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51.
本文主要观察硫酸铝钾——饮用水净化剂引致小鼠肝酶组化,超微结构和组织结构的变化。动物30只,分为正常组、硫酸铝钾大、小剂量组。实验结果:硫酸铝钾10mg/kg/日(大剂量组)与5mg/kg/日(小剂量组)动物用药10天、80天均可使肝SDH酶活性降低;肝细胞线粒体肿胀、嵴断裂和溶解。在光学显微镜下用药80天后大部分肝细胞肿胀,胞浆疏松淡染、肝小叶内可见呈小灶状分布的炎性细胞浸润,主要为淋巴细胞及浆细胞,门管区偶见少许炎性细胞,但肝小叶和门管区未见结缔组织增生。为此,硫酸铝钾作为净水剂,不宜用量过大。 相似文献
52.
The protective effect of arbutin against potassium bromate-induced oxidative damage in the rat brain
H. Turan Akkoyun Ahmet Uyar Mahire Bayramoglu Akkoyun Aydın Şükrü Bengü Şule Melek Fatma Karagözoğlu Sevinç Aydın Suat Ekin Sinem Aslan Erdem 《Journal of biochemical and molecular toxicology》2023,37(2):e23248
This study aimed to investigate the protective effects of arbutin (ARB) against brain injury induced in rats with potassium bromate (KBrO3). The rats were divided into four groups as Group 1: Control (0.9% NaCl ml/kg/day p.), Group 2: KBrO3 (100 mg/kg (gavage), Group 3: ARB (50 mg/kg/day p.), and Group 4: KBrO3 + ARB (100 mg/kg (gavage) + 50 mg/kg/day p.). At the end of the fifth day of the study, the rats in all groups were killed, and their brain tissues were collected. In the collected brain tissues, malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) levels were measured, and routine histopathological examinations were made. The MDA levels in the group that was exposed to KBrO3 were significantly higher than those in the control group (p ˂ 0.001). In comparison to the KBrO3 group, the MDA levels in the KBrO3 + ARB group were significantly lower (p ˂ 0.001). It was observed that SOD and CAT enzyme activity levels were significantly lower in the KBrO3 group compared to the control group (p ˂ 0.001), while these levels were significantly higher in the KBrO3 + ARB group than in the KBrO3 group (p ˂ 0.001). Additionally, the group that was subjected to KBrO3 toxicity, as well as ARB administration, had much lower levels of histopathologic signs than the group that was subjected to KBrO3 toxicity only. Consequently, it was found that KBrO3 exposure led to injury in the brain tissues of the rats, and using ARB was effective in preventing this injury. 相似文献
53.
Z. Lu R. R. Fay 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1993,173(1):33-48
Single units of the goldfish torus semicircularis (TS) were recorded in response to pure tones. Response areas (RA) were obtained by recording the number of spikes evoked by tones in a range of frequencies and levels within the units' dynamic range. RAs gave estimates of best sensitivity (BS), characteristic frequency (CF), most excitatory frequency at each level (BF), and Q10dB. Peri-stimulus-time histograms (PSTH), interspike interval histograms (ISIH), and period histograms were obtained at various frequencies and levels to describe the units' temporal response patterns.The distribution of CF is nonuniform with modes at 155, 455, and 855 Hz. The distribution of the coefficient of synchronization to standard tones is also nonuniform, revealing a dichotomy between units with little or no phase-locking and those that phase-lock strongly. PSTHs for units without significant phase-locking vary widely and include patterns resembling those of the mammalian auditory brainstem. Compared with saccular afferents, torus units tend to have lower spontaneous rates, greater sensitivity, and sharper tuning. Unlike saccular afferents, BF is independent of level for most torus units. Some torus units are similar to saccular afferents while others reveal significant transformations of information between the periphery and the midbrain.Abbreviations BF
best frequency
- BS
best sensitivity
- CF
characteristic frequency
- ISIH
inter-spike interval histogram
- PSTH
peri-stimulus-time histogram
- RA
response area
- TS
torus semicircularis 相似文献
54.
