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151.
152.
Diurnal variations of amino acids and organic acids in xylem fluid from Lagerstroemia indica: an endogenous circadian rhythm 总被引:2,自引:0,他引:2
Diurnal variations in the concentrations of major organic compounds occurred in xylem fluid extracted from Lagerstroemia indica L. The concentration of amino acids and the N/C ratio was at a maximum and that of organic acids was at a minimum between 1230 and 2030 h. Since the concentrations of total organic nitrogen, total amino acids and most individual amino acids (but not organic acids or sugars) were also proportional to xylem tension two experiments were performed to discern whether variations in chemistry were a consequence of diurnal changes in moisture stress. In the first experiment, L. indica , exposed to variable levels of moisture stress during midday, manifested an increase in organic acids and a reduction in the N/C ratio. In the second experiment, chemical profiles of xylem fluid were collected and compared for plants exposed to a natural photoperiod, constant darkness or continuous light at noon and midnight. After 1 day amino acids increased in concentration during midday for all treatments; the variation was greatest (10-fold) for plants in constant darkness where xylem tension varied from 0.20 to 0.25 MPa. Only plants exposed to continuous light lost a diurnal rhythm after 3 days. Thus, the circadian rhythm was endogenous, terminated in continuous light and was not mediated by changes in moisture stress. Glutamine accounted for most of the diurnal variation in total amino acids, organic nitrogen and the N/C ratio in xylem fluid. 相似文献
153.
Mobilization of different phosphate fractions in the rhizosphere 总被引:6,自引:1,他引:5
Availability of soil P fractions and mechanisms of acquisition by plants were studied. Plants mobilize soil P by desorption via depletion of P solution concentration around roots. In an oxisol, the process was enhanced by nitrate N nutrition of ryegrass, which increased soil pH, and by carboxylate release by white lupin. Ligand exchange and Fe/Al solubilization are assumed to be the mechanisms. Ammonium N nutrition of ryegrass decreased pH and allowed P mobilization in a luvisol but had no such effect in an oxisol, due to acid solubility of P in these soils. Organic P dissolved in soil solution contributed one third to the P uptake of field-grown barley on a luvisol. Laboratory experiments suggest that organic P is hydrolyzed by phosphatases at the root surface and replenished by micro-organisms. 相似文献
154.
Refixation of xylem sap CO2 in Populus deltoides 总被引:1,自引:0,他引:1
Vascular plants have respiring tissues which are perfused by the transpiration stream, allowing solubilization of respiratory CO2 in the xylem sap. The transpiration stream could provide a conduit for the internal delivery of respiratory CO2 to leaves. Trees have large amounts of respiring tissues in the root systems and stems, and may have elevated levels of CO2 in the xylem sap which could be delivered to and refixed by the leaves. Xylem sap from the shoots of three Populus deltoides trees had mean dissolved inorganic carbon concentrations (CO2+H2CO3+HCO?3) ranging from 0. 5 to 0. 9 mM. When excised leaves were allowed to transpire 1 mM[14C]NaHCO3, 99. 6% of the label was fixed in the light. Seventy-seven percent of the label was fixed in major veins and the remainder was fixed in the minor veins. Autoradiography confirmed that label was confined to the vasculature. In the dark, approximately 80% of the transpired label escaped the leaf, the remainder was fixed in the major veins, slightly elevating dark respiration measurements. This indicates that the vascular tissue in P. deltoides leaves is supplied with a carbon source distinct from the atmospheric source fixed by interveinal lamina. However, the contribution of CO2 delivered to the leaves in the transpiration stream and fixed in the veins was only 0. 5% of atmospheric CO2 uptake. In the light 90% of the label was found in sugar, starch and protein, a pattern similar to that found for atmospheric uptake of[14C]CO2. Compared with leaves labelled in the light, leaves labelled in the dark had more label in organic acid, amino acid and protein and less label in sugar and starch. After a 5-s pulse the majority of the label fed to petioles in both the light and the dark was found in malate. The majority of the label was found in malate at 120 s in the dark; only 2% of the label was found in phosphorylated compounds at 120 s. The proportion of label found in phosphorylated compounds increased from 17% at 5 s to 80% at 120 s in the light. This suggests that CO2 delivered to leaves in the light via the transpiration stream is fixed in the veins, a small portion through dark fixation into malate, the remainder by C-3 photosynthesis. 相似文献
155.
