全文获取类型
收费全文 | 10549篇 |
免费 | 1851篇 |
国内免费 | 2596篇 |
专业分类
14996篇 |
出版年
2024年 | 127篇 |
2023年 | 379篇 |
2022年 | 279篇 |
2021年 | 307篇 |
2020年 | 588篇 |
2019年 | 599篇 |
2018年 | 698篇 |
2017年 | 660篇 |
2016年 | 648篇 |
2015年 | 661篇 |
2014年 | 685篇 |
2013年 | 860篇 |
2012年 | 539篇 |
2011年 | 601篇 |
2010年 | 414篇 |
2009年 | 561篇 |
2008年 | 526篇 |
2007年 | 567篇 |
2006年 | 553篇 |
2005年 | 485篇 |
2004年 | 406篇 |
2003年 | 408篇 |
2002年 | 397篇 |
2001年 | 329篇 |
2000年 | 276篇 |
1999年 | 277篇 |
1998年 | 224篇 |
1997年 | 189篇 |
1996年 | 185篇 |
1995年 | 195篇 |
1994年 | 188篇 |
1993年 | 149篇 |
1992年 | 175篇 |
1991年 | 112篇 |
1990年 | 107篇 |
1989年 | 79篇 |
1988年 | 87篇 |
1987年 | 55篇 |
1986年 | 54篇 |
1985年 | 68篇 |
1984年 | 51篇 |
1983年 | 27篇 |
1982年 | 53篇 |
1981年 | 33篇 |
1980年 | 33篇 |
1979年 | 27篇 |
1978年 | 22篇 |
1977年 | 13篇 |
1976年 | 18篇 |
1958年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
121.
122.
Carbon accumulation and distribution in Pinus massoniana and Cunninghamia lanceolata mixed forest ecosystem in Daqingshan, Guangxi, China 总被引:1,自引:0,他引:1 下载免费PDF全文
Carbon accumulation and distribution were studied at three sampling plots in a 13-year-old mixed planatation of Pinus massoniana and Cunninghamia lanceolata in Daqingshan, Guangxi, China. The results showed that carbon content varied with tissues and tree species, but the total carbon content of Pinus massoniana was higher than that of Cunninghamia lanceolata. The average tissue carbon contents of Pinus massoniana were: wood (58.6%) > root (56.3%) > branch (51.2%) > bark (49.8%) > leaf (46.8%), while those of Cunninghamia lanceolata were: bark (52.2%) > leaf (51.8%) > wood (50.2%) > root (47.5%) > branch (46.7%). The carbon contents of the soil (at a depth of 60cm) ranged from 1.45% to 1.84% with an average of 1.70%. Carbon contents were higher in the surface soil (0–20cm) than in the deep layer (below 20cm). The average carbon contents were the highest for trees (51.1%), followed by litter (48.3%), shrubs (44.1%), and herbs (33.0%). The biomass of the trees in the three plots ranged from 85.35 t hm-2 to 101.35 t hm-2 with an average of 93.83 t hm-2, in which 75.7%–82.6% was Pinus massoniana. The biomass of the understory was 2.10–3.95 t hm-2 with an average of 2.72 t hm-2, while the standing stock of ground litter was 5.49–7.91 t hm-2 with an average of 6.75 t hm-2. The carbon storage in the mixed plantation reached the maximum in the soil layer (69.02%), followed by vegetation (29.03%), and standing litter (1.82%). The carbon storage in the tree layer occupied 23.90% of the total ecosystem and 97.7% of the vegetation layer. Pinus massoniana accounted for 65.39% of the total carbon storage in the tree layer. Tissue carbon storage was directly related to the corresponding amount of biomass. Trunks had the highest carbon storage, accounting for 53.23% of the trees in Pinus massoniana and 55.57% in Cunninghamia lanceolata, respectively. Roots accounted for about 19.22% of the total tree carbon. The annual net productivity of the mixed plantation was 11.46 t hm-2a-1, and that of sequestered carbon was 5.96 t hm-2a-1, which was equivalent to fixing CO2 of 21.88 t hm-2a-1. The plantation was found to be an important sink of atmospheric CO2. 相似文献
123.
