Use of Heteroduplex Mobility Analysis (HMA) for Differentiating Phytoplasma Isolates Causing Witches' Broom Disease on Populus nigra cv Italica and Stolbur or Big Bud Symptoms on Tomato

Heteroduplex mobility analysis (HMA) was performed to distinguish French and German isolates of phytoplasmas from Populus nigra cv Italica witches broom. The French isolate was similar to the phytoplasma responsible for the European aster yellows while the German isolate was different but closely related to it. When phytoplasmas inducing similar "stolbur" symptoms in tomato were compared to HMA, a high degree of genetic differences was observed among the reference stolbur C (StC) isolate, the European aster yellows and the other phytoplasmas inducing stolbur or big bud symptoms in tomato. Pseudo-stolbur B and D from Brazil were differentiated from the reference St C using this method.     

Impact of waterlogging on the N-metabolism of flood tolerant and non-tolerant tree species

The present study was conducted to characterize the N‐metabolism of important European tree species with different degrees of flooding tolerance. The roots of Fagus sylvatica (sensitive to flooding), Quercus robur (moderately flood tolerant) and Populus tremula × P. alba (flood tolerant) saplings were exposed to different flooding regimes and N uptake, amino acid, protein and chlorophyll concentrations as well as gas exchange were measured. The effects of these treatments on the tree species varied distinctly. In general, the N metabolism of beech was severely affected whereas less impacts were observed on oaks and almost no effects on poplars. The concentrations of amino compounds, particularly of Asp, Asn, Glu and Gln, were lower in the roots of flooded trees than in controls. By contrast, γ‐amino butyric acid concentrations increased. Root protein concentrations remained unaffected in oak and poplar but decreased in beech in response to flooding. The concentrations of pigments remained unaffected by flooding in all tree species investigated. However, photosynthesis and transpiration were severely affected in beech but much less in oak and poplar. The data obtained show a clear correlation between the different flooding tolerances of the trees investigated and the impacts of flooding on N uptake and N metabolism.     

The photosynthetic characteristics of differently shaped leaves in Populus euphratica Olivier

There is a distinct leaf shape polymorphism within a single plant of P. euphratica Olivier. The anatomical structure, carbon isotope discrimination (Δ¹³C), and stomatal and photosynthetic behaviour were investigated in broad-ovate (BOL) and lanceolate (LL) leaves, located at the top and bottom in crown, respectively, of a mature Euphrates poplar growing in its native habitat. Both types of leaves had a non-Kranz anatomy and low Δ¹³C values. However, Δ¹³C of a LL was in average 3.2‰ larger than that of a BOL. In comparison with the LL, the BOL had a smaller stomatal conductance, causing subsequent decreases in transpiration rate and ratio of CO₂ concentrations in intercellular spaces to air. Carbon assimilation rate and water use efficiency were higher in the BOLs than in the LLs. The BOL exhibited C₄-like enzymological features, the activity of glycollate oxidase, and the ratio of activities of ribulose-1,5-bisphosphate carboxylase (RuBPC) to phosphoenolpyruvate carboxylase (PEPC) was lower in BOL than in LL throughout the whole growing season. The lowered ratio of RuBPC/PEPC in BOL was mainly associated with a marked decline in the activity of RuBPC, and only a slight increase in the activity of PEPC. These differences might contribute to microclimate adaptation in both types of leaves. This revised version was published online in June 2006 with corrections to the Cover Date.     

The role of glycine in determining the rate of glutathione synthesis in poplar. Possible implications for glutathione production during stress

