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11.
Soares M.M.C.N. Da Silva R. Carmona E.C. Gomes E. 《World journal of microbiology & biotechnology》2001,17(1):79-82
Five Bacillus strains isolated from decaying vegetable material were cultivated on wheat bran and endo-polygalacturonases, exo-polygalacturonase and pectin lyase activities in the crude enzymatic solution obtained were determined. Highest activity was observed for all enzymes when fermentation was carried out at 28 °C, the highest activity values were obtained after 120 h of cultivation for exo-PG and after 48 h for endo-PG and PL. The use of the enzymatic solution for treatment of fruits and vegetable mash afforded a high juice extraction and a pulp with good pressing characteristics. 相似文献
12.
R. V. Mikhailova L. I. Sapunova A. G. Lobanok M. I. Yasenko Zh. F. Shishko 《Microbiology》2000,69(2):162-166
The composition of pectin hydrolase complexes produced by variousAspergillus alliaceus strains was studied under the conditions of induction, catabolite repression, or constitutive synthesis. The strains were
found similar in terms of the polygalacturonase spectrum and different with regard to the levels of endo- and exoenzyme activities.
The analysis of the zymograms of inducible polygalacturonases revealed that all tested cultures contained at least 24 molecular
forms of polygalacturonase. Taking into account only the three molecular forms typical of all analyzed strains ofA. alliaceus with pI values of 5.7, 5.9, and 6.3, one can use the spectrum of constitutive, catabolite repression-resistant polygalacturonases
as an additional taxonomic species criterion. 相似文献