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51.
Mineral control of organic carbon mineralization in a range of temperate conifer forest soils 总被引:10,自引:1,他引:10
Coupled climate–ecosystem models predict significant alteration of temperate forest biome distribution in response to climate warming. Temperate forest biomes contain approximately 10% of global soil carbon (C) stocks and therefore any change in their distribution may have significant impacts on terrestrial C budgets. Using the Sierra Nevada as a model system for temperate forest soils, we examined the effects of temperature and soil mineralogy on soil C mineralization. We incubated soils from three conifer biomes dominated by ponderosa pine (PP), white fir (WF), and red fir (RF) tree species, on granite (GR), basalt (BS), and andesite (AN) parent materials, at three temperatures (12.5°C, 7.5°C, 5.0°C). AN soils were dominated by noncrystalline materials (allophane, Al‐humus complexes), GR soils by crystalline minerals (kaolinite, vermiculite), and BS soils by a mix of crystalline and noncrystalline materials. Soil C mineralization (ranging from 1.9 to 34.6 [mg C (g soil C)?1] or 0.1 to 2.3 [mg C (g soil)?1]) differed significantly between parent materials in all biomes with a general pattern of ANδ13C values of respired CO2 suggest greater decomposition of recalcitrant soil C compounds with increasing temperature, indicating a shift in primary C source utilization with temperature. Our results demonstrate that soil mineralogy moderates soil C mineralization and that soil C response to temperature includes shifts in decomposition rates, mineralizable pool size, and primary C source utilization. 相似文献
52.
Kinetics of ultrasonic degradation and polymerisation degree distribution of sonochemically degraded chitosans 总被引:1,自引:0,他引:1
The process of physical degradation by means of the ultrasonic action towards chitosans with mole fraction of 2-acetamido-2-deoxy-β-
-glucopyranose units (the degree of N-acetylation, FA) in the range of 0.10≤FA≤0.28, and the weight average polymerisation degree
in the range of
has been investigated. The decrease of
as well as changes in the distribution of the degree of polymerisation (P) has been determined as a function of time, FA, temperature, concentration of chitosan solution and concentration of acetic acid in the solution. The use of low-power ultrasound emitter allowed to establish that in the case of chitosan (binary heteropolysaccharide) the general rate parameter (k) increased with FA. This can be explained by the relatively stronger aggregation of macromolecules with higher FA, which results in size increase of macromolecular individuals and hence in their higher susceptibility to ultrasonic action. It was also observed that k decreased with chitosan concentration and temperature. The value of limiting degree of polimerisation (xe) was found to be influenced by structural parameters of chitosan chains (FA, aggregation). The increase of acetic acid concentration caused the increase in the k value, what indicated accelerating effect of ultrasound towards acidic hydrolysis of chitosan. The shape of the P curve of sonochemically degraded chitosans are in good correlation with the mid-point breakage concept of degradation accepted in sonochemical degradation of polymers. 相似文献
53.
NaCl胁迫对PSII光能利用和耗散的影响 总被引:13,自引:1,他引:13
用荧光动力学的方法研究了不同浓度的NaCl 处理对PSII光能利用和耗散的影响。结果表明,在较低的光强下,与对照、100m mol/L 和200m mol/L NaCl 处理相比,经300m mol/L 和400m mol/L NaCl 处理的小麦,其荧光光化学淬灭效率较低,荧光非光化学淬灭效率较高,Fo 淬灭系数较大,QB - 非还原性PSII反应中心含量较大; 而在较高光强下, 其荧光非光化学淬灭效率和Fo 淬灭系数则相对较低。 相似文献
54.
