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981.
982.
We investigated the dynamics of the SOS response induction and the frequency of reversions induced by the monofunctional alkylating compound N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli cells exposed to osmotic stress for 1 h. During the stress treatment of the wild-type cultures adapted and not adapted to the alkylating agent, the maximum SOS response values and induced reversion frequencies were recorded twice. The SOS response values and induced reversion frequencies remained unchanged during the whole period after attaining the maximum values in adapted and nonadapted cells carrying a mutation in the excision repair gene. Presumably, the SOS mutagenesis mechanisms are turned on in the cells with an inactivated excision repair system earlier than in wild-type cells. 相似文献
983.
984.
Wesselingh RA 《The New phytologist》2007,174(1):26-37
The standard method of measuring pollen limitation is to add pollen to a number of flowers, preferably to a whole plant, and to compare fruit and seed set with that of naturally pollinated flowers on other plants. In 25 yr of research, this method has yielded valuable data, but it is difficult to use in large plants. This has caused a bias in the available data towards smaller, herbaceous plants with relatively few flowers. I argue that, in order to widen our knowledge of how pollen limitation affects plants, we should go beyond whole-plant pollen addition and change our concept of how a flowering plant functions. The traditional method does not take into account the variation in and dynamics of resource allocation and pollen availability. The concept of integrated physiological units (IPUs) does, but, although it has been applied to pollination biology, it has not received the attention it deserves. I use this article to present its merits again, to propose a step-by-step methodology for studying pollen limitation, and to examine factors influencing possible plant strategies. 相似文献
985.
Effects of physical connection and genetic identity of neighbouring ramets on root-placement patterns in two clonal species 总被引:5,自引:0,他引:5
Root-placement patterns were examined in the clonal species Glechoma hederacea and Fragaria vesca when grown with different types of neighbours. Three different patterns were predicted as consequences of different types of interactions between roots: the avoidance pattern if root growth decreases in the presence of neighbouring roots; the intrusive pattern if root growth increases towards neighbouring roots; and the unresponsive pattern if root growth is unaffected by neighbouring roots. Experiments were conducted in which physical connection between ramets, and the genetic identity of neighbouring ramets, were manipulated. The patterns of distribution of entire root systems and elongation rates of individual roots were measured. Root systems and individual roots of G. hederacea avoided contact with roots of neighbouring ramets, irrespective of connection to the neighbour and its genetic or specific identity. In contrast, F. vesca roots grew equally towards and away from intraspecific ramet neighbours and their elongation was stimulated by contact with roots of G. hederacea ramets. These results demonstrate that root-placement patterns of plants grown with different types of neighbours vary between species, and suggest that factors additional to resource depletion could be involved in their development. 相似文献
986.
987.
Warming and free-air CO2 enrichment alter demographics in four co-occurring grassland species 总被引:2,自引:2,他引:0
Williams AL Wills KE Janes JK Vander Schoor JK Newton PC Hovenden MJ 《The New phytologist》2007,176(2):365-374
Species differ in their responses to global changes such as rising CO(2) and temperature, meaning that global changes are likely to change the structure of plant communities. Such alterations in community composition must be underlain by changes in the population dynamics of component species. Here, the impact of elevated CO(2) (550 micromol mol(-1)) and warming (+2 degrees C) on the population growth of four plant species important in Australian temperate grasslands is reported. Data collected from the Tasmanian free-air CO(2) enrichment (TasFACE) experiment between 2003 and 2006 were analysed using population matrix models. Population growth of Themeda triandra, a perennial C(4) grass, was largely unaffected by either factor but population growth of Austrodanthonia caespitosa, a perennial C(3) grass, was reduced substantially in elevated CO(2) plots. Warming and elevated CO(2) had antagonistic effects on population growth of two invasive weeds, Hypochaeris radicata and Leontodon taraxacoides, with warming causing population decline. Analysis of life cycle stages showed that seed production, seedling emergence and establishment were important factors in the responses of the species to global changes. These results show that the demographic approach is very useful in understanding the variable responses of plants to global changes and in elucidating the life cycle stages that are most responsive. 相似文献
988.
