全文获取类型
收费全文 | 17351篇 |
免费 | 1176篇 |
国内免费 | 735篇 |
专业分类
19262篇 |
出版年
2024年 | 68篇 |
2023年 | 325篇 |
2022年 | 535篇 |
2021年 | 635篇 |
2020年 | 592篇 |
2019年 | 675篇 |
2018年 | 656篇 |
2017年 | 467篇 |
2016年 | 496篇 |
2015年 | 631篇 |
2014年 | 790篇 |
2013年 | 1039篇 |
2012年 | 510篇 |
2011年 | 648篇 |
2010年 | 506篇 |
2009年 | 643篇 |
2008年 | 699篇 |
2007年 | 721篇 |
2006年 | 685篇 |
2005年 | 586篇 |
2004年 | 543篇 |
2003年 | 528篇 |
2002年 | 493篇 |
2001年 | 302篇 |
2000年 | 301篇 |
1999年 | 361篇 |
1998年 | 335篇 |
1997年 | 322篇 |
1996年 | 296篇 |
1995年 | 276篇 |
1994年 | 285篇 |
1993年 | 270篇 |
1992年 | 281篇 |
1991年 | 226篇 |
1990年 | 210篇 |
1989年 | 220篇 |
1988年 | 172篇 |
1987年 | 182篇 |
1986年 | 177篇 |
1985年 | 218篇 |
1984年 | 244篇 |
1983年 | 164篇 |
1982年 | 174篇 |
1981年 | 166篇 |
1980年 | 141篇 |
1979年 | 129篇 |
1978年 | 85篇 |
1977年 | 70篇 |
1976年 | 59篇 |
1973年 | 33篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
21.
22.
Amy M. LaFountain Shannon Cawley Steven J. Schwartz Richard O. Prum 《Archives of biochemistry and biophysics》2010,504(1):142-153
Recent advances in the fields of chromatography, mass spectrometry, and chemical analysis have greatly improved the efficiency with which carotenoids can be extracted and analyzed from avian plumage. Prior to these technological developments, Brush (1968) [1] concluded that the burgundy-colored plumage of the male pompadour Cotinga Xipholena punicea is produced by a combination of blue structural color and red carotenoids, including astaxanthin, canthaxanthin, isozeaxanthin, and a fourth unidentified, polar carotenoid. However, X. punicea does not in fact exhibit any structural coloration. This work aims to elucidate the carotenoid pigments of the burgundy color of X. punicea plumage using advanced analytical methodology. Feathers were collected from two burgundy male specimens and from a third aberrant orange-colored specimen. Pigments were extracted using a previously published technique (McGraw et al. (2005) [2]), separated by high-performance liquid chromatography (HPLC), and analyzed by UV/Vis absorption spectroscopy, chemical analysis, mass spectrometry, nuclear magnetic resonance (NMR), and comparison with direct synthetic products. Our investigation revealed the presence of eight ketocarotenoids, including astaxanthin and canthaxanthin as reported previously by Brush (1968) [1]. Six of the ketocarotenoids contained methoxyl groups, which is rare for naturally-occurring carotenoids and a novel finding in birds. Interestingly, the carotenoid composition was the same in both the burgundy and orange feathers, indicating that feather coloration in X. punicea is determined not only by the presence of carotenoids, but also by interactions between the bound carotenoid pigments and their protein environment in the barb rami and barbules. This paper presents the first evidence of metabolically-derived methoxy-carotenoids in birds. 相似文献
23.
The uptake of two different preparations of99mTechnetium-methylene diphosphonate in fetal rat calvaria is compared. The localization of99mTc after administration of99mTc(Sn)-MDP and99mTc-MDP showed equal distribution in autoradiography. 相似文献
24.
Isabelle Matricon 《Cell and tissue research》1973,136(4):569-578
Résumé L'épithélium pharyngien d'Alcyonidium polyoum possède des cellules pourvues d'une très grande vacuole. L'incompressibilité du liquide vacuolaire permet un élargissement brusque de l'organe lors de la contraction du manchon musculaire strié qui enserre cette vacuole. Les fibres musculaires sont insérées sur le plasmalemme apical par des filaments unitifs. Le point d'attache est relié à la lame amorphe du cell-coat qui entoure les microvillosités par des fibrilles, réalisant probablement une liaison mécamique plus efficace. Le reticulum sarcoplasmique porte des ribosomes. Le cytoplasme apical renferme des vésicules de diverses catégories.
