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51.
The invasion of woody plants into grass‐dominated ecosystems has occurred worldwide during the past century with potentially significant impacts on soil organic carbon (SOC) storage, ecosystem carbon sequestration and global climate warming. To date, most studies of tree and shrub encroachment impacts on SOC have been conducted at small scales and results are equivocal. To quantify the effects of woody plant proliferation on SOC at broad spatial scales and to potentially resolve inconsistencies reported from studies conducted at fine spatial scales, information regarding spatial variability and uncertainty of SOC is essential. We used sequential indicator simulation (SIS) to quantify spatial uncertainty of SOC in a grassland undergoing shrub encroachment in the Southern Great Plains, USA. Results showed that both SOC pool size and its spatial uncertainty increased with the development of woody communities in grasslands. Higher uncertainty of SOC in new shrub‐dominated communities may be the result of their relatively recent development, their more complex above‐ and belowground architecture, stronger within‐community gradients, and a greater degree of faunal disturbance. Simulations of alternative sampling designs demonstrated the effects of spatial uncertainty on the accuracy of SOC estimates and enabled us to evaluate the efficiency of sampling strategies aimed at quantifying landscape‐scale SOC pools. An approach combining stratified random sampling with unequal point densities and transect sampling of landscape elements exhibiting strong internal gradients yielded the best estimates. Complete random sampling was less effective and required much higher sampling densities. Results provide novel insights into spatial uncertainty of SOC and its effects on estimates of carbon sequestration in terrestrial ecosystem and suggest effective protocol for the estimating of soil attributes in landscapes with complex vegetation patterns. 相似文献
52.
Shi Y Shen B Xiang P Yan H Shen M 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2010,878(30):3161-3166
Ethyl glucuronide (EtG) has been shown to be a suitable marker of excessive alcohol consumption. Determination of EtG in hair samples may help to differentiate social drinkers from alcoholics, and this testing can be widely used in forensic science, treatment programs, workplaces, military bases as well as driving ability test to provide legal proof of drinking. A method for determination of EtG in hair samples using large volume injection-gas chromatography-tandem mass spectrometry (LVI-GC/MS/MS) was developed and validated. Hair samples (in 1 mL deionized water) were ultrasonicated for 1h and incubated overnight; these samples were then deproteinated to remove impurities and derivatisated with 15 μL of pyridine and 30 μL of BSTFA. EtG was detected using GC/MS/MS in multiple-reaction monitoring mode. This method exhibited good linearity: y=0.0036 x+0.0437, R2=0.9993, the limit of detection and the limit of quantification were 5 pg/mg and 10 pg/mg, respectively. The extraction recoveries were more than 60%, and the inter-day and intra-day relative standard deviations (RSD) were less than 15%. This method has been applied to the analysis of EtG in hair samples from 21 Chinese subjects. The results for samples obtained from all of those who were teetotallers were negative, and the results for the other 15 samples ranged from 10 to 78 pg/mg, except for one negative sample. These data are the basis for interpretation of alcohol abuse. 相似文献
53.
The light-driven photocycle of rhodopsin begins the photoreceptor cascade that underlies visual response. In a sequence of events, the retinal covalently attached to the rhodopsin protein undergoes a conformational change that communicates local changes to a global conformational change throughout the whole protein. In turn, the large-scale protein change then activates G-proteins and signal amplification throughout the cell. The nature of this change, involving a coupling between a local process and larger changes throughout the protein, may be important for many membrane proteins. In addition, functional work has shown that this coupling occurs with different efficiency in different lipid settings. To begin to understand the nature of the efficiency of this coupling in different lipid settings, we present a molecular dynamics study of rhodopsin in an explicit dioleoyl-phosphatidylcholine bilayer. Our system was simulated for 40 ns and provides insights into the very early events of the visual cascade, before the full transition and activation have occurred. In particular, we see an event near 10 ns that begins with a change in hydrogen bonding near the retinal and that leads through a series of coupled changes to a shift in helical tilt. This type of event, though rare on the molecular dynamics time-scale, could be an important clue to the types of coupling that occur between local and large-scale conformational change in many membrane proteins. 相似文献
54.
基于右手螺旋短杆菌肽A离子通道模型,利用分子动力学计算机模拟方法研究了通道内离子K+,Na+,Li+与水分子的相关性. 相似文献
55.
56.
对河西走廊荒漠植物的群落类型、盖度、密度、重要值、地上生物量等进行了调查,收集整理各区域的气候资料,对它们的关系进行了初步研究。结果显示:(1)物种种类组成、总密度与海拔、经度呈正相关,与纬度呈负相关,在走廊的东南→西北方向上呈递减趋势。(2)在活动积温(>10℃)>3 200℃的荒漠地区,灌木(包括半灌木)在群落中占有绝对优势,其重要值可达到100;当活动积温在3 000℃左右时,群落往往是一些过渡类型,或以草本为优势种、以半灌木或灌木为伴生种,或以半灌木为优势种、以多年生草本为伴生种。(3)在年降水量110 mm以上的荒漠地区,植物群落生活型主要为半灌木或多年生草本;在年降水量110 mm以下的地区,生活型主要为小灌木或一年生草本;在年降水量小于50 mm的极端干旱地区,只有在雨季时有一年生草本生长。表明荒漠生态系统植被稀疏,生物量小,对气候因子,特别是降水变化敏感,对其保育显得尤为重要。 相似文献
57.