《Current biology : CB》2023,33(16):3478-3488.e3
55.
The effects of cadmium and lead on the internal concentrations of Ca2+ and K+, as well as on the uptake and translocation of K(86Rb+) were studied in winter wheat (Triticum aestivum L. a. MV-8) grown hydroponically at 2 levels of K+ (100 uM and 10 mM). Cd2+ and Pb2+ were applied in the nutrient solution in the range of 0.3 to 1000 u.M. Growth was more severely inhibited by Cd2+ and in the high-K+ plants as compared to Pbz+ and low-K+ plants. Ions of both heavy metals accumulated in the roots and shoots, but the K+ status influenced their levels. Ca2+ accumulation was increased by low concentrations of Cd2+ mainly in low-K+ shoots, whereas it was less influenced by Pb2+. The distribution of Cd2+ and Ca2+ in the plant and in the growth media indicated high selectivity for Cd2+ in the root uptake, while Ca2+ was preferred in the radial and/or xylem transport. Cd2+ strongly inhibited net K+ accumulation in high-K+ plants but caused stimulation at low K+ supply. In contrast, the metabolis-dependent influx of K+(86Rb+) was inhibited in low-K+ plants, while the passive influx in high-K+ plants was stimulated. Translocation of K+ from the roots to the shoots was inhibited by Cd2+ but less influenced in Pb2+-treated plants. It is concluded that the effects of heavy metals depend upon the K+-status of the plants. 相似文献
56.
In this, we consider the coordination of plant growth and ion acquisition, reporting the short-term adjustments of growth and K+ and Na+ relations which follow when plants are subject to a sudden deprivation of N and P. The plant used for the experiments, Spergularia marina (L.) Grieseb., is a small coastal halophyte, and the growth medium was 0.2 × modified seawater. By considering nutrients whose availability has not been changed, we report on an aspect of organismal integration which has received little attention either experimentally or in mathematical models. The studies are limited to the first 60 h after N and P deprivation in order to consider changes that, if they are not primary responses, are not temporally remote, passive adjustments. For growth analyses, plants were used approximately 30 days after germination and 16 days after transfer to solution culture. Random harvests were made at hourly invervals, and after 12 h, one-half of the plants were transferred to cultures without N or P. Tissue analyses were used to calculate relative growth rates, relative accumulation rates and net uptake rates. For comparison, isotope uptake studies using 42K+ and 22Na+ were conducted at 12, 36 and 60 h after deprivation. The effects on growth and biomass allocation were very rapid, detectable within 13 h. K+ transport also responded quickly, and from the beginning of the study, there was essentially no net translocation of K+ to the shoot. Isotope studies confirmed the responsiveness, with translocation reduced 33 and 90% after 12 and 36 h, respectively. Though Na+ adjustments were slower, they were coordinated with growth such that tissue concentrations in the N and P-deprived plants were comparable to those in the controls. We conclude that N and C are insufficient elements on which to build mathematical models useful to environmental physiologists. At a minimum, the incorporation of K+ relations in growth models would both allow the development of the osmotic potential needed to drive cell expansion, and provide a means to probe –experimentally as well as mathematically – the coordinating mechanisms of plant growth and resource management. 相似文献
57.
Carina Ämmälä Krister Bokvist Sheila Galt Patrik Rorsman 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1991,1092(3):347-349
The effects of a photoactivatable (DMNPE-caged) ATP-analogue on ATP-regulated K+-channels (KATP-channel) in mouse pancreatic β-cells were investigated using the inside-out patch configuration of the patch-clamp technique. The caged precursor caused a concentration-dependent reduction of channel activity with a Ki of 17 μM; similar to the 11 μM obtained for standard Mg-ATP. The small difference in the blocking capacity between the precursor and ATP is probably the reason why no change in channel activity was observed upon photolysis of the caged molecule and liberation of ATP. It is suggested that the part of the ATP molecule involved in the blocking reaction of the KATP-channel is not sufficiently protected in DMNPE-caged ATP making this compound unsuitable for studying the rapid kinetics of ATP-induced KATP-channel inhibition. 相似文献
58.
