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41.
The only known population of Batrachospermum spermatiophorum Vis et Sheath, located in a small stream on east Maui, Hawaii, was sampled from November 2001 to October 2002 to investigate its phenology. Additionally, the taxonomic status of the alga was examined. Phylogenetic analyses of the large subunit of the ribulose‐1,5‐bisphosphate carboxylase/oxygenase (rbcL) gene for B. spermatiophorum confirm its placement within the Section Contorta of the genus Batrachospermum. Comparisons with rbcL gene sequences for all other available Batrachospermum species, including 10 other members of the Section Contorta, illustrate that B. spermatiophorum is distinct in its rbcL nucleotide sequence and should be maintained as a separate species. The phenology of B. spermatiophorum differed from most studies of Batrachospermum reproduction in that the gametophytes were present year round, and no macroscopic chantran‐sia stages were identified at any point during the present study. Maximum plant length was highest in the late fall and early winter months, while gametangial production peaked in February and March. Carposporophyte abundance rose sharply in July and August, and the percentage of carposporophytes producing carpospores was highest in late summer and early fall months. Although significant correlations were determined among stream conditions and among phenological characteristics, only pH was associated with several of the reproductive characters, which is unusual for studies of Batrachospermum phenology. Timing of sexual reproduction for all macroalgal community members in the stream site was not coordinated, with some reproducing year round and others more sporadically. The year‐round presence of Batrachospermum gametophytes may be a result of a combination of smaller seasonal changes in cues such as day length and water temperature in the tropical environment than occur in temperate locations, and lack of extreme conditions that might inhibit the persistence of the gametophyte.  相似文献   
42.
Morphological observations and molecular analyses of the north‐western Pacific species of the red algal genus Grateloupia (Halymeniaceae) indicate the presence of an entity, which is somewhat similar in gross morphology to G. asiatica Kawaguchi et Wang but is distinguished from the latter species by some morphological features. These include: (i) a somewhat fleshy texture; (ii) wider and much thicker (4.5–10 mm wide and up to 1300 μm thick) axes, of which an inner cortex consists of more (6–9) cells; (iii) generally longer (up to 17 cm), marginal and surface proliferations that are clearly constricted (terete) at bases; and (iv) much elongated, oblong auxiliary cells. Phylogenetic analysis using the ribulose‐l,5‐bisphosphate carboxylase/ oxygenase (rbcL) gene of G. asiatica and the alga in question shows them to be distantly related and strongly supports the differentiation of these two entities at the species level. Judging from the literature, this entity is actually Grateloupia subpectinata Holmes, which has been placed into synonymy under G. asiatica [as G. filicina (Lamouroux) C. Agardh] or G. prolongata J. Agardh in previous reports, and therefore the Holmes name is reinstated.  相似文献   
43.
Abstract An invasive, cold‐tolerant strain of the tropical green alga Caulerpa taxifolia was introduced recently in the Mediterranean Sea and along the Californian coast. We screened 50 aquarium and open‐sea C. taxifolia specimens for the presence/absence of an intron located in the rbcL gene of chloroplast DNA. We also reanalysed a total of 229 sequences of the Internal Transcribed Spacer (ITS) of ribosomal DNA, combining previously published sequences from different studies with 68 new sequences to complement rbcL data. The introduced Mediterranean strain was found to be characterized by the absence of the rbcL intron and by the occurrence of a particular monomorphic ITS type. A PCR assay based on rbcL gene was developed to detect new introductions of the invasive strain of C. taxifolia. This rapid and inexpensive test could be useful to assist environment managers in the preservation of coastal marine ecosystems.  相似文献   
44.
rbcL (1310 bp) and matK (1014 bp), using 15 species representing the family. The study included analyses of Ticodendron (Ticodendraceae) and three species of Betulaceae as close relatives, and one species each of Juglandaceae and Myricaceae as outgroups. Analyses based on matK gene sequences, which provided a much better resolution than the analyses based on rbcL gene sequences alone, resulted in a single most parsimonious tree whose topology is almost identical with the strict consensus tree generated by the combined data set of rbcL and matK gene sequences. Results showed that Casuarinaceae are monophyletic, comprising four distinct genera, Allocasuarina, Casuarina, Ceuthostoma and Gymnostoma, which were not recognized until recently. Within the family, Gymnostoma is positioned at the most basal position and sister to the remainder. Within the remainder Ceuthostoma is sister to the Allocasuarina-Casuarina clade. Morphologically the basalmost position of Gymnostoma is supported by plesiomorphies such as exposed stomata in the shallow longitudinal furrows of the branchlets, a basic chromosome number x=8 and the gynoecium composed of two fertile, biovulate carpels. The three other genera, Allocasuarina, Casuarina, and Ceuthostoma, have invisible stomata in the deep longitudinal furrows of the branchlets, a higher basic chromosome number x=9 or 10–14 (unknown in Ceuthostoma), the gynoecium composed of one fertile and one sterile carpel with a single ovule (unknown in Ceuthostoma). The diversity of infructescence morphology found in the latter three genera suggests that they may have evolved in close association with the elaboration of fruit dispersal mechanisms. Received 14 September 2001/ Accepted in revised form 12 October 2001  相似文献   
45.
