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91.
A K/Rb isotope dilution method was used to determine the uptake of K from undisturbed subsoils. Rb was applied to the topsoil
(0–30 cm) to trace the K taken up from the topsoil by crops. The K/Rb ratio in the crops increases when roots contact the
Rb-free subsoil. This change in the K/Rb ratio enables the calculation of the uptake of K from the subsoil.
Results of 34 field experiments on loess-parabrown soils in N. Germany showed that the subsoil (>30 cm) supplied, on average,
34% of the total K uptake by spring wheat (range 9–70%).
The range between the experimental sites is considered in relation to the contents of K in the top and subsoils (as extracted
by 0.025 N CaCl2 solution), the proportion of the total root length in the subsoils, and competition for K between roots in the top and subsoil.
In subsoils with similar K contents, uptake from the subsoil decreased significantly from 65 to 21% of total K uptake, as
K contents in the topsoils increased from 4 to 8 mg K/100 g.
On sites with the same K contents in topsoils (9 mg K/100 g), the subsoil supplied 12 to 61% of total K uptake as the K contents
of the subsoil increased from 2 to 27 mg K/100 g.
The contribution of uptake of K from the subsoil increased with the development of the crop, from 8% at first node stage to
35% at ear emergence, as the proportion of total root length in the subsoil increased.
High root length densities in the topsoil (9 cm/cm3) resulted in competition for K between roots and increased uptake of K from the subsoil. 相似文献
92.
93.
P. G. Arnison P. Donaldson L. C. C. Ho W. A. Keller 《Plant Cell, Tissue and Organ Culture》1990,20(3):147-155
Embryo formation by cultured broccoli (Brassica oleracea L. var. italica) anthers was best in the pH range of 5.5 to 5.8. Manipulation of the initial medium pH showed, however, that embryos could be recovered throughout the entire pH range tested. Experiments designed to test the influence of anther density on embryo production exhibited an apparent population effect. Comparison of anthers cultured with and without filaments showed a significantly lower level of embryo formation with filaments attached. The importance of anther orientation with the adaxial surface up was also demonstrated. Detailed studies of the effect of temperature on anther response showed the importance of 35°C treatments. Other temperatures and a variety of temperature manipulations were either comparatively ineffective or inhibitory. The duration of 35°C exposure required for optimal response varied widely between 18 and 48 h. Wide variation in plant to plant response was observed despite attempts to optimize the manipulation of physical parameters. Individual plants were identified that reliably formed many thousands of embryos, whereas other plants failed to form embryos under all tested conditions. 相似文献
94.
Angie Rizzino Peter Kazakoff John Nebelsick 《In vitro cellular & developmental biology. Plant》1990,26(5):537-542
Summary Previous studies have shown that cell density can regulate the binding of several growth factors. To determine whether cell
density exerts a uniform effect on the expression of epidermal growth factor (EGF) receptors, seven cell lines were examined
in detail. For each cell line, EGF binding was found to decrease as cell density increases. Scatchard analysis of the binding
data reveals that decreases in EGF binding are due to reductions in the number of cell surface EGF receptors. The only apparent
exception is the effect of cell density on the binding of EGF to A-431 cells. For these cells, increases in cell density lead
to two effects: decreases in the number of high affinity EGF receptors and increases in the total number of EGF receptors.
In addition to the effects of cell density on EGF receptors, it was determined that increases in cell density can coordinately
down-regulate receptors for as many as four different growth factors. Overall, the findings described in this report for EGF
and those previously described for transforming growth factor type-β (TGF-β) and fibroblast growth factor (FGF) demonstrate
the existence of a common mechanism for down-regulating growth factor receptors.
This work was supported by grants from the Nebraska Department of Health (89-51), the National Cancer Institute (Laboratory
Research Center Support Grant, CA36727), and the American Cancer Society (Core Grant ACS SIG-16).
EDITOR'S STATEMENT The paper by Rizzino et al. demonstrates that receptor number decreases as a function of cell density.
This may represent a mechanism by which cell proliferation is reduced as cell density increases. 相似文献
95.
Following the successful introduction ofEpidinocarsis lopezi (De Santis) for biological control of the cassava mealybug (CM)Phenacoccus manihoti Mat.-Ferr. in southwestern Nigeria in 1981 and 1982, 11 groups of cassava fields were sampled every 2 weeks up to 1988 for
impact assessment. After 1984, CM populations remained mostly below 10 per tip despite the presence of native hyperparasitoids,
demonstrating the long-term success of biological control byE. lopezi in the region. Indigenous polyphagous coccinellids were found only during peak host densities, whereas the specificE. lopezi was common throughout the year. During some periods, percentage parasitism indicated delayed density dependence. Since 89%
of all sampled cassava tips had no CM at all and the parasitisme is very mobile, parasitization rates were also calculated
for individual infested tips (N=4,878). Parasitism increased slightly with host density on tips having between 1 and 10 CM
of the 3rd and 4th instars, indicating positive density dependence. Such tips comprised 64% of all infested tips. At higher host densities,
parasitism rates fell rapidly. The results are discussed in view of different theories on population regulation by biological
control agents.
相似文献
96.