Molecular dynamics simulations have become a popular and powerful technique to study lipids and membrane proteins. We present some general questions and issues that should be considered prior to embarking on molecular dynamics simulation studies of membrane proteins and review common simulation methods. We suggest a practical approach to setting up and running simulations of membrane proteins, and introduce two new (related) methods to embed a protein in a lipid bilayer. Both methods rely on placing lipids and the protein(s) on a widely spaced grid and then 'shrinking' the grid until the bilayer with the protein has the desired density, with lipids neatly packed around the protein. When starting from a grid based on a single lipid structure, or several potentially different lipid structures (method 1), the bilayer will start well-packed but requires more equilibration. When starting from a pre-equilibrated bilayer, either pure or mixed, most of the structure of the bilayer stays intact, reducing equilibration time (method 2). The main advantages of these methods are that they minimize equilibration time and can be almost completely automated, nearly eliminating one time consuming step in MD simulations of membrane proteins. 相似文献
58.
Haiyan Luo Sanghun Sin Joseph M. McDonough Carmen R. Isasi Raanan Arens David M. Wootton 《Journal of biomechanics》2014
Background
Improvements in obstructive sleep apnea syndrome (OSAS) severity may be associated with improved pharyngeal fluid mechanics following adenotonsillectomy (AT). The study objective is to use image-based computational fluid dynamics (CFD) to model changes in pharyngeal pressures after AT, in obese children with OSAS and adenotonsillar hypertrophy.Methods
Three-dimensional models of the upper airway from nares to trachea, before and after AT, were derived from magnetic resonance images obtained during wakefulness, in a cohort of 10 obese children with OSAS. Velocity, pressure, and turbulence fields during peak tidal inspiratory flow were computed using commercial software. CFD endpoints were correlated with polysomnography endpoints before and after AT using Spearman?s rank correlation (rs).Results
Apnea hypopnea index (AHI) decreases after AT was strongly correlated with reduction in maximum pressure drop (dPTAmax) in the region where tonsils and adenoid constrict the pharynx (rs=0.78, P=0.011), and with decrease of the ratio of dPTAmax to flow rate (rs=0.82, P=0.006). Correlations of AHI decrease to anatomy, negative pressure in the overlap region (including nasal flow resistance), or pressure drop through the entire pharynx, were not significant. In a subgroup of subjects with more than 10% improvement in AHI, correlations between flow variables and AHI decrease were stronger than in all subjects.Conclusions
The correlation between change in dPTAmax and improved AHI suggests that dPTAmax may be a useful index for internal airway loading due to anatomical narrowing, and may be better correlated with AHI than direct airway anatomic measurements. 相似文献59.
基于信息熵的我国日降水量随机性和时空差异性 总被引:1,自引:0,他引:1
我国日降水过程呈现明显的随机性与时空差异性,如何准确认识其时空变化规律对洪涝灾害防治等实际工作的影响具有重要意义.本文基于1961—2013年全国520个气象站点的日降水数据,选用信息熵指标研究我国日降水量的随机性.结果表明:研究期间,我国东南地区日降水量的随机性大于西北地区,且不同等级日降水量随机性的空间分布存在差异,小雨(降雨量0.1~10 mm,P_0)等级日降水量随机性较大,差异不明显,中雨(10~25 mm,P10)、大雨(25~50 mm,P25)等级日降水量随机性最大,差异明显,暴雨及以上(≥50 mm,P50)等级日降水量随机性最小,差异最明显.整体上,日降水的信息熵值呈上升趋势,表明全球气候变化下我国大部分地区日降水量的随机性增大,尤其表现为极端暴雨发生的频次明显增大.日降水信息熵的空间分布及其变化趋势可以很好地综合反映我国日降水量随机性的空间分布格局,可为洪涝灾害防治、农业规划布局、生态环境规划等提供科学依据. 相似文献
60.
Reetta Piskonen Anu Kapanen Timo Mansikka Jorma Rytk nen Merja It vaara 《Bioremediation Journal》2002,6(2):143-158
A microcosm test was designed to study the efficiency of bioremediation treatments at oil contaminated shorelines. The biodegradation in the hermetically closed microcosm was monitored by measuring the total cumulative inorganic carbon evolved during the bioremediation process. The effects of three different additives, medium-release methylene urea (MU) + apatite, fast-release MU + superphosphate, and a biosorbent, on the biodegradation of weathered crude oil (North Sea Brent) were evaluated at +10°C. All the additives significantly increased mineralization. The total amount of inorganic carbon evolved during the 10-week study was measured in the microcosm treated with oil, and with oil and medium-release MU + apatite, fast-release MU + superphosphate, and biosorbent. The amounts were 40,670,490, and 580 mg, respectively. The respirometric measurements were supported by microbiological determinations, ATP content in the sand, number of heterotrophic bacteria, and amount of biomass-C determined by the substrate-induced respiration method. Nutrient analysis indicated that biodegradation was nitrogen limited. The microcosm test proved to be suitable for comparing the effectiveness of different treatments in enhancing the biodegradation of crude oil-contaminated shores. 相似文献