全文获取类型
收费全文 | 6520篇 |
免费 | 602篇 |
国内免费 | 554篇 |
出版年
2024年 | 13篇 |
2023年 | 121篇 |
2022年 | 98篇 |
2021年 | 180篇 |
2020年 | 244篇 |
2019年 | 281篇 |
2018年 | 206篇 |
2017年 | 212篇 |
2016年 | 215篇 |
2015年 | 217篇 |
2014年 | 229篇 |
2013年 | 418篇 |
2012年 | 285篇 |
2011年 | 270篇 |
2010年 | 245篇 |
2009年 | 306篇 |
2008年 | 273篇 |
2007年 | 346篇 |
2006年 | 302篇 |
2005年 | 317篇 |
2004年 | 268篇 |
2003年 | 259篇 |
2002年 | 186篇 |
2001年 | 201篇 |
2000年 | 185篇 |
1999年 | 158篇 |
1998年 | 178篇 |
1997年 | 154篇 |
1996年 | 135篇 |
1995年 | 130篇 |
1994年 | 110篇 |
1993年 | 110篇 |
1992年 | 104篇 |
1991年 | 94篇 |
1990年 | 71篇 |
1989年 | 66篇 |
1988年 | 52篇 |
1987年 | 47篇 |
1986年 | 48篇 |
1985年 | 79篇 |
1984年 | 72篇 |
1983年 | 32篇 |
1982年 | 35篇 |
1981年 | 32篇 |
1980年 | 24篇 |
1979年 | 16篇 |
1978年 | 10篇 |
1977年 | 8篇 |
1976年 | 14篇 |
1973年 | 6篇 |
排序方式: 共有7676条查询结果,搜索用时 296 毫秒
191.
192.
The concept of biological control methods in aquaculture 总被引:10,自引:0,他引:10
Masachika Maeda Kinya Nogami Masaei Kanematsu Kazutsugu Hirayama 《Hydrobiologia》1997,358(1-3):285-290
Microbial techniques of biocontrol using the interaction ofmicroorganisms to repress the growth of deleterious bacteria andviruses were developed. The bacterial strain used in this work alsoimproved the growth of fishes and crustaceans. Using the conceptandprocedures of the biocontrol method described here, the aquacultureproduction became stable and evenincreased. 相似文献
193.
Development of black dot disease (Colletotrichum coccodes (Wallr.) Hughes) and its effects on the growth and yield of potato plants 总被引:1,自引:0,他引:1
Seed tubers of cvs Désirée and Pentland Crown with different severities of black dot were planted in 1988 and 1989 at Rothamsted in fields in 4– or 7-course rotations, respectively. Tubers treated with prochloraz (1988) or imazalil (1989) were planted in some plots, and in others Colletotrichum coccodes inoculum was added to the soil at planting. In further experiments at Mepal, Cambridgeshire in 1989 and 1990 and at Rothamsted in 1990 on sites where potatoes had not been grown for more than 15 years, large amounts of inoculum were added to the soil around disease-free seed tubers of two (1989) or three (1990) cultivars at planting. In all experiments plants were sampled during the season and the effects of treatments on disease development, growth and yield were recorded. Disease on roots, stem bases and tubers was found early in the season and was more severe on Désirée than on Pentland Crown plants from fields in 4– or 7-course rotations. Severity increased throughout the season and with increasing amounts of disease on the seed tubers, especially with Desiree. Disease was also found on plants from disease-free tubers and was more severe in 1988 than 1989. At harvest, black dot on tubers was significantly more severe from severely affected than from disease-free seed, and was most severe where inoculum, especially large amounts, had been added at planting. Fungicide treatment decreased disease early in the season but had no effect on tuber infection at harvest. In 1989 the weight loss of seed tubers during sprouting increased with increasing amounts of black dot, but the disease had little effect on plant size through the season. At harvest the yield of ware tubers (>50 mm) decreased with severe disease but total tuber yields were not significantly affected. However, at harvest in 1988 severely affected seed yielded significantly less than healthy seed. Plants grown from mini-tubers were free from disease on sites where potatoes had not been grown for at least 15 years. Inoculum applied at planting caused severe disease on all cultivars in both years, whereas disease was slight on uninoculated plants. Inoculated plants senesced early at Mepal in 1990, but there were no significant differences in total tuber yield in any experiment. However, yields of ware tubers (>50 mm) were sometimes decreased and the total tuber number per plant increased. 相似文献
194.
The effects of different seed sizes, planting densities and planting patterns on the transmission of silver scurf (Helminthosporium solani) and black scurf (Rhizoctonia solani) diseases of potatoes were examined in five field experiments with cv. Estima in 1991–93.
In all experiments, silver scurf was more severe with increase in seed size and planting density. At high planting density, silver scurf was less severe from a square planting pattern than from planting in wide rows. The incidence of black scurf also tended to increase with increase in seed size and was increased markedly high planting density. 相似文献
In all experiments, silver scurf was more severe with increase in seed size and planting density. At high planting density, silver scurf was less severe from a square planting pattern than from planting in wide rows. The incidence of black scurf also tended to increase with increase in seed size and was increased markedly high planting density. 相似文献
195.
