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81.
C. M. Berman 《International journal of primatology》1980,1(2):153-170
Several aspects of agonistic experience are described for freeranging infant rhesus monkeys (Macaca mulatta)on Cayo Santiago. Even before infants are fully integrated with peers in rank dominance based on maternal ranks,infants of highranking mothers tend to be threatened less frequently by other members of the group and are less likely to
be threatened by unfamiliar individuals than are infants of lowranking mothers. There is no evidence that fearful interactions
between pairs of infants are related to their mother’s ranks before 22 weeks of age. However, an imperfect hierarchy can be
constructed for infants between 27 and 30 weeks of age. At this age,infants of higherranking mothers are also more likely to receive protection when threatened than are infants of lowerranking
mothers. When protected, their protectors are less likely to emit fearful gestures to the infants’ threatener. Close female
relatives appear to play a large role in the protection of infants and may be more directly responsible for the differences
described above than the mother, other relatives, or other highranking members of the group. It is suggested that more than
one mechanism, including intervention by the mother and by close female relatives,may be important in rank acquisition among peers. 相似文献
82.
Daniel P. Rossignol Aimee J. Roschelle William J. Lennarz 《Journal of cellular biochemistry》1981,15(4):347-358
We have attempted to identify a surface component of echinoderm eggs that is involved in the species-specific binding of sperm. Cell surface membranes from eggs of the sea urchins Strongylocentrotus purpuratus or Arbacia punctulata were radioiodinated, detergent-treated, and subjected to density-gradient centrifugation. In the presence of bindin, the complementary binding protein isolated from sperm, one component of the membranes sedimented to a different density. This membrane component bound-species specifically to sperm that had undergone the acrosome reaction. This binding led to an inhibition of the ability of treated sperm to fertilize eggs. Exhaustive proteolytic digestion of this receptor fraction yields a high molecular weight glycopeptide that can also bind to bindin. It therefore appears that this egg surface membrane fraction contains a functionally intact, species-specific receptor for sperm. 相似文献
83.
Sea urchin sperm–egg adhesion is mediated by bindin, a sperm surface protein that has lectin-like activity. Bindin agglutinates eggs, and this interaction has been shown to be inhibited by glycopeptides released from the egg surface by protease treatment. In this study, we report the purification and properties of such an egg surface glycoconjugate that may be involved in sperm adhesion. The glycoconjugate was partially purified by gel filtration and affinity chromatography on bindin particles. Upon gel filtration on Sepharose CL 4-B, the glycoconjugate elutes near the void volume, suggesting that it has a molecular weight in excess of one million. In addition, we have found that the egg surface glycoconjugate agglutinates bindin particles, indicating that it is multivalent. Carbohydrate analysis indicates that the glycoconjugate is composed primarily of fucosc, xylose, galactose, and glucose. This purified egg surface component is the most potent inhibitor of bindin-mediated egg agglutination yet described. 相似文献
84.
Enrique J. del Pino Marcelo O. Cabada María C. Fonio 《Molecular reproduction and development》1981,4(2):97-104
Various plant lecins were employed in cell agglutination experiments to ascertain the presence of specific saccharides in the surface of B arenarum and L chaquensis spermatozoa. B arenarum spermatozoa were specifically agglutinated with Concanavalin A (Con A), phytohemagglutinin P (PHA-P), and wheat germ agglutinin (WGA), but not with soybean agglutinin (SBA). In contrast, L chaquensis spermatozoa were strongly agglutinated by SBA, WGA, and PHA-P. L chaquensis spermatozoa did not agglutinate with Con A even at high concentrations. Lectinmediated sperm agglutination was inhibited in the presence of specific lectinbinding sugars. Spermatozoa from both species were agglutinated randomly with all lectins suggesting a uniform distribution in the sperm surface of the lectinbinding saccharide ligands. B arenarum sperm agglutination induced by Con A is sensitive to temperature. B arenarum spermatozoa are more agglutinable at 24°C than at 4°C. These results suggest that lectin-binding site mobility is necessary for sperm agglutination. 相似文献
85.
