全文获取类型
收费全文 | 9725篇 |
免费 | 902篇 |
国内免费 | 1656篇 |
出版年
2024年 | 24篇 |
2023年 | 187篇 |
2022年 | 186篇 |
2021年 | 278篇 |
2020年 | 370篇 |
2019年 | 422篇 |
2018年 | 348篇 |
2017年 | 368篇 |
2016年 | 387篇 |
2015年 | 384篇 |
2014年 | 410篇 |
2013年 | 685篇 |
2012年 | 459篇 |
2011年 | 459篇 |
2010年 | 397篇 |
2009年 | 499篇 |
2008年 | 438篇 |
2007年 | 550篇 |
2006年 | 460篇 |
2005年 | 481篇 |
2004年 | 410篇 |
2003年 | 390篇 |
2002年 | 295篇 |
2001年 | 332篇 |
2000年 | 288篇 |
1999年 | 229篇 |
1998年 | 242篇 |
1997年 | 235篇 |
1996年 | 201篇 |
1995年 | 197篇 |
1994年 | 179篇 |
1993年 | 184篇 |
1992年 | 156篇 |
1991年 | 151篇 |
1990年 | 121篇 |
1989年 | 88篇 |
1988年 | 71篇 |
1987年 | 89篇 |
1986年 | 86篇 |
1985年 | 101篇 |
1984年 | 109篇 |
1983年 | 50篇 |
1982年 | 65篇 |
1981年 | 52篇 |
1980年 | 47篇 |
1979年 | 31篇 |
1978年 | 18篇 |
1977年 | 17篇 |
1976年 | 20篇 |
1975年 | 12篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
891.
The exact identification of individual seed sources through genetic analysis of seed tissue of maternal origin has recently brought the full analytical potential of parentage analysis to the study of seed dispersal. No specific statistical methodology has been described so far, however, for estimation of the dispersal kernel function from categorical maternity assignment. In this study, we introduce a maximum-likelihood procedure to estimate the seed dispersal kernel from exact identification of seed sources. Using numerical simulations, we show that the proposed method, unlike other approaches, is independent of seed fecundity variation, yielding accurate estimates of the shape and range of the seed dispersal kernel under varied sampling and dispersal conditions. We also demonstrate how an obvious estimator of the dispersal kernel, the maximum-likelihood fit of the observed distribution of dispersal distances to seed traps, can be strongly biased due to the spatial arrangement of seed traps relative to source plants. Finally, we illustrate the use of the proposed method with a previously published empirical example for the animal-dispersed tree species Prunus mahaleb. 相似文献
892.
893.
Rolletschek H Nguyen TH Häusler RE Rutten T Göbel C Feussner I Radchuk R Tewes A Claus B Klukas C Linemann U Weber H Wobus U Borisjuk L 《The Plant journal : for cell and molecular biology》2007,51(3):468-484
The glucose-6-phosphate/phosphate translocator (GPT) acts as an importer of carbon into the plastid. Despite the potential importance of GPT for storage in crop seeds, its regulatory role in biosynthetic pathways that are active during seed development is poorly understood. We have isolated GPT1 from Vicia narbonensis and studied its role in seed development using a transgenic approach based on the seed-specific legumin promoter LeB4. GPT1 is highly expressed in vegetative sink tissues, flowers and young seeds. In the embryo, localized upregulation of GPT1 at the onset of storage coincides with the onset of starch accumulation. Embryos of transgenic plants expressing antisense GPT1 showed a significant reduction (up to 55%) in the specific transport rate of glucose-6-phosphate as determined using proteoliposomes prepared from embryos. Furthermore, amyloplasts developed later and were smaller in size, while the expression of genes encoding plastid-specific translocators and proteins involved in starch biosynthesis was decreased. Metabolite analysis and stable isotope labelling demonstrated that starch biosynthesis was also reduced, although storage protein biosynthesis increased. This metabolic shift was characterized by upregulation of genes related to nitrogen uptake and protein storage, morphological variation of the protein-storing vacuoles, and a crude protein content of mature seeds of transgenics that was up to 30% higher than in wild-type. These findings provide evidence that (1) the prevailing level of GPT1 abundance/activity is rate-limiting for the synthesis of starch in developing seeds, (2) GPT1 exerts a controlling function on assimilate partitioning into storage protein, and (3) GPT1 is essential for the differentiation of embryonic plastids and seed maturation. 相似文献
894.
895.
896.
A protein phosphatase 2A catalytic subunit is a negative regulator of abscisic acid signalling 总被引:1,自引:0,他引:1
Pernas M García-Casado G Rojo E Solano R Sánchez-Serrano JJ 《The Plant journal : for cell and molecular biology》2007,51(5):763-778
The key regulatory role of abscisic acid (ABA) in many physiological processes in plants is well established. However, compared with other plant hormones, the molecular mechanisms underlying ABA signalling are poorly characterized. In this work, a specific catalytic subunit of protein phosphatase 2A (PP2Ac-2) has been identified as a component of the signalling pathway that represses responses to ABA. A loss-of-function pp2ac-2 mutant is hypersensitive to ABA. Moreover, pp2ac-2 plants have altered responses in developmental and environmental processes that are mediated by ABA, such as primary and lateral root development, seed germination and responses to drought and high salt and sugar stresses. Conversely, transgenic plants overexpressing PP2Ac-2 are less sensitive to ABA than wild type, a phenotype that is manifested in all the above-mentioned physiological processes. DNA microarray hybridization experiments reveal that PP2Ac-2 is negatively involved in ABA responses through regulation of ABA-dependent gene expression. Moreover, the results obtained indicate that ABA antagonistically regulates PP2Ac-2 expression and PP2Ac-2 activity thus allowing plant sensitivity to the hormone to be reset after induction. Phenotypic, genetic and gene expression data strongly suggest that PP2Ac-2 is a negative regulator of the ABA pathway. Activity of protein phosphatase 2A thus emerges as a key element in the control of ABA signalling. 相似文献
897.