Herbert L. Holland Marc J. Bornmann Gingipalli Lakshmaiah 《Journal of Molecular Catalysis .B, Enzymatic》1996,1(3-6):97-102
Phenyl methyl sulfides substituted in thepara position with methyl, fluoro, chloro, bromo, cyano, nitro, amino, acyl, methoxy, thiomethyl and methylsulfinyl groups have been converted to (S) sulfoxides by biotransformation usingHelminthosporium species NRRL 4671. The highest yields and enantiomeric excesses were obtained with bromo, cyano, methoxy, thiomethyl and methylsulfinyl substituents and in two cases (para-Br and -CN) the products could be crystallized to give (S) sulfoxide of ≥ 96% ee. 相似文献
59.
Increased intracellular calcium concentration ([Ca2+]i) is required for smooth muscle contraction. In tracheal and other tonic smooth muscles, contraction and elevated [Ca2+]i are maintained as long as an agonist is present. To evaluate the physiological role of steady-state increases in Ca2+ on tension maintenance, [Ca2+]i was elevated using ionomycin, a Ca2+ ionophore or charybdotoxin, a large-conductance calcium-activated potassium channel (KCa) blocker prior to or during exposure of tracheal smooth muscle strips to Ach (10–9 to 10–4
M). Ionomycin (5 µM) in resting muscles induced increases in [Ca2+]i to 500±230 nM and small increases in force of 2.6±2.3 N/cm2. This tension is only 10% of the maximal tension induced by ACh. Charybdotoxin had no effect on [Ca2+]i or tension in resting muscle. After pretreatment of muscle with ionomycin, the concentration-response relationship for ACh-induced changes in tension shifted to the left (EC50=0.07±0.05 µM ionomycin; 0.17±0.07 µM, control, p<0.05). When applied to the muscles during steady-state responses to submaximal concentrations of ACh, both ionomycin and charybdotoxin induced further increases in tension. The same magnitude increase in tension occurs after ionomycin and charybdotoxin treatment, even though the increase in [Ca2+]i induced by charybdotoxin is much smaller than that induced by ionomycin. We conclude that the resting muscle is much less sensitive to elevation of [Ca2+]i when compared to muscles stimulated with ACh. Steady-state [Ca2+]i limits tension development induced by submaximal concentrations of ACh. The activity of KCa moderates the response of the muscle to ACh at concentrations less than 1 µM. 相似文献
60.
Kenneth T. Izutsu Sahba Fatherazi Carol M. Belton Dolphine Oda Frank D. Cartwright George E. Kenny 《In vitro cellular & developmental biology. Animal》1996,32(6):361-365
Summary The relations between K+ channel and Cl− channel currents and mycoplasma infection status were studied longitudinally in HSG cells, a human submandibular gland cell
line. The K+ channel currents were disrupted by the occurrence of mycoplasma infection: muscarinic activation of K+ channels and K+ channel expression as estimated by ionomycin- or hypotonically induced K+ current responses were all decreased. Similar decreases in ionomycin- and hypotonically induced responses were observed for
Cl− channels, but only the latter decrease was statistically significant. Also, Cl− currents could be elicited more frequently than K+ currents (63% of cases versus 0%) in infected cells when tested by exposure to hypotonic media, indicating that mycoplasma
infection affects K+ channels relatively more than Cl− channels. These changes occurred in the originally infected cells, were ameliorated when the infection was cleared with sparfloxacin,
and recurred when the cells were reinfected. Such changes would be expected to result in hyposecretion of salivary fluid if
they occurredin vivo. 相似文献