A range of traits, including metabolic costs, biomass allocation and seed reserves, may contribute to interspecific variation
in the shade tolerance of tree seedlings. In addition, shade tolerance may be affected by differential responses of species
to soil resource availability at low light. We used a custom-built whole-plant gas-exchange chamber to quantify instantaneous
whole-plant light compensation point (WPLCP) and to parameterize whole-plant daily C gain models for seedlings of eight temperate
deciduous tree species. We examined the relationship of WPLCP to growth, biomass allocation and gas-exchange under high and
low light and nutrient availabilities and compared it to WPCLP of naturally recruited saplings. For species showing a response,
both increased light and nutrient availability resulted in increased WPLCP. However, species’ responses to resource availability
did not correspond closely with shade tolerance as has generally been predicted. Variation in WPLCP within species was best
predicted by whole-plant dark respiration rates, leaf-level light compensation point and leaf mass per area. Among species,
seed size was a strong negative correlate of WPLCP, explaining 66% of the variation. Species with the lowest WPLCP maintained
lower growth rates across treatments but greater biomass in the low-light treatment compared with more light-demanding species.
These data suggest that a number of traits, in particular metabolic costs and seed size, contribute to WPLCP. However, gas-exchange-based
WPLCP was 1.5–3.5 times lower than corresponding growth-based field estimates of WPLCP, suggesting that other factors such
as biotic interactions or ontogenetic shifts in whole-plant light requirements may substantially increase species’ WPLCP under
natural conditions. 相似文献
989.
Plant annexins, Ca(2+)- and membrane-binding proteins, are probably implicated in the cellular response to stress resulting from acidification of cytosol. To understand how annexins can contribute to cellular ion homeostasis, we investigated the pH-induced changes in the structure and function of recombinant annexin AnnAt1 from Arabidopsis thaliana. The decrease of pH from 7.0 to 5.8 reduced the time of the formation of ion channels by AnnAt1 in artificial lipid membranes from 3.5 h to 15-20 min and increased their unitary conductance from 32 to 63 pS. These changes were accompanied by an increase in AnnAt1 hydrophobicity as revealed by hydrophobicity predictions, by an increase in fluorescence of 2-(p-toluidino)naphthalene-6-sulfonic acid (TNS) bound to AnnAt1 and fluorescence resonance energy transfer from AnnAt1 tryptophan residues to TNS. Concomitant lipid partition of AnnAt1 at acidic pH resulted in its partial protection from proteolytic digestion. Secondary structures of AnnAt1 determined by circular dichroism and infrared spectroscopy were also affected by lowering the pH from 7.2 to 5.2. These changes were characterized by an increase in beta-sheet content at the expense of alpha-helical structures, and were accompanied by reversible formation of AnnAt1 oligomers as probed by ultracentrifugation in a sucrose gradient. A further decrease of pH from 5.2 to 4.5 or lower led to the formation of irreversible aggregates and loss of AnnAt1 ionic conductance. Our findings suggest that AnnAt1 can sense changes of the pH milieu over the pH range from 7 to 5 and respond by changes in ion channel conductance, hydrophobicity, secondary structure of the protein and formation of oligomers. Further acidification irreversibly inactivated AnnAt1. We suggest that the pH-sensitive ion channel activity of AnnAt1 may play a role in intracellular ion homeostasis. 相似文献
990.
Metal hyperaccumulation is a striking trait exhibited by many plant species, but the evolutionary ecology of metal hyperaccumulation
is poorly understood. It has been widely hypothesized that metal hyperaccumulation evolved to protect plants from herbivory.
However, there is currently little evidence that metal hyperaccumulation enhances the fitness of plants in the presence of
herbivory. In this study, we conducted a multi-factor greenhouse experiment to examine the effects of two soil nickel concentrations
(unamended (0 μg/g) and nickel amended (600 μg/g)), and three levels of artificial damage (0, 10 and 50%) on the growth of
plants from two populations of Thlaspi montanum var. montanum. We observed a significant interaction between soil nickel and artificial damage. An a posteriori analysis of this interaction revealed that the presence of nickel significantly improved the ability of T. montanum to tolerate the negative effects of intense damage. Our results indicate that metal hyperaccumulation could benefit T. montanum by increasing its tolerance to damage. This study suggests that there is a potential for the evolution of metal hyperaccumulation
in response to intense herbivory on T. montanum. 相似文献