全文获取类型
收费全文 | 9736篇 |
免费 | 752篇 |
国内免费 | 544篇 |
专业分类
11032篇 |
出版年
2024年 | 20篇 |
2023年 | 126篇 |
2022年 | 151篇 |
2021年 | 280篇 |
2020年 | 286篇 |
2019年 | 317篇 |
2018年 | 270篇 |
2017年 | 275篇 |
2016年 | 299篇 |
2015年 | 317篇 |
2014年 | 446篇 |
2013年 | 602篇 |
2012年 | 403篇 |
2011年 | 375篇 |
2010年 | 351篇 |
2009年 | 403篇 |
2008年 | 437篇 |
2007年 | 419篇 |
2006年 | 439篇 |
2005年 | 396篇 |
2004年 | 367篇 |
2003年 | 395篇 |
2002年 | 361篇 |
2001年 | 317篇 |
2000年 | 325篇 |
1999年 | 254篇 |
1998年 | 248篇 |
1997年 | 206篇 |
1996年 | 222篇 |
1995年 | 177篇 |
1994年 | 169篇 |
1993年 | 161篇 |
1992年 | 128篇 |
1991年 | 122篇 |
1990年 | 108篇 |
1989年 | 102篇 |
1988年 | 92篇 |
1987年 | 82篇 |
1986年 | 70篇 |
1985年 | 75篇 |
1984年 | 87篇 |
1983年 | 48篇 |
1982年 | 59篇 |
1981年 | 51篇 |
1980年 | 43篇 |
1979年 | 51篇 |
1978年 | 36篇 |
1977年 | 19篇 |
1976年 | 15篇 |
1972年 | 8篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
131.
We studied leaf area and nitrogen dynamics in the canopy of stands of an annual herb Xanthium canadense, grown at a high (HN)- and a low-nitorgen (LN) availability. Standing leaf area increased continuously through the vegetative
growth period in the LN stand, or leveled off in the later stage in the HN stand. When scaled against standing leaf area,
both production and loss rates of leaf area increased but with different patterns: the production rate was retarded, while
the loss rate was accelerated, implying an upper limit of standing leaf area of the canopy. The rate of leaf-area production
was higher in the HN than in the LN stand, which was caused by the higher rate of leaf production per standing leaf area as
well as the greater standing leaf area in the HN stand. Although the rate of leaf-area loss was higher in the HN than in the
LN stand, it was not significantly different between the two stands when compared at a common standing leaf area, suggesting
involvement of light climate in determination of the leaf-loss rate. On the other hand, the rate of leaf-area loss was positively
correlated with nitrogen demand for leaf area development across the two stands, suggesting that leaf loss was caused by retranslocation
of nitrogen for construction of new leaves. A simple simulation model of leaf and nitrogen dynamics in the canopy showed that,
at steady state, where the rate of leaf-area loss becomes equal to the production rate, the standing leaf area was still greater
in the HN than in the LN stand. Similarly, when the uptake and loss of nitrogen are equilibrated, the standing nitrogen was
greater in the HN than in the LN stand. These results suggest that leaf-area production is strongly controlled by nitrogen
availability, while both nitrogen and light climate determine leaf-loss rates in the canopy. 相似文献
132.
West SA Shuker DM Sheldon BC 《Evolution; international journal of organic evolution》2005,59(6):1211-1228
Studies of sex allocation offer excellent opportunities for examining the constraints and limits on adaptation. A major topic of debate within this field concerns the extent to which the ability of individuals to adaptively manipulate their offspring sex ratio is determined by constraints such as the method of sex determination. We address this problem by comparing the extent of sex-ratio adjustment across taxa with different methods of sex determination, under the common selective scenario of interactions between relatives. These interactions comprise the following: local resource competition (LRC), local mate competition (LMC), and local resource enhancement (LRE). We found that: (1) species with supposedly constraining methods of sex determination showed consistent sex-ratio adjustment in the predicted direction; (2) vertebrates with chromosomal sex determination (CSD) showed less adjustment then haplodiploid invertebrates; (3) invertebrates with possibly constraining sex-determination mechanisms (CSD and pseudo-arrhenotoky) did not show less adjustment then haplodiploid invertebrates; (4) greater sex-ratio adjustment was seen in response to LRC and LMC than LRE; (5) greater sex-ratio adjustment was seen in response to interactions between relatives (LRC, LMC, and LRE) compared to responses to other environmental factors. Our results also illustrate the problem that sex-determination mechanism and selective pressure are confounded across taxa because vertebrates with CSD are influenced primarily by LRE whereas invertebrates are influenced by LRC and LMC. Overall, our analyses suggest that sex-allocation theory needs to consider simultaneously the influence of variable selection pressures and variable constraints when applying general theory to specific cases. 相似文献
133.
