全文获取类型
收费全文 | 16761篇 |
免费 | 1824篇 |
国内免费 | 2172篇 |
出版年
2024年 | 69篇 |
2023年 | 341篇 |
2022年 | 364篇 |
2021年 | 489篇 |
2020年 | 624篇 |
2019年 | 736篇 |
2018年 | 662篇 |
2017年 | 732篇 |
2016年 | 738篇 |
2015年 | 662篇 |
2014年 | 815篇 |
2013年 | 1162篇 |
2012年 | 798篇 |
2011年 | 813篇 |
2010年 | 646篇 |
2009年 | 813篇 |
2008年 | 802篇 |
2007年 | 899篇 |
2006年 | 790篇 |
2005年 | 709篇 |
2004年 | 599篇 |
2003年 | 599篇 |
2002年 | 532篇 |
2001年 | 411篇 |
2000年 | 391篇 |
1999年 | 395篇 |
1998年 | 314篇 |
1997年 | 266篇 |
1996年 | 336篇 |
1995年 | 281篇 |
1994年 | 256篇 |
1993年 | 266篇 |
1992年 | 199篇 |
1991年 | 214篇 |
1990年 | 170篇 |
1989年 | 162篇 |
1988年 | 152篇 |
1987年 | 157篇 |
1986年 | 147篇 |
1985年 | 164篇 |
1984年 | 171篇 |
1983年 | 110篇 |
1982年 | 147篇 |
1981年 | 89篇 |
1980年 | 114篇 |
1979年 | 93篇 |
1978年 | 74篇 |
1977年 | 68篇 |
1976年 | 54篇 |
1974年 | 39篇 |
排序方式: 共有10000条查询结果,搜索用时 93 毫秒
101.
Subcellular Distribution of UDP-Galactose:Ceramide Galactosyltransferase in Rat Brain Oligodendroglia 总被引:4,自引:3,他引:1
Oligodendrocytes isolated from 18-19-day-old rat brain were homogenized in 0.32 M sucrose. The homogenate was centrifuged at 100,000 g for 50 min in a gradient containing 0.8, 1.05, and 1.3 M sucrose. Three discrete bands were obtained at the interfaces 0.32-0.8 (F1), 0.8-1.05 (F2), and 1.05-1.3 M (F3). The distribution of UDP-galactose:ceramide galactosyltransferase (CgalT) activity in each fraction was measured using liposomes containing normal fatty acid-containing ceramides (NFA-CgalT activity) or 2-hydroxy fatty acid-containing ceramides (HFA-CgalT activity). Although detection of both CgalT activities was possible in all fractions, HFA-CgalT activity was enriched in F1 and F2 fractions, which also showed an enrichment of Golgi and endoplasmic reticulum markers, respectively. It is interesting that NFA-CgalT activity was significantly enriched in the F2 fraction. These results suggest that hydroxylated and nonhydroxylated galactocerebrosides may be synthesized at different intracellular locations. 相似文献
102.
Vera Yip Mary Ellen Pusateri Joyce Carter Irwin A. Rose Oliver H. Lowry 《Journal of neurochemistry》1988,50(2):594-602
The distribution of glucose-1,6-bisphosphate (G16P2) synthase was measured in more than 70 regions of mouse brain, and nine layers of monkey retina. Activities in gray areas varied as much as 10-fold, in a hierarchical manner, from highest in telencephalon, especially the limbic system, to lowest in cerebellum, medulla, and spinal cord. The synthase levels were significantly correlated among different regions with G16P2 itself, as well as with previously published levels of a brain specific IMP-dependent G16P2 phosphatase. In contrast, neither G16P2 nor either its synthase or phosphatase correlated positively with phosphoglucomutase, and in all regions the G16P2 levels greatly exceeded requirements for activation of this mutase. This strengthens the view that G16P2 has some function besides serving as coenzyme for phosphoglucomutase. However, attempts to correlate the "G16P2 system," as defined by the three coordinately related elements, synthase, phosphatase, and G16P2, with other enzymes of carbohydrate metabolism, or with regional data of Sokoloff et al. [J. Neurochem. 28, 897-916 (1977)] for glucose consumption, were unsuccessful. This leaves open the possibility that brain G16P2 might serve as a phosphate donor for specific nonmetabolic effector proteins. 相似文献
103.
Studies on the Tissue Distribution of the Puromycin-Sensitive Enkephalin-Degrading Aminopeptidases 总被引:3,自引:0,他引:3
Stacey McLellan Simon H. Dyer Gerry Rodriguez Louis B. Hersh 《Journal of neurochemistry》1988,51(5):1552-1559
An antiserum generated to the soluble form of the rat brain puromycin-sensitive enkephalin-degrading aminopeptidase was used to determine the tissue distribution of the soluble and membrane-associated forms of this enzyme. All tissues examined contained significant levels of the soluble enzyme form, with this enzyme accounting for greater than 90% of the arylamidase activity in brain, heart, and skeletal muscle. Native gel electrophoresis coupled with activity staining as well as inhibition studies were used to confirm the presence of this enzyme in various tissues. Serum was found not to contain this particular aminopeptidase. In contrast to the results obtained with the soluble enzyme form, brain was the only tissue found to contain the membrane-associated enzyme form. Although all tissues contained membrane-associated aminopeptidase activity only the brain enzyme could be maintained in solution in the absence of detergent. In addition, the brain membrane-associated enzyme could be distinguished from the membrane-associated aminopeptidase activity in other tissues on the basis of its sensitivity to inhibition by puromycin. 相似文献
104.
