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221.
222.
Preference and avoidance pH of brook trout Salvelinus fontinalis and brown trout Salmo trutta exposed to different holding pH
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The goal of this study was to determine if short‐term exposure of brook trout Salvelinus fontinalis and brown trout Salmo trutta to a lower pH than found in their source stream results in a shift in preference or avoidance pH. The lack of a shift in preference or avoidance pH of adult S. fontinalis and S. trutta suggests that these species can be held at a pH different from the source waterbody for a short period of time without altering preference or avoidance pH behaviour. 相似文献
223.
Integrating metabolic performance,thermal tolerance,and plasticity enables for more accurate predictions on species vulnerability to acute and chronic effects of global warming
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Predicting species vulnerability to global warming requires a comprehensive, mechanistic understanding of sublethal and lethal thermal tolerances. To date, however, most studies investigating species physiological responses to increasing temperature have focused on the underlying physiological traits of either acute or chronic tolerance in isolation. Here we propose an integrative, synthetic approach including the investigation of multiple physiological traits (metabolic performance and thermal tolerance), and their plasticity, to provide more accurate and balanced predictions on species and assemblage vulnerability to both acute and chronic effects of global warming. We applied this approach to more accurately elucidate relative species vulnerability to warming within an assemblage of six caridean prawns occurring in the same geographic, hence macroclimatic, region, but living in different thermal habitats. Prawns were exposed to four incubation temperatures (10, 15, 20 and 25 °C) for 7 days, their metabolic rates and upper thermal limits were measured, and plasticity was calculated according to the concept of Reaction Norms, as well as Q10 for metabolism. Compared to species occupying narrower/more stable thermal niches, species inhabiting broader/more variable thermal environments (including the invasive Palaemon macrodactylus) are likely to be less vulnerable to extreme acute thermal events as a result of their higher upper thermal limits. Nevertheless, they may be at greater risk from chronic exposure to warming due to the greater metabolic costs they incur. Indeed, a trade‐off between acute and chronic tolerance was apparent in the assemblage investigated. However, the invasive species P. macrodactylus represents an exception to this pattern, showing elevated thermal limits and plasticity of these limits, as well as a high metabolic control. In general, integrating multiple proxies for species physiological acute and chronic responses to increasing temperature helps providing more accurate predictions on species vulnerability to warming. 相似文献
224.
María A. Bausero 《Cell stress & chaperones》2015,20(5):759-766
The First Conference of the Latin America Chapter of the Cell Stress Society International (CSSI) organized by CSSI was held in Montevideo, Uruguay, on March 11–14, 2014. The Latin America Chapter of the CSSI (LAC-CSSI) was established at the Workshop on the Molecular Biology of the Stress Response, Porto Alegre, Brazil, May 2012. The chapter’s first meeting took place in the beautiful city of Montevideo and was chaired by the first (LAC-CSSI) elected president Professor María Bausero. Forty-two invited speakers presented their work to more than 100 scientists. The first day of the conference was dedicated to an introductory program for students, young investigators, and participants new to the field of molecular chaperones and the stress response. These seminars were held in the Pasteur Institute of Montevideo and the Faculty of Sciences of the University of the Republic. These institutions were carefully selected to give foreign participants a broad view of the diversity of students and institutions doing research in Uruguay, as well as an opportunity for direct interaction with our scientists and students. Invited speakers for the seminar series were Dr. Wolfgang Schumann, Dr. Cristina Bonorino, Dr. Antonio De Maio, Dr. Ian Brown, Dr. Rafael Radi, Dr. Daniel Ciocca, and Dr. Celia Quijano. The remaining days of the conference took place at the Sheraton Hotel in Montevideo, and the scientific sessions are discussed below. 相似文献
225.
