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51.
E. Grist A. D. Woodhead C. Carlson 《In vitro cellular & developmental biology. Plant》1986,22(11):677-680
Summary Several established cell lines from different classes of vertebrates were assayed for the presence of O6-methylguanine acceptor protein. This protein is instrumental in removing adducts from DNA caused by exposure to alkylating
agents. Cultured cells had levels of acceptor protein activity within the range found in fresh tissues from animals in the
same class. We suggest that cells from lower vertebrates are satisfactory in vitro models for studies of this DNA repair function. 相似文献
52.
W. L. C. Vaz D. Hallmann R. M. Clegg A. Gambacorta M. De Rosa 《European biophysics journal : EBJ》1985,12(1):19-24
We have used the fluorescence recovery after photobleaching technique to study the translational diffusion, in L phase multibilayers of 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), of fluorescent derivatives of 1-palmitoyl-2-oleoylphosphatidylethanolamine (NBD-POPE) and a membrane-spanning phosphatidylethanolamine (NBD-MSPE). The latter derivative was prepared from a membrane-spanning glycerol-dialkyl-glycerol tetraether lipid isolated from the thermophilic and acidophilic archaebacterium Sulfolobus solfataricus. The translational diffusion was examined between about 15° and 45°C. It is shown that over this temperature range the translational diffusion coefficient for NBD-MSPE is 2/3 that for NBD-POPE which spans only one monolayer of the bilayer. The result is interpreted in terms of existing models for translational diffusion in lipid membranes.Abbreviations D
t
translational diffusion coefficient
- FRAP
fluorescence recovery after photobleaching
- MSPE
a membrane-spanning phosphatidylethanolamine derived from a glycerol-dialkyl-glycerol tetraether lipid isolated from Sulfolobus solfataricus
- NBD
4-nitrobenz-2-oxa-1,3-diazolyl
- PE
phosphatidylethanolamine
- POPC
1-palmitoyl-2-oleoylphosphatidylcholine
- POPE
1-palmitoyl-2-oleoylphosphatidylethanolamine 相似文献
53.
M. G. Roberts 《Journal of mathematical biology》1985,22(3):303-311
A general formulation for a family of cyclic epidemic models with density-dependent feedback mechanisms and removed classes is presented. A parameter, , related to the basic reproductive rate determines the asymptotic behaviour of solutions of the model. It is shown that if <1 the trivial solution is globally stable, and if >1 it is conditionally stable. The results are applied to a set of differential equations that has been used to model the life cycle of a parasite that has two hosts. 相似文献
54.
Summary A general method is presented for the formulation and numerical evaluation of mathematical models describing epithelial transport. The method is based on the principles of conservation of mass, and maintenance of electroneutrality within the cells and bathing solutions. It is therefore independent of the specific membrane transport mechanisms, and can be used to evaluate different models describing arbitrary transport processes (including passive, active and cotransport processes). Detailed numerical methods are presented that allow computation of steady-state and transient responses under open-circuit, current-clamp and voltage-clamp conditions, using a general-purpose laboratory minicomputer. To evaluate the utility of this approach, a specific model is presented that is consistent with the Koefoed-Johnson and Ussing hypothesis of sodium transport in tight epithelia (Acta Physiol. Scand.
42:298–308, 1958). This model considers passive transport of an arbitrary number of permeant solutes, active transport of sodium and potassium, and osmotically induced water transport across the apical and basolateral membranes. Results of the model are compared to published experimental measurements in rabbit urinary bladder epithelium. 相似文献
55.