The invasion of woody plants into grass‐dominated ecosystems has occurred worldwide during the past century with potentially significant impacts on soil organic carbon (SOC) storage, ecosystem carbon sequestration and global climate warming. To date, most studies of tree and shrub encroachment impacts on SOC have been conducted at small scales and results are equivocal. To quantify the effects of woody plant proliferation on SOC at broad spatial scales and to potentially resolve inconsistencies reported from studies conducted at fine spatial scales, information regarding spatial variability and uncertainty of SOC is essential. We used sequential indicator simulation (SIS) to quantify spatial uncertainty of SOC in a grassland undergoing shrub encroachment in the Southern Great Plains, USA. Results showed that both SOC pool size and its spatial uncertainty increased with the development of woody communities in grasslands. Higher uncertainty of SOC in new shrub‐dominated communities may be the result of their relatively recent development, their more complex above‐ and belowground architecture, stronger within‐community gradients, and a greater degree of faunal disturbance. Simulations of alternative sampling designs demonstrated the effects of spatial uncertainty on the accuracy of SOC estimates and enabled us to evaluate the efficiency of sampling strategies aimed at quantifying landscape‐scale SOC pools. An approach combining stratified random sampling with unequal point densities and transect sampling of landscape elements exhibiting strong internal gradients yielded the best estimates. Complete random sampling was less effective and required much higher sampling densities. Results provide novel insights into spatial uncertainty of SOC and its effects on estimates of carbon sequestration in terrestrial ecosystem and suggest effective protocol for the estimating of soil attributes in landscapes with complex vegetation patterns. 相似文献
124.
A Tomiyama K Tachibana K Suzuki S Seino J Sunayama K-i Matsuda A Sato Y Matsumoto T Nomiya K Nemoto H Yamashita T Kayama K Ando C Kitanaka 《Cell death & disease》2010,1(7):e60
Recently developed heavy ion irradiation therapy using a carbon beam (CB) against systemic malignancy has numerous advantages. However, the clinical results of CB therapy against glioblastoma still have room for improvement. Therefore, we tried to clarify the molecular mechanism of CB-induced glioma cell death. T98G and U251 human glioblastoma cell lines were irradiated by CB, and caspase-dependent apoptosis was induced in both cell lines in a dose-dependent manner. Knockdown of Bax (BCL-2-associated X protein) and Bak (BCL-2-associated killer) and overexpression of Bcl-2 or Bcl-xl (B-cell lymphoma-extra large) showed the involvement of Bcl-2 family proteins upstream of caspase activation, including caspase-8, in CB-induced glioma cell death. We also detected the activation of extracellular signal-regulated kinase (ERK) and the knockdown of ERK regulator mitogen-activated protein kinase kinase (MEK)1/2 or overexpression of a dominant-negative (DN) ERK inhibited CB-induced glioma cell death upstream of the mitochondria. In addition, application of MEK-specific inhibitors for defined periods showed that the recovery of activation of ERK between 2 and 36 h after irradiation is essential for CB-induced glioma cell death. Furthermore, MEK inhibitors or overexpression of a DN ERK failed to significantly inhibit X-ray-induced T98G and U251 cell death. These results suggested that the MEK–ERK cascade has a crucial role in CB-induced glioma cell death, which is known to have a limited contribution to X-ray-induced glioma cell death. 相似文献
125.
凋落物分解速率及其温度敏感性Q_(10)能够影响凋落物对土壤的碳归还及其对全球变暖的响应。然而,凋落物有机碳质量对凋落物分解及其温度敏感性的影响研究仍不充分。以黄河三角洲芦苇(Phragmites australi)为例,通过凋落物袋法、室内模拟实验及固态~(13)C核磁共振技术,研究有机碳质量对凋落物分解及其温度敏感性的影响,探讨预测凋落物分解及其温度敏感性的指标。结果表明:(1)随着凋落物分解,易分解碳组分(烷氧碳、双烷氧碳)相对含量逐渐降低,而难分解碳组分(芳香碳)相对含量显著增加,疏水碳/亲水碳、芳香碳/烷氧碳比值逐渐增大,凋落物有机碳更加稳定,凋落物呼吸速率及失重率呈下降趋势。(2)凋落物失重主要受烷基碳、烷氧碳相对含量及C/N的影响,凋落物CO_2累积释放量主要受烷氧碳及双烷氧碳相对含量的影响。羰基碳相对含量可以用来解释Q_(10)的变异。因此,相对于生态化学计量比,烷基碳、烷氧碳、双烷氧碳、羰基碳相对含量是预测凋落物分解及其温度敏感性的敏感性指标。 相似文献
126.
127.
