全文获取类型
收费全文 | 4476篇 |
免费 | 280篇 |
国内免费 | 569篇 |
专业分类
5325篇 |
出版年
2024年 | 11篇 |
2023年 | 128篇 |
2022年 | 126篇 |
2021年 | 163篇 |
2020年 | 181篇 |
2019年 | 154篇 |
2018年 | 147篇 |
2017年 | 159篇 |
2016年 | 158篇 |
2015年 | 169篇 |
2014年 | 215篇 |
2013年 | 386篇 |
2012年 | 148篇 |
2011年 | 205篇 |
2010年 | 155篇 |
2009年 | 219篇 |
2008年 | 234篇 |
2007年 | 216篇 |
2006年 | 197篇 |
2005年 | 190篇 |
2004年 | 146篇 |
2003年 | 153篇 |
2002年 | 132篇 |
2001年 | 102篇 |
2000年 | 97篇 |
1999年 | 100篇 |
1998年 | 85篇 |
1997年 | 83篇 |
1996年 | 65篇 |
1995年 | 90篇 |
1994年 | 57篇 |
1993年 | 64篇 |
1992年 | 62篇 |
1991年 | 40篇 |
1990年 | 56篇 |
1989年 | 31篇 |
1988年 | 35篇 |
1987年 | 32篇 |
1986年 | 43篇 |
1985年 | 47篇 |
1984年 | 59篇 |
1983年 | 37篇 |
1982年 | 45篇 |
1981年 | 21篇 |
1980年 | 23篇 |
1979年 | 17篇 |
1978年 | 5篇 |
1977年 | 9篇 |
1976年 | 10篇 |
1974年 | 11篇 |
排序方式: 共有5325条查询结果,搜索用时 15 毫秒
91.
The degradation of phenol by Rhodococcus sp. P1 was studied in continuous culture systems. The organism could be adapted by slowly increasing concentration, step by step, up to 30.0 g · 1-1 phenol in the influent. The degradation rate reached values of about 0.3 g · g dry mass-1 ·h-1. Large step increases in phenol concentration and addition of further substrates (e.g., catechol) were tolerated up to a certain concentration. With increasing dilution rate and increasing inlet phenol concentration the stability of the system decreased. 相似文献
92.
Three strains of strictly anaerobic Gram-negative, non-sporeforming, motile bacteria were enriched and isolated from freshwater sediments with 1,3-propanediol as sole energy and carbon source. Strain OttPdl was a sulfate-reducing bacterium which grew also with lactate, ethanol, propanol, butanol, 1,4-butanediol, formate or hydrogen plus CO2, the latter only in the presence of acetate. In the absence of sulfate, most of these substrates were fermented to the respective fatty acids in syntrophic cooperation with Methanospirillum hungatei. Sulfur, thiosulfate, or sulfite were reduced, nitrate not. The other two isolates degraded propanediol only in coculture with Methanospirillum hungatei. Strain OttGlycl grew in pure culture with acetoin and with glycerol in the presence of acetate. Strain WoAcl grew in pure culture only with acetoin. Both strains did not grow with other substrates, and did not reduce nitrate, sulfate, sulfur, thiosulfate or sulfite. The isolates were affiliated with the genera Desulfovibrio and Pelobacter. The pathways of propanediol degradation and the ecological importance of this process are discussed. 相似文献
93.
Margareta Wandel Trond Berg Winnie Eskild Kaare R. Norum 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1982,721(4):469-477
The intracellular movement, following uptake of 125I-labelled denatured serum albumin into nonparenchymal liver cells, was followed by means of subcellular fractionation. Isolated nonparenchymal rat liver cells were prepared by means of differential centrifugation. The cells were homogenized in a sonifier and the cytoplasmic extract subjected to isopycnic centrifugation in a sucrose gradient. The intracellular movement of the labelled albumin was followed by comparing the distribution profile of radioactivity in the sucrose gradient with those of marker enzymes for plasma membrane and lysosomes. The distribution profiles for radioactivity after the cells had been exposed to the labelled denatured albumin for different time periods indicated that the radioactivity was first associated with subcellular fractions of lower modal densities than the lysosomes. With time of incubation the radioactivity moved towards higher densities. After prolonged incubations in the absence of extracellular labelled denatured albumin the radioactivity peak coincided with that of the lysosomal marker β-acetylglucosaminidase. When the cells were treated with the lysosomal inhibitor leupeptin, degradation of the labelled albumin was decreased, resulting in a massive intracellular accumulation of radioactivity. The radioactivity peak coincided with the peak of activity for the lysosomal marker β-acetylglucosaminidase, suggesting lysosomal degradation. 相似文献
94.