Motility patterns of caput epididymal chimpanzee sperm, caput epididymal chimpanzee sperm incubated in vitro with chimpanzee cauda epididymal fluid, and cauda epididymal chimpanzee sperm were assessed quantitatively. Sperm recovered from the caput epididymis showed no motility, whereas sperm recovered from cauda epididymis showed progressive forward motility. After incubation in cauda fluid, approximately 25% of caput epididymal sperm showed some motile activity. Electrophoretic analysis of 125I-labeled sperm plasma membrane preparations revealed that the surface of caput epididymal sperm, incubated in cauda fluid, was modified by the appearance of a major protein-glycoprotein surface component with an apparent molecular weight of 27 kilodaltons (kD). THis 27-kD component was not detected on caput epididymal sperm incubated in buffer or in caput fluid. However, it was present in cauda fluid and on cauda epididymal sperm. Binding to caput epididymal sperm was cell specific in that chimpanzee erythrocytes incubated in cauda fluid did not bind this 27-kD cauda fluid component. Motility patterns of ejaculated chimpanzee sperm and of ejaculated chimpanzee sperm incubated in the uterus of adult female chimpanzees also were assessed quantitatively. Ejaculated sperm showed progressive forward motility, whereas in utero incubated ejaculated sperm showed hyperactivated motility typical of capacitated sperm. Electrophoretic analysis of 125I-labeled sperm plasma membrane preparations revealed the loss of a 27-kD component from the surface of ejaculated sperm after in utero incubation. No significant change in the 125I-distribution pattern was detectable when ejaculated sperm were incubated in buffer. These results suggest that the lumenal fluid component, which becomes adsorbed to the surface of chimpanzee sperm during maturation in the epididymis and which is removed from the surface of mature chimpanzee sperm in the female reproductive tract, affects sperm motility. 相似文献
56.
Host defense deficiency in newborn nonhuman primate lungs 总被引:2,自引:0,他引:2
A T Cheung G Kurland M E Miller E W Ford S A Ayin E M Walsh 《Journal of medical primatology》1986,15(1):37-47
We have investigated two major aspects of the pulmonary host defense mechanism--alveolar macrophage function as a "first line of bacterial defense" and induced neutrophil migration into the lung as a "back-up defense." Chemotactic and phagocytic/killing assays revealed a functional deficiency in the alveolar macrophages of newborn primates. Serial bronchoalveolar lavage investigations revealed diminished neutrophil migration into the newborn primate lung. The overall pulmonary host defense capability in newborn primates was deficient. The results of this investigation may have direct clinical relevance to the susceptibility of newborns to infections and pneumonia. 相似文献
57.
In the use of age structured population models for agricultural applications such as the modeling of crop-pest interactions it is often essential that the model take into account the distribution in maturation rates present in some or all of the populations. The traditional method for incorporating distributed maturation rates into crop and pest models has been the so-called distributed delay method. In this paper we review the application of the distributed delay formalism to the McKendrick equation of an age structured population. We discuss the mathematical properties of the system of ordinary differential equations arising out of the distributed delay formalism. We then discuss an alternative method involving modification of the Leslie matrix. 相似文献
58.
Patterns of food evacuation in fishes: a critical review 总被引:5,自引:0,他引:5
Lennart Persson 《Environmental Biology of Fishes》1986,16(1-3):51-58
59.
To investigate the role of satellite DNA in eukaryotic genomes, we isolated from an African green monkey (Cercopithecus aethiops) genomic library cloned segments containing the previously described deca-satellite linked to low-copy-number genomic sequences. Three such clones were obtained. The low-copy-number sequences in the three clones do not cross-hybridize suggesting that they derive from different genomic loci. The structure of one of the clones, λAMkA, is described in detail. Subcloned segments containing the low-copy-number sequences from λAMkA anneal to monkey, human and mouse genomic DNA. The subcloned probes were used to select clones containing homologous sequences from a second, independent monkey library as well as from human and mouse genomic libraries. Several of the newly isolated monkey clones hybridized to probes containing the species-specific deca- and -satellites, confirming the genomic association of the low-copy-number sequence in λAMkA with satellite DNA. Moreover, several of the human and mouse clones hybridized to species-specific human and mouse satellite DNAs, respectively. These experiments indicate that the low-copy-number sequence in λMkA and its association with satellite DNA is conserved in primates and rodents. 相似文献
60.
岛屿生物地理学理论:模型与应用 总被引:18,自引:1,他引:17
前言岛屿有许多显著特征,如地理隔离,生物类群简单。这些特点为重复性研究和统计学分析奠定了基础,从而有利于许多深入而细致的生物学研究。因此,岛屿为发展和检验自然选择、物种形成及演化,以及生物地理学和生态学诸领域的理论和假设,提供了重要的自然实验室。岛屿生物地理学理论(MacArthurwilson学说)即为岛屿生物学研究中所产生的著名理论之一。该理论发展之 相似文献