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51.
Lu H Wu D Jiang F Ekama GA van Loosdrecht MC Chen GH 《Biotechnology and bioengineering》2012,109(11):2778-2789
Saline water supply has been successfully practiced for toilet flushing in Hong Kong since 1950s, which saves 22% of freshwater in Hong Kong. In order to extend the benefits of saline water supply into saline sewage management, we have recently developed a novel biological organics and nitrogen removal process: the Sulfate reduction, Autotrophic denitrification, and Nitrification Integrated (SANI®) process. The key features of this novel process include elimination of oxygen demand in organic matter removal and production of minimal sludge. Following the success of a 500‐day lab‐scale trial, this study reports a pilot scale evaluation of this novel process treating 10 m3/day of 6‐mm screened saline sewage in Hong Kong. The SANI® pilot plant consisted of a sulfate reduction up‐flow sludge bed (SRUSB) reactor, an anoxic bioreactor for autotrophic denitrification and an aerobic bioreactor for nitrification. The plant was operated at a steady state for 225 days, during which the average removal efficiencies of both chemical oxygen demand (COD) and total suspended solids (TSS) at 87% and no excess sludge was purposefully withdrawn. Furthermore, a tracer test revealed 5% short circuit flow and a 34.6% dead zone in the SRUSB, indicating a good possibility to further optimize the treatment capacity of the process for full‐scale application. Compared with conventional biological nitrogen removal processes, the SANI® process reduces 90% of waste sludge, which saves 35% of the energy and reduces 36% of fossil CO2 emission. The SANI® process not only eliminates the major odor sources originating from primary treatment and subsequent sludge treatment and disposal during secondary saline sewage treatment, but also promotes saline water supply as an economic and sustainable solution for water scarcity and sewage treatment in water‐scarce coastal areas. Biotechnol. Bioeng. 2012; 109: 2778–2789. © 2012 Wiley Periodicals, Inc. 相似文献
52.
Amdiouni H Faouzi A Fariat N Hassar M Soukri A Nourlil J 《Letters in applied microbiology》2012,54(4):359-366
Aims: Reclaimed wastewater is a considerable water resource in Morocco. Its agricultural reuse requires an assessment of viral contamination. The aim of this study was to detect both infectious and noninfectious human adenoviruses (HAdV) and enteroviruses (EV) in raw wastewater and treat effluent from wastewater treatment plants (WWTPs) and domestic sewage in Morocco. Methods and Results: A total of 22 samples were analysed. A polyethylene glycol (PEG) precipitation method was used, followed by integrated cell culture‐PCR (ICC‐PCR) using two cell lines: human rhabdomyosarcoma tumour tissue and laryngeal carcinoma cells (RD and Hep2 cells). Furthermore, viral genome amplification was confirmed by sequencing. HAdV were detected in 10 (45·5%) of the 22 samples involving two species: HAdV‐B and HAdV‐D. EV was detected in 5 (23%) samples belonging to Coxsackievirus B5 and poliovirus vaccine strain (Sabin 2). Conclusions: Human adenoviruses and EV were detected in the analysed samples from two WWTPs and HAdV in domestic sewage. Significance and Impact of the Study: This work is the first study in Morocco using cell culture, PCR and sequencing of enteric viruses from wastewater. The presence of infectious HAdV and EV in treated effluent emphasizes the need of wastewater treatment surveillance. 相似文献
53.
Eva Lindvall Anne‐Maj Gustavsson Cecilia Palmborg 《Global Change Biology Bioenergy》2012,4(6):661-670
In two field experiments in northern Sweden, we investigated if intercropping reed canary grass (RCG; Phalaris arundinacea L.) with nitrogen‐fixing perennial legumes could reduce N‐fertilizer requirements and also if RCG ash or sewage sludge could be used as a supplement for mineral P and K. We compared biomass production, N uptake and N‐fixation of RCG in monoculture and mixtures of RCG with alsike clover (Trifolium hybridum L.), red clover (Trifolium pratense L.), goat's rue (Galega orientalis Lam.) and kura clover (Trifolium ambiguum M. Bieb.). In one experiment, RCG was also undersown in barley (Hordeum vulgare L.). Three fertilization treatments were applied: 100 kg N ha?1, 50 kg N ha?1 and 50 kg N ha?1 + RCG ash/sewage sludge. We used a delayed harvest method: cutting the biomass in late autumn, leaving it on the field during the winter and harvesting in spring. The legume biomass of the mixtures at the inland experimental site was small and did not affect RCG growth negatively. At the coastal site, competition from higher amount of clover biomass affected RCG growth and spring yield negatively. N‐fixation in red clover and alsike clover mixtures in the first production year approximately covered half of recommended N‐fertilization rate. Goat's rue and kura clover did not establish well at the costal site, but at the inland site goat's rue formed a small but vital undergrowth. RCG undersown in barley gave lower yield, both in autumn and spring, than the other treatments. The high N treatment gave a higher spring yield at the inland site than the low N treatments, but there were no differences due to fertilization treatments at the coastal site. For spring harvest, there were no yield benefits of RCG/legume intercropping compared with RCG monoculture. However, intercropping might be more beneficial in a two‐harvest system. 相似文献
54.