S. Abarzua R. Altenburger R. Callies L.-H. Grimme A. Mayer D. Leibfritz U. Schiewer 《Physiologia plantarum》1993,89(3):659-663
Over a period of several days, rhythmic changes in extracellular NH+ 4 concentration take place in cultures of the cyanobacterium Microcystis firma (Bré et Lenorm.) Schmidle, strain Gromov/St. Petersb. 398, under conditions of restricted CO2 supply and light/dark alternation. The changes are enhanced by nitrate supply. Among the various processes generating intracellular NH+ 4 (NH4 4 uptake, NO− 3 reduction, protein and amino acid degradation, photorespiration), NO− 3 reduction appears as the one most important. This can be concluded from experiments with and without nitrate and/or ammonium in the medium. In the presence of saturating CO2 , continuous light, or continuous darkness, rhythmic NH+4 4 oscillations are not induced. Studies of the incorporation of NH+ 4 nitrogen by in vivo 15 N-NMR show that if CO2 is supplied, 15 N is accumulated in several components with the following time course: in the first hour in Gln (δ), in the second hour in the α-amino groups of most nonbranched amino acids, in the third hour in γ-aminobutyric acid (GABA), Orn (δ) and Lys (ε), and in the sixth hour in Ala. Carbon limitation, however, results in accumulation of label in the amide nitrogen of glutamine only. 相似文献
156.
Estimating regional carbon stocks and spatially covarying edaphic factors using soil maps at three scales 总被引:2,自引:1,他引:1
Most estimates of regional and global soil carbon stocks are based on extrapolations of mean soil C contents for broad categories
of soil or vegetation types. Uncertainties exist in both the estimates of mean soil C contents and the area over which each
mean should be extrapolated. Geographic information systems now permit spatially referenced estimates of soil C at finer scales
of resolution than were previously practical. We compared estimates of total soil C stocks of the state of Maine using three
methods: (1) multiplying the area of the state by published means of soil C for temperate forests and for Spodosols; (2) calculating
areas of inclusions of soil taxa in the 1:5,000,000 FAO/UNESCO Soils Map of the World and multiplying those areas by selected
mean carbon contents; and (3) calculating soil C for each soil series and map unit in the 1:250,000 State Soil Geographic
Data Base (STATSGO) and summing these estimates for the entire state. The STATSGO estimate of total soil C was between 23%
and 49% higher than the common coarse scale extrapolations, primarily because STATSGO included data on Histosols, which cover
less than 5% of the area of the state, but which constitute over one-third of the soil C. Spodosols cover about 65% of the
state, but contribute less than 39% of the soil C. Estimates of total soil C in Maine based on the FAO map agreed within 8%
of the STATSGO estimate for one possible matching of FAO soil taxa with data on soil C, but another plausible matching overestimated
soil C stocks. We also compared estimates from the 1:250,000 STATSGO database and from the 1:20,000 Soil Survey Geographic
Data Base (SSURGO) for a 7.5 minute quadrangle within the state. SSURGO indicated 13% less total soil C than did STATSGO,
largely because the attribute data on depths of soil horizons in SSURGO are more specific for this locality. Despite localized
differences, the STATSGO database offers promise of scaling up county soil survey data to regional scales because it includes
attribute data and estimates of areal coverage of C-rich inclusions within map units. The spatially referenced data also permit
examination of covariation of soil C stocks with soil properties thought to affect stabilization of soil C. Clay content was
a poor predictor of soil C in Maine, but drainage class covaried significantly with soil C across the state. 相似文献
157.
Field measures of tree and shrub dimensions were used with established biomass equations in a stratified, two-stage cluster sampling design to estimate above-ground ovendry woody biomass and carbon storage of the eastern deciduous forest of North America. Biomass averaged 8.1 ± 1.4 (95% C.I.) kg/m2 and totaled 18.1 ± 3.1 (95% C.I.) gigatons. Carbon storage averaged 3.6 ± 0.6 (95% C.I.) kg/m2 and totaled 8.1 ± 1.4 (95% C.I.) gigatons. These values are lower than previous estimates commonly used in the analysis of the global carbon budget which range from 17.1 to 23.1 kg/m2 for biomass and 7.7 to 10.4 kg/m2 for carbon storage. These new estimates for the deciduous forest, together with earlier work in the boreal forest begin to reveal a pattern of overestimation of global carbon storage by vegetation in analyses of the global carbon budget. We discuss reasons for the differences between the new and earlier estimates, as well as implications for our understanding of the global carbon cycle. 相似文献
158.
Nitrate limitation of N2O production and denitrification from tropical pasture and rain forest soils
William F. J. Parsons Martin E. Mitre Michael Keller William A. Reiners 《Biogeochemistry》1993,22(3):179-193
Nitrous oxide production was measured in intact cores taken from active pasture and old-growth forest Inceptisols in the Atlantic Lowlands of Costa Rica. Following additions of aqueous KNO3 or glucose, or the two combined amendments, the cores were incubated in the laboratory to determine if N2O production rates were either N-limited or C-limited in the two land use types. Differences in rates of denitrification (N22O + N2 production) among amended forest and pasture soils were determined by addition of 10% C2H2.The forest soils were relatively insensitive to all amendment additions, including the acetylene block. Forest N2O production rates among the treatments did not differ from the controls, and were consistently lower than those of the pasture soils. With the addition of glucose plus nitrate to the forest soils, production of N2O was three times greater than the controls, although this increase was not statistically significant. On the other hand, the pasture soils were definitely nitrogen-limited since N2O production rates were increased substantially beyond controls by all the amendments which contained nitrate, despite the very low N level (5 mg N kg–1 soil) relative to typical fertilizer applications. With respect to the nitrate plus glucose plus acetylene treatment, denitrification was high in the pasture soils; N2O production in the presence of C2H2 was 150% of the rate of N2O production measured in the absence of the acetylene block. The results are discussed in relation to the effects of agricultural land use practices and subsequent impacts of disturbance on N2O release. 相似文献
159.