The effect of lichen‐dominated biological soil crusts on growth and physiological characteristics of three plant species in a temperate desert of northwest China
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Biocrusts (biological soil crusts) cover open spaces between vascular plants in most arid and semi‐arid areas. Information on effects of biocrusts on seedling growth is controversial, and there is little information on their effects on plant growth and physiology. We examined impacts of biocrusts on growth and physiological characteristics of three habitat‐typical plants, Erodium oxyrhynchum, Alyssum linifolium and Hyalea pulchella, growing in the Gurbantunggut Desert, northwest China. The influence of biocrusts on plant biomass, leaf area, leaf relative water content, photosynthesis, maximum quantum efficiency of PSII (Fv/Fm), chlorophyll, osmotic solutes (soluble sugars, protein, proline) and antioxidant enzymes (superoxide dismutase, catalase, peroxidase) was investigated on sites with or without biocrust cover. Biomass, leaf area, leaf water content, photosynthesis, Fv/Fm and chlorophyll content in crusted soils were higher than in uncrusted soils during early growth and lower later in the growth period. Soluble sugars, proline and antioxidant enzyme activity were always higher in crusted than in uncrusted soils, while soluble protein content was always lower. These findings indicate that biocrusts have different effects on these three ephemeral species during growth in this desert, primarily via effects on soil moisture, and possibly on soil nutrients. The influence of biocrusts changes during plant development: in early plant growth, biocrusts had either positive or no effect on growth and physiological parameters. However, biocrusts tended to negatively influence plants during later growth. Our results provide insights to explain why previous studies have found different effects of biocrusts on vascular plant growth. 相似文献
226.
227.
Jasmonic acid perception by COI1 involves inositol polyphosphates in Arabidopsis thaliana 总被引:1,自引:0,他引:1
Mosblech A Thurow C Gatz C Feussner I Heilmann I 《The Plant journal : for cell and molecular biology》2011,65(6):949-957
Plant responses to wounding are part of their defense responses against insects, and are tightly regulated. The isoleucin conjugate of jasmonic acid (JA‐Ile) is a major regulatory molecule. We have previously shown that inositol polyphosphate signals are required for defense responses in Arabidopsis; however, the way in which inositol polyphosphates contribute to plant responses to wounding has so far remained unclear. Arabidopsis F‐box proteins involved in the perception of JA‐Ile (COI1) and auxin (TIR1) are structurally similar. Because TIR1 has recently been shown to contain inositol hexakisphosphate (InsP6) as a co‐factor of unknown function, here we explored the possibility that InsP6 or another inositol polyphosphate is required for COI1 function. In support of this hypothesis, COI1 variants with changes in putative inositol polyphosphate coordinating residues exhibited a reduced interaction with the COI1 target, JAZ9, in yeast two‐hybrid tests. The equivalent COI1 variants displayed a reduced capability to rescue jasmonate‐mediated root growth inhibition or silique development in Arabidopsis coi1 mutants. Yeast two‐hybrid tests using wild‐type COI1 in an ipk1Δ yeast strain exhibiting increased levels of inositol pentakisphosphate (InsP5) and reduced levels of InsP6 indicate an enhanced COI1/JAZ9 interaction. Consistent with these findings, Arabidopsis ipk1‐1 mutants, also with increased InsP5 and reduced InsP6 levels, showed increased defensive capabilities via COI1‐mediated processes, including wound‐induced gene expression, defense against caterpillars or root growth inhibition by jasmonate. The combined data from experiments using mutated COI1 variants, as well as yeast and Arabidopsis backgrounds altered in inositol polyphosphate metabolism, indicate that an inositol polyphosphate, and probably InsP5, contributes to COI1 function. 相似文献
228.
Chen MK Hsu WH Lee PF Thiruvengadam M Chen HI Yang CH 《The Plant journal : for cell and molecular biology》2011,68(1):168-185
The ectopic expression of a MADS box gene FOREVER YOUNG FLOWER (FYF) caused a significant delay of senescence and a deficiency of abscission in flowers of transgenic Arabidopsis. The defect in floral abscission was found to be due to a deficiency in the timing of cell separation of the abscission zone cells. Down-regulation of INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) may contribute to the delay of the floral abscission in 35S:FYF flowers. FYF was found to be highly expressed in young flowers prior to pollination and was significantly decreased after pollination, a pattern that correlated with its function. Ethylene insensitivity in senescence/abscission and the down-regulation of ETHYLENE RESPONSE DNA-BINDING FACTOR 1 (EDF1) and EDF2, downstream genes in the ethylene response, in 35S:FYF Arabidopsis suggested a role for FYF in regulating senescence/abscission by suppressing the ethylene response. This role was further supported by the fact that 35S:FYF enhanced the delay of flower senescence/abscission in ethylene response 1 (etr1), ethylene-insensitive 2 (ein2) and constitutive triple response 1 (ctr1) mutants, which have defects in upstream genes of the ethylene signaling pathway. The presence of a repressor domain in the C-terminus of FYF and the enhancement of the delay of senescence/abscission in FYF+SRDX (containing a suppression motif) transgenic plants suggested that FYF acts as a repressor. Indeed, in FYF-DR+VP16 transgenic dominant-negative mutant plants, in which FYF was converted to a potent activator by fusion to a VP16-AD motif, the senescence/abscission of the flower organs was significantly promoted, and the expression of BOP2, IDA and EDF1/2 was up-regulated. Our data suggest a role for FYF in controlling floral senescence/abscission by repressing ethylene responses and regulating the expression of BOP2 and IDA in Arabidopsis. 相似文献
229.
