Utilization and management of organic wastes in Chinese agriculture: Past, present and perspectives

Recycling and composting of organic materials such as animal waste, crop residues and green manures has a long tradition in China. In the past, the application of organic manures guaranteed a high return of organic materials and plant mineral nutrients and thus maintained soil fertility and crop yield. As a result of rapid economic development coupled with the increasing urbanization and labour costs, the recycling rate of organic materials in Chinese agriculture has dramatically declined during the last two decades, in particular in the more developed eastern and southeastern provinces of China. Improper handling and storage of the organic wastes is causing severe air and water pollution. Because farmers are using increasing amounts of mineral fertilizer, only 47% of the cropland is still receiving organic manure, which accounted for 18% of N,28% of P and 75% of K in the total nutrient input in 2000. Nowadays, the average proportion of nutrients (N+P+K) supplemented by organic manure in Chinese cropland is only 35% of the total amount of nutrients from both inorganic and organic sources.In China, one of the major causes is the increasing de-coupling of animal and plant production.This is occurring at a time when "re-coupling" is partly being considered in Western countries as a means to improve soil fertility and reduce pollution from animal husbandry. Re-coupling of modern animal and plant production is urgently needed in China. A comprehensive plan to develop intensive animal husbandry while taking into account the environmental impact of liquid and gaseous emissions and the nutrient requirements of the crops as well as the organic carbon requirements of the soil are absolutely necessary. As a consequence of a stronger consideration of ecological aspects in agriculture, a range of environmental standards has been issued and various legal initiatives are being taken in China. Their enforcement should be strictly monitored.     

Ammonium Fixation in Sub-grade Decomposed Granite Substrates

Granitic materials represent a common erosive substrate in California and much of the western United States. When granitic rocks weather, they disintegrate into coarse textured, non-cohesive substrates, known generally as decomposed granite (DG). Because of low moisture and N availability, revegetation of these substrates for erosion control is difficult. If nitrate based fertilizers are used, they can be rapidly leached, while NH ₄ ⁺ fertilizers may be sequestered mineralogically by interlayer fixation. In this study, we focus on the occurrence of NH ₄ ⁺ fixation on a decomposed granitic substrate and show that the fixation capacities of these sandy saprolites are significant despite analyses indicating that the samples are predominantly quartz, have low clay contents and have low cation exchange capacities (CEC). At field loading rates equivalent to less than 300 kg N ha⁻¹, 36–42% of added NH ₄ ⁺ may become unavailable to plants due to interlayer collapse and fixation for an unknown period of time. Ammonium fixation did not vary significantly in relation to substrate weathering class in these samples. Oriented X-ray diffraction analysis revealed the presence of vermiculite in particle size fractions from clay to very coarse sands. While other studies have identified silt as the most active fraction, the relative fixation capacity of these granite saprolites was greatest in the fine and very fine sand fractions when considered on a gram for gram basis of each individual particle size. We found that the extractant cation also influenced the measured levels of NH ₄ ⁺ fixation in these granite saprolites. At loading rates of 0–150 kg NH ₄ ⁺ ha⁻¹, extraction with KCl resulted in apparent NH ₄ ⁺ fixation capacities that were twice as great as those found with NaCl extractions when tested at low NH ₄ ⁺ concentrations and close to 35% greater at higher NH ₄ ⁺ amendment loading. Estimation of available ammonium in the decomposed granite using conventional KCl extraction methods appears to cause fixation, rather than extraction of at least part of the substrate’s NH ₄ ⁺ content.     

Decreased inward rectifier potassium current IK1 in dystrophin-deficient ventricular cardiomyocytes

Kir2.x channels in ventricular cardiomyocytes (most prominently Kir2.1) account for the inward rectifier potassium current I_K1, which controls the resting membrane potential and the final phase of action potential repolarization. Recently it was hypothesized that the dystrophin-associated protein complex (DAPC) is important in the regulation of Kir2.x channels. To test this hypothesis, we investigated potential I_K1 abnormalities in dystrophin-deficient ventricular cardiomyocytes derived from the hearts of Duchenne muscular dystrophy mouse models. We found that I_K1 was substantially diminished in dystrophin-deficient cardiomyocytes when compared to wild type myocytes. This finding represents the first functional evidence for a significant role of the DAPC in the regulation of Kir2.x channels.     

The role of potassium phosphite in chlorophyll fluorescence and photosynthetic parameters of downy mildew-challenged cucumber Cucumis sativus plants

The effect of potassium phosphite (KPhi) on the photosynthetic parameters of cucumber plants inoculated with Pseudoperonospora cubensis was investigated in the present study. Cucumber plants were treated with KPhi before or after inoculation with P. cubensis and leaf samples were collected at different time courses for assessments. Results showed that in pathogen-inoculated plants Fv/Fm was decreased up to 3%. The rate of quantum photosynthetic was also decreased significantly in inoculated plants. Downy mildew led to a decrease in chlorophyll amount which in turn reduced the efficiency of photosystem II. In the KPhi-treated leaves, chlorophyll a and b decreased by 72% and 68%, respectively. Remarkable reduction in the efficiency of photosystem II as well as increased lipid membrane disruption, led to increased lipid peroxidation rate of the membranes up to 52%. The results of this study indicate the mitigating role of potassium phosphite in reducing the adverse effects of pathogen and maintaining the photosynthetic apparatus efficiency in cucumber plants.     