The extent to which the parasitic angiosperm Striga hermonthica reduces the growth of its sorghum host is dependent on the concentration of nitrogen (as NH4NO3 in 40% Long Ashton Solution) supplied to the plants. The biomass of 0.5,1 and 2 mol m?3 N-grown infected plants was 22,30 and 66%, respectively, of uninfected plants after 140d growth. The biomass of 3 and 4 mol m?3 N-grown infected plants differed little from uninfected plants. No grain was set in 0.5 and 1 mol m?3 N-grown infected plants, grain yield reached 42 and 73% of controls in 2 and 3 mol m?3 N-grown plants, and was unaffected in 4 mol m?3 N-grown plants. Striga hermonthica also altered the allometry and architecture of the host, at all but the highest N concentration. Higher N concentration (3 and 4 mol m ?3 N) reduced the growth of S. hermonthica. Foliar N concentrations in sorghum ranged from 11 mg g?1 dwt. in 0.5 mol m?3 N-grown plants, to 28 mg g?1 dwt. in 4 mol m?3 N-grown plants, and were not affected by S. hermonthica. Higher N concentrations were measured in S. hermonthica, and ranged from 18 to 45 mg g?1 dwt. in 0.5 and 3 mol m?3 N-grown plants, respectively. The relationship between photosynthesis (CO2 flux) and N concentration differed between uninfected and infected sorghum. This was most apparent in 0.5 mol m?3 N-grown plants, with rates of 16 and 11 μmol m?2 s?1 in uninfected and infected plants, respectively (at 1500–1800 μmol m?2 s?1 photosynthetic photon flux density). At higher N concentrations, this difference was smaller, with both sets of plants reaching 26 μmol m?2 s?1 at 4 mol m?3 N. Varying the level of S. hermonthica infection showed that the effect of N on host photosynthesis cannot be explained by differences in the mass or number of parasites supported by the host. At low levels of infection in 1 mol m?3 N-grown plants, the negative effect of the parasite was reversed, and photosynthesis in infected plants exceeded that in uninfected plants by 20%. Photosynthesis in S. hermonthica at 3 mol m?3 N (8 μmol m?2 s?1) was double that in 0.5 mol m?3 N-grown plants. Stable carbon isotope and gas exchange measurements data demonstrated that this higher level of autotrophic carbon fixation was accompanied by a lower dependency on hetero trophic carbon. The latter ranged from 27 to 6% in 0 5 mol m?3 and 3 mol m?3 N-grown plants, respectively. 相似文献
160.
Enhanced ethylene production by primary roots of Zea mays L. in response to sub-ambient partial pressures of oxygen 总被引:1,自引:0,他引:1
R. W. BRAILSFORD L. A. C. J. VOESENEK C. W. P. M. BLOM A. R. SMITH M. A. HALL M. B. JACKSON 《Plant, cell & environment》1993,16(9):1071-1080
Ethylene production by primary roots of 72–h-old intact seedlings of Zea mays L. cv. LG11 was studied under ambient and sub-ambient oxygen partial pressures (pO2) using a gas flow-through system linked to a photoacoustic laser detector. Despite precautions to minimize physical perturbation to seedlings while setting-up, ethylene production in air was faster during the first 6h than later, in association with a small temporary swelling of the roots. When roots were switched from air (20–8kPa O2) to 3 or 5kPa O2 after 6h, ethylene production increased within 2—3 h. When, the roots were returned to air 16 h later, ethylene production decreased within 2—3 h. The presence of 10kPa CO2 did not interfere with the effect of 3kPa O2. Transferring roots from air to 12–5kPa did not change ethylene production, while a reduction to 1 kPa O2 induced a small increase. The extra ethylene formed in 3 and 5 kPa O2 was associated with plagiotropism, swelling, root hair production, and after 72 h, increased amounts of intercellular space (aerenchyma) in the root cortex. Root extension was also slowed down, but the pattern of response to oxygen shortage did not always match that of ethylene production. On return to air, subsequent growth patterns became normal within a few hours. In the complete absence of oxygen, no ethylene production was detected, even when anaerobic roots were returned to air after 16 h. 相似文献