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201.
Response of small birch plants (Betula pendula Roth.) to elevated CO2 and nitrogen supply 总被引:5,自引:1,他引:4
Small birch plants were grown for up to 80 d in a climate chamber at varied relative addition rates of nitrogen in culture solution, and at ambient (350 μmol mol-1) or elevated (700 μmol mol-1) concentrations of CO2. The relative addition rate of nitrogen controlled relative growth rate accurately and independently of CO2 concentration at sub-optimum levels. During free access to nutrients, relative growth rate was higher at elevated CO2. Higher values of relative growth rate and net assimilation rate were associated with higher values of plant N-concentration. At all N-supply rates, elevated CO2 resulted in higher values of net assimilation rate, whereas leaf weight ratio was independent of CO2. Specific leaf area (and leaf area ratio) was less at higher CO2 and at lower rates of N-supply. Lower values of specific leaf area were partly because of starch accumulation. Nitrogen productivity (growth rate per unit plant nitrogen) was higher at elevated CO2. At sub-optimal N-supply, the higher net assimilation rate at elevated CO2 was offset by a lower leaf area ratio. Carbon dioxide did not affect root/shoot ratio, but a higher fraction of plant dry weight was found in roots at lower N-supply. In the treatment with lowest N-supply, five times as much root length was produced per amount of plant nitrogen in comparison with optimum plants. The specific fine root length at all N-supplies was greater at elevated CO2. These responses of the root system to lower N-supply and elevated CO2 may have a considerable bearing on the acquisition of nutrients in depleted soils at elevated CO2. The advantage of maintaining steady-state nutrition in small plants while investigating the effects of elevated CO2 on growth is emphasized. 相似文献
202.
L. Schreiber H.-W. Breiner M. Riederer M. Düggelin R. Guggenheim 《Plant biology (Stuttgart, Germany)》1994,107(5):353-361
The root endodermis of Clivia miniata Reg. was successfully isolated using the cell wall degrading enzymes cellulase and pectinase. The enzymes did not depolymerize those regions of the primary cell walls of anticlinal endodermal root cells where the Casparian strips were located. Since the endodermis of C. miniata roots remained in its primary developmental state over the whole root length, endodermal isolates essentially represented Casparian strips. Thus, sufficient amounts of isolated Casparian strips could be obtained to allow further detailed investigations of the isolates by microscopic, histochemical and analytical methods. Scanning electron microscopy revealed the reticular structure of the Casparian strips completely surrounding the central cylinder of the roots. Whereas in younger parts of the root only the anticlinal cell walls of the endodermis remained intact in the isolates, in older parts of the root the periclinal walls also restricted enzymatic degradation due to the deposition of lignin. Extracts of the isolates with organic solvents did not reveal any wax-like substances which might have been deposited within the cell wall forming a transport barrier, as is the case with cutin and suberin. However, several histochemical and analytical methods (elemental analysis and FTIR spectroscopy) showed that the chemical nature of the Casparian strips of C. miniata roots can definitely be a lignified cell wall. These findings are in complete agreement with studies carried out at the beginning of this century on the chemical nature of the Casparian strips of several other plant species. The implications of these results concerning apoplasmatic transport of solutes and water across Casparian strips are discussed. 相似文献
203.
204.
The characteristics of sulphate uptake into right-side-out plasma-membrane vesicles isolated from roots of Brassica napus L., Metzger, cv. Drakkar, and purified by aqueous polymer two-phase partitioning, were investigated. Sulphate uptake into the vesicles was driven by an artificially imposed pH gradient (acid outside), and could be observed for 5–10 min before a plateau was reached and no further net uptake occurred. The uptake was partially inhibited in the presence of depolarizing agents and little uptake was observed in the absence of an imposed pH gradient. Uptake was strongly pH-dependent, being greatest at more acidic pH. After imposition of a pH gradient, the capacity for uptake decreased slowly (t1/2>10 min). The uptake had a high-affinity component which was strongly dependent on the external proton concentration (K m=10μM at pH 5.0, 64 μM at pH 6.5). The K m for protons varied from 0.4–1.9 μM as the sulphate concentration was reduced from 33 to 1 μM. A low-affinity component was observed which could be resolved at low temperatures (0 °C). Microsomal membranes that partitioned into the lower phase of the two-phase system gave no indication of high-affinity sulphate transport. Sulphate uptake into plasma-membrane vesicles isolated from sulphur-starved plant material was approximately twofold greater than that observed in those isolated from sulphate-fed plant material. Isolated vesicles therefore mirror the well-known in-vivo response of roots, indicating an increase in the number of transporters to be, at least in part, the underlying cause of derepression. 相似文献
205.