Xian-Hao Cheng Shun-Xing Guo Chun-Lan Wang 《植物学报(英文版)》2006,48(11):1312-1317
The effects of substrate composition and temperature on myceilal growth and sclerotium production in Grlfola umbellate (Pers.) Pilaet were Investigated In the present study. The Induction of sclerotla of G. umbellate was affected greatly by the type of medium, as well as the type of carbon source. Malt-extract agar was able to induce the production of sclerotia. The production of sclerotia was also observed when the carbon source in the GPC agar medium (glucose 20 g/L, peptone 6 g/L, corn steep liquor 10 g/L, and agar 15 g/L) was replaced with glycerol or mannitol. Altering the composition of the GPC medium with milk powder, thiamine hydrochlorlde, extract of Armlllarla mellea, active clay, dlatomite, kaolin, or arginlne did not induce the production of sclerotla. A temperature range of 18-25 ℃ was suitable for both mycellai growth and sclerotium formation. Glycerol significantly Induced slerotium formation on nutrient supplemented with sawdust substrates In bottle culture. 24S-Polyporusterone A and polyporusterone B were assayed In samples of natural and cultured sclerotla. Both natural and cultured sclerotla contained 24S- polyporusterone A and polyporusterone B. 相似文献
128.
Lipozyme IM20 from Novo Nordisk (Denmark) was examined after various treatments. Conditions were chosen to reflect those that would be considered in the design of an industrial process. A two-level factorial design was employed to assess the effects of pressurization/depressurization cycles, rate of depressurization and exposure length. A significant three-factor interaction was observed. Lowest residual activity was observed for runs in which the depressurization rate was 86–89 bar min–1. Incubation for 12 h also yielded low residual activity but only when exposing the immobilized enzyme to one cycle. The highest residual activity was obtained for immobilized enzymes repeatedly exposed for periods of 12 h (5 times) with a depressurization rate of 4.3 to 4.45 bar min–1. This effect may be due to the extraction of an inhibiting compound. Tuning process parameters can lead to a seven-fold change in residual activity. 相似文献
129.
Leila Feiz Rosalind Williams‐Carrier Susan Belcher Monica Montano Alice Barkan David B. Stern 《The Plant journal : for cell and molecular biology》2014,80(5):862-869
Ribulose‐1,5‐bisphosphate carboxylase/oxygenase (Rubisco) plays a critical role in sustaining life by catalysis of carbon fixation in the Calvin–Benson pathway. Incomplete knowledge of the assembly pathway of chloroplast Rubisco has hampered efforts to fully delineate the enzyme's properties, or seek improved catalytic characteristics via directed evolution. Here we report that a Mu transposon insertion in the Zea mays (maize) gene encoding a chloroplast dimerization co‐factor of hepatocyte nuclear factor 1 (DCoH)/pterin‐4α‐carbinolamine dehydratases (PCD)‐like protein is the causative mutation in a seedling‐lethal, Rubisco‐deficient mutant named Rubisco accumulation factor 2 (raf2‐1). In raf2 mutants newly synthesized Rubisco large subunit accumulates in a high‐molecular weight complex, the formation of which requires a specific chaperonin 60‐kDa isoform. Analogous observations had been made previously with maize mutants lacking the Rubisco biogenesis proteins RAF1 and BSD2. Chemical cross‐linking of maize leaves followed by immunoprecipitation with antibodies to RAF2, RAF1 or BSD2 demonstrated co‐immunoprecipitation of each with Rubisco small subunit, and to a lesser extent, co‐immunoprecipitation with Rubisco large subunit. We propose that RAF2, RAF1 and BSD2 form transient complexes with the Rubisco small subunit, which in turn assembles with the large subunit as it is released from chaperonins. 相似文献
130.
Can we measure terrestrial photosynthesis from space directly,using spectral reflectance and fluorescence? 总被引:2,自引:0,他引:2
J. GRACE C. NICHOL M. DISNEY† P. LEWIS† T. QUAIFE† P. BOWYER† 《Global Change Biology》2007,13(7):1484-1497
Attempts to estimate photosynthetic rate or gross primary productivity from remotely sensed absorbed solar radiation depend on knowledge of the light use efficiency (LUE). Early models assumed LUE to be constant, but now most researchers try to adjust it for variations in temperature and moisture stress. However, more exact methods are now required. Hyperspectral remote sensing offers the possibility of sensing the changes in the xanthophyll cycle, which is closely coupled to photosynthesis. Several studies have shown that an index (the photochemical reflectance index) based on the reflectance at 531 nm is strongly correlated with the LUE over hours, days and months. A second hyperspectral approach relies on the remote detection of fluorescence, which is a directly related to the efficiency of photosynthesis. We discuss the state of the art of the two approaches. Both have been demonstrated to be effective, but we specify seven conditions required before the methods can become operational. 相似文献