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71.
Micropropagation of a medicinal plant, Plantago major L. 总被引:2,自引:0,他引:2
An efficient micropropagation protocol was developed for an important medicinal plant, Plantago major L. For this purpose,
it is recommended to culture shoot-tips on modified MS medium [412.5 mg dm-3 NH4NO3 and 340 mg dm-3 KH2PO4] supplemented with
50 g dm-3 glucose and 0.5 μM 6-benzylaminopurine. Maximum rooting frequency was obtained at 1 μM naphthaleneacetic acid.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
72.
73.
The effect of auxins and a cytokinin on induction of roots in cultured axillary shoots of Pinus brutia Ten, has been tested. Auxin was crucial for root initiation and the rooting response varied according to the type and concentration of auxin applied. Both auxin and cytokinin and the interactions between them affected the quantity and quality of the induced roots. Aerated non-sterile tap water was an effective rooting medium, comparable to agar. After planting out into soil, some of the influences of auxin and cytokinin could still be seen after six months. However, roots developed normally in the soil, displaying dichotomous lateral branches. The results draw attention to the need for care in the choice and application of the medium for the initial induction of roots. Results of greenhouse trials indicated that the most vigorous plants were obtained via the axillary shoots. 相似文献
74.
Summary The effects of abscisic acid (ABA) (0, 0.09 μM, 0.19 μM, 0.28 μM, and 0.38 μM) or ancymidol (0, 0.98 μM, 1.95 μM, 2.93 μM, 3.90 μM) in embryo germination medium on the conversion of primary embryos to plantlets and secondary embryogenesis were evaluated
for asparagus. ABA and ancymidol each significantly enhanced both responses. ABA was more effective than ancymidol in promoting
the conversion of primary embryos to plantlets, while the converse was true for the production of secondary embryos. The most
effective treatments for embryo conversion were 0.19 and 0.28 μM ABA; 75–77% bipolar and 55–57% globular embryos converted to plantlets. For secondary embryogenesis, the most effective treatments
were 1.95 and 2.93 μM ancymidol; 99–101 and 84–86 somatic embryos were produced from 10 globular and 10 bipolar embryos, respectively. Bipolar
embryos generally converted to plantlets better than globular embryos, but more secondary embryos were produced from globular
embryos than from bipolar embryos in all treatments. ABA and ancymidol also affected the morphology of the plantlets produced.
The plantlets from the embryos incubated on the medium with ancymidol had strong and thick shoots and roots, while those on
the medium with ABA had long, thin shoots and short thin roots. 相似文献
75.
Timir Baran Jha Satyesh Chandra Roy Gopal Chandra Mitra 《Plant Cell, Tissue and Organ Culture》1982,2(1):11-14
Calli from hypocotyl explant of Cuminum cyminum L. (Cumin) grew rapidly on Gamborg's B5 basal medium with the following supplements, (i) 0.5 mg/l — 2,4-D (ii) 4 mg/l — NAA plus 2 mg/l — Kinetin and (iii) 0.2 mg/l — NAA plus 0.2 mg/l — BAP, whereas calli from leaf explant in these media grew slowly. Hypocotyl and leaf calli produced roots when transferred to basal medium only and shoots in basal medium with 0.5 mg/l NAA and 0.1 mg/l BAP. Ninety percent of the shoots produced roots when they were transferred to half strength MS inorganic salts supplemented with 0.5 mg/l each of IBA and NAA.Fifty to sixty percent of rootless as well as rooted shoots produced terminal umbellate flowers on this medium. 相似文献
76.
根据植物顶端生长优势的原理,在猕猴桃(Actinidia chinensis)胚乳培养中,将愈伤组织上分化的芽,切割下来作接穗,利用简单装置,进行“嫩枝顶端嫁接”,嫁接成活率高达90%左右,并至少提前1—2年开花、结果。利用嫩枝顶端嫁接技术,不仅提高了试管苗的成活率,而且在简化培养程序、缩短培养周期、降低培养成本上都有明显的效果。 相似文献
77.
Callus cultures were established from bulb explants of diploid Urginea indica Kunth (Indian squill) on a modified basal medium of Murashige and Skoog (1962) supplemented with either 2 mg/l-1 2,4-D+15% (v/v) CM or 4 mg/l-1 2,4-D+2 mg/l-1 NAA+2 mg/l-1 KN+1 g/l-1 YE. Shoot primordia developed after 2–3 subcultures in that medium. Increased growth of shoot primordia was obtained in media containing less auxins and vitamins. Rooted bulbous plantlets obtained were maintained in MS medium with 0.5% sucrose.Adventitious shoots were induced from adaxial epidermal cells of outer scales of regenerated bulbs used as secondary expiants in presence of 1 mg/l-1 of 2,4-D with slightly higher concentration of the three vitamins of MS medium. From each scale leaf, approximately 400 bulblets were produced in 18 weeks in liquid culture. 90% of the plants transferred to potted soil have survived. 相似文献
78.