The presence of the glycolytic enzymes from hexokinase to pyruvate kinase in plastids of seedling pea (Pisum sativum L.) roots was investigated. The recoveries, latencies and specific activities of each enzyme in different fractions was compared with those of organelle marker enzymes. Tryptic-digestion experiments were performed on each enzyme to determine whether activities were bound within membranes. The results indicate that hexokinase (EC 2.7.1.2) and phosphoglyceromutase (EC 5.4.2.1) are absent from pea root plastids. The possible function of the remaining enzymes is considered.Abbreviations GADPH
glyceraldehyde 3-phosphate dehydrogenase
- PFK
phosphofructokinase
- PFP
pyrophosphate: fructose 6-phosphate 1-phosphotransferase
Bronwen A. Trimming gratefully acknowledges the award of a studentship from the Science and Engineering Research Council 相似文献
206.
Synthesis of indole-3-acetic acid (IAA), using stable-isotope incorporation, was investigated in Zea mays L. Incorporation of 2H from 2H2O into IAA molecules was shown to occur in intact plantlets and excised primary roots cultured in vitro. This demonstrates the de-novo formation of IAA, a process which is quantitatively well defined and is initiated early in germination.Abbreviations IAA
indole-3-acetic acid 相似文献
207.
The behavior of cell nuclei, mitochondrial nucleoids (mt-nucleoids) and plastid nucleoids (ptnucleoids) was studied in the
root apical meristem of Arabidopsis thaliana. Samples were embedded in Technovit 7100 resin, cut into thin sections and stained with 4′-6-diamidino-2-phenylindole for
light-microscopic autoradiography and microphotometry. Synthesis of cell nuclear DNA and cell division were both active in
the root apical meristem between 0 μm and 300 μm from the central cells. It is estimated that the cells generated in the lower
part of the root apical meristem enter the elongation zone after at least four divisions. Throughout the entire meristematic
zone, individual cells had mitochondria which contained 1–5 mt-nucleoids. The number of mitochondria increased gradually from
65 to 200 in the meristem of the central cylinder. Therefore, throughout the meristem, individual mitochondria divided either
once or twice per mitotic cycle. By contrast, based on the incorporation of [3H]thymidine into organelle nucleoids, syntheses of mitochondrial DNA (mtDNA) and plastid DNA (ptDNA) occurred independently
of the mitotic cycle and mainly in a restricted region (i.e., the lower part of the root apical meristem). Fluorimetry, using
a videointensified microscope photon-counting system, revealed that the amount of mtDNA per mt-nucleoid in the cells in the
lower part of the meristem, where mtDNA synthesis was active, corresponded to more than 1 Mbp. By contrast, in the meristematic
cells just below the elongation zone of the root tip, the amount of mtDNA per mt-nucleoid fell to approximately 170 kbp. These
findings strongly indicate that the amount of mtDNA per mitochondrion, which has been synthesized in the lower part of the
meristem, is gradually reduced as a result of continual mitochondrial divisions during low levels of mtDNA synthesis. This
phenomenon would explain why differentiated cells in the elongation zone have mitochondria that contain only extremely small
amounts of mtDNA.
This work was supported by a Grant-in Aid (T.K.) for Special Research on Priority Areas (Project No. 02242102, Cellular and
Molecular Basis for Reproduction Processes in Plants) from the Ministry of Education, Science and Culture of Japan and by
a Grant-in Aid (T.K.) for Original and Creative Research Project on Biotechnology from the Research Council, Ministry of Agriculture,
Forestry and Fisheries of Japan. 相似文献
208.