Some ultrastructural data about a myoepithelium: The pharynx of a bryozoan
Summary Pharyngeal cells of Alcyonidium polyoum (Bryozoa) are provided with very large vacuoles. Each vacuole is enveloped by a thin layer of striated muscle, whose contraction enlarges the organ. Filaments join the muscular elements to the apical plasmalemma. This point of muscular insertion is connected by fibrils with the amorphic lamina of cell-coat which surrounds the microvilli. Ribosomes are often found on dyads. Various vesicles are located in the apical cytoplasm.相似文献
25.
Karl-D. Entian Kai-U. Fröhlich Dieter Mecke 《Biochimica et Biophysica Acta (BBA)/General Subjects》1984,799(2):181-186
An electrophoretic method has been devised to investigate the changes in the enzymes and isoenzymes of carbohydrate metabolism, upon adding glucose to derepressed yeast cell. (i) Of the glycolytic enzymes tested, enolase II, pyruvate kinase and pyruvate decarboxylase were markedly increased. This increase was accompanied by an overall increase in glycolytic activity and was prevented by cycloheximide, an inhibitor of protein synthesis. (ii) In contrast, respiratory activity decreased after adding glucose. This decrease was clearly shown to be the result of repression of respiratory enzymes. A rapid decrease within a few minutes of adding glucose, by analogy with the so-called ‘Crabtree effect’, was not observed in yeast. (iii) The gluconeogenic enzymes, fructose-1,6-bisphosphatase and malate dehydrogenase, which are inactivated after adding glucose, showed no significant changes in electrophoretic mobilities. Hence, there was no evidence of enzyme modifications, which were postulated as initiating degradation. However, it was possible to investigate cytoplasmic and mitochondrial malate dehydrogenase isoenzymes separately. Synthesis of the mitochondrial isoenzyme was repressed, whereas only cytoplasmic malate hydrogenase was subject to glucose inactivation. 相似文献
26.
27.
Brigitte Aupetit Alexandre Ghazi Nicole Blanchouin Ren e Toury Emmanuel Shechter Jean-Claude Legrand 《BBA》1988,936(3):325-331
In this study we have measured, under experimental conditions which maintained efficient coupling, respiratory intensity, respiratory control, oxidative phosphorylation capacity and protonmotive force. Succinate cytochrome-c reductase and cytochrome-c oxidase activities were also studied. These investigations were carried out using kidney mitochondria from cyclosporine-treated rats (in vivo studies) and from untreated rats in the presence of cyclosporine (in vitro studies). Inhibition of respiratory intensity by cyclosporine did not exceed 21.1% in vitro and 15.9% in vivo. Since there was no in vitro inhibition of succinate cytochrome-c reductase and cytochrome-c oxidase activities, the slowing of electron flow observed can be interpreted as a consequence of an effect produced by cyclosporine between cytochromes b and c1. Cyclosporine had no effect on respiratory control either in vitro or in vivo. Statistically significant inhibition of the oxidative phosphorylation was observed both in vitro (6.6%) and in vivo (12.1%). Moreover, cyclosporine did not induce any change of membrane potential either in vivo or in vitro. Our findings show that cyclosporine is neither a protonophore, nor a potassium ionophore. In cyclosporine-treated rats we noticed a decrease of protein in subcellular fraction, including the mitochondrial fraction. The role of the inhibition respiratory characteristics by cyclosporine in nephrotoxicity in vivo must take account of these two parameters: inhibition of the respiratory characteristics measured in vitro and diminution of mitochondrial protein in cyclosporine-treated rats. 相似文献
28.
29.
Spontaneous rupture of the Achilles tendon is increasingly common in the middle aged population. However, the cause for the particularly high incidence of injury in this age group is not well understood. Therefore, the objective of this study was to identify age-specific differences in the Achilles tendon-muscle complex using an animal model. Functional measures were performed in vivo and tissues were harvested following euthanasia for mechanical, structural, and histological analysis from young, middle aged, and old rats. Numerous alterations in tendon properties were detected across age groups, including inferior material properties (maximum stress, modulus) with increasing age. Differences in function were also observed, as older animals exhibited increased ankle joint passive stiffness and decreased propulsion force during locomotion. Macroscale differences in tendon organization were not observed, although cell density and nuclear shape did vary between age groups. Muscle fiber size and type distribution were not notably affected by age, indicating that other factors may be more responsible for age-specific Achilles tendon rupture rates. This study improves our understanding of the role of aging in Achilles tendon biomechanics and ankle function, and helps provide a potential explanation for the disparate incidence of Achilles tendon ruptures in varying age groups. 相似文献
30.