W. Dörr J. Kummermehr 《Virchows Archiv. B, Cell pathology including molecular pathology》1991,60(1):287-294
Epithelial proliferation in the ventral surface of mouse tongue follows a pronounced circadian rhythm with a peak in mitotic
activity at 10.00 a.m., preceded by a wave of DNA synthesis 8 h earlier. Nearly all cells (85%) pass through G2 and mitosis
immediately after the S-phase; they subsequently divide again, usually after 2 or 3 days, indicating cohorts of cells with
different G1-duration. The fraction of all nucleated cells comprised in one daily proliferation wave is about 20%, indicating
a turnover time of the nucleated cell compartment of about 5 days. Cytotoxic injury by a single radiation dose of 20 Gy causes
a steep decrease in cell counts, leading to complete denudation after 9–13 days. The difference between the latent period
before ulceration and the tissue turnover time is explained by a marked proliferative activity of the doomed cells. The mitotic
index increases steeply after day 1 to three times the control level, but most mitotic figures display gross abnormalities
such as multipolar spindles or chromosome clumping. As a consequence cells with abnormal or multiple nuclei appear in the
basal layers 3 days post irradiation and subsequently migrate to the upper layers. After denudation the epithelium rapidly
becomes restored, with a phase of transient hyperplasia on days 13–14. Normal architecture is regained by day 15. Over the
whole healing period the mitotic index remains at a high level, with most of the mitoses appearing histologically normal. 相似文献
55.
甲基毒死蜱对红细胞膜乙酰胆碱酯酶的抑制作用及其与膜脂相互关系 总被引:1,自引:0,他引:1
以人红细胞膜为材料,研究了甲基毒死蜱与膜上乙酰胆碱酯酶(AChE)的相互作用及其与膜脂的关系。结果显示,甲基毒死蜱对人红细胞膜AChE有明显的抑制作用,与膜温育30min,其半数抑制浓度约为0.10 mmol/L。动力学分析表明,其抑制作用为非竞争性。0.2%Triton X-100并不改变AChE对甲基毒死蜱的敏感性,亦即AChE上甲基毒死蜱的作用部位与其所处的脂质微环境无关。 相似文献
56.
Chuck T. Chen Anthony F. Domenichiello Marc-Olivier Trépanier Zhen Liu Mojgan Masoodi Richard P. Bazinet 《Journal of lipid research》2013,54(9):2410-2422
Brain eicosapentaenoic acid (EPA) levels are 250- to 300-fold lower than docosahexaenoic acid (DHA), at least partly, because EPA is rapidly β-oxidized and lost from brain phospholipids. Therefore, we examined if β-oxidation was necessary for maintaining low EPA levels by inhibiting β-oxidation with methyl palmoxirate (MEP). Furthermore, because other metabolic differences between DHA and EPA may also contribute to their vastly different levels, this study aimed to quantify the incorporation and turnover of DHA and EPA into brain phospholipids. Fifteen-week-old rats were subjected to vehicle or MEP prior to a 5 min intravenous infusion of 14C-palmitate, 14C-DHA, or 14C-EPA. MEP reduced the radioactivity of brain aqueous fractions for 14C-palmitate-, 14C-EPA-, and 14C-DHA-infused rats by 74, 54, and 23%, respectively; while it increased the net rate of incorporation of plasma unesterified palmitate into choline glycerophospholipids and phosphatidylinositol and EPA into ethanolamine glycerophospholipids and phosphatidylserine. MEP also increased the synthesis of n-3 docosapentaenoic acid (n-3 DPA) from EPA. Moreover, the recycling of EPA into brain phospholipids was 154-fold lower than DHA. Therefore, the low levels of EPA in the brain are maintained by multiple redundant pathways including β-oxidation, decreased incorporation from plasma unesterified FA pool, elongation/desaturation to n-3 DPA, and lower recycling within brain phospholipids. 相似文献
57.
Sandeep Kumar Vashist Gregor Czilwik Thomas van Oordt Felix von Stetten Roland Zengerle E. Marion Schneider John H.T. Luong 《Analytical biochemistry》2014
This article reveals a rapid sandwich enzyme-linked immunosorbent assay (ELISA) for the highly sensitive detection of human C-reactive protein (CRP) in less than 30 min. It employs a one-step kinetics-based highly simplified and cost-effective sandwich ELISA procedure with minimal process steps. The procedure involves the formation of a sandwich immune complex on capture anti-human CRP antibody-bound Dynabeads in 15 min, followed by two magnet-assisted washings and one enzymatic reaction. The developed sandwich ELISA detects CRP in the dynamic range of 0.3 to 81 ng ml−1 with a limit of detection of 0.4 ng ml−1 and an analytical sensitivity of 0.7 ng ml−1. It detects CRP spiked in diluted human whole blood and serum with high analytical precision, as confirmed by conventional sandwich ELISA. Moreover, the results of the developed ELISA for the determination of CRP in the ethylenediaminetetraacetic acid plasma samples of patients are in good agreement with those obtained by the conventional ELISA. The developed immunoassay has immense potential for the development of rapid and cost-effective in vitro diagnostic kits. 相似文献
58.