ELIZABETH E. ROSENBERGER STEPHANIE E. HAMPTON STEVEN C. FRADKIN BRIAN P. KENNEDY 《Freshwater Biology》2008,53(8):1673-1691
1. In large deep oligotrophic lakes, the shallow nearshore waters may provide the most important habitat for animals to feed and breed, and it is this area of the lake where humans are most likely to have initial impacts as the shoreline is developed. Nutrients in fertilizers, sediments and sewage effluents are likely to be rapidly intercepted by nearshore algae at the lake edge, having heterogeneous effects nearshore before offshore effects are noted. 2. Here we examined the spatially explicit effects of residential development on nearshore periphyton communities in three large deep oligotrophic lakes that have all experienced modest residential development in the Pacific Northwest of the United States. We demonstrate that substantial nearshore changes in the basal food web are detectable even with low levels of shoreline development. These changes can potentially affect whole‐lake food web dynamics. 3. For our primary study site (Lake Crescent, Washington, USA), we found that algal biomass and accumulation of detritus were higher at developed sites. In addition, both macroinvertebrate and periphyton communities exhibited a shift in composition with more detritivores and filamentous green algae at developed sites. These differences were more pronounced during the spring than at other times of year. 4. A complementary investigation of field patterns in Priest Lake and Lake Pend Oreille (Idaho, USA) suggested that, although spatial and temporal patterns were idiosyncratic, indicators of productivity and the presence of filamentous green algae were generally higher at developed sites across lakes. 5. Stable isotope signatures and water column nutrients were not useful in distinguishing developed and undeveloped sites, increasing the potential usefulness of periphyton monitoring during early stages of lake development. 6. A laboratory investigation suggested that common macroinvertebrate grazers assimilated a much greater proportion of diatoms than the filamentous green algae that are associated with fertilization at developed sites. 7. These findings have at least two clear implications: (i) periphyton may be used to detect human impacts before disturbance is evident in offshore monitoring programmes and (ii) nearshore impacts in response to modest residential development have the potential to disrupt lake food web dynamics. 相似文献
55.
Isolation of bacteria producing polyhydroxyalkanoates (PHA) from municipal sewage sludge 总被引:1,自引:0,他引:1
S. Vishnuvardhan Reddy M. Thirumala T. V. Kishore Reddy S. K. Mahmood 《World journal of microbiology & biotechnology》2008,24(12):2949-2955
Bacterial isolates from sludge samples collected at a local municipal sewage treatment plant were screened for bacteria producing
polyhydroxyalkanoates (PHA). Initially Sudan black B staining was performed to detect lipid cellular inclusions. Lipid-positive
isolates were then grown in a nitrogen limitation E2 medium containing 2% (w/v) glucose to promote accumulation of PHA before
the subsequent staining with Nile blue A. The positive isolates were quantified initially with a u.v. spectrophotometer, for
a very large number of isolates (105) and among them high PHA-producing isolates (15) were selected and were confirmed by
gas chromatographic analysis. The GC analysis showed the polymers produced by 13 of the selected isolates to be polyhydroxybutyrate
(PHB), and the remaining two isolates produced polyhydroxybutyrate-co-hydroxyvalerate (PHB-co-HV) copolymer. The proportion
of the PHA-positive bacterial isolates showed variability in the number of PHA accumulators during various months. The correlation
of PHB production with the cell dry weight (CDW) was found to be statistically significant. The metabolism of PHB in these
selected 15 isolates was studied using the Nile blue A staining, which showed an initial increase in the fluorescence followed
by a decline, on further incubation. All the selected 15 isolates were classified to genus level by studying their morphological
and biochemical characteristics. There were seven Bacillus species, three Pseudomonas species, two Alcaligenes species, two Aeromonas species, and one Chromobacterium species. 相似文献
56.
Effects of Sewage Sludge on Plant Community Composition in Restored Limestone Quarries 总被引:3,自引:0,他引:3
The effects of sewage sludge, used to improve fertility of replaced soil, on vegetation were studied in limestone quarry restoration. Plant community growing in the first stages after sludge application was surveyed in six quarries of NE Spain. Areas with a mixture of sewage sludge and residual soil were compared to areas where the application consisted only of residual soil (a mixture of previous top soil and mine spoils). Sewage sludge was hypothesized to increase total biomass and cover, modifying species composition and delaying the early successional recover of the community. The results showed that both biomass and plant cover increased because of sewage sludge addition. The floristic composition was dominated by ruderal species that did not show any dependence on sewage sludge application. Convergence on similarity between sludge and control plots was not detected along a 5‐year period. Although species richness was significantly lower in sludge plots, diversity and equitability indexes did not show differences between treatments. The results did not show differences in the proportion of non‐native species. The proportion of legumes was lower in sludge plots. These results show that the plant communities resulting from the addition of sewage sludge to the soil used in limestone quarry restoration have more biomass and cover, but less number of species, and they do not show a clear trend to converge to those areas restored only with non‐amended soil. 相似文献
57.