A set of proteins and noncoding RNAs,referred to as the male specific lethal (MSL) complex,is present on the male X chromosome in Drosophila and has been postulated to be responsible for dosage compensation of this chromosome - the up-regulation of its expression to be equal to that of two X chromosomes in females.This hypothesis is evaluated in view of lesser known aspects of dosage compensation such as the fact that metafemales with three X chromosomes also have equal expression to normal females,which would require a down-regulation of each gene copy.Moreover,when this complex is ectopically expressed in females or specifically targeted to a reporter in males,there is no increase in expression of the genes or targets with which it is associated.These observations are not consistent with the hypothesis that the MSL complex conditions dosage compensation.A synthesis is described that can account for these observations. 相似文献
134.
Norén A Lynnerup N Czarnetzki A Graw M 《American journal of physical anthropology》2005,128(2):318-323
We report on the results of applying the so-called lateral angle method for sex determination on skeletal remains. The lateral angle denotes the angle of the internal auditory canal in relation to the medial surface of the petrous part of the temporal bone. The method involves making a small cast of the proximal part of the internal acoustic canal and determining the angle at which the canal opens up to the surface of the petrous bone. The method has the great advantage of utilizing one of the sturdiest bone elements of the human skeleton, and may thus be especially suited for analyses of very fragmented skeletal remains or cremated bones, where the petrous bone may still be readily recognizable. The method was tested using a forensic sample of 113 petrous bones with known sex. Intra- and interobserver testing was also performed. We found a statistically significant difference in angle size between males and females (mean angle size of males, 39.3 degrees ; mean angle size of females, 48.2 degrees ; P < 0.001). There was no bilateral difference in angle size. In blind trials, 83.2% of petrous bones were assigned to the correct sex. We also tested the lateral angle method against an archaeological skeletal sample. True sex was not known for this sample; instead, sexing had been carried out by assessing pelvic and cranial morphology in independent trials. We found a higher concordance between the lateral angle and "pelvic" sex than for lateral angle and "cranial" sex. Finally, we note that subadult sexing may also be possible with this method. 相似文献
135.
The first evidence for female‐biased migration in a partially migratory stream‐dwelling salmonid the Dolly Varden Salvelinus malma , a phenomenon well known in sea‐run and lake‐run populations, is presented. Dolly Varden in the Shiisorapuchi River in central Hokkaido, Japan, used both tributaries, of which there are many, and the main stem, but spawned only in tributaries. The size structures of Dolly Varden (≥age 1 + years) in tributaries were unimodal (<100 mm fork length, L F ) during non‐spawning seasons but changed to bimodal during spawning seasons (lower mode <110 mm, upper mode >120 mm L F ). Mature individuals were observed in both modal groups. From the trapping and census data, the small group appeared to be tributary resident and the large group main stem migrant. Males were common in both resident and migrant components. Most females, however, migrated to the main stem to mature, indicating female‐biased migration. 相似文献
136.
Visualization of early chromosome condensation: a hierarchical folding, axial glue model of chromosome structure 总被引:10,自引:0,他引:10
Kireeva N Lakonishok M Kireev I Hirano T Belmont AS 《The Journal of cell biology》2004,166(6):775-785
Current models of mitotic chromosome structure are based largely on the examination of maximally condensed metaphase chromosomes. Here, we test these models by correlating the distribution of two scaffold components with the appearance of prophase chromosome folding intermediates. We confirm an axial distribution of topoisomerase IIalpha and the condensin subunit, structural maintenance of chromosomes 2 (SMC2), in unextracted metaphase chromosomes, with SMC2 localizing to a 150-200-nm-diameter central core. In contrast to predictions of radial loop/scaffold models, this axial distribution does not appear until late prophase, after formation of uniformly condensed middle prophase chromosomes. Instead, SMC2 associates throughout early and middle prophase chromatids, frequently forming foci over the chromosome exterior. Early prophase condensation occurs through folding of large-scale chromatin fibers into condensed masses. These resolve into linear, 200-300-nm-diameter middle prophase chromatids that double in diameter by late prophase. We propose a unified model of chromosome structure in which hierarchical levels of chromatin folding are stabilized late in mitosis by an axial "glue." 相似文献
137.