The terminal step of glutathione (GSH) synthesis is the condensation of γ-glutamyl-cysteine (γ-EC) with glycine. Relatively little information exists concerning the importance of photorespiratory glycine in determining the rate of conversion of γ-EC to GSH. Consequently, the effect of exogenous glycine and of illumination on foliar contents of γ-EC and GSH was studied in excised leaves and leaf discs from untransformed poplar ( Populus tremula × P. alba ) and poplar overexpressing γ-glutamylcysteine synthetase (γ-ECS; EC 6.3.2.2). Poplars strongly overexpressing γ-ECS (ggs28) had enhanced levels of γ-EC and GSH compared to untransformed poplars. The relationship between γ-EC and GSH contents in ggs28 was light dependent. In illuminated leaves, GSH contents were up to 50-fold higher than γ-EC. On darkening, γ-EC accumulated markedly and GSH declined, so that the GSH:γ-EC ratio was close to 1. These dark-induced changes were prevented by supplying glycine through the petiole or by incubation of leaf discs on glycine. Dark accumulation of γ-EC in leaf discs from untransformed poplar was also prevented by supplying glycine. Supplying cysteine in the dark to discs from untransformed poplar and ggs28 increased γ-EC levels markedly but GSH levels only slightly. Subsequent illumination caused γ-EC to decrease and GSH to increase. Supplying glycine in concert with cysteine had similar effects to illumination. The data suggest that photorespiratory glycine is essential for GSH synthesis, especially under stress conditions, where increased amounts of GSH are required.     

浅层地下水对华北地区河岸杨树林树干液流的影响

华北地区河岸杨树林生长季的用水特征对杨树人工林经营管理具有重要意义.本文以北京市潮白河畔的杨树人工林为研究对象,采用热扩散式探针(TDP)测定树干液流、管式TDR土壤含水量法测定土壤体积含水量、开路涡度相关系统测定环境因子数据,对2014年6—7月的杨树树干液流及其影响因素进行系统研究,以探究浅层地下水对树干液流的可能影响.结果表明: 杨树树干液流日变化随太阳辐射呈现单峰或双峰变化,在土壤水分相对亏缺时,杨树树干液流密度明显减小,达到峰值的时间从14:00提前到12:30,但树干液流对太阳辐射的时滞效应没有明显变化.在表层土壤水分相对充足时,太阳辐射和空气饱和水汽压差是杨树树干液流变化的主导因子,但在表层土壤水分亏缺时,土壤水会成为杨树树干液流的限制因子;当表层土壤水分相对亏缺时,杨树单株耗水与100 cm土层土壤体积含水量呈显著负相关,与其余各土层体积含水量呈显著正相关.浅层地下水(相对稳定的150 cm及更深处)在土壤表层土壤水分含量相对亏缺时,可在毛管力作用下对上层土壤水进行补充,供给杨树生长需要.     

杨和苹果离体茎尖培养和愈伤组织分化与内源IAA、ABA的关系

用杨和苹果的茎尖进行离体培养,在ＭＳ培养基中添加不同浓度的ＢＡ 、ＮＡＡ 或２ ,４Ｄ,诱导（１） 茎的增殖或根的分化,（２） 愈伤组织形成以及愈伤组织分化根、芽或体胚,再生完整植株。测定了不同发育状态器官和组织的内源ＩＡＡ 和ＡＢＡ,并计算了ＩＡＡ／ＡＢＡ 的比值。培养物中其比值较高者,易于诱导不定根分化或形成胚性的愈伤组织,也是木本植物离体培养幼化程度较高的例证。在试管内培养时,杨比苹果培养物易于幼化,因而器官分化、愈伤组织形成和分化、再生完整植株较容易,在ＭＳ 培养基中添加的外源ＢＡ、ＮＡＡ 和２ ,４Ｄ 的浓度也较低。     

涝渍胁迫对杨树苗期叶片生长及其生理性状的影响

以３种典型的美洲黑杨苗木（Ｉ６９、ＮＬ８０１０５和ＮＬ８０３５１）在不同的涝渍胁迫条件下,苗木叶面积生长明显减慢;叶片气孔开度显著减小;叶片水势和丙二醛（ＭＤＡ）含量有所增加;超氧化物歧化酶（ＳＯＤ）活性无明显变化;叶片中的全Ｎ、全Ｐ和全Ｋ含量发生变化。综合分析认为,Ｉ６９杨在强涝渍胁迫下抗耐能力较高。ＮＬ８０３５１杨在弱涝渍胁迫下适应性较强,短期涝渍对杨树无性系苗木无明显影响,３０ｄ以上的涝渍对其影响显著。     

杨,榆混交型林带生长调解试验研究

我国三北地区大面积农田防护林网主要为杨树林网,树种单一,容易造成病虫害蔓延。但由多树种组成的混交林网由于树种生长速度不同,造成树木群体生长参差不齐,而导致某些树木受压抑而不能正常生长甚至死亡,从而降低了林带的防护效益。为改变这一现状并建立起一整套混交...