全文获取类型
收费全文 | 338篇 |
免费 | 18篇 |
国内免费 | 19篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 7篇 |
2020年 | 8篇 |
2019年 | 14篇 |
2018年 | 17篇 |
2017年 | 10篇 |
2016年 | 9篇 |
2015年 | 6篇 |
2014年 | 24篇 |
2013年 | 34篇 |
2012年 | 14篇 |
2011年 | 22篇 |
2010年 | 11篇 |
2009年 | 17篇 |
2008年 | 12篇 |
2007年 | 14篇 |
2006年 | 16篇 |
2005年 | 10篇 |
2004年 | 14篇 |
2003年 | 11篇 |
2002年 | 14篇 |
2001年 | 9篇 |
2000年 | 4篇 |
1999年 | 8篇 |
1998年 | 7篇 |
1997年 | 5篇 |
1996年 | 6篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 6篇 |
1991年 | 1篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1984年 | 5篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 3篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 2篇 |
排序方式: 共有375条查询结果,搜索用时 31 毫秒
151.
ReportofSixRareCasesofAbnormalKaryotypesLiGuixinSongGuangjiangSongXingjun(BiologyDepartment,TaishanMedicalCollege,Taiah,Shandong271000)我定在对外遗传咨询并进行染色体检查时,发现6例异常核型,经医学细胞遗传学国家培训中心夏家辉等专家鉴定,为世界首报枝型.现报告如下:例1女,26岁,表型正常.妊娠4次,均于3个月自然流产.无任何诱因.常规染色体检查,核型为46,XX但有两条异常染色体.经G显带分析,异常核型为46,XX,t(1;8)(lqter-1p32::8q22-8qter;8pier-8q22::1p32-1pter)(图1).例2女,25岁… 相似文献
152.
Itiseasytoderiveprimarymyoblastsfromhumanbiopsy.Thetransformedmyoblastscouldexpressforeigngeneathighlevel.Butlessmyoblastsexistinadults[1],andareliabletodifferentiateandfailtodivide,leadingtolowefficiencyoftransfectionandamplificationinvitro,andsubsequen… 相似文献
153.
The influence of quaternary structure on the low frequency molecular vibrations of the haem within deoxyhaemoglobin (deoxy Hb) and Oxyhaemoglobin (oxy Hb) was studied by resonance Raman scattering. The FeO2 stretching frequency was essentially identical between the high affinity (R) state (Hb A) and low affinity (T) state (Hb Kansas and Hb M Milwaukee with inositol hexaphosphate). However in deoxy Hb, only one of the polarized lines showed an appreciable frequency shift upon switch of quaternary structure, i.e. 215 to 218 cm?1 for the T state (Hb A, des-His(146β) Hb, and des-Arg(141α) Hb (pH 6.5)) and 220 to 221 cm?1 for the R state (des-Arg(141α) Hb (pH 9.0), des-His(146β)-Arg(141α) Hb and NES des-Arg(141α) Hb). Based on the observed 54Fe isotopic frequency shift of the corresponding Raman lines of deoxy Hb A (214 → 217 cm?1), of deoxy NES des-Arg Hb (220 → 223 cm?1), of the protoporphyrinato-Fe(II)-(2-methylimidazole) complex in the ferrous high spin state (207 → 211 cm?1) and of deoxymyoglobin (220 → 222 cm?1) (Kitagawa et al., 1979), and on substitution of perdeuterated for protonated 2-methylimidazole in the deoxygenated picket fence complex (TpivPP)Fe2+ (2-MeIm) (209 → 206 cm?1), and on the results of normal co-ordinates calculation carried out previously, we proposed that the 216 cm?1 line of deoxy Hb is associated primarily with the FeNε(HisF8) stretching mode and accordingly that the FeNε(HisF8) bond is stretched in the T state due to a strain exerted by globin. 相似文献
154.
Cartilage fibrils contain collagen II as the major constituent, but the presence of additional components, minor collagens, and noncollagenous glycoproteins is thought to be crucial for modulating several fibril properties. We have examined the distribution of two fibril constituents—decorin and collagen IX—in samples of fibril fragments obtained after bovine cartilage homogenization. Decorin was preferentially associated with a population of thicker fibril fragments from adult articular cartilage, but was not present on the thinnest fibrils. The binding was specific for the gap regions of the fibrils, and depended on the decorin core protein. Collagen IX, by contrast, predominated in the population with the thinnest fibrils, and was scarce on wider fibrils. Double-labeling experiments demonstrated the coexistence of decorin and collagen IX in some fibrils of intermediate diameter, although most fibril fragments from adult cartilage were strongly positive for one component and lacked the other. Fibril fragments from fetal epiphyseal cartilage showed a different pattern, with decorin and collagen IX frequently colocalized on fragments of intermediate and large diameters. Hence, the presence of collagen IX was not exclusive for fibrils of small diameter. These results establish that articular cartilage fibrils are biochemically heterogeneous. Different populations of fibrils share collagen II, but have distinct compositions with respect to macromolecules defining their surface properties. 相似文献
155.
The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wild-type strain CA274 or in visA mutants that had been rendered light-resistant by introduction of the wild-type visA+ gene. Accumulation of Proto IX was also not observed in cells with a mutation in the visB gene. These results confirm the hypothesis that the sensitivity of the visA mutants to light is caused by the abnormal accumulation of Proto IX, a substrate of ferrochelatase, as the result of a genetic defect in the gene for ferrochelatase. 相似文献
156.
