Author Keywords: Thermal acclimation; thermal hardening; hardening; heat hardening; cold hardening; critical thermal maxima; critical thermal minima; developmental stage; metamorphosis; tadpoles; Rana catesbeiana 相似文献
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51.
Structural changes in the purified accompanying detergent inactivation were investigated by monitoring changes in light scattering, intrinsic protein fluorescence, and tryptophan to β-parinaric acid fluorescence resonance energy transfer. Two phases of inactivation were observed using the non-ionic detergents, digitonin, Lubrol WX and Triton X-100. The rapid phase involves detergent monomer insertion but little change in protein structure or little displacement of closely associated lipids as judged by intrinsic protein fluorescence and fluorescence resonance energy transfer. Lubrol WX and Triton X-100 also caused membrane fragmentation during the rapid phase. The slower phase of inactivation results in a completely inactive enzyme in a particle of 400 000 daltons with 20 mol/mol of associated phospholipid. Fluorescence changes during the course of the slow phase indicate some dissociation of protein-associated lipids and an accompanying protein conformational change. It is concluded that non-parallel inhibition of and activity by digitonin (which occurs during the rapid phase of inactivation) is unlikely to require a change in the oligomeric state of the enzyme. It is also concluded that at least 20 mol/mol of tightly associated lipid are necessary for either or activity and that the rate-limiting step in the slow inactivation phase involves dissociation of an essential lipid. 相似文献
52.
Kalevi Kurkijrvi Raimo Raunio Timo Korpela 《International journal of biological macromolecules》1981,3(6):389-394
Electron absorption and equilibrium of the Schiffs bases prepared between pyridoxal 5′-phosphate (PLP) and dodecylamine (DODA) or some other shorter chain amines have been studied in nonionic and cationic micellar solutions with various pH of the bulk solution. In the presence of the nonionic (Triton X-100) micelles the Schiffs bases formed between PLP and DODA were embedded into the micelles because the absorption occured at 335 nm, indicative of the nonpolar milieu. This absorption was constant at pH 5–10. At pH 3–5, the tautomeric form absorbing at 415 nm appeared. This resembles the titration of glycogen phosphorylate or that of Schiffs bases in methanol. Short chain amines absorbed at 415 nm, which is typical of Schiffs bases in aqueous solutions. Tryptophan also absorbed first at 415 nm but the absorption changed to 325 nm with a half-time of ~20 min. This was interpreted as being due to formation of the cyclic structure catalysed by micelles. The pH-dependent equilibrium constant of the reaction between PLP and DODA in Triton X-100 solution had a maximum at pH9, the value being 3500 M?1, about ten times greater than the value of ethylamine at the same pH. Spectral properties of PLP-DODA imines in the cationic micelles (cetyltrimethylammonium bromide) resembled those in the nonionic micelles, except that at low pH the absorption peak in the 415 nm region did not appear. The equilibrium constant of PLP-DODA had maximum at pH 9, the value being as high as 118000 M?1. Different properties of nonionic and cationic micelles and the design of micellar model systems of PLP enzymes are discussed. 相似文献
53.
The effect of four prediapause temperatures (18, 22, 26 and 30°C) on the photoperiodic response of the codling moth, Cydia pomonella (L.), was studied under controlled conditions. The highest rates of diapause were recorded, for all day-lengths, at temperatures of 22 and 26°C while relatively lower rates of diapause were elicited at 18 and 30°C. The same trend was demonstrated by projecting the values of the critical photoperiod which induces 50% diapause (=CPhP50) over the prediapause temperature. The change in diapause incidence as a function of photoperiod, at all prediapause temperatures, exhibited a response characteristic of long-day insects, i.e. high rates of diapause at short days (12–13.5 h) and a decrease in diapause incidence at long days (14–15 h). The results for temperatures 22, 26 and 30°C support the view that lower prediapause temperatures enhance diapause induction, at a give photoperiod, while higher temperatures tend to avert or diminish the process. On the other hand, the low rates of diapause obtained at 18°C contradict this view. Nevertheless, high correlation was found between the laboratory evidence and field data, indicating the adaptability of the Israeli codling moth to subtropical climate. 相似文献
54.
55.
I. G. Burns 《Plant and Soil》1992,142(2):221-233
A method is described for determining the way in which growth rate varies with plant nutrient concentration using a simple
nutrient interruption technique incorporating only 2 treatments. The method involves measuring the changes in growth and nutrient
composition of otherwise well-nourished plants after the supply of one particular nutrient has been withheld. Critical concentrations
are estimated from the relationship between the growth rate (expressed as a fraction of that for control plants of the same
size which remained well-nourished throughout) and the concentration of the growth-limiting nutrient in the plants as deficiency
developed. Trials of the method using young lettuce plants showed that shoot growth rate was directly proportional to total
N (nitrate plus organic N) concentration, and linearly or near-linearly related to K and P concentration over a wide range;
the corresponding relationship for nitrate was strongly curvi-linear. Critical concentrations (corresponding to a 10% reduction
in growth rate) determined from these results were similar to critical values calculated from models derived from field data,
but were generally higher than published estimates of critical concentration (based on reductions in shoot weight) for plants
of a similar size. Reasons for these discrepancies are discussed. Nitrate, phosphate or potassium concentrations in sap from
individual leaf petioles were highly sensitive to changes in shoot growth rate as deficiency developed, with the slope of
the relationships varying with leaf position, due to differences both in their initial concentration and in the rates at which
they were utilized in individual leaves. Each nutrient was always depleted more quickly in younger leaves than in older ones,
providing earlier evidence of deficiency for diagnostic purposes. Although the plants were capable of accumulating nitrate,
phosphate and potassium well in excess of that needed for optimum dry matter production during periods of adequate supply,
the rate of mobilization of these reserves was insufficient to prevent reductions in growth rate as the plants became deficient.