Merve Seven Ü. Cem Derman Andrew J. Harvey 《The Plant journal : for cell and molecular biology》2021,106(6):1660-1673
Xyloglucan endotransglycosylase/hydrolase (XTH) enzymes play important roles in cell wall remodelling. Although previous studies have shown a pathway of evolution for XTH genes from bacterial licheninases, through plant endoglucanases (EG16), the order of development within the phylogenetic clades of true XTHs is yet to be elucidated. In addition, recent studies have revealed interesting and potentially useful patterns of transglycosylation beyond the standard xyloglucan–xyloglucan donor/acceptor substrate activities. To study evolutionary relationships and to search for enzymes with useful broad substrate specificities, genes from the ‘ancestral’ XTH clade of two monocots, Brachypodium distachyon and Triticum aestivum, and two eudicots, Arabidopsis thaliana and Populus tremula, were investigated. Specific activities of the heterologously produced enzymes showed remarkably broad substrate specificities. All the enzymes studied had high activity with the cellulose analogue HEC (hydroxyethyl cellulose) as well as with mixed-link β-glucan as donor substrates, when compared with the standard xyloglucan. Even more surprising was the wide range of acceptor substrates that these enzymes were able to catalyse reactions with, opening a broad range of possible roles for these enzymes, both within plants and in industrial, pharmaceutical and medical fields. Genome screening and expression analyses unexpectedly revealed that genes from this clade were found only in angiosperm genomes and were predominantly or solely expressed in reproductive tissues. We therefore posit that this phylogenetic group is significantly different and should be renamed as the group-IV clade. 相似文献
59.
A. A. Serikov 《Journal of biological physics》1991,18(1):65-77
Model equations for the kinetics of the synthesis and decay of molecular aggregates are used to show the high sensitivity of equilibrium concentrations of high-molecular aggregates to external radiation. This phenomenon is used to explain the effects of low-intensity microwave fields on the functioning of biological systems. The experimental results on the influence of SHF-radiation on ferricyanide reduction by erythrocytes are interpreted in detail. 相似文献
60.
Human heart Na+ channels were expressed transiently in both mammalian cells and Xenopus oocytes, and Na+ currents measured using 150 mM intracellular Na+. The kinetics of decaying outward Na+ current in response to 1-s depolarizations in the F1485Q mutant depends on the predominant cation in the extracellular solution, suggesting an effect on slow inactivation. The decay rate is lower for the alkali metal cations Li+, Na+, K+, Rb+, and Cs+ than for the organic cations Tris, tetramethylammonium, N-methylglucamine, and choline. In whole cell recordings, raising [Na+]o from 10 to 150 mM increases the rate of recovery from slow inactivation at −140 mV, decreases the rate of slow inactivation at relatively depolarized voltages, and shifts steady-state slow inactivation in a depolarized direction. Single channel recordings of F1485Q show a decrease in the number of blank (i.e., null) records when [Na+]o is increased. Significant clustering of blank records when depolarizing at a frequency of 0.5 Hz suggests that periods of inactivity represent the sojourn of a channel in a slow-inactivated state. Examination of the single channel kinetics at +60 mV during 90-ms depolarizations shows that neither open time, closed time, nor first latency is significantly affected by [Na+]o. However raising [Na+]o decreases the duration of the last closed interval terminated by the end of the depolarization, leading to an increased number of openings at the depolarized voltage. Analysis of single channel data indicates that at a depolarized voltage a single rate constant for entry into a slow-inactivated state is reduced in high [Na+]o, suggesting that the binding of an alkali metal cation, perhaps in the ion-conducting pore, inhibits the closing of the slow inactivation gate. 相似文献