Johann Sebastian Braun Brigitte Kaissling Michel Le Hir Wolfgang Zenker 《Cell and tissue research》1993,273(2):209-217
This report deals with the distribution, morphology and specific topical relationships of bone-marrow-derived cells (free cells) in the spinal meninges and dorsal root ganglia of the normal rat. The morphology of these cells has been studied by transmission and scanning electron microscopy. Cells expressing the major histocompatibility complex (MHC) class II gene product have been recognized by immunofluorescence. At the level of the transmission electron microscope, free cells are found in all layers of the meninges. Many of them display characteristic ultrastructural features of macrophages, whereas others show a highly vacuolated cytoplasm and are endowed with many processes. These elements lack a conspicuous lysosomal system and might represent dendritic cells. Scanning electron microscopy has revealed that free cells contact the cerebrospinal fluid via abundant cytoplasmic processes that cross the cell layers of the pia mater and of the arachnoid. Cells expressing the MHC class II antigen are also found in all layers of the meninges. They are particularly abundant in the layers immediately adjacent to the subarachnoid space, in the neighbourhood of dural vessels, along the spinal roots and in the dural funnels. In addition to the meninges, strong immunoreactivity for MHC class II antigen is observed in the dorsal root ganglia. The ultrastructural and immunohistochemical findings of this study suggest the existence of a well-developed system of immunological surveillance of the subarachnoid space and of the dorsal root ganglia. 相似文献
209.
Marie Arnaud Stefan Krause Richard J. Norby Thuong Huyen Dang Nezha Acil Nicholas Kettridge Vincent Gauci Sami Ullah 《Global Change Biology》2023,29(12):3256-3270
Mangroves are among the most carbon-dense ecosystems worldwide. Most of the carbon in mangroves is found belowground, and root production might be an important control of carbon accumulation, but has been rarely quantified and understood at the global scale. Here, we determined the global mangrove root production rate and its controls using a systematic review and a recently formalised, spatially explicit mangrove typology framework based on geomorphological settings. We found that global mangrove root production averaged ~770 ± 202 g of dry biomass m−2 year−1 globally, which is much higher than previously reported and close to the root production of the most productive tropical forests. Geomorphological settings exerted marked control over root production together with air temperature and precipitation (r2 ≈ 30%, p < .001). Our review shows that individual global changes (e.g. warming, eutrophication, drought) have antagonist effects on root production, but they have rarely been studied in combination. Based on this newly established root production rate, root-derived carbon might account for most of the total carbon buried in mangroves, and 19 Tg C lost in mangroves each year (e.g. as CO2). Inclusion of root production measurements in understudied geomorphological settings (i.e. deltas), regions (Indonesia, South America and Africa) and soil depth (>40 cm), as well as the creation of a mangrove root trait database will push forward our understanding of the global mangrove carbon cycle for now and the future. Overall, this review presents a comprehensive analysis of root production in mangroves, and highlights the central role of root production in the global mangrove carbon budget. 相似文献
210.
Simona Picardi Briana Abrahms Emily Gelzer Thomas A. Morrison Tana Verzuh Jerod A. Merkle 《Ecology letters》2023,26(1):157-169
Site fidelity—the tendency to return to previously visited locations—is widespread across taxa. Returns may be driven by several mechanisms, including memory, habitat selection, or chance; however, pattern-based definitions group different generating mechanisms under the same label of ‘site fidelity’, often assuming memory as the main driver. We propose an operational definition of site fidelity as patterns of return that deviate from a null expectation derived from a memory-free movement model. First, using agent-based simulations, we show that without memory, intrinsic movement characteristics and extrinsic landscape characteristics are key determinants of return patterns and that even random movements may generate substantial probabilities of return. Second, we illustrate how to implement our framework empirically to establish ecologically meaningful, system-specific null expectations for site fidelity. Our approach provides a conceptual and operational framework to test hypotheses on site fidelity across systems and scales. 相似文献