H. Keith Massel Salvador Macias Darlene M. Meador Duane M. Rumbaugh 《International journal of primatology》1981,2(1):9-17
Twenty-month-old rhesus monkeys were tested in a modified discrimination-reversal paradigm, which was designed to distinguish
abstract learning from stimulus-response associational learning. Previous studies indicate that talapoin monkeys learn associationally
and great apes via forming abstract concepts. Adult rhesus monkeys are apparently capable of forming simple abstractions,
but learn primarily through associational process. The results of this study show the adolescent rhesus monkeys to be associational
learners, with their response patterns indicating more complexity than the talapoins but less than the adult rhesus monkeys.
The data suggest that rhesus monkeys develop their low-level capacity of abstract learning with maturation. 相似文献
86.
Meredith F. Small 《International journal of primatology》1981,2(1):91-95
Using weight and skinfold thickness to calculate relative body fat, the fat content of 21 captive female rhesus macaques was
estimated. Although age and. pregnancy had no effect on fat, rank was significant, especially during winter, with high-rank
females having the highest fat scores. Rank therefore has a significant effect on the health of captive animals and may also
affect the health of individuals in feral groups. 相似文献
87.
D. E. Brooks 《Molecular reproduction and development》1983,7(4):367-376
Tissue pieces from the caput epididymidis of the rat were incubated in vitro with (35S) methionine to produce radioactive secretory proteins. The radioactive secretory proteins so formed were tested for their ability to bind to washed rat spermatozoa collected from the rete testis and cauda epididymidis, and to rat erythrocytes. The sperm and erythrocytes bound approximately 5% of the total radioactive protein. Binding was protein-specific in that only selected proteins became associated with the cells. Binding was not cell-specific, however, since testicular spermatozoa, caudal spermatozoa, and erythrocytes all bound the same proteins to a similar degree. 相似文献
88.
Edith Wallace Harold I. Calvin George W. Cooper 《Molecular reproduction and development》1983,7(4):377-387
Three successive generations of mice were fed a Torula yeast based Se-deficient diet with or without 0.1 ppm Se in the drinking water. The Se-deficient mice, in the course of three generations, showed a decrease in body weight, testis weight, epididymal weight, and sperm production. The percentage of morphologically abnormal sperm increased in successive generations. The majority of sperm defects were found in the midpiece region of the tail. Many of these aberrant sperm were motile. A progressive decrease in fertility was noted during the first two generations of Se deficiency. This system confirms the essential role of Se in spermatogenesis and provides a model for the evaluation of the primary effect of Se deprivation on the structural development of sperm. 相似文献
89.
When the dry sperm of the sea urchin, Hemicentrotus pulcherrimus, were diluted 100 times in artificial sea water at 0°C and at 20°C, they became motile and the levels of ATP and creatine phosphate decreased rapidly. The level of ADP hardly changed, and the AMP level increased after the dilution. After the dilution, the respiratory rate at 2°C was almost one fifth that of 20°C. Both phospholipid and glycogen were used for the energy sources in sea urchin sperm. The level of phospholipid was 10-fold higher than that of glycogen in the dry sperm. The phospholipid level decreased after dilution at 20°C, though the level hardly changed at 0°C, suggesting that phospholipid was hardly metabolized the lower temperature. The level of α -glycerophosphate increased at 20°C after the dilution but did not change at 0°C. The level of glycogen decreased after the dilution, regardless of the temperature. The glycolysis was also activated after the dilution. Of the intermediates of the tricarboxylic acid cycle, the citrate concentration increased at 0°C and the malate concentration also increased at 0°C and especially strongly at 20°C. 相似文献
90.
M Schwerin S Blottner P D Thomsen D Roschlau G Brockmann 《Molecular reproduction and development》1991,30(1):39-43
An easy assay for quantification of Y chromosome-bearing sperm (Y-sperm) is needed, especially to monitor sperm separation techniques. In the present study a tritiated bovine male-specific DNA fragment was tested for identification of Y-sperm by in situ hybridization. A protocol for in situ hybridization to bovine sperm was developed and used to study the proportion of Y-sperm of 12 bulls. The usefulness of the method in optimization of sperm separation procedures is illustrated through analysis of fractions of sperm separated by Percoll density gradient centrifugation. 相似文献