全文获取类型
收费全文 | 12388篇 |
免费 | 913篇 |
国内免费 | 716篇 |
出版年
2024年 | 13篇 |
2023年 | 107篇 |
2022年 | 152篇 |
2021年 | 246篇 |
2020年 | 260篇 |
2019年 | 383篇 |
2018年 | 333篇 |
2017年 | 301篇 |
2016年 | 353篇 |
2015年 | 365篇 |
2014年 | 709篇 |
2013年 | 1029篇 |
2012年 | 620篇 |
2011年 | 485篇 |
2010年 | 469篇 |
2009年 | 621篇 |
2008年 | 646篇 |
2007年 | 669篇 |
2006年 | 619篇 |
2005年 | 535篇 |
2004年 | 490篇 |
2003年 | 467篇 |
2002年 | 395篇 |
2001年 | 295篇 |
2000年 | 267篇 |
1999年 | 293篇 |
1998年 | 224篇 |
1997年 | 189篇 |
1996年 | 183篇 |
1995年 | 221篇 |
1994年 | 228篇 |
1993年 | 192篇 |
1992年 | 175篇 |
1991年 | 141篇 |
1990年 | 143篇 |
1989年 | 108篇 |
1988年 | 90篇 |
1987年 | 88篇 |
1986年 | 82篇 |
1985年 | 130篇 |
1984年 | 145篇 |
1983年 | 114篇 |
1982年 | 108篇 |
1981年 | 88篇 |
1980年 | 73篇 |
1979年 | 61篇 |
1978年 | 54篇 |
1977年 | 24篇 |
1976年 | 18篇 |
1973年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 531 毫秒
981.
Two independent components of mating behaviour, sexual selection and assortative mating, were studied in two allopatric morphs, one sheltered boulder shore form (S-morph) and one exposed cliff shore form (E-morph), of Littorina saxatilis from the west coast of Sweden. Sexual selection was studied by comparing the sizes of copulating and non-copulating snails in the field. Size assortative mating was studied by collecting copulating pairs in the field, while assortative mating between morphs was investigated by bringing the pure morphs together in intermediary habitats and then noting the matings. The S-morph mated randomly in relation to size in two of the studied populations and exhibited a trend towards size assortative mating in a third, while the E-morph showed size assortative mating in both studied populations. The microdistribution of sizes of snails on the shores could not explain all the size assortative mating found, and instead it is argued that a size-based mate rejection behaviour also contributes to the assortative mating in at least some of these populations. There was sexual selection on size in both males and females in the S-morph, with large individuals being favoured as mates. In contrast, copulating snails of the E-morph were smaller than non-copulating ones. The significantly different sexual selection intensities between the two morphs may help to explain the size differences between them. There was random mating between the E- and the S-morphs of L. saxatilis, which suggests no incipient reproductive isolation between morphs on Swedish rocky shores. This is in agreement with earlier studies of Swedish populations, but is in contrast to the situation found in other geographical areas. 相似文献
982.
F. A. Huntingford V. A. Braithwaite J. D. Armstrong D. Aird K. E. Thorpe P. Joiner 《Journal of fish biology》1998,53(2):314-321
This paper describes an experimental study of the effects of food supply, growth rates and social interactions on homing by juvenile Atlantic salmon Salmo salar in response to displacement. Groups of five fish were housed in a section of an artificial stream and given either rations allowing maximum growth (the rich condition) or 0·1 of this amount (the poor condition); daily specific growth rates were significantly higher in the rich condition. After a 6-day settlement period, the fish were captured, displaced downstream and their movements recorded over the next 3 h. Prior to displacement, the fish showed a high degree of site fidelity and high levels of aggression. Dominant fish and those with stronger site attachment grew faster prior to displacement, these effects being independent. Following displacement, 24% of all fish returned to their previously favoured site and stayed there, 23% returned home initially, but subsequently moved on, 5% settled in a new site and 49% failed to move. The distribution of responses was identical for the rich and poor conditions, but fish that homed were dominant and had grown faster during the pre-displacement period. 相似文献
983.
I. Métais C. Aubry B. Hamon D. Peltier R. Jalouzot 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):232-237
We describe the cloning and the characterization of a 130-bp DNA fragment, called OPG9-130, amplified from bean (Phaseolus vulgaris L.) genomic DNA. This fragment corresponds to a minisatellite DNA sequence containing seven repeats of 15 bp which differ
slightly from each other in their sequence. Southern analysis showed that the core sequence of 15 bp is repeated in clusters
dispersed throughout the genome. The use of this fragment as a probe allowed us to identify common bean lines by their DNA
fingerprints. We suggest that OPG9-130 will be useful for line identification as well as for the analysis of genetic relatedness
between bean species and lines.