This brings into question the validity of the conventional concept that luxury consumption provides a store of nutrients which
are freely available for use in times of shortage. The implications of these results for the use of plant analysis for assessing
plant nutrient status are discussed. 相似文献
56.
J. P. R. Ochieng'-Odero 《Entomologia Experimentalis et Applicata》1990,54(1):21-27
Adult reproductive performance is linked to the period of feeding done by the final instar larva after attainment of a larval critical weight (LCW). The highest weight attained by a final instar larva is referred to as the larval maximum weight (LMW) and is the onset of the pre-pupal period. The relationships between LCW, pupal weight (PW) and adult weight (AW) are described as functions of the LMW. In the leafroller Cnephasia jactatana (Walker) (Lepidoptera: Tortricidae) LCW was dependent on larval size and was approximately 75% of the mean LMW. LCW was about 29 mg and 36 mg for male and female larvae of 1.18 mm and 1.20 mm head-capsule width, respectively. Over three successive generations of laboratory rearing, PW was approximately 30% and 25% lower than the LMW for males and females, respectively. AW was consistently about 50% and 40% lower than the PW for males and females, respectively. The decrease in weight from LMW to PW was named as the constant DP and found to be 0.3 for males and 0.25 for females. The total decrease from LMW to AW was the constant DA and was 0.6 for males and 0.5 for females. The duration of the latent feeding period was positively correlated to PW and AW. LCW may be used to derive quality indices that describe and predict pupal and adult performance.
Résumé Les performances reproductives sont liées à la période d'alimentation du dernier stade larvaire après l'obtention d'un poids critique (LCW). Le poids maximal atteint au dernier stade larvaire est défini comme le poids larvaire maximal (LMW), il correspond au début de la période prénymphale. Les relations entre poids de la chrysalide (PW) et poids de l'audulte AW) sont présentées comme des fonctions de LMW). Chez Cnephasia jactatana Walker (Lep. Tortricidae), LCW dépend de la taille de la chenille et correspond à environ 75% de la valeur moyenne de LMW. LCW est respectivement de 29 mg et 36 mg pour les chenilles mâles et femelles dont les capsules céphaliques ont 1,18 et 1,20 mm. Pour 3 générations successives, PW est environ 30% et 25% inférieur à LMW des mâles et des femelles. La régression du poids de LMW à PW est désignée comme la constante DP et vaut 0,3 pour les mâles et 0,25 pour les femelles. La régression de LMW à AW est désignée comme la régression DM et vaut 0,6 pour les mâles et 0,5 pour les femelles. La durée de la période d'alimentation latente est liée positivement à PW et AW. LCW peut être utilisé comme indice dérivé décrivant et prédisant les potentialités nymphales et imaginales.相似文献
57.
1. 1.|Critical thermal maxima (CTMax) and minima (CTMin) were measured to evalute thermal hardening in Rana catesbeiana.
2. 2.|Tadpoles show heat hardening and CTMax acclimation, and both responses are influenced by developmental stage.
3. 3.|The first evidence of cold hardening in vertebrates is reported here.
4. 4.|Heat hardening significantly reduces cold tolerance, but there is otherwise no evidence of a cross-hardening effect.
58.
Summary Studies on the survival ofPseudomonas solanacearum, the causal agent of bacterial wilt of tomato, under laboratory conditions showed that soil amendments had little effect
on the population of the pathogen. When the host plant was grown in amended soil there was a positive influence on growth
of the pathogen. The population level of the pathogen incorporated into the soil was reduced to one-half within a period of
46 days.
The pathogen survived in the rhizosphere of non-host plants belonging to the families Acanthaceae and Leguminosae even in
the absence of the natural hosts, but its incidence in the rhizosphere of plants belonging to Graminae and Cyperaceae was
comparatively low indicating possibilities of reducing the inoculum potential of the pathogen in tomato fields by allowing
such plants to grow during fallow periods.
Part of the thesis of the Senior Author presented to the Kerala Agricultural University, Trichur, Kerala State, India. 相似文献
59.
K. S. Chung 《Hydrobiologia》1981,78(2):177-181
The acclimation rates of temperature changes in Cyprinodon dearborni, collected from Laguna Los Patos, Cumana, Venezuela, were determined by the critical thermal maximum method.
At an increase in temperature (from 24 to 31°C) fish started to gain acclimation level after 3 hours and took 3 days to fully
get up to a higher level of resistance to heat death; however, at a decrease in temperature (from 3 t to 24°C) fish began
to lose its acclimation level after 12 days and required 39 days to reach a lower level of resistance to thermal death. 相似文献
60.
Chinese hamster ovary cells, selected in mitosis and plated into medium containing hydroxyurea, can progress through G1 and enter S phase although bulk DNA synthesis is prevented. As the cells progress through G1 in the presence of hydroxyurea, ornithine decarboxylase activity remains low while general protein synthesis appears unaffected. After hydroxyurea is removed, ornithine decarboxylase activity increases, but only after approximately 20% of the DNA has been replicated. These results suggest that ornithine decarboxylase induction is not essential for cellular progression into S phase but is required for the completion of DNA synthesis. 相似文献