Seeds of cauliflower cv. Hipop and Brussels sprouts cv. Asmer Aries were aged at 20% moisture content for 24 h; all seeds retained a germination of over 70% after ageing although the mean germination time increased. Prolonged aerated hydration for up to 32 h at 20°C followed drying resulted in improved performance of both unaged and aged cauliflower seeds and aged Brussels sprouts. Thus, all seed showed reductions in the mean germination time to the extent that after 32 h hydration the aged cauliflower seeds performed as well as high quality unaged seed. The improvement of aged seeds was also revealed an increase in germination after the controlled deterioration test following up to 24 h (cauliflower) or 32 h (Brussels sprouts) aerated hydration. This increase was indicative of a decrease in the extent of deterioration present after aerated hydration. Deleterious effects of prolonged hydration were observed in Brussels sprouts after 32 h although these may be explained desiccation injury after treatment since radicle emergence had occurred during hydration. The improvements in seed performance may be explained the activation of metabolic repair occurring during the early part of the hydration period therereducing the extent of deterioration that has been sustained during ageing, with further improvements due to the advancement of the germination process. 相似文献
196.
赤霉素与脱落酸对番茄种子萌发中细胞周期的调控 总被引:11,自引:0,他引:11
利用细胞流检仪检测番茄(Lycopersicon esculentum Mill.) GA-缺陷型、ABA-缺陷型和相应的正常品种(野生型)成熟种子胚根尖细胞倍性水平时发现:GA-缺陷型和野生型种子绝大多数细胞DNA 水平为2C,而ABA-缺陷型种子则含有较多的4C细胞。在标准发芽条件下,ABA-缺陷型和野生型种子浸种1 d 后胚根尖细胞DNA 开始复制,随后胚根突破种皮而发芽。然而GA-缺陷型种子除非加入外源GA,否则既不发生细胞DNA 复制,也不发芽。这说明内源GA 是启动番茄种子胚根尖细胞DNA 复制的关键因素,同时也说明番茄根尖细胞DNA 复制是种子发芽的必要条件。实验证明:ABA 不抑制细胞DNA 合成,但阻止G2 细胞进入到M 期。外源ABA处理野生型种子与渗控处理结果相似,可以大幅度提高胚根尖4C/2C细胞的比例,但抑制种子的最终发芽 相似文献
197.
沙坡头人工固沙区的种子库动态 总被引:77,自引:0,他引:77
沙坡头人工固沙区的土壤种子库中共有46种植物的种子,属于17个科。其中菊科植物最多,达11种;其次为藜科,7种;禾本科7种;豆科4种。既是现有植被组分种,其种子又存在于种子库中的,占总种数的33.3% 。油蒿(Artem isia ordosica)、花棒(Hedysarum scoparium )和柠条(Caragana korshinskii)的种子产量为545±676.73粒/m 2、6.67±13.74粒/m 2、0.032±2.43×10- 3粒/m 2。油蒿在植株上成熟时不受虫害,而花棒、柠条的种子受害比例分别为31.92% 和83.29% 。在最适埋藏深度下,油蒿、柠条、花棒种子的累积发芽率分别可达78.3% 、85.0% 和66.7% ;萌发速度:油蒿> 柠条> 花棒。根据种子库动态分析和植被调查结果可初步预测:沙坡头人工固沙植被的演替趋向将是灌木以油蒿为主,草本以雾冰藜(Bassia dasyphylla)、小画眉草(Eragrostispoaeoides)为主的群落 相似文献
198.
干燥番茄(Lycopersicon esculentum Mill. cv. Moneym aker)种子浸种吸水有明显的3 个阶段,尽管种子不同部分吸水的速度不一样,但整粒种子需10~12 h 才能基本完成第一阶段的吸水。胚乳对种胚伸长和生长的机械压力明显阻碍种胚的吸水,即使在第二阶段整个种子水势与浸种溶液基本达到水分平衡时,种胚的水势仍然低于整个种子水势0.6~0.9 MPa。发芽过程中GA 和ABA不直接影响番茄种子吸水。番茄种胚水势与含水量的关系呈幂指数的正相关。无论在浸种还是渗控处理过程中,番茄种胚压力势的总变化趋势是下降的 相似文献
199.
Cassettes for seed-specific expression tested in transformed embryogenic cultures of soybean 总被引:4,自引:0,他引:4
Soybean (Glycine max [L.] Merrill) lectin is a seed protein that accumulates in protein bodies of cotyledons during seed development. We have
constructed two expression cassettes containing the 5′ and 3′ regions of the soybean lectin gene connected by aNot I restriction site. One vector also contains the 32 amino acid signal sequence. Using polymerase chain reaction (PCR), the
coding region of the β-glucuronidase (uidA) gene was inserted into theNot I site of each vector. We tested the function of the expression cassettes in transformed embryogenic cultures of soybean.
Development-specific GUS expression was observed in developing somatic embryos transformed with the chimeric lectin promoter-GUS
constructs as determined by histochemical assays. Our data indicate that these cassettes could be used to drive expression
of foreign genes to modify embryo-specific traits of soybean as protein quality or quantity in the seed. 相似文献
200.