Tryptophan was found to be degraded in Saccharomyces cerevisiae mainly to tryptophol. Upon chromatography on DEAE-cellulose two aminotransferases were identified: Aromatic aminotransferase I was constitutively synthesized and was active in vitro with tryptophan, phenylalanine or tyrosine as amino donors and pyruvate, phenylpyruvate or 2-oxoglutarate as amino acceptors. The enzyme was six times less active with and had a twenty times lower affinity for tryptophan (K
m=6 mM) than phenylalanine or tyrosine. It was postulated thus that aromatic aminotransferase I is involved in vivo in the last step of tyrosine and phenylalanine biosynthesis. Aromatic aminotransferase II was inducible with tryptophan but also with the other two aromatic amino acids either alone or in combinations. With tryptophan as amino donor the enzyme was most active with phenylpyruvate and not active with 2-oxoglutarate as amino acceptor; its affinity for tryptophan was similar as for the other aromatic amino acids (K
m=0.2–0.4 mM). Aromatic aminotransferase II was postulated to be involved in vivo mainly in the degradation of tryptophan, but may play also a role in the degradation of the other aromatic amino acids.A mutant strain defective in the aromatic aminotransferase II (aat2) was isolated and its influence on tryptophan accumulation and pool was studied. In combination with mutations trp2
fbr, aro7 and cdr1-1, mutation aat2 led to a threefold increase of the tryptophan pool as compared to a strain with an intact aromatic aminotransferase II. 相似文献
95.
The apparent penetration activity of Schistosoma mansoni cercariae was quantified by means of an in vitro assay with a radioactively labeled Type I collagen gel. Both live cercariae and cercarial preacetabular gland secretions degraded the collagen. The addition of skin lipid or linoleic acid to the gel surface enhanced the degradation by live cercariae. 相似文献
96.
Summary The effects of coniferous monoculture on the distribution of C, Fe and Al in the upper horizons of brown ochreous earths of the Belgian Ardennes were investigated by comparing 5 soil profiles developed under an 80 year old spruce stand with another 5 profiles developed under a climactic broad-leaved forest (beechwood).Organic carbon, Fe and Al were extracted with 0.1N NaOH/Na-tetraborate solution buffered at pH 9.7: recent studies have shown that this extraction is particularly appropriate for the detection of incipient podzolisation in brown earths-brown podzolic soils intergrades.Even if most of the classic podzolisation indexes fail to illustrate differences, nevertheless our results show that fulvic acids and organo-ferric complexes are present in significantly greater amounts in the upper part of the cambic (B)1 horizon of the soils developed under conifers. Moreover, this podzolic tendancy is confirmed by the weathering patterns of the clay minerals in the A1(B) horizons developed under spruces, i.e. a more pronouced weathering of chloritic layers than those observed in the beechwood soil, with a correlative genesis of more abundant smectite-like minerals. One may therefore conclude that the change in the humus type (moder to mor) after the planting of spruce trees, has been sufficient, within the local climatic and edaphic context, to promote incipient podzolisation. 相似文献
97.
Analysis of Lepidium sativum seeds showed the presence of allyl, 2-phenethyl and benzyl glucosinolates, the first two being reported for the first time from this source. The effects of temperature, pH of the extraction medium and the length of time allowed for autolysis were assessed on the benzyl glucosinolate degradation products in seed extracts. In particulàr benzyl thiocyanate was not produced at higher temperatures but at ambient and lower temperatures it exceeded isothiocyanate. Nitrile was always the major product under the conditions studied, ever at pH levels as high as 7.4. Five new possible benzyl glucosinolate degradation products were detected and evidence is presented that benzaldehyde and benzyl alcohol could be secondary products formed thermally from isothocyanate and thiocyanate, respectively. Benzyl mercaptan and benzyl methyl sulphide also appear to be thermally produced. 相似文献
98.
Resting cells of Clostridium sticklandii took up thymine or uracil, when grown in a medium containing 40 mM serine and 20 mM thymine or uracil. The uptake was much lower, when the cells had been grown in a complex medium. Cell-free extracts from cells grown in the complex medium reduced the two bases to the dihydro compounds and decomposed dihydrothymine to -ureidoisobutyrate, as indicated by thin-layer chromatography. Uptake and degradation were stimulated by both NADH and NADPH. Further breakdown did not occur, as 14CO2 was not evolved from C-2-labelled thymine or uracil. The rates of pyrimidine uptake and breakdown of C. sticklandii were lower than those reported for C. sporogenes (Hilton et al., 1975). 相似文献
99.
Adenosine deaminase (EC 3.5.4.4) was found to occur in the extract of Azotobacter vinelandii, strain 0, and purified by heating at 65°C, fractionation with ammonium sulfate, DEAE-cellulose chromatography and gel filtration on Sephadex G-150. Purified adenosine deaminase was effectively stabilized by the addition of ethylene glycol. The molecular weight of the enzyme was estimated to be 66,000 by gel filtration on Sephadex G-150. The enzyme specifically attacked adenosine and 2-deoxyadenosine to the same extent, and formycin A to a lesser extent. The pH optimum of the enzyme was observed at pH 7.2. Double reciprocal plot of initial velocity versus adenosine concentration was concave upward, and Hill interaction coefficient was calculated to be 1.5, suggesting the allosteric binding of the substrate. ATP inhibited adenosine deaminase in an allosteric manner, whereas other nucleotides were without effect. The physiological significance of the enzyme was discussed in relation to salvage pathway of purine nucleotides. 相似文献
100.