Light quality influences germination, root growth and hypocotyl elongation in somatic embryos but not in seedlings of norway spruce 总被引:4,自引:0,他引:4
Harald Kvaalen Maigull Appelgren 《In vitro cellular & developmental biology. Plant》1999,35(6):437-441
Summary We studied how light from different light sources influences germination and postgerminative growth of plants from somatic
embryos and seeds of Norway spruce (Picea abies [L.] Karst). Somatic embryos of three spruce genotypes and seeds were subjected to light from commercially available light
sources: Philips TLD Blue 18W/18 (BL), Osram Fluora (FL), Philips Cool White TL 50W/33 (CW), Osram Warm White 18W/30 (WW),
Philips Yellow 36W/16 (YE) and Philips TLD Red 36W/15 (RE), 18 h a day, with a photon flux (PAR) at 30 μmol m−2 s−1. After 6 wk the germination frequencies of the somatic embryo-derived plantlets were 50% under BL and 98% under RE. The corresponding
mean root lengths were 6.7 and 15.4 mm. In somatic embryo-derived plantlets cultured under BL, FL, CW and WW, both roots and
hypocotyls turned brown, presumably due to production of phenolic substances. Browning was less severe in somatic embryo-derived
plantlets cultured under RE and YE. Under RE, the epicotyl elongated in 37% of the plantlets after 6 wk, compared with 70%
under the other light sources. Seed germination and postgerminative seedling growth was modestly influenced by light from
these light sources. RE and WW initially delayed germination as compared with BL, FL and CW, but after 2 wk, more than 90%
of the seeds had germinated under all light sources. In conclusion, germination and postgerminative growth of somatic embryos
of spruce is sensitive to differences in light quality, whereas seed germination and seedling growth is not. 相似文献
79.
High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng 总被引:4,自引:0,他引:4
W. Tang 《Plant cell reports》2000,19(7):727-732
The morphogenesis ability of light yellowish globular callus derived from cotyledons of mature zygotic embryos of Panax ginseng was investigated. The optimal media for somatic embryogenesis and shoot organogenesis were MS medium containing 0.5 mg l–1 2,4-dichlorophenoxyacetic acid, 0.1 mg l–1 6-benzyladenine (BA), and 500 mg l–1 lactoalbumin hydrolysate, and SH medium supplemented with 0.5 mg l–1 α-naphthaleneacetic acid, 0.1 mg l–1 BA, and 500 mg l–1casein hydrolysate. The influences of glucose, mannose, fructose, and sorbose in the media on somatic embryogenesis and shoot
organogenesis were revealed as differences in the numbers of somatic embryos and adventitious shoots per gram of morphogenic
callus. The best regeneration of somatic embryos was obtained on medium containing glucose, with a mean of 8.7 somatic embryos
per gram of callus. The best regeneration of shoots was observed on medium containing fructose, with an average of 12.2 adventitious
shoots per gram of callus. Of the somatic embryos 95% were converted into regenerated plantlets, and 100% of adventitious
shoots rooted to form regenerated plantlets. Regenerated plants were successfully established in soil. Flowering was observed
in 5.7% of the regenerated plants derived from shoot organogenesis and in 1.4% of the regenerated plants derived from somatic
embryogenesis.
Received: 1 December 1998 / Revision received: 13 September 1999 / Accepted: 20 September 1999 相似文献
80.
罗汉果组培苗生物学特性研究 总被引:1,自引:1,他引:0
对罗汉果组培苗生育周期、生长发育习性以及生态适应性进行观测,结果表明:年全生育期为240~260d,定植当年即可正常开花结实。根系与块茎有两个增长高峰期,分别为开花结果期(7月上、中旬)、枯苗期(11月上旬~12月上旬);茎蔓在定植20d内生长较缓慢,之后逐步加快,其中主蔓和一级侧蔓构成植株空间骨架结构,二级侧蔓和三级侧蔓为主要结果蔓;开花座果盛期在7月下旬至8月中旬,花、果着生位置以二级侧蔓的6~15节和三级侧蔓的3~18节为主,点花授粉宜在雌雄花开放的当天上午进行,果实的生长膨大约在座果后的30d内完成,但果实发育成熟约需80d。生长发育的适温为22~28℃,其中旬温25~28℃时对花果生育最为适宜;生长过程中应保持土壤湿润和80%以上的空气湿度;全生育期对氮和钾的吸收量较大,但在生殖生长期,尤其在结果盛期与果实发育期,对磷、钾的需求增加。 相似文献