W D Horrocks R F Venteicher C A Spilburg B L Vallee 《Biochemical and biophysical research communications》1975,64(1):317-322
Apomyoglobin has been reconstituted with the lanthanide porphyrin complex, ytterbium(III)mesoporphyrin IX. The reconstituted material exhibits absorption and magnetic circular dichroic spectra significantly different from those of the ytterbium porphyrin itself. The sizeable, positive extrinsic Cotton effect in the Soret band of Yb-mesoporphyrin IX induced by the interactions with the globin indicates that the lanthanide porphyrin complex occupies the heme crevice. 相似文献
157.
The activity of a methyltransferase, BchU, which catalyzes methylation at the C-20 position of chlorin ring in the biosynthetic pathway of bacteriochlorophyll c, was investigated in vitro. The bchU gene derived from the photosynthetic green sulfur bacterium, Chlorobium tepidum, was overexpressed in Escherichia coli as a His-tagged protein (His(6)-BchU), and the enzyme was purified. In the presence of S-adenosylmethionine, His(6)-BchU methylated zinc bacteriopheophorbide d at the C-20 position to give zinc bacteriopheophorbide c. Metal-free bacteriopheophorbide d could not be methylated by the BchU, indicating that the central metal in the chlorin should be required for the recognition by the BchU. 相似文献
158.
Iwata H Kaibara M Dohmae N Takio K Himeno R Kawakami S 《Biochemical and biophysical research communications》2004,316(1):65-70
In our previous papers, we reported that factor IX (F-IX), when activated by erythrocyte membranes, causes coagulation. We report on purification, identification, and characterization of F-IX-activating enzyme extracted from human erythrocyte membranes. The enzyme whose amino acid sequence is almost in accord with neutrophil elastase was found in normal erythrocyte membrane. The molecular mass was slightly smaller than that of neutrophil elastase. The content of the enzyme in erythrocyte membranes was estimated to be 3.0-3.7 ng per 10(6)erythrocytes. The F-IX sites cleaved by the enzyme were slightly different from those by the ordinary coagulation reaction. The ability of F-IX cleaved by the enzyme to cause coagulation was estimated to be approximately 1/10 as high as that of the F-IX cleaved by activated F-XI. These findings provide evidence that F-IX is activated by erythrocyte membrane, which may serve as a triggering mechanism for blood coagulation. 相似文献
159.
Hypoxia activates the capacity of tumor-associated carbonic anhydrase IX to acidify extracellular pH 总被引:1,自引:0,他引:1
Svastová E Hulíková A Rafajová M Zat'ovicová M Gibadulinová A Casini A Cecchi A Scozzafava A Supuran CT Pastorek J Pastoreková S 《FEBS letters》2004,577(3):439-445
Acidic extracellular pH (pHe) is a typical attribute of a tumor microenvironment, which has an impact on cancer development and treatment outcome. It was believed to result from an accumulation of lactic acid excessively produced by glycolysis. However, metabolic profiles of glycolysis-impaired tumors have revealed that CO2 is a significant source of acidity, thereby indicating a contribution of carbonic anhydrase (CA). The tumor-associated CA IX isoform is the best candidate, because its extracellular enzyme domain is highly active, expression is induced by hypoxia and correlates with poor prognosis. This study provides the first evidence for the role of CA IX in the control of pHe. We show that CA IX can acidify the pH of the culture medium in hypoxia but not in normoxia. This acidification can be perturbed by deletion of the enzyme active site and inhibited by CA IX-selective sulfonamides, which bind only to hypoxic cells containing CA IX. Our findings suggest that hypoxia regulates both expression and activity of CA IX in order to enhance the extracellular acidification, which may have important implications for tumor progression. 相似文献
160.
Saban SD Nepomuceno RR Gritton LD Nemerow GR Stewart PL 《Journal of molecular biology》2005,349(3):526-537
We report a sub-nanometer resolution cryo-electron microscopy (cryoEM) structural analysis of an adenoviral vector, Ad35F, comprised of an adenovirus type 5 (Ad5) capsid pseudo-typed with an Ad35 fiber. This vector transduces human hematopoietic cells via association of its fiber protein with CD46, a member of the complement regulatory protein family. Major advances in data acquisition and image processing allowed a significant improvement in resolution compared to earlier structures. Analysis of the cryoEM density was enhanced by docking the crystal structures of both the hexon and penton base capsid proteins. CryoEM density was observed for hexon residues missing from the crystal structure that include hypervariable regions and the epitope of a neutralizing monoclonal antibody. Within the penton base, density was observed for the integrin-binding RGD loop missing from the crystal structure and for the flexible beta ribbon of the variable loop on the side of the penton base. The Ad35 fiber is flexible, consistent with the sequence insert in the third beta-spiral repeat. On the inner capsid surface density is revealed at the base of the hexons and below the penton base. A revised model is presented for protein IX within the virion. Well-defined density was assigned to a conserved domain in the N terminus of protein IX required for incorporation into the virion. For the C-terminal domain of protein IX two alternate conformations are proposed, either binding on the capsid surface or extending away from the capsid. This model is consistent with the tolerance of the C terminus for inserted ligands and its potential use in vector retargeting. This structural study increases our knowledge of Ad capsid assembly, antibody neutralization mechanisms, and may aid further improvements in gene delivery to important human cell types. 相似文献