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111.
Expression of betaine aldehyde dehydrogenase gene and salinity tolerance in rice transgenic plants 总被引:9,自引:0,他引:9
Betaine as one of osmolytes plays an important role in osmoregulation of most high plants. Betaine aldehyde dehydrogenase C BADH) is the second enzyme involved in betaine biosynthesis. The BADH gene from a halophite, Atriplex hortensis, was transformed into rice cultivars by bombarment method. Totally 192 transgenic rice plants were obtained and most of them had higher salt tolerance than controls. Among transgenic plants transplanted in the saline pool containing 0.5% NaCl in a greenhouse, 22 survived, 13 of which set seeds, and the frequency of seed setting was very low, only 10% . But the controls could not grow under the same condition. The results of BADH ac-tivity assay and Northern blot showed that the BADH gene was integrated into chromosomes of transgenic plants and expressed. 相似文献
112.
四个籼稻(Oryza sativa L.)品种幼苗经1℃黑暗或光照250 μm ol·m - 2·s- 1处理后,抗冷的“桂山矮选3”比不抗冷的“青华6 号”幼苗存活率高,其子代是以“桂山矮选3”为母本的比“青华6 号”为母本的存活率较高。抽穗期剑叶经光照低温处理12、24 和36 h 后,光合作用是“桂山矮选3”和以“桂山矮选3”为母本的子代比“青华6 号”和以“青华6 号”为母本的子代下降较少。呼吸作用是前者比后者在处理12 h 时有明显升高现象。荧光参数Fv/Fo和Fv/Fm比值在处理24 h 时前者比后者下降明显,但在常温下恢复则是前者比后者明显较快。自然低温(寒露风)对叶绿素荧光的影响亦有相似的规律。对水稻后代的抗冷性倾向于母本进行了讨论 相似文献
113.
M. S. Ghatge V. V. Deshpande P. K. Ranjekar 《World journal of microbiology & biotechnology》1995,11(2):144-147
Restriction analysis of the genomic DNA from a high glucose/xylose-isomerase-yieldingStreptomyces sp. NCIM 2730 revealed a number of distinct bands on a background smear, indicating the occurrence of repeated DNA sequences in the genome. Optical renaturation analysis indicated that 25% of the genome comprised rapidly reannealing sequences with a copy number of 50 and a kinetic complexity of 3×103. Hybridization of theStreptomyces genomic library with theStreptomyces DNA, supported the estimate of the repetitive DNA content derived from the re-association kinetics of the DNA. Hybridization of DNA from three differentStreptomyces species with a rice repetitive DNA probe revealed the presence of homologous sequences, which is a unique finding.M.S. Ghatge was and V.V. Deshpande and P.K. Ranjekar are with the Division of Biochemical Sciences, National Chemical Laboratory, Pune -41108, India; M.S. Ghatge is now with the Department of Microbiology & Molecular Biology, The University of Kansas Medical Center, 36th and Rainbow Blvd, Kansas City, Kansas - 66103, USA. 相似文献
114.
P. K. Lee J. A. Buswell K. Shinagawa 《World journal of microbiology & biotechnology》1995,11(6):696-698
Eleven of 16 samples of rice on sale in rice shops and supermarkets in Hong Kong contained Bacillus cereus. Although B. cereus counts did not exceed 100 bacteria/g in most of the positive samples, a sample of Thai red rice and a poor quality rice originating from China contained between 300 to 1000 cells/g and 104 to 2×105 cells/g, respectively. Nine strains produced an enterotoxin responsible for the diarrhoeal-type B. cereus food poisoning and seven of these strains also produced a haemolysin (haemolysin BL), a dermonecrotic vascular permeability factor which may be a virulence determinant in diarrhoeal illness caused by this bacterium.P.K. Lee and J.A. Buswell are with the Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong; K. Shinagawa is with the Department of Veterinary Medicine, Iwate University, Iwate, Japan. 相似文献
115.
116.
The results of studies on assimilate and water transport in the developing caryopsis of rice are summarised. Evidence is presented for a symplastic movement of solutes as far as the aleurone layer. However, transport into the apoplast at the nucellus/aleurone interface appears to be a necessary step due to the absence of plasmodesmata at this site. It is suggested that water leaves the caryopsis during grain filling by the isolated cell walls of the pigment strand, the suberised walls of these cells functioning to isolate the apoplast from the symplast and thereby allowing opposing fluxes of water and assimilates to occur in the dorsal region of the grain. 相似文献
117.
Submergence induces rapid internodal elongation in deep-water rice (Oryza sativa L. cv. Habiganj Aman II). We investigated the metabolic activities which help to support such fast growth. Three days of submergence in water under continuous light led to the mobilization of 65% of the starch from those regions of rice internodes which had been formed prior to submergence. Disappearance of starch was accompanied by a 70-fold enhancement of amylolytic activity. Similar increases in amylolytic activity were detected in response to ethylene and gibberellic acid. Submergence also caused a 26-fold increase in the translocation of newly synthesized photosynthetic assimilates from the leaves to the internodes and younger regions of the culms. These physiological processes are likely to provide the metabolic energy required for internodal elongation in response to submergence.Abbreviation GA3
gibberellic acid 相似文献
118.
Summary Application of a granular formulation of hexachlorocyclohexane (HCH) to the potted soil at flooding decreased the concentration
of iron and. to some extent, manganese in rice plants, especially at concentrations above 3 ppm active ingredient (a.i.) Likewise,
HCH, applied to rice fields at transplanting (several days after submergence) caused a significant decrease in the concentration
of iron, and not manganese, in the rice plant but only at concentrations above 12.5 kg a.i./ha despite high levels of reduced
iron in the soil. Inhibition of iron reduction by HCH was more pronounced when applied at flooding than at several days after
flooding. 相似文献
119.
Rino Cella Roberta Colombo Maria Grazia Galli Erik Nielsen Franco Rollo Francesco Sala 《Physiologia plantarum》1982,55(3):279-284
Rice ( Oryza sativa L.) cells returning to in vitro culture after preservation at superlow temperature in liquid nitrogen are characterized by a number of physiological alterations. These include: reduction in respiration and glucose uptake, loss of intracellular potassium, decrease in the cellular level of key metabolites (ATP, glucose-6-phosphate and pyruvate) and fragility of protoplasts following the action of cell wall-degrading enzymes.
Nevertheless, cell growth resumes after a short lag phase (2–4 days) with an actual 70–100% cell survival, thus indicating that the observed damage is not lethal and can be repaired in a short time. 相似文献
Nevertheless, cell growth resumes after a short lag phase (2–4 days) with an actual 70–100% cell survival, thus indicating that the observed damage is not lethal and can be repaired in a short time. 相似文献
120.
Two drought tolerant varieties TKM-1 and TKM-2 and two drought susceptible varieties Jaya and Improved Sabarmati of rice were studied for soluble protein pattern and isoenzymes of malate dehydrogenase, glutamate dehydrogenase, esterase and peroxidase during germination at different water stress. MDH, GDH and esterase patterns were not affected, but the soluble proteins were changed. Peroxidase isoenzyme pattern from drought tolerant and susceptible varieties showed characteristic differences. The intensity of bands with higher electrophoretic mobility decreased in Jaya and Improved Sabarmati while in TKM-1 and TKM-2 the intensity of these bands did not change much after 72 hr water stress. In shoots of Jaya and Improved Sabarmati, the activity of the peroxidase isoenzymes decreased more than in TKM-1 and TKM-2 shoots with increase in water stress. 相似文献