Altmann J Lynch JW Nguyen N Alberts SC Gesquiere LR 《American journal of primatology》2004,64(1):95-106
Steroid concentrations during late pregnancy and early lactation may be affected by both a female's reproductive history and her current condition, and may in turn predict subsequent life-history events, such as offspring survival. This study investigated these relationships in a wild primate population through the use of fecal steroid analysis in repeated sampling of peripartum baboons (Papio cynocephalus). Fecal samples were collected from 32 females in five groups within the Amboseli basin during 8 weeks prior to parturition and 13 weeks postpartum. From December 1999 through February 2002, 176 fecal samples were collected from individuals representing 39 peripartum periods. Fecal concentrations of progestins (fP), estrogen metabolites (fE), glucocorticoids (fGC), and testosterone metabolites (fT) were measured by radioimmunoassay. Steroid concentrations declined from late pregnancy to lactation, and the decline was greatest and most precipitous for fE and fP. Primiparous females had significantly higher mean fE concentrations in each of the last 2 months of pregnancy compared to multiparous females. Among multiparous females, fE and fT were significantly higher during late pregnancy in females carrying a male fetus compared to those carrying a female fetus. During early lactation, high fT in young mothers predicted subsequent infant death during the first year of life. These findings illustrate the potential power of repeated fecal-steroid sampling to elucidate mechanisms of life-history variability in natural populations. They also document significant differences in hormone profiles among subgroups, and highlight that such normative subgroup information is essential for interpreting individual variability in hormone-behavior associations. 相似文献
138.
A conventional balance study with 48 male weanling rats was conducted to determine true absorption and endogenous fecal excretion
of manganese (Mn) in relation to dietary Mn supply, following the procedures of a previously adapted isotope dilution technique.
After 10 d on a diet with 1.5 ppm Mn, eight animals each were assigned to diets containing 1.5, 4.5, 11.2, 35, 65, or 100
ppm Mn on a dry-matter basis. Three days later, each rat was given an intramuscular54Mn injection and kept on treatment for a balance period of 16 d.
Apparent Mn absorption assessed for the final 8 d, averaged 8.6 μg/d without significant treatment effects, although Mn intake
ranged from 18.6 to 1200 μg/d, in direct relation to dietary Mn concentrations. Mean fecal excretion of endogenous Mn for
the six treatments was 0.9, 2.7, 7.4, 11.0, 16.3, and 17.7 μg/d, respectively. These values delineate the rates to which true
absorption exceeded apparent rates. True absorption, as percent of Mn intake, averaged 28.7, 15.9, 11.7, 6.1, 3.4, and 2.0,
respectively, as compared with mean values of 23.9, 10.9, 6.2, 3.4, 1.2, and 0.5 for percent apparent absorption. It was concluded
that both true absorption and endogenous fecal excretion markedly responded to Mn nutrition and that the reduction in the
efficiency of true absorption was quantitatively the most significant homeostatic response for maintaining stable Mn concentrations
in body tissues. 相似文献
139.
Schwertz DW Vizgirda V Solaro RJ Piano MR Ryjewski C 《Molecular and cellular biochemistry》1999,200(1-2):143-153
A number of investigations in humans and animals suggest that there may be intrinsic sex-associated differences in cardiac function. Using left atrial preparations from male and female rat hearts, we examined differences in myocardial function and response to adrenergic agonists. Contractile parameters were measured in isolated atria by conventional isometric methods in the absence or presence of isoproterenol or phenylephrine. Responsiveness to Ca2+ was measured in detergent-skinned atrial fibers and actomyosin ATPase activity was measured in isolated myofibrils. Tetanic contractions were generated by treating the atrium with ryanodine followed by high frequency stimulation. Developed force was greater and maximal rates of contraction and relaxation were more rapid in the female atrium. The relationship between Ca2+ concentration and force in both intact atria and detergent-skinned atrial fibers in females fell to the left of that for males. At low Ca2+ concentrations, skinned fibers from female atria generated more force and myofibrils from female atria had higher myosin ATPase activity than males. Tetanic contraction in the presence of high extracellular Ca2+ was greater in female atria. Male atrium had larger inotropic responses to isoproterenol and to phenylephrine, but drug-elicited cAMP and inositol phosphate production did not differ between sexes. The results demonstrate sex-related differences in atrial function that can be partially explained by greater myofibrillar Ca2+-sensitivity in females. A potential contribution of sarcolemmal Ca2+ influx is suggested by greater tetanic contraction in ryanodine-treated female atrium. The larger response of males to adrenergic stimulation does not appear to be explained by higher production of relevant second messengers. Future studies will investigate the role of sex hormones in these sexually dimorphic responses and may indicate a need for gender-specific therapeutic interventions for myocardial dysfunction. 相似文献
140.
Homeobox genes, widely distributed among animal and plant kingdoms, play an important role in developmental process. Several homeobox conserved fragments were amplified by PCR and the flanking regions were also obtained by an LM-PCR procedure. Sequencing and Southern analysis showed that they belong to a homeobox gene family of rice. Six homeobox-containing fragments were mapped on the molecular linkage map of rice. They were located on chromosomes 3, 4 and 7 respectively. It is noteworthy that there are 4 homeobox fragments located on rice chromosome 3 and the result is also consistent with the comparative genomics between rice and maize. 相似文献