iTRAQ-based analysis of changes in the cassava root proteome reveals pathways associated with post-harvest physiological deterioration 总被引:1,自引:0,他引:1
Owiti J Grossmann J Gehrig P Dessimoz C Laloi C Hansen MB Gruissem W Vanderschuren H 《The Plant journal : for cell and molecular biology》2011,67(1):145-156
The short storage life of harvested cassava roots is an important constraint that limits the full potential of cassava as a commercial food crop in developing countries. We investigated the molecular changes during physiological deterioration of cassava root after harvesting using isobaric tags for relative and absolute quantification (iTRAQ) of proteins in soluble and non‐soluble fractions prepared during a 96 h post‐harvest time course. Combining bioinformatic approaches to reduce information redundancy for unsequenced or partially sequenced plant species, we established a comprehensive proteome map of the cassava root and identified quantitatively regulated proteins. Up‐regulation of several key proteins confirmed that physiological deterioration of cassava root after harvesting is an active process, with 67 and 170 proteins, respectively, being up‐regulated early and later after harvesting. This included regulated proteins that had not previously been associated with physiological deterioration after harvesting, such as linamarase, glutamic acid‐rich protein, hydroxycinnamoyl transferase, glycine‐rich RNA binding protein, β‐1,3‐glucanase, pectin methylesterase, maturase K, dehydroascorbate reductase, allene oxide cyclase, and proteins involved in signal pathways. To confirm the regulation of these proteins, activity assays were performed for selected enzymes. Together, our results show that physiological deterioration after harvesting is a highly regulated complex process involving proteins that are potential candidates for biotechnology approaches to reduce such deterioration. 相似文献
230.
Kela P. WeberRaymond L. Legge 《Ecological Engineering》2011,37(5):666-677
The objective of this work was to study the effect of plant presence (Phragmites australis) and inoculant origin on wetland mesocosm start-up dynamics. Eight mesocosms were studied based on a duplicated 22 factorial design tracking bacterial community and hydrological changes during an 8 month start-up period. The mesocosms were characterized in terms of their hydrological character based on evapotranspiration (ET), porosity, and a dispersion coefficient. The microbiological regime was characterized using a microbial activity measure and community-level physiological profiling (CLPP) employing BIOLOG™ ECO plates. CLPP-related indices such as substrate richness, substrate diversity, over-all community profile, and community divergence are also presented. It was found that mesocosm porosities decreased over time as a result of media-related biofilm development. This biofilm development also contributed to a substantial increase in the dispersion coefficient in the mesocosms over the start-up period. Dispersion coefficients in planted systems reached values of ∼50-55 cm2/min whereas in the unplanted systems values of ∼30-35 cm2/min were observed. Bacterial community divergence in the mesocosms was quantified using a Euclidean-based divergence metric. All mesocosms showed a sharp increase in community divergence until day 75, at which point a steady state was reached. The interstitial communities were also characterized in terms of similarity based on the experimental design treatments. Four stages of mesocosm development were identified that can be described by an initial community state based on the origins of the initial inoculum [days 0-6]; a dynamic period where adjustments and shifts in the bacterial community occurred in all mesocosms [days 7-26]; a period where all interstitial CLPPs were quite similar [days 27-73]; and finally a shift towards unplanted and planted mesocosm CLPP groupings [days 74-232]. 相似文献