Changes in foliage density distribution with altitude and its effect on shoot growth and flowering were studied in forest section planes (profiles) of subalpine forests and scrubs (Krummholz) in Nepal and Japan.Patterns of foliage in forest canopy were evaluated by an analysis of variance. Foliage densities were very high at high altitude canopies, but the change in spatial patterns along altitude differs in both areas.The spatial pattern of new shoot production was similar to that of current foliage density and was affected by the amount of foliage above the sample probably through light condition. Flowering of tall trees occurred in the surface of the upper canopy, but a shrub species flowered even under tree canopies. 相似文献
97.
Dominance in demand-feeding behaviour in Arctic charr and rainbow trout: the effect of stocking density 总被引:2,自引:0,他引:2
The effects of stocking density on the demand feeding behaviour of Arctic charr Salvelinus alpinus and rainbow trout Oncorhynchus mykiss were evaluated using demand feeders in combination with a monitoring system based on automatic passive integrated transponders. The proportion of total bites accounted for by top-ranked charr and trout decreased from 87 to 15% and from 66 to 15%, respectively, when moving from the lowest to the highest densities, indicating that the ability of the highest-ranked fish to monopolize the demand feeders was reduced at high densities. However, when the individuals were grouped into quartiles, based on their individual rank, both charr and trout ranked within the upper quartile accounted for the majority of bites independent of stocking density (87 and 72% of the total, respectively). Instead of there being a single dominant individual, as was the case under low density conditions, a small group of individuals dominated the actuation of the trigger at high densities. The two species responded differently to crowding, with the total daily bite activity of charr increasing linearly with increasing density, whereas the corresponding relationship was quadratic for rainbow trout, with a peak value at 24 to 36kg m−3 . 相似文献
98.
Myelin Gene Expression in Immortalized Schwann Cells: Relationship to Cell Density and Proliferation 总被引:1,自引:1,他引:0
Naokazu Sasagasako Kenichi Toda Melissa Hollis Richard H. Quarles 《Journal of neurochemistry》1996,66(4):1432-1439
Abstract: Myelin gene expression was investigated in the immortalized S16 Schwann cell line grown in the presence and absence of serum and at different densities. Protein expression was monitored by western blotting, and message levels were determined by RNase protection assays. To study cell proliferation rates at different cell densities and serum conditions. [3H]thymidine uptake assays and cell counts were performed. Although serum deprivation decreased cell proliferation as expected, the proliferation of S16 cells was unchanged or slightly increased at high density under the conditions of our experiments in either serum-containing or serum-free medium. This increased cell division at high density appeared to be due to greater release of an autocrine growth factor to the medium by dense cell populations. For both sparse and dense cells, substantially more P0 glycoprotein (P0) and myelin-associated glycoprotein (MAG) per milligram of total cellular protein were expressed when the cells were proliferating slowly in defined medium in comparison with more rapidly proliferating cells in serum-containing medium. Furthermore, in both serum-containing and defined media, dense cell populations expressed more MAG and P0 than sparse ones. P0 mRNA and MAG mRNA levels generally paralleled protein levels. The level of mRNA for peripheral myelin protein-22 (PMP-22) was also increased at high cell density but did not change much when proliferation was decreased by serum deprivation. PMP-22 protein was not detected under any of the growth conditions. The changes in expression of these genes with growth conditions may be specific for myelin proteins, because the expression of a nonmyelin glycoprotein, L1, remained constant. The level of cyclic AMP in the cells did not change with the different growth conditions tested. The results indicate that the S16 Schwann cell line mimics primary or secondary Schwann cells by down-regulating myelin gene expression when it proliferates more rapidly in the presence of serum. Furthermore, in both the presence and absence of serum, there was greater expression of myelin genes at high cell density that was not associated with a decreased proliferative rate. Because evidence for a role of secretory factors in affecting myelin gene expression was not obtained by treating sparse S16 cells with medium conditioned by dense S16 cells, the results suggest that the higher expression of myelin genes at high density may be mediated by cell-to-cell contact. 相似文献
99.
Age-specific mortality rates were studied at two adult density levels in four inbred lines of Drosophila melanogaster. In experimental populations, adult densities were maintained at constant levels throughout the experiment by replacing dead flies with live, marked mutants. In control populations, densities declined naturally as the cohorts aged. For all experimental populations the best mortality model is the two-stage Gompertz model, with slower mortality acceleration at older ages. Flies in the experimental populations generally lived longer than flies in control populations, regardless of sex, genotype, or initial density level. The data demonstrate that deceleration of age-specific mortality rates at older ages is not caused by declining cohort densities. Mortality deceleration is a real phenomenon that raises serious questions about the evolution of senescence. 相似文献
100.
Forest density expressing the stocking status constitutes the major stand physiognomic parameter of Indian forest. Density
and age are often taken as surrogate to structural and compositional changes that occur with the forest succession. Satellite
remote sensing spectral response is reported to provide information on structure and composition of forest stands. The various
vegetation indices are also correlated with forest canopy closure. The paper presents a three way crown density model utilizing
the vegetation indices viz., advanced vegetation index, bare soil index and canopy shadow index for classification of forest
crown density. The crop and water classes which could not be delineated by the model were finally masked from normalized difference
vegetation index and TM band 7 respectively. The rule based approach has been implemented for land use and forest density
classification. The broad land cover classification accuracy has been found to be 91.5%. In the higher forest density classes
the classification accuracy ranged between 93 and 95%, whereas in the lower density classes it was found to be between 82
and 85%. 相似文献