 DNA sequences of both 5′ and 3′ regions of the plastid ndhF gene were generated in order to study the position of Patrinia and Nardostachys, to check the potential paraphyletic nature of Patrinieae, and to evaluate the possible link between the tribe and Linnaeaceae. Parsimony analysis showed very strong support for Patrinia as sister to all members of Valerianaceae (including Nardostachys) and indicated the paraphyletic nature of the tribe Patrinieae. Additionally, trees were constructed from available rbcL data separately and supplemented with ndhF sequences. Topologies of these combined cladograms are in agreement with the ndhF phylogeny, suggesting that the traditionally circumscribed Patrinieae can no longer be recognized but must be considered as part of a basal grade in Valerianaceae. Parsimony analysis based on a morphological data set supported a monophyletic Patrinieae; combination with the molecular data showed a paraphyletic Patrinieae. Furthermore, the possible link between Patrinieae and Linnaeaceae is evaluated. Received July 12, 2001 Accepted February 25, 2002  相似文献   
46.
The seven currently recognized species of Geranium endemic to the Hawaiian Islands are unusual in their shrubby or arborescent habit and unlobed, parallel-veined leaves rather than the palmately cleft or lobed leaves and herbaceous habit typical of the genus. Their placement within the genus and their biogeographic source have been obscured by this morphological distictiveness and the limited resolution of relationships on the basis of morphology in the very speciose subgenus Geranium. Phylogenetic analysis of rbcL gene sequences provides strong support for the monophyly of the Hawaiian group, and indicates that the Hawaiian clade is deeply nested within section Geranium rather than comprising a separate section. The continental relatives studied to date with the greatest similarity in sequence to the Hawaiian group are native to the Americas rather than Asia or the Pacific. The Hawaiian species are extremely similar to one another in rbcL sequence, while the tree topology obtained is consistent with a basal position for Geranium arboreum within the group.  相似文献   
47.
Editorial note     
Phylogenetic analyses of the Dasyaceae based on sequence analysis of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) and 42 morphological characters are presented. Comparative sequence analysis confirms the general view of the Ceramiaceae as a primitive, paraphyletic group giving rise to the Rhodomelaceae, Delesseriaceae and Dasyaceae within the monophyletic Ceramiales. On the basis of both data sets, the Heterosiphonia-like genera (Heterosiphonia, Colacodasya and Dasyella) are the most primitive members of the Dasyaceae, whereas the Dasya-like genera (Dasya, Pogonophorella, Eupogodon and Rhodoptilum) and Thuretia and Dictyurus are of more recent origin. On the basis of morphological data only, Thuretia and Dictyurus form a sister group to Heterosiphonia, and Eupogodon is monophyletic whereas Dasya and Heterosiphonia are not. Primary radial symmetry has arisen once in the Dasya clade but is secondarily obscured in some species by heavy, asymmetrical cortication that gives the appearance of bilateral symmetry. This is illustrated by species of Eupogodon and Rhodoptilum.  相似文献   
48.
49.
 The phylogeny of the genus Gunnera is investigated for the first time. Twelve species representing the six currently recognised subgenera are analysed. Two chloroplast DNA regions, the rbcL gene and the rps16 intron, together provide 46 informative characters out of 2335. A combined analysis of both genes gives four most parsimonious trees, firmly establishing the east South American G. herteri as sister group to the rest of the genus. The African G. perpensa is sister group to two well-supported clades, one including the South American subgenera Misandra and Panke, the other the Australian/New Zealand/Malayan species of subgenera Milligania and Pseudogunnera. Thus, South America is a composite area for Gunnera, showing up at two different levels in the cladogram. Our analysis supports a close biogeographic relationship between Australia and New Zealand. The evolution of some morphological characters is discussed. Lastly, the unusual structure of some of the rbcL sequences is reported. Received July 6, 2000 Accepted October 24, 2000  相似文献   
50.
Abstract: At the C-terminal end of Rubisco's large subunit major differences in sequence length and in charges of the amino acid residues occur in unicellular organisms and in plants. This C-terminal segment of the large subunit participates in large movements during the catalytic cycle. It participates in the closing mechanism of the binding niche for the substrate RuBP, changing from an ordered structure in the "open" enzyme conformation to a position, stretched over the protein surface, in a "closed" conformation. We analyzed the sequence variability in the C terminus in rbcL to investigate whether this structurally important entity evolved in an ordered process. Cyanobacteria and chlorophytes show similar C-terminal sequences (DXX), whereby D-473 is the last strictly conserved amino acid residue for all rbcLs . Contrary to the gymnosperms (D + 2 residues), the C termini of the angiosperms show variable lengths from D + 2 to D + 17 residues. The plant orders of Asterales, Batales, Cap-parales, Caryophyllales, Fabales, Gentianales, Lamiales, Ru-biales, Myrtales, Scrophulariales, and Solanales contain species with particularly elongated C termini. Recent studies regarding enzyme kinetics demonstrated that molecules with longer C termini are better adapted for a wider temperature range. We speculate that longer C termini confer properties to the enzyme that modulate the success of different species in different environments. This is supported by the fact that "modern" (e.g., phylogenetically young taxa in an actual radiation process) generally display a long C terminus, while conservative taxa have a relatively short C terminus.  相似文献   
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