Received: 14 February 1998 / Accepted: 10 February 1998 相似文献
984.
To evaluate the effect of balancing selection and intragenic recombination (or gene conversion) at six individual HLA loci, synonymous nucleotide diversity in different exon groups is examined within (πw) and between (πb) allelic lineages that may be defined by either serological or DNA sequence differences. Both π values are high in exons
which encode for the peptide binding region (PBR) and tend to decrease in other exons. The value of πw is significantly smaller than that of πb in any exon of any locus. However, even πw is much greater than nucleotide diversity at non-HLA loci. These observations provide additional strong evidence for the operation of balancing selection in PBR-encoding exons
and its indirect effects on polymorphism at linked neighboring regions. It appears that allelic lineages have generally evolved
in isolation but the linkage relationships within and between exons are incomplete throughout the long evolutionary history.
To quantify intragenic recombination and account for the large discrepancy between the HLA and non-HLA diversity, a population genetics model is analyzed with special reference to the evolution of modern humans. The analysis
suggests that the recombination rate between two sites 1000 base pairs apart is about 10–5 per generation and that the effective size of human populations (equivalent roughly to the number of breeding individuals
in a randomly mating population) has dropped from 105 to 104 in most of the Quaternary. One possibility for this reduction is discussed.
Received: 11 August 1997 / Revised: 8 October 1997 相似文献
985.
Different sugars, Gal, GalNAc and Man were docked at the monosaccharide binding sites of Erythrina corallodenron (EcorL), peanut lectin (PNA), Lathyrus ochrus (LOLI), and pea lectin (PSL). To study the lectin-carbohydrate interactions, in the complexes, the hydroxymethyl group in Man and Gal favors, gg and gt conformations respectively, and is the dominant recognition determination. The monosaccharide binding site in lectins that are specific to Gal/GalNAc is wider due to the additional amino acid residues in loop D as compared to that in lectins specific to Man/Glc, and affects the hydrogen bonds of the sugar involving residues from loop D, but not its orientation in the binding site. The invariant amino acid residues Asp from loop A, and Asn and an aromatic residue (Phe or Tyr) in loop C provides the basic architecture to recognize the common features in C4 epimers. The invariant Gly in loop B together with one or two residues in the variable region of loop D/A holds the sugar tightly at both ends. Loss of any one of these hydrogen bonds leads to weak interaction. While the subtle variations in the sequence and conformation of peptide fragment that resulted due to the size and location of gaps present in amino acid sequence in the neighborhood of the sugar binding site of loop D/A seems to discriminate the binding of sugars which differ at C4 atom (galacto and gluco configurations). The variations at loop B are important in discriminating Gal and GalNAc binding. The present study thus provides a structural basis for the observed specificities of legume lectins which uses the same four invariant residues for binding. These studies also bring out the information that is important for the design/engineering of proteins with the desired carbohydrate specificity. 相似文献
986.
Peter W. Schiller Ralf Schmidt Grazyna Weltrowska Irena Berezowska Thi M.-D. Nguyen Sébastien Dupuis Nga N. Chung Carole Lemieux Brian C. Wilkes Katharine A. Carpenter 《Letters in Peptide Science》1998,5(2-3):209-214
Novel conformationally constrained opioid peptide analogs with antagonist, mixed agonist/ antagonist or agonist properties were developed. TIP(P)-related antagonists showed unprecedented antagonist potency and receptor selectivity, and may have potential for use in analgesia in combination with agonists. A definitive model of their receptor-bound conformation was developed. Three prototype mixed agonist/ antagonists were discovered. They represent the only known compounds with this pharmacological profile and, as expected, one of them was shown to be a potent analgesic and to produce no dependence and less tolerance than morphine. Novel dipeptide derivatives turned out to be potent and selective agonists. Because of their low molecular weight and lipophilic character, these compounds may cross the blood-brain barrier and, thus, may have potential as centrally acting analgesics. 相似文献
987.
988.