The investigation of interactions of kappa-Hefutoxin1 with the voltage-gated potassium channels: a computational simulation

Kappa-Hefutoxin1 is a K(+) channel-blocking toxin from the scorpion Heterometrus fluvipes. It is a 22-residue protein that adapts a novel fold of two parallel helices linked by two disulfide bridges without beta-sheets. Recognition of interactions of kappa-Hefutoxin1 with the voltage-gated potassium channels, Kv1.1, Kv1.2, and Kv1.3, was studied by 3D-Dock software package. All structures of kappa-Hefutoxin1 were considered during the simulations, which indicated that even small changes in the structure of kappa-Hefutoxin1 considerably affected both the recognition and the binding between kappa-Hefutoxin1 and the Kv1 channels. kappa-Hefutoxin1 is located around the extracellular part of the Kv1 channels, making contacts with its helices. Lys 19, Tyr 5, Arg 6, Trp 9, or Arg 10 in the toxin and residues Asp 402, His 404, Thr 407,Gly 401, and Asp 386 in each subunit of the Kv potassium channel are the key residues for the toxin-channel recognition. Moreover, the simulation result demonstrates that the hydrophobic interactions are important in interaction of negatively charged toxins with potassium channels. The results of our docking/molecular dynamics simulations indicate that our 3D model structure of the kappa-Hefutoxin1-complex is both reasonable and acceptable and could be helpful for smarter drug design and the blocking agents of Kv1 channels.     

Energy distribution and ion selectivity of the bacterial potassium channel

The ion selectivity of the bacterial potassium channel K_CSA is explained upon comparing the energy characteristics of the interaction of cations (Li⁺, Na⁺, K⁺) with atoms of the selectivity filter of the protein pore. Quantum-chemical calculations reveal a deeper potential well for potassium ions, which accounts for preferred K⁺ permeation. It is shown that the conventional methods with AMBER, CHARMM, OPLS force fields in standard parametrization as well as partial re-parametrization give incorrect estimates of ion energy distribution in the channel.     

Heterogeneous expression of TASK-3 and TRAAK in rat paraganglionic cells

In the present study, we investigated the immunohistochemical localization of the two-pore K⁺ channels, TASK-3 and TRAAK, in paraganglionic cells within the superior cervical ganglion, stellate ganglion, and aortic body in comparison with membrane channels in chief cells of the carotid body. TASK-3 immunoreactivity was observed in the paraganglionic cells in all tissues examined. TRAAK immunoreactivity was observed in the chief cells of the aortic body as well as these of the carotid body, but not in the paraganglionic cells in the sympathetic (superior cervical and stellate) ganglia. Our findings indicate that sympathetic paraganglionic cells and glossopharyngeal/vagal paraganglionic cells were different from each other in the expression patterns of TASK-3 and TRAAK to result in the different chemoreception properties of sympathetic paraganglionic cells from those of chief cells of the aortic and carotid bodies.     

Insulinotropic action of glutamate is dependent on the inhibition of ATP-sensitive potassium channel activities in MIN 6 beta cells

To investigate the cellular mechanism of insulinotropic effect of glutamate in pancreatic beta cells, we utilized patch-clamp technique to monitor directly the activities of ATP-sensitive potassium channels (K(ATP) channels). Dimethylglutamate (5mM), a membrane-permeable analog of glutamate, augmented the insulin release induced by the stimulatory concentrations of glucose (p<0.05-0.01). In the cell-attached configurations, dimethylglutamate reversibly and significantly suppressed the K(ATP) channel activities (p<0.01). On the other hand, no significant effect was observed when glutamate itself was applied to the inside-out patches, whereas the prompt and reversible suppression was recorded in the case of ATP (p<0.01). These results indicate that the insulinotropic action of glutamate in beta cells could be derived from the inhibition of K(ATP) channel activities, probably due to generation of messengers via intracellular metabolism such as ATP.     

In vivo topography of Rap1p-DNA complex at Saccharomyces cerevisiae TEF2 UAS(RPG) during transcriptional regulation

We have analyzed in detail the structure of RAP1-UAS(RPG) complexes in Saccharomyces cerevisiae cells using multi-hit KMnO(4), UV and micrococcal nuclease high-resolution footprinting. Three copies of the Rap1 protein are bound to the promoter simultaneously in exponentially growing cells, as shown by KMnO(4) multi-hit footprinting analysis, causing extended and diagnostic changes in the DNA structure of the region containing the UAS(RPG). Amino acid starvation does not cause loss of Rap1p from the complex; however, in vivo UV-footprinting reveals the occurrence of structural modifications of the complex. Moreover, low-resolution micrococcal nuclease digestion shows that the chromatin of the entire region is devoid of positioned nucleosomes but is susceptible to changes in accessibility to the nuclease upon amino acid starvation. The implications of these results for the mechanism of Rap1p action are discussed.     

Crystal structure of the potassium form of an Oxytricha nova G-quadruplex

The crystal structures of the potassium-containing quadruplex formed from the Oxytricha nova sequence d(GGGGTTTTGGGG) are reported, in two space groups, the orthorhombic P2(1)2(1)2(1) and the trigonal P3(2)21, which diffract to 2.0 A and 1.49 A, respectively. The orthorhombic form contains two independent quadruplexes in the asymmetric unit, and the trigonal form contains one. All three of these quadruplexes adopt an identical fold, with two strands forming an antiparallel diagonal arrangement. This is identical with that observed previously in NMR studies of the native sodium and potassium forms, and a crystallographic analysis of it complexed with an O. nova protein. The present analysis demonstrates that the native structure is the same in solution and in the crystalline state and, moreover, that the nature of the counter-ion does not affect the overall fold of this quadruplex. The analysis corrects an earlier crystallographic study of this quadruplex. The conformation of the tetra-thymine loop is described in detail, which involves the third thymine base folding back to interact with the first thymine base. The water networks in the grooves and loops are described and, in particular, the ability of water molecules to form a continuous spine of hydration in the narrow groove is detailed. Each quadruplex has five potassium ions organised in a linear channel, with square antiprismatic coordination to each ion from oxygen atoms.