analyzed at baseline (pre-IVF) and 12 weeks’ gestation for reproductive hormones, glucose, serum insulin, lipids, thyroid function, adiponectin, and lipopolysaccharide-binding protein. At 28 weeks, a 75-g oral glucose tolerance test was used to screen for GDM.ResultsFor the overall group at baseline, 22% had BMI ≥30 kg/m², 45% had polycystic ovary syndrome, 16% had hemoglobin A1C of 5.7% to 6.1%, and 14% had a past history of GDM. At 28 weeks of gestation (n = 158), 34 women had developed GDM and 124 had not. Significant baseline predictors of GDM onset included greater BMI (29.0 vs 25.8 kg/m²), older age (34 vs 32 years), higher levels of follicle-stimulating hormone/luteinizing hormone ratio (1.2 vs 1.0), hemoglobin A1C (5.5 vs 5.2%), insulin (10.6 vs 7.1 μIU/mL), homeostatic model assessment of insulin resistance (2.2 vs 1.7), total cholesterol (199 vs 171 mg/dL), and low-density lipoprotein cholesterol (123 vs 105 mg/dL), and lower triglyceride levels (74 vs 76 mg/dL). Significant 12-week GDM predictors included greater maternal weight gain (delta: 3.4 vs 1.5 kg) and higher levels of insulin (11.3 vs 7.6 μIU/mL), triglycerides (178 vs 120 mg/dL), and homeostatic model assessment of insulin resistance (2.3 vs 1.5). Twelve-week BMI is a predictor of GDM following adjustment for polycystic ovary syndrome status and maternal age.ConclusionWhile preconception maternal BMI, age, and follicle-stimulating hormone/luteinizing hormone ratio are predictors of subsequent development of GDM, early IVF-conceived gestational weight gain is the best predictor of GDM onset.     

The occurrence of ixodid ticks on wild mink Mustela visonin England and Wales

Abstract. Four species of ticks found to infest 1391 American mink (Mustela vison) in Britain in five years were, in declining order of frequency, Ixodes hexagonus, Ixodes canisuga, Ixodes ricinus and Ixodes acuminatus. Ixodes hexagonus and I.canisuga occurred on 40% and 2.5% of mink respectively. Infestation rates (the proportion of infested mink) of adult females, nymphs and larvae were similar and tended to be lower in summer. The distribution of infestation size (the number of ticks per host) for adult females describes a negative binomial. The mean infestation size of nymphs varied with the sex of the host and was 5.2 for males and 4.2 for females. Mink are competent hosts for /. hexagonus.