The T-DNA tagged mutant gene of Arabidopsis thaliana, mei1, produces after meiosis an abnormal tetrad, consisting of five to eight microspores of varying sizes and DNA contents. Plant
DNA flanking the inserted T-DNA was isolated by inverse PCR. An approximately 16-kb DNA fragment spanning the T-DNA insertion
site was isolated by screening a wild-type genomic library, using the plant flanking DNA as a probe. Using RT-PCR and RNA
isolated from very young flower buds, a cDNA fragment was obtained. Nucleotide sequence comparison of the cDNA and the genomic
sequence in this region indicated a gene which contained two introns. The 5′ and 3′ splice sites of neither intron comply
with the :GU...AG: rule. In the mutant, the T-DNA had inserted into one of the introns. The deduced sequence of the MEI1 wild-type gene, which contains 89 amino acids, shows possible similarity with the human acrosin-trypsin inhibitor, HUSI-II,
and is about the same size. Two wild-type DNA fragments, both extending over the T-DNA insertion site, were introduced into
mutant plants by Agrobacterium-mediated transformation and plants were selected for both hygromycin and kanamycin resistance. Several independent male-fertile
transformants were obtained with one of the DNA fragments. The fragment showing complementation of the mutant phenotype indicated
that the sequence with similarity to the acrosin-trypsin inhibitor is MEI1. Within the 16-kb genomic fragment two other genes were identified; one showed no overall similarity to any protein sequence
in the database and the other had almost complete identity with an Arabidopsis-transcribed sequence tag with similarity to ACC oxidase. Double mutants between mei1 and qrt1 were made, permitting better characterization of the mei1 phenotype because the individual microspores continued to be held together after callose dissolution.
Received: 21 April 1998 / Revision accepted: 11 June 1998 相似文献
989.
The use of zinc(II) to probe iron binding and oxidation by the ferritin (EcFtnA) of Escherichia coli
Amyra Treffry Z. Zhao M. A. Quail John R. Guest P. M. Harrison 《Journal of biological inorganic chemistry》1998,3(6):682-688
The ferritin of Escherichia coli (EcFtnA) is similar to human H-chain ferritin (HuHF) in having 24 subunits, each containing a dinuclear site at which two
iron atoms can be oxidised (the diiron centre). In EcFtnA, unlike HuHF, fluorescence quenching of Trp122, located near site
A of the dinuclear centre, can be used to monitor metal binding (this tryptophan is absent from HuHF). Metal binding also
perturbs the UV absorbance spectrum of Trp122 and that of Tyr24 (a conserved residue near site B of the dinuclear centre).
Using UV-difference spectroscopy and fluorescence quenching it is shown that Fe(II) and Zn(II) bind at the same sites, A and
B. Sequential stopped-flow studies of Fe(II) binding and oxidation also show that Zn(II) is an effective competitor of Fe(II)
binding and an inhibitor of its oxidation.
Received: 10 June 1998 / Accepted: 18 September 1998 相似文献
990.
Active-site mobility in human immunodeficiency virus, type 1, protease as demonstrated by crystal structure of A28S mutant. 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Protein science : a publication of the Protein Society》网站下载免费的PDF全文](/ch/ext_images/free.gif)
L. Hong J. A. Hartsuck S. Foundling J. Ermolieff J. Tang 《Protein science : a publication of the Protein Society》1998,7(2):300-305
The mutation Ala28 to serine in human immunodeficiency virus, type 1, (HIV-1) protease introduces putative hydrogen bonds to each active-site carboxyl group. These hydrogen bonds are ubiquitous in pepsin-like eukaryotic aspartic proteases. In order to understand the significance of this difference between HIV-1 protease and homologous, eukaryotic aspartic proteases, we solved the three-dimensional structure of A28S mutant HIV-1 protease in complex with a peptidic inhibitor U-89360E. The structure has been determined to 2.0 A resolution with an R factor of 0.194. Comparison of the mutant enzyme structure with that of the wild-type HIV-1 protease bound to the same inhibitor (Hong L, Treharne A, Hartsuck JA, Foundling S, Tang J, 1996, Biochemistry 35:10627-10633) revealed double occupancy for the Ser28 hydroxyl group, which forms a hydrogen bond either to one of the oxygen atoms of the active-site carboxyl or to the carbonyl oxygen of Asp30. We also observed marked changes in orientation of the Asp25 catalytic carboxyl groups, presumably caused by the new hydrogen bonds. These observations suggest that catalytic aspartyl groups of HIV-1 protease have significant conformational flexibility unseen in eukaryotic aspartic proteases. This difference may provide an explanation for some unique catalytic properties of HIV-1 protease. 相似文献