Wen Guang Bates T. E. Inanaga S. Voroney R. P. Hamamura K. Curtin D. 《Plant and Soil》2002,246(2):241-248
Organic wastes such as sewage sludges contain copper (Cu). Increased attention to environmental protection requires that wastes be treated with pathogen-eliminating procedures before application to farmland. It is not clear, however, if such procedures affect the plant availability of Cu in the wastes. This 2-year field research investigated the effect of irradiation and composting on Cu availability in sludges and manure using a yield control approach. Four organic wastes [digested and dewatered (DSS), digested and irradiated (DISS), composted (DICSS) sewage sludges and composted livestock manure (CLM)] were applied at four rates (10, 20, 30 and 40 t solid ha–1 year–1) with supplemented N and K fertilizers. A control treatment (CT) received N and K fertilizers only. Beans, lettuce and petunias were grown in first year and lettuce were harvested twice in second year. Beans appeared to have a strong ability to absorb Cu compared with the other test crops. In general, crop Cu concentration responses to Cu applied in DSS and DISS were well described by quadratic equations. Tested by a paired t-test, Cu concentration in bean pods was higher in DISS than in DSS treatment, indicating that irradiation increased phytoavailability of Cu. However, the parabolic response of crop Cu to Cu applied in DISS, suggesting that the increases were confined to the lower rates of DISS. Copper applied in DICSS did not increase Cu concentration in any of the test crops. At a given level of applied Cu, crop Cu tended to be lower in DICSS than in DSS or DISS treatment. Sludge composting depressed phytoavailability of Cu. Copper concentration in CLM was much lower than in sludges and sludge composts, but application of CLM increased Cu concentration in bean pods. The pattens of Cu concentration in the two cuts of lettuce in 1991 to Cu applied in CLM were similar, where low rates of CLM application slightly reduced Cu concentration, then the Cu concentration increased with increased rates. The dynamics of available Cu supply were different in the sludge composts and manure composts. 相似文献
58.
Detection and genotyping of group A rotaviruses isolated from sewage samples in Monastir,Tunisia between April 2007 and April 2010
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59.
BackgroundThe surroundings of the Cortiou sewage are among the most polluted environments of the French Mediterranean Sea (Marseilles, France). So far, no studies have precisely quantified the impact of pollution on the development of organisms in this area.MethodsWe used a fluctuating asymmetry (FA) measure of developmental instability (DI) to assess environmental stress in two species of radially symmetric sea urchins (Arbacia lixula and Paracentrotus lividus). For six sampling sites (Cortiou, Riou, Maire, East Maire, Mejean, and Niolon), levels of FA were calculated from continuous and discrete skeletal measures of ambulacral length, number of pore pairs and primary tubercles.ResultsFor both species, the most polluted sampling site, Cortiou, displayed the highest level of FA, while the Maire and East Maire sampling sites displayed the lowest levels. A. lixula revealed systematic differences in FA among sampling sites for all characters and P. lividus showed differences in FA for the number of primary tubercles.ConclusionsStatistical analyses of FA show a concordance between the spatial patterns of FA among sampling sites and the spatial distribution of sewage discharge pollutants in the Cortiou area. High developmental stress in these sampling sites is associated with exposure to high concentrations of heavy metals and many harmful organic substances contained in wastewater. FA estimated from structures with complex symmetry appears to be a fast and reliable tool to detect subtle differences in FA. Its use in biomonitoring programs for inferring anthropogenic and natural environmental stress is suggested. 相似文献
60.
The response of the marine macroalga Gracilaria edulis (Gmelin) Silva to nutrient pulses of varying magnitude was investigated to test its applicability as a marine bioindicator at two oligotrophic locations. After exposure to nutrient pulses, algal amino acid, tissue nitrogen, and chlorophyll a content were assessed relative to algae incubated under control conditions (no nutrient enrichment). The smallest nutrient pulse involved a nutrient enrichment experiment conducted within a coral atoll, whereas two larger pulses resulted from sewage discharge to a tropical coastal bay. After exposure to the smallest nutrient pulse (10 × ambient), only changes in macroalgal amino acid concentration and composition were detected (mainly as increases in citrulline). At 100 × ambient concentrations, increases in tissue % nitrogen of the macroalgae were detected, in addition to responses in amino acids. Macroalgae exposed to the highest nutrient pulse (1000 × ambient) responded with increased chlorophyll a , tissue nitrogen, and amino acids within the three day incubation period. In contrast to these algal responses, analytical water sampling techniques failed to detect elevated nutrients when nutrient pulses were not occurring. The responses of this algal bioindicator to variable nutrient pulses may provide a useful tool for investigating the source and geographical extent of nutrients entering oligotrophic coastal waters. 相似文献