全文获取类型
收费全文 | 35482篇 |
免费 | 2262篇 |
国内免费 | 1467篇 |
专业分类
39211篇 |
出版年
2024年 | 94篇 |
2023年 | 600篇 |
2022年 | 803篇 |
2021年 | 1014篇 |
2020年 | 1101篇 |
2019年 | 1476篇 |
2018年 | 1288篇 |
2017年 | 935篇 |
2016年 | 960篇 |
2015年 | 1022篇 |
2014年 | 1845篇 |
2013年 | 2303篇 |
2012年 | 1420篇 |
2011年 | 1830篇 |
2010年 | 2133篇 |
2009年 | 1591篇 |
2008年 | 1579篇 |
2007年 | 1762篇 |
2006年 | 1533篇 |
2005年 | 1563篇 |
2004年 | 1555篇 |
2003年 | 1231篇 |
2002年 | 939篇 |
2001年 | 760篇 |
2000年 | 588篇 |
1999年 | 655篇 |
1998年 | 563篇 |
1997年 | 515篇 |
1996年 | 522篇 |
1995年 | 547篇 |
1994年 | 513篇 |
1993年 | 477篇 |
1992年 | 440篇 |
1991年 | 386篇 |
1990年 | 309篇 |
1989年 | 291篇 |
1988年 | 280篇 |
1987年 | 218篇 |
1986年 | 225篇 |
1985年 | 197篇 |
1984年 | 214篇 |
1983年 | 109篇 |
1982年 | 175篇 |
1981年 | 147篇 |
1980年 | 132篇 |
1979年 | 92篇 |
1978年 | 71篇 |
1977年 | 66篇 |
1976年 | 58篇 |
1972年 | 23篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
102.
Akiko Nishimura 《Molecular & general genetics : MGG》1989,215(2):286-293
Summary A novel mutant of Escherichia coli, named cfcA1, was isolated from a temperature-sensitive dnaB42 strain, and found to have the following characteristics. Division arrest and lethality induced by inhibition of DNA replication was reduced and delayed in the cfcA1 dnaB42 strain, as compared with the parental dnaB42 strain. Two types of inhibition of division induced by the addition of nalidixic acid or hydroxyurea were suppressed by the cfcA1 mutation. Under permissive conditions for DNA replication, the colony forming ability of cfcA1 cells was significantly reduced as compared with that of cfc
+ cells; conversely the division rate of cfcA1 cells was higher than that of cfc
+ cells. The cfcA1 mutation partially restored division arrest induced in the thermosensitive ftsZ84 mutant at the restrictive temperature and suppresed the UV sensitivity of the lon mutation. The mutation was mapped at 79.2 min on the E. coli chromosome. Taking these properties into account, it is hypothesized that the cfcA gene is involved in determining the frequency of cell division per round of DNA replication by interacting with the FtsZ protein which is essential for cell division. 相似文献
103.
Summary M1 is a virulent bacteriophage of Methanobacterium thermoautotrophicum strain Marburg. Restriction enzyme analysis of the linear, 30.4 kb phage DNA led to a circular map of the 27.1 kb M1 genome. M1 is thus circularly permuted and exhibits terminal redundancy of approximately 3 kb. Packaging of M1 DNA from a concatemeric precursor initiates at the pac site which was identified at coordinate 4.6 kb on the circular genome map. It proceeds clockwise for at least five packaging rounds. Headful packaging was also shown for M2, a phage variant with a 0.7 kb deletion at coordinate 23.25 on the map. 相似文献
104.
105.
Matilde Jose Isabelle Tratner Maryse Poiret Jean-Louis Nahon Jean-Louis Danan Jose Maria Sala-Trepat 《Molecular & general genetics : MGG》1989,215(2):225-230
Summary The distribution of middle repetitive sequences in the genic and extragenic regions of the rat albumin and -fetoprotein genes was analyzed. Their presence was determined by probing Southern blots of restriction fragments of albumin and -fetoprotein genomic subclones with 32P-labeled total rat DNA. Repetitive sequences were detected in both genes. They were classified as weak, moderate and intense hybridizing elements according to the intensity of hybridization. Weak repetitive sequences were characterized as dG·dT repeats by using 32P-labeled poly-(dG·dT)(dC·dA) oligomer probe. They occurred in 5 and 3 extragenic regions of the two genes and in introns 4 and 5 of the albumin gene. The moderate repetitive sequence present in intron 6 of the albumin gene was identified as the rat SINES element, 4D12. The intense repetitive sequence, localized in the 3 non-coding region of the albumin gene, corresponded to the terminal segment of a rat high repeat long interspersed DNA family, L1Rn. 4D12 and L1Rn sequences were also scattered throughout the -fetoprotein locus as moderate and intense repetitive elements, respectively, but their distribution was different from that of the albumin genomic region. These results indicate that repetitive sequences invaded the two loci in a non-conservative manner. 相似文献
106.
107.
108.
Summary Hamilton and Zuk (1982) proposed that the quality of male showy traits reflects genetically-based resistance to parasites and can be used by females to select mates that are less prone to parasitic attack. The hypothesis requires that a particular state of a variable showy trait should be associated with parasite infection. We tested this idea with a population of western fence lizards, Sceloporus occidentalis, infected with the malarial parasite, Plasmodium mexicanum. Ventral color pattern is strongly dimorphic in fence lizards and varies greatly among males in this population. Malaria-infected males exhibited significantly more black and less pale on their ventral surface than did noninfected males of similar body size. This difference was not a function of differing ages of infected and noninfected animals of the same body size. However, logistic regression demonstrated that females using male ventral color as a gauge of infection status would only marginally improve their chance of choosing a noninfected lizard over random selection of mates. 相似文献
109.
110.
Tomato (Lycopersicon esculentum Mill. cv. Moneymaker) plants have been wounded to induce the accumulation of proteinase-inhibitor proteins (PI proteins) at the local site of injury and systemically in unwounded tissues. To determine the range of genes affected in the wound-response, polysomal mRNA has been isolated from the damaged leaves and from systemically responding leaves over a time-course of 2, 4, 10 and 24 h after wounding. Changes in the pattern of 35S-translation products indicate that the events that occur at the local wound-site are different from those that occur systemically, both with respect to the number of genes that are regulated and the timing of their regulation. In order to compare the effects of wounding and an endogenous systemic signal generated at the wound-site with those of elicitor (proteinase-inhibitor-inducing factor, PIIF) treatment of excised plants, polysomal mRNA has also been isolated from leaves of plants over a time-course of 2, 4, 10 and 24 h after PIIF-treatment. Changes in the pattern of 35S-translation products indicates that the events induced by PIIF resemble those induced by mechanical injury, rather than those induced by the endogenous systemic signal.Abbreviations IFF
isoelectric focussing
- PI proteins
proteinase inhibitor proteins
- PIIF
proteinase-inhibitor-inducing factor
- ssRubisco
small subunit of ribulose-1,5-bisphosphate carboxylase 相似文献