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61.
62.
Mg2 + dependent —adenosine triphosphatase activity has been studied in the muscle, brain, kidney and liver tissues of frog,Rana hexadactyla (Lesson) after sciatectomy and induced chronic ammonia stress. The enzyme activity decreased in the tissues of the denervated
frog. The activity of the enzyme increased in all the tissues of the normal and denervated frogs except in the denervated
muscle when ammonium lactate was infused intraperitoneally. 相似文献
63.
Incorporation of [14C]-phenylalanine and [14C]-methionine into cinnamon cuttings suggests that synthesis of eugenol from phenylalanine involves exchange of the terminal carbon in the side chain with that from a donor molecule such as methionine whereas synthesis of cinnamic aldehyde incorporates phenylalanine in toto. 相似文献
64.
An ammonia limited chemostat culture of Gymnodinium simplex (Lohm.) Kofoid & Swezy was perturbed with ammonia and fluctuations in the free intracellular amino acid pools were followed 80 min. The steady-state value of glutamate was 2.07 ± 10-15 mol cell-1 and of glutamine was 0.31 ± 10-15 mol cell-1. Five minutes after the perturbation, a substantial rise in glutamine was observed with a corresponding decrease in glutamate. This is considered a result of glutamine synthetase acting as the primary ammonia assimilating enzyme. The level of ammonia and the major free amino acids reached a maximum 10 min after the perturbation and then slowly decreased. 相似文献
65.
Laminaria longicrucis De la Pylaie took up exogenous nitrate under both summer and winter conditions. During July and August no NO3- was detected in the ambient water or in algal tissues although it was present in both in February. Discs (2.3 cm diam.) of thin blade tissue were incubated with NO3- at four temperatures, with and without illumination. Similar values Jor NO3- uptake were found for both summer and winter collected plants when measured in light at 0 C. An apparent K of 4–6 μM was recorded for both types of plants; the Vmax ranged from 7 to 10 μmol h-1 g-1 dry wt measured in ca. 1800 μW cm-2 of cool-white fluorescent light. Uptake rates at 5 C were 66%, and at 0 C 30% of those for controls run at 15 C. The alga scavenged NO3- from solutions <0.5 μM. Ammonia did not inhibit NO3- uptake. Antibiotic pretreatment reduced NO3- uptake by a maximum of 12%. Nitrite uptake was inhibited in proportion to the concentration of NO3- in the medium. 相似文献
66.
Jayalakshmi Kumpati 《Biochemical and biophysical research communications》1982,105(2):482-487
To investigate the role of phenylalanine and tryptophane as potential antisickling agents in intact human SS-red blood cells a liposomal transport system was employed to transfer phenyl-alanine or tryptophane into intact SS-red blood cells. Aromatic amino acids and short peptides containing phenylalanine have been demonstrated to increase the minimum gelling concentration and solubility of deoxy-hemoglobin S in aqueous solution. However, these compounds do not cross the red blood cell membrane under usual incubation conditions. Incorporation of phenylalanine or tryptophane into intact SS-red blood cells liposomal transport system markedly inhibited the sickling of deoxy-hemoglobin S. These findings raise the possibility that a nontoxic liposomal transport system which facilitates incorporation of antisickling agents into intact SS-RBC may have significant therapeutic implications in the treatment of sickle cell disease. 相似文献
67.
A number of inducible plant responses are believed to contribute to disease resistance. These responses include the hypersensitive reaction, phytoalexin synthesis, and the production of chitinase, glucanase, and hydroxyproline-rich glycoproteins. Because of the coordinate induction of these responses, it has been difficult to determine whether they are functional defense responses, and if they are, how they specifically contribute to disease resistance. Recent developments in molecular biology have provided experimental techniques that will reveal the specific contribution of each response to disease resistance. In this paper, we describe a strategy to determine if the hypersensitive reaction is a functional plant defense mechanism. 相似文献
68.
The influence of ammonia and lactate on cell growth, metabolic, and antibody production rates was investigated for murine hybridoma cell line 163.4G5.3 during batch culture. The specific growth rate was reduced by one-half in the presence of an initial ammonia concentration of 4 mM. Increasing ammonia levels accelerated glucose and glutamine consumption, decreased ammonia yield from glutamine, and increased alanine yield from glutamine. Although the amount of antibody produced decreased with increasing ammonia concentration, the specific antibody productivity remained relatively constant around a value of 0.22 pg/cell-h. The specific growth rate was reduced by one-half at an initial lactate concentration of 55 mM. Although specific glucose and glutamine uptake rates were increased at high lacatate concentration, they showed a decrease after making corrections for medium osmolarity. The yield coefficient of lactate from glucose decreased at high lactate concentrations. A similar decrease was observed for the ammonia yield coefficient from glutamine. At elevated lactate concentrations, specific antibody productivities increased, possibly due to the increase in medium osmolarity. The specific oxygen uptake rate was insensitive to ammonia and lactate concentrations. Addition of ammonia and lactate increased the calculated metabolic energy production of the cells. At high ammonia and lactate, the contribution of glycolysis to total energy production increased. Decreasing external pH and increasing ammonia concentrations caused cytoplasmic acidification. Effect of lactate on intracellular pH was insignificant, whereas increasing osmolarity caused cytoplasmic alkalinization. 相似文献
69.
Laboratory experiments have shown appreciable losses of ammonia after injection of anhydrous ammonia into dry and wet soils.
In this study losses of ammonia injected into a moist (tension 10 kPa), dry (tension 160 kPa) and a wet (tension 1.6 kPa)
sandy loam were measured under field conditions using wind tunnels. Losses were insignificant from a moist soil. However losses
from a dry and a wet soil were 20% and 50% of injected ammonia, respectively. From the dry soil, losses of gaseous ammonia
took place within the first hours after injection, which indicates a rapid transport through cracks and voids. From the wet
soil, 20% of the injected ammonia was lost more gradually between 6 h and 6 d. This indicates that upward movement of water
due to evaporation may be the cause of these ammonia losses which proceeded for longer periods. 相似文献
70.
菠萝黑心病是PPO催化氧化酚类物质形成褐色产物所致。低温或GA_3处理提高了PPO活性及其底物——儿茶酚、绿原酸和咖啡酸的含量,也导致了PAL活性增加;低温还使乙烯释放率增大。这些变化均有利于黑心病的发生和发展。 相似文献