全文获取类型
收费全文 | 3537篇 |
免费 | 256篇 |
国内免费 | 447篇 |
出版年
2024年 | 7篇 |
2023年 | 44篇 |
2022年 | 42篇 |
2021年 | 75篇 |
2020年 | 96篇 |
2019年 | 96篇 |
2018年 | 92篇 |
2017年 | 91篇 |
2016年 | 113篇 |
2015年 | 102篇 |
2014年 | 160篇 |
2013年 | 219篇 |
2012年 | 188篇 |
2011年 | 227篇 |
2010年 | 188篇 |
2009年 | 239篇 |
2008年 | 223篇 |
2007年 | 220篇 |
2006年 | 212篇 |
2005年 | 179篇 |
2004年 | 185篇 |
2003年 | 159篇 |
2002年 | 148篇 |
2001年 | 101篇 |
2000年 | 83篇 |
1999年 | 91篇 |
1998年 | 58篇 |
1997年 | 65篇 |
1996年 | 48篇 |
1995年 | 56篇 |
1994年 | 57篇 |
1993年 | 26篇 |
1992年 | 40篇 |
1991年 | 26篇 |
1990年 | 37篇 |
1989年 | 27篇 |
1988年 | 20篇 |
1987年 | 17篇 |
1986年 | 23篇 |
1985年 | 46篇 |
1984年 | 34篇 |
1983年 | 19篇 |
1982年 | 17篇 |
1981年 | 8篇 |
1980年 | 4篇 |
1979年 | 17篇 |
1978年 | 6篇 |
1976年 | 4篇 |
1973年 | 2篇 |
1972年 | 2篇 |
排序方式: 共有4240条查询结果,搜索用时 695 毫秒
101.
102.
Luo Shengxue Zhang Panli Zou Peng Wang Cong Liu Bochao Wu Cuiling Li Tingting Zhang Ling Zhang Yuming Li Chengyao 《中国病毒学》2021,36(5):1113-1123
Virologica Sinica - SARS-CoV-2 has caused more than 3.8 million deaths worldwide, and several types of COVID-19 vaccines are urgently approved for use, including adenovirus vectored vaccines.... 相似文献
103.
J. Fraser Wright 《Biotechnology journal》2021,16(1):2000022
For adeno-associated virus (AAV)-based human gene therapy, challenges for the translation of promising research results to successful clinical development include optimization of vector design and manufacturing processes to ensure that vectors prepared for administration to human subjects have attributes consistent with safe and durable expression. This article briefly reviews quality control methods for routine testing and supplemental characterization of AAV vectors for investigational product development. The relationship of vector and manufacturing process design with product critical quality attributes is discussed. 相似文献
104.
In order to make renewable fuels and chemicals from microbes, new methods are required to engineer microbes more intelligently. Computational approaches, to engineer strains for enhanced chemical production typically rely on detailed mechanistic models (e.g., kinetic/stoichiometric models of metabolism)—requiring many experimental datasets for their parameterization—while experimental methods may require screening large mutant libraries to explore the design space for the few mutants with desired behaviors. To address these limitations, we developed an active and machine learning approach (ActiveOpt) to intelligently guide experiments to arrive at an optimal phenotype with minimal measured datasets. ActiveOpt was applied to two separate case studies to evaluate its potential to increase valine yields and neurosporene productivity in Escherichia coli. In both the cases, ActiveOpt identified the best performing strain in fewer experiments than the case studies used. This work demonstrates that machine and active learning approaches have the potential to greatly facilitate metabolic engineering efforts to rapidly achieve its objectives. 相似文献
105.
