全文获取类型
收费全文 | 17786篇 |
免费 | 1449篇 |
国内免费 | 1126篇 |
出版年
2024年 | 35篇 |
2023年 | 310篇 |
2022年 | 340篇 |
2021年 | 532篇 |
2020年 | 591篇 |
2019年 | 864篇 |
2018年 | 725篇 |
2017年 | 701篇 |
2016年 | 657篇 |
2015年 | 643篇 |
2014年 | 806篇 |
2013年 | 1536篇 |
2012年 | 533篇 |
2011年 | 712篇 |
2010年 | 620篇 |
2009年 | 897篇 |
2008年 | 972篇 |
2007年 | 863篇 |
2006年 | 876篇 |
2005年 | 701篇 |
2004年 | 746篇 |
2003年 | 602篇 |
2002年 | 563篇 |
2001年 | 419篇 |
2000年 | 406篇 |
1999年 | 378篇 |
1998年 | 382篇 |
1997年 | 314篇 |
1996年 | 314篇 |
1995年 | 296篇 |
1994年 | 249篇 |
1993年 | 235篇 |
1992年 | 234篇 |
1991年 | 191篇 |
1990年 | 152篇 |
1989年 | 164篇 |
1988年 | 115篇 |
1987年 | 131篇 |
1986年 | 101篇 |
1985年 | 116篇 |
1984年 | 79篇 |
1983年 | 36篇 |
1982年 | 48篇 |
1981年 | 55篇 |
1980年 | 33篇 |
1979年 | 20篇 |
1978年 | 21篇 |
1977年 | 13篇 |
1976年 | 10篇 |
1972年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
992.
Gert‐Jan Bekker Benson Ma Narutoshi Kamiya 《Protein science : a publication of the Protein Society》2019,28(2):429-438
Single‐domain antibodies (sdAbs) function like regular antibodies, however, consist of only one domain. Because of their low molecular weight, sdAbs have advantages with respect to production and delivery to their targets and for applications such as antibody drugs and biosensors. Thus, sdAbs with high thermal stability are required. In this work, we chose seven sdAbs, which have a wide range of melting temperature (Tm) values and known structures. We applied molecular dynamics (MD) simulations to estimate their relative stability and compared them with the experimental data. High‐temperature MD simulations at 400 K and 500 K were executed with simulations at 300 K as a control. The fraction of native atomic contacts, Q, measured for the 400 K simulations showed a fairly good correlation with the Tm values. Interestingly, when the residues were classified by their hydrophobicity and size, the Q values of hydrophilic residues exhibited an even better correlation, suggesting that stabilization is correlated with favorable interactions of hydrophilic residues. Measuring the Q value on a per‐residue level enabled us to identify residues that contribute significantly to the instability and thus demonstrating how our analysis can be used in a mutant case study. 相似文献
993.
Ann‐Christin Moritzer Hartmut H. Niemann 《Protein science : a publication of the Protein Society》2019,28(12):2112-2118
Flavin‐dependent halogenases require reduced flavin adenine dinucleotide (FADH2), O2, and halide salts to halogenate their substrates. We describe the crystal structures of the tryptophan 6‐halogenase Thal in complex with FAD or with both tryptophan and FAD. If tryptophan and FAD were soaked simultaneously, both ligands showed impaired binding and in some cases only the adenosine monophosphate or the adenosine moiety of FAD was resolved, suggesting that tryptophan binding increases the mobility mainly of the flavin mononucleotide moiety. This confirms a negative cooperativity between the binding of substrate and cofactor that was previously described for other tryptophan halogenases. Binding of substrate to tryptophan halogenases reduces the affinity for the oxidized cofactor FAD presumably to facilitate the regeneration of FADH2 by flavin reductases. 相似文献
994.
Takefumi Yamashita Eiichi Mizohata Satoru Nagatoishi Takahiro Watanabe Makoto Nakakido Hiroko Iwanari Yasuhiro Mochizuki Taisuke Nakayama Yuji Kado Yuki Yokota Hiroyoshi Matsumura Takeshi Kawamura Tatsuhiko Kodama Takao Hamakubo Tsuyoshi Inoue Hideaki Fujitani Kouhei Tsumoto 《Structure (London, England : 1993)》2019,27(3):519-527.e5
995.
《Structure (London, England : 1993)》2019,27(9):1405-1415.e5
- Download : Download high-res image (254KB)
- Download : Download full-size image
996.
997.
998.
999.
1000.