本文应用响应面分析的方法研究了酶用量、酶解温度和pH对薏苡仁油提取率的影响,并得到了最佳的提取条件。研究结果表明,酶用量为1.569%、酶解温度在47.7℃、酶解pH为4.75。响应的薏苡仁油提取率为10.948%。油脂质量分析结果表明,酶法处理并没有显著改变薏苡仁油的性质。 相似文献
898.
Wim A. Ozinga Stephan M. Hennekens Joop H.J. Schamine Nina A.C. Smits Rene M. Bekker Christine Rmermann Leo Klime Jan P. Bakker Jan M. van Groenendael 《植被学杂志》2007,18(4):489-497
Questions: 1. Which plant traits and habitat characteristics best explain local above‐ground persistence of vascular plant species and 2. Is there a trade‐off between local above‐ground persistence and the ability for seed dispersal and below‐ground persistence in the soil seed bank? Locations: 845 long‐term permanent plots in terrestrial habitats across the Netherlands. Methods: We analysed the local above‐ground persistence of vascular plants in permanent plots (monitored once a year for ca. 16 year) with respect to functional traits and habitat preferences using survival statistics (Kaplan‐Meier analysis and Cox’ regression). These methods account for censored data and are rarely used in vegetation ecology. Results: Local above‐ground persistence is determined by both functional traits (especially the ability to form long‐lived clonal connections) and habitat preferences (especially nutrient requirements). Above‐ground persistence is negatively related to the ability for dispersal by wind and to the ability to accumulate a long‐term persistent soil seed bank (‘dispersal through time’) and is positively related to the ability for dispersal by water. Conclusions: Most species have a half‐life expectation over 15 years, which may contribute to time lags after changes in habitat quality or ‐configuration (‘extinction debt’). There is evidence for a trade‐off relationship between local above‐ground persistence and below‐ground seed persistence, while the relationship with dispersal in space is vector specific. The rate of species turnover increases with productivity. 相似文献
899.
Questions: What is the accuracy and reliability of the commonly used random soil sampling methodology for predicting seedling density, species richness and composition of the emerging seedling community? Location: Lake Kraenepoel, western Belgium. Methods: We compared density, species composition and observed and rarefactioned species richness of the seedling community emerging on a soft water lake bed exposed after drainage with the seedling community germinating in the laboratory from random soil samples in the same plots. Results: Seedling density did not differ between the two methods and there was a significant correlation between seedling density on the exposed lake bed and in the soil samples. This indicates that future seedling density can be reliably predicted based on soil sampling, in particular for the most abundant species. The most frequently occurring and abundant species among the seedlings in the soil samples were also the most frequent and abundant species germinating on the exposed lake bed. In contrast, species richness was much higher on the exposed lake bed than in the soil samples, and this difference was still significant for annual species after correction for differences in sampling intensity by rarefaction. We found no correlation between the number of species retrieved by the two methods. Although seedlings of rare and target species emerged on the lake bed, random soil sampling clearly failed to detect seeds of most of these species. Conclusions: Random soil sampling at a commonly used intensity and using the standard germination conditions can accurately predict future total seedling density and the density of the most abundant species. However, the method is not reliable for predicting the probability of establishment of populations of uncommon species. When executing a seed bank study, sampling intensity and germination conditions need to be adapted to the nature and the level of detail of the research question to be answered. 相似文献
900.
Omotayo Sarafadeen Amuda Olumuyiwa Idowu Ojo Theresa Ibibia Edewor 《Bioremediation Journal》2007,11(4):183-194
The shell of the seed of Chrysophyllum albidum carbon was used to adsorb lead (Pb) from aqueous solution, the sorption process with respect to its equilibria and kinetics as well as the effects of pH, contact time, adsorbent mass, adsorbate concentration, and particle size on adsorption were also studied. The most effective pH range was found to be between 4.5 and 5 for the sorption of the metal ion. The first-order rate equation by Lagergren was tested on the kinetic data and the adsorption process followed first-order rate kinetics. Isotherm data were analyzed for possible agreement with the Langmuir and Freundlich adsorption isotherms; the Freundlich and Langmuir models for dynamics of metal ion uptake proposed in this work fitted the experimental data reasonably well. However, equilibrium sorption data were better represented by Langmuir model than Freundlich. The adsorption capacity calculated from Langmuir isotherm was 72.1 mg Pb (II) g- 1 at initial pH of 5.0 at 30°C for the particle size of 1.00 to 1.25 mm with the use of 2.0 g/100 ml adsorbent mass. The structural features of the adsorbent were characterized by Fourier transform infrared (FTIR) spectrometry; the presence of hydroxyl, carbonyl, amide, and phosphate groups confirms the potential mechanism adsorption of the adsorbent. This readily available adsorbent is efficient in the uptake of Pb (II) ion in aqueous solution, thus, it could be an excellent alternative for the removal of heavy metals and organic matter from water and wastewater. 相似文献