为构建西方马脑炎病毒(western equine encephalomyelitis virus,WEEV)结构基因C-E3-E2-6k-E1重组真核表达载体,并研究其作为核酸疫苗的免疫原性.采用PCR方法扩增目的基因,酶切之后连接到pcDNA3.1上构建真核表达载体pcDNA3.1-C-E,用酶切和测序分析方法鉴定正确后,重组质粒被转染到293T细胞,经电镜检测和间接免疫荧光方法证明基因可以表达后,用该重组质粒免疫小鼠,免疫后检测实验组小鼠外周血中CD4+T细胞/CD8+T细胞比例和血清中细胞因子IL-2、IL-4及IFN-γ浓度,以上实验组各项免疫指标与对照组相比差异均显著(P< 0.05);ELISA方法检测实验组小鼠血清中WEEV的IgG抗体效价是1∶16.研究结果表明重组质粒pcDNA3.1-C-E可在细胞中获得瞬时表达,并且重组质粒作为核酸疫苗能够刺激小鼠产生免疫反应,具有较强免疫原性,为今后WEEV核酸疫苗研制奠定了良好基础. 相似文献
106.
目的:构建4E-BP1及其 T37A、T46A、S65A、T70A 突变体4E-BP1-4A 基表达的重组慢病毒载体,研究其对胃癌 HGC27细胞生长的影响.方法:PCR 扩增4E-BP1基及其突变体4E-BP1-4A 基并克隆到 pCDH 载体,构建成 pCDH-4E-BP1、pCDH-4E-BP1-4A,将其与包装载体共转染293T 细胞,包装成 Lenti-4E-BP1及 Lenti-4E-BP1-4A重组慢病毒载体,将此慢病毒感染胃癌 HGC27细胞,Western 印迹鉴定病毒载体介导的4E-BP1、4E-BP1-4A 蛋白的表达,MTT、克隆形成和软琼脂方法研究过量表达4E-BP1、4E-BP1-4A 对胃癌 HGC27细胞生长的影响.结果:包装成 Lenti-4E-BP1及 Lenti-4E-BP1-4A 重组慢病毒载体,并将此慢病毒载体感染胃癌 HGC27细胞;MTT、克隆形成、软琼脂实验表明过量表达4E-BP1可抑制胃癌 HGC27细胞的生长,过量表达4E-BP1-4A 时抑制效果更明显.结论:构建了4E-BP1、4E-BP1-4A 的重组慢病毒表达载体,在胃癌 HGC27细胞中过量表达4E-BP1可抑制细胞生长,过量表达4E-BP1-4A 的抑制效果更明显. 相似文献
107.
108.
《Expert review of proteomics》2013,10(1):113-126
Human and animal African trypanosomoses, or sleeping sickness and Nagana, are neglected vector-borne parasitic diseases caused by protozoa belonging to the Trypanosoma genus. Advances in proteomics offer new tools to better understand host–vector–parasite crosstalks occurring during the complex parasitic developmental cycle, and to determine the outcome of both transmission and infection. In this review, we summarize proteomics studies performed on African trypanosomes and on the interactions with their vector and mammalian hosts. We discuss the contributions and pitfalls of using diverse proteomics tools, and argue about the interest of pathogenoproteomics, both to generate advances in basic research on the best knowledge and understanding of host–vector–pathogen interactions, and to lead to the concrete development of new tools to improve diagnosis and treatment management of trypanosomoses in the near future. 相似文献
109.
Masaaki Hayafune Naoko Miyano-Kurosaki Hiroshi Takaku Wee-Sung Park 《Nucleosides, nucleotides & nucleic acids》2013,32(7):795-799
The RNA interference (RNAi) phenomenon is a recently observed process in which the introduction of a double-stranded RNA (dsRNA) into cells causes the specific degradation of an mRNA containing the same sequence. To study dsRNA-mediated gene interference targeted to the env gene (NL4-3: 7490-7508) in HIV-1 infected cells, we constructed tandem-type and hairpin-type siRNA expression vectors, which were under the control of two U6 promoters. We also constructed lentiviral-based siRNA expression vectors for further assessment of their antiviral activity in transduced cells. At both the transient plasmid and lentiviral-mediated RNA expression levels, the siRNA encoding the env fragment exhibited sequence-specific suppression of target gene expression and strongly inhibited (≥90%) HIV-1 infection in the cells, as compared to the antisense RNA expression vector. Targeting the HIV-1 env gene with siRNAs encoding the env gene fragment (7490–7508) might be an effective strategy for gene therapy applications in HIV-1/AIDS treatment and management. 相似文献
110.