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41.
Structural determinants of increased susceptibility to dehydration‐induced cavitation in post‐fire resprouting chaparral shrubs 下载免费PDF全文
Anna L. Jacobsen Michael F. Tobin Hayden S. Toschi Marta I. Percolla R. Brandon Pratt 《Plant, cell & environment》2016,39(11):2473-2485
It is well established that transpiration and photosynthetic rates generally increase in resprouting shoots after fire in chaparral shrublands. By contrast, little is known about how plant hydraulic function varies during this same recovery period. We hypothesized that vascular traits, both functional and structural, would also shift in order to support this heightened level of gas exchange and growth. We examined stem xylem‐specific hydraulic conductivity (Ks) and resistance to cavitation (P50) for eight chaparral shrub species as well as several potential xylem structural determinants of hydraulic function and compared established unburned plants and co‐occurring post‐fire resprouting plants. Unburned plants were generally more resistant to cavitation than resprouting plants, but the two groups did not differ in Ks. Resprouting plants had altered vessel structure compared with unburned plants, with resprouting plants having both wider diameter vessels and higher inter‐vessel pit density. For biomechanics, unburned plants had both stronger and denser stem xylem tissue than resprouting plants. Shifts in hydraulic structure and function resulted in resprouting plants being more vulnerable to dehydration. The interaction between time since disturbance (i.e. resprouting versus established stands) and drought may complicate attempts to predict mortality risk of resprouting plants. 相似文献
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The pit organs of elasmobranchs (sharks, skates and rays) are free neuromasts of the mechanosensory lateral line system. Pit organs, however, appear to have some structural differences from the free neuromasts of bony fishes and amphibians. In this study, the morphology of pit organs was investigated by scanning electron microscopy in six shark and three ray species. In each species, pit organs contained typical lateral line hair cells with apical stereovilli of different lengths arranged in an “organ‐pipe” configuration. Supporting cells also bore numerous apical microvilli taller than those observed in other vertebrate lateral line organs. Pit organs were either covered by overlapping denticles, located in open grooves bordered by denticles, or in grooves without associated denticles. The possible functional implications of these morphological features, including modification of water flow and sensory filtering properties, are discussed. J. Morphol. 2009. © 2009 Wiley‐Liss, Inc. 相似文献
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Duan Devetak 《Physiological Entomology》2005,30(1):82-86
Abstract. Pit-building antlions are predators with a unique predation strategy, namely using pitfall traps constructed in loose sand to catch prey. Here, prey escape-time in the field is measured by introducing ants into one of four different treatment arenas. The first treatment lacks pits and antlions, the second includes 10 antlions that did not build pits, the third comprises eight artificially constructed pits, and the fourth is a treatment of eight antlions in pits and two without pits. Their pits are of a similar size to those used in the third treatment. When antlions are present without constructing pits, they impede the dispersal of prey. The mean escape-time for one half of the released ants is twice as long with antlions present as without them. When pits are present, the time taken for one half of the released ants to escape the predator is more than 10 times as long as when pits are absent. Escape-time from artificial pits is three times that from nonpit building antlions. Pits hinder the escape of ants and therefore increase the amount of time that the prey is available for capture. In the area where the pits are occupied by antlions, escape-time is four times longer than in a treatment with similar sized artificial pits. Thus, it appears that not only a pit, but also the presence of antlions influences the capture success. 相似文献
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Parasitic red algae grow only on other red algae and have over 120 described species. Developmental studies in red algal parasites are few, although they have shown that secondary pit connections formed between parasite and host and proposed that this was an important process in successful parasitism. Furthermore, it was recorded that the transfer of parasite nuclei by these secondary pit connections led to different host cell effects. We used developmental studies to reconstruct early stages and any host cell effects of a parasite on Vertebrata aterrima. A mitochondrial marker (cox1) and morphological observations (light and fluorescence microscopy) were used to describe this new red algal parasite as Vertebrata aterrimophila sp. nov. Early developmental stages show that a parasite spore connects via secondary pit connections with a pericentral host cell after cuticle penetration. Developmental observations revealed a unique connection cell that grows into a ‘trunk-like’ structure. Host cell transformation after infection by the parasite included apparent increases in both carbohydrate concentrations and nuclear size, as well as structural changes. Analyses of molecular phylogenies and reproductive structures indicated that the closest relative of V. aterrimophila is its host, V. aterrima. Our study shows a novel developmental parasite stage (‘trunk-like’ cell) and highlights the need for further developmental studies to investigate the range of developmental patterns and host effects in parasitic red algae. 相似文献
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用兔抗人催乳素多克隆抗体和鼠抗人催乳索受体单克隆抗体对文昌鱼神经系统、哈氏窝和其它组织进行免疫组织化学研究。结果显示:催乳素免疫活性细胞及催乳素受体定位在文昌鱼脑泡、神经管、哈氏窝、轮器、内柱、消化管和性腺(卵巢和精巢),表明催乳素在文昌鱼有广泛分布,并且从进化观点来看,证明催乳素是一种高度保守的古老激素。双重免疫染色进一步揭示催乳素及其受体免疫反应阳性物质共存于同一卵母细胞胞膜和胞质以及精巢中精原细胞、初级与次级精母细胞和Sertoli细胞。研究结果首次证明了文昌鱼脑泡和哈氏窝以及其它组织能够合成和分泌催乳素,表明像脊椎动物一样,催乳素可能参与调节文昌鱼体内代谢和对环境的适应以及性腺发育,提示文昌鱼可能出现原始的脑泡-哈氏窝(催乳素)-靶细胞调控轴的雏形。本研究为文昌鱼哈氏窝内分泌学以及催乳素的起源与演化提供新的基础资料。 相似文献
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As an insect pathogen, Photorhabdus luminescens possesses an arsenal of toxins. Here we cloned and expressed a probable toxin from P. luminescens subsp. akhurstii YNd185, designated as Photorhabdus insecticidal toxin (Pit). The pit gene shares 94% nucleotide and 98% predicted amino acid sequence identity with plu1537, a predicted ORF from P. luminescens subsp. laumondii TT01 and 30% predicted amino acid sequence similarity to a fragment of a 13.6 kDa insecticidal crystal protein gene of Bacillus thuringiensis (Bt). The pit was expressed as a GST-Pit fusion protein in E. coli, most of which was insoluble and sequestered into inclusion bodies. The inclusion bodies were harvested and dissolved. The
resultant protein was purified and the Pit was cleaved from the fusion protein by thrombin and purified from GST then used
for bioassay. Pit killed Galleria mellonella (LD50, 30 ng/larva) and Spodoptera litura (LD50, 191 ng/larva) via hemocoel injection. Relative to a control that lacked toxin, Pit did not significantly increase mortality
of S. litura and Helicoverpa armigera when introduced orally, but the treatment did inhibit growth of the insects. The present study demonstrated that Pit possessed
insecticidal activity. 相似文献
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