Summary
Dalbergia sissoo, a wind-dispersed tropical tree, shows a positively skewed distribution of seeds per pod. This is attributed to the enhanced dispersal advantage of few-seeded pods due to their reduced wing loading (ratio of weight to pod surface area) and low settling velocity. The proximate mechanisms causing the positively skewed distribution were investigated. The distribution could not be attributed to the distribution pattern of ovule number per ovary, pollen grain limitation, lack of ovule fertilization, or post-fertilization elimination of many-seeded pods. Rather, it was caused by the post-fertilization abortion of seeds within a pod 2 weeks after fertilization. This intra-pod seed abortion (IPSA) is due to a dominance hierarchy of fertilized ovules from the distal (near stigma) to the basal end, generated by the temporal differences in fertilization. The dominant developing seeds at the distal end cause the abortion of others through the production and diffusion of an aborting agent. When the dominance hierarchy of the siblings is not intense, pods are formed with more than one seed. We argue that the positively skewed distribution of seeds per pod is not due to maternal regulation but is a result of sibling rivalry. We propose that this sibling rivalry is generated by genetic differences in pollen grain fitness and disucss the results in the context of parent-offspring conflict. 相似文献
105.
This research was conducted on a captive group of eight wild boars: Four wildborn adults (one male and three females) and four piglets (one male and three females), born in captivity two months before the beginning of the study. They were housed in the Rome Zoo in a 1,000 sq m enclosure furnished with trees, mud pools, a water basin, and two small barns. Data on eight behavioral states (sleeping, resting, rooting, eating, walking, standing, scratching, and nursing) were collected by instantaneous sampling at 10-minute intervals. The spatial location of each subject was also noted, as well as closeness to conspecifics. Results showed that adults and piglets differed in their activity budgets. Activity patterns showed a significant correlation with the mean temperatures. Furthermore, there was a significant difference in the use of the exhibit areas between adults and piglets. This study suggests a social structure in which the core is constituted by females and their yearlings. The nonbreeding female has a more marginal position than the breeding ones. Finally, the male tends to be even more peripheral and solitary. Our results are partly consistent with the data obtained in studies on free-ranging wild boars (Mauget, 1980; Dardaillon, 1984). 相似文献
106.
Four aerial survey projects were conducted between 1977 and 1986 to determine the abundance, density and distribution of West Indian manatees (Trichechus manatus), in the northern Banana River, Kennedy Space Center, Florida. Manatee density and distribution within selected portions of the 78.5 km2 study area were determined. Peak numbers of manatees occurred in spring of each year. The maximum counts increased from 56 in 1978 to 297 in 1986. Manatee abundance was lowest in the winter of each year. Mean density per flight increased from 0.52 manatees/km2 in 1977–78 to 2.73/km2 in 1984–86. This increase may reflect increases in the east coast population or shifts in the population distribution. Distributional changes were observed in the study area through time, with a lower percentage of manatees occurring in industrial areas and a correspondingly higher percentage of manatees in nonindustrial areas by 1985. 相似文献
107.
Takashi Kohyama 《Journal of plant research》1988,101(2):207-212
A simple relation (v-lnB) (u-lnx)=c was recognized betweenx as dbh (or individual basal area) andB as the cumulative basal area abovex, for various stands of warm-temperate rain forests of Yakushima Island, southern Japan (v, u andc, parameters). The model parameters explained a difference in the pattern of stand development between secondary succession
after clear-felling and gap regeneration within primary forests. 相似文献
109.
Summary Adult size (in terms of dry weight; DW) and development time (T
p
) of the solitary parasitoidAphidius ervi varied when reared in different nymphal instars of its host, apterous virginoparae of the pea aphid (Acyrthosiphon pisum). Parasitoid DW increased with an increase in the DW of the host at parasitization, from the first to the third aphid instar. Female wasps gained 1.1 times more in DW than their male counterparts in all four host classes, butT
p
did not significantly differ between the sexes. Parasitoid DW was consistently more variable thanT
p
. The two traits covaried positively with an increase in host size from the first to the third instar, but they varied independently in parasitoids from fourth-instar hosts. The host size (and stage) at the time of parasitization imposes constraints on the growth and development of immatureA. ervi that are reflected in the pattern of covariation between DW andT
p
. When growing in aphids below a certain size threshold, parasitoids can maximize fitness by a trade-off between DW andT
p
. Consequently, the assumption implicit in host-size models of parasitoid oviposition decisions — that females incur a relatively greater reduction in size (used as an index of fecundity) than males when developing in poor quality hosts — can be falsified. 相似文献
110.
Clifford A. Behrens 《Human ecology: an interdisciplinary journal》1992,20(4):435-462
Traditionally, the Shipibo economy was subsistence-based with shifting cultivation supplying calories, principally in the form of plantains and root crops, while fishing and hunting provided animal proteins to the diet. Some men, who recently began producing rice for sale in regional markets, now allocate less time to wild food procurement. Moreover, this trend has been accompanied by the nucleation of households, a growing cash market for agricultural labor, and the intravillage sale of faunal foods. This paper shows that with cash cropping, some Shipibo now freely distribute less food to others in relation to the amount they produce. To account for this change, a theory is developed based on time allocation and patterns of economic behavior reported throughout the Amazon. This theory is then applied to explain specialization and the formation of cash markets for food labor among the Shipibo. 相似文献