Age dependent alterations in phospholipid composition of a saprophytic and a pathogenic strain of Nocardia

Nocardia polychromogenes (saprophytic) and Nocardia asteroides (pathogenic) showed characteristic patterns in changes of cellular lipids during growth. Total lipids and total phospholipids decreased with the age of the culture in the saprophytic strain, whereas in the pathogenic strain total lipids increased throughout the culture period and the total phospholipids decreased in the late stationary phase. The decrease in total phospholipids in saprophytic strain was reflected in the individual phosphatides. In the pathogenic strain, the phosphatidylinositomannoside content doubled in early stationary phase. Differences were observed in fatty acid composition of phosphatides at various stages of growth, but the ratio of saturated to unsaturated fatty acids remained unaltered.     

Mg2+ restores membrane potential in rat liver mitochondria deenergized by Ca2+ and phosphate movements

Cellular ornithine biosynthesis could be expected to play a significant role in putrescine formation and hence in growth. Two enzymes are involved in ornithine biosynthesis: arginase and transamidinase. These enzyme activities were studied in two human melanoma cell lines differing in their K_m of diamine oxidase for putrescine and in their tumorigenicity in nude mice. Arginase activity accounts for the majority of ornithine formed in the highly tumorigenic cell line, while the majority of ornithine is derived from transamidinase action in the poorly tumorigenic cell line, with concomitant formation of methyl guanidine, a potent inhibitor of diamine oxidase.     

Chromatin structure during the prereplicative phases in the life cycle of mammalian cells

The object of this study was to determine the kinetics of chromosome decondensation during the G₁ period of the HeLa cell cycle. HeLa cells synchronized in the G₁ period following the reversal of mitotic block were fused with Colcemid-arrested mitotic HeLa cells at 1.5, 3, 5, and 7 h after the reversal of N₂O block. The resulting prematurely condensed chromosomes (PCC) were classified into six categories depending on the degree of their condensation. The frequency of occurrence of each category was plotted as a function of time after mitosis. The results of this study indicate that the process of chromosome decondensation, initiated during the telophase of mitosis continues throughout the G₁ period without any interruption, thus the chromatin reaches an ultimate state of decondensation by the end of G₁ period, when DNA synthesis is initiated.     

Effects of metabolic inhibitors on cell lethality and mutation induction in Chinese hamster cells. I. Inhibitors of de novo purine synthesis and a comparison with the effects of caffeine

The effect of pre- and posttreatment incubation of UV-irradiated and ethyl methanesulphonate (EMS) treated cells with non-toxic concentrations of inhibitors of de novo purine synthesis (dnPS) on expression of potentially lethal and premutational damage at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in V79 cells has been examined. The concentrations of inhibitors used were shown to profoundly perturb de novo DNA synthesis, by measurements of [¹⁴C]formate uptake, and cell cycle progression by flow cytofluorimetry. Postincubation in 6-methyl mercaptopurine ribonucleoside (MMPR) usually but not invariably potentiated the cytotoxic effects of UV and EMS but azaserine (AZS) and methotrexate (MTX) were without effect. No effects on mutant frequencies were observed on posttreatment with any of these agents. Caffeine produced the least effect on dnPS, but invariably potentiated lethal damage. This potentiation of lethal damage is not mediated by dnPS inhibition as has been suggested for Chinese hamster ovary (CHO) cells.     

L'âge du complexe récifal des côtes de meuse entre verdun et commercy et la stratigraphie de l'oxfordien dans l'est du bassin de Paris

The question of the age of «Hauts de Meuse coral rag is studied again using new faunas of brachiopods and ammonites collected above, below and inside the coral rag. Ammonites fauna of the well known «Calcaires de Creuë is revised. Results obtained agree closely.The «Hauts de Meuse coral rag grew up during Middle Oxfordian time; and extend part of the Plicatilis zone (Antecedens Subzone) and the whole Transversarium Zone up to the basis of the Bifurcatus Zone, for the first time identified in Lorraine. So it span a short depositional time, about 1 MY, with a high deposition rate, the thickness of the coral rag being more than 100 meters, reaching 150 meters in Saint-Mihiel area. Towards SE, on the other side of the Marne Gap, the sudden change into basinal facies go with a reducing of thickness and deposition rate.Upper Oxfordian deposits show a relative uniformity of facies and thickness. At the top of Middle Oxfordian limestones, the age of the discontinuity and the Sequanian renewal of deposition is basal Bifurcatus Zone. Erosional renewal and terrigeneous deposits are a wide spread event by the beginning of Upper Oxfordian.In the paleontological part, species upon which are based new ages and interpretations are only figured, except the species new or with new paleontological data, which are described. Type specimen of Perisphinctes mosensisBayle, from the «Calcaires de Creuë, is described and photographically figured for the first time.     

Eine einfache methode zur erfassung der parameter von spitzwinkeligen gastropodengehäusen

The D.M. Raup & A. Michelson model of assessing shell-coiling constitutes a method which simplifies the measuring of spiral-coiling gastropod shells. With, this method the information is obtained by measuring the photograph of the object.The newly developed method has the advantage of dispensing with an exact shell orientation (horizontally located axis) and magnification.Instead of photographing the object with a camera the shell is placed upon photographic paper and in this manner a contact showing the shell outlines can be achieved by exposing and developing the print. Correct information is then obtained by means of the conversion factors (1–3) as outlined in this paper.     

Random recirculation of small T lymphocytes from thoracic duct lymph in the mouse

The migration of ⁵¹Cr-labeled nylon-wool separated mouse thoracic duct T cells has been followed in order to determine whether there is a circulation of small (nondividing) T cells through the small intestine. Approximately 6% of the injected dose of T-TDL localized in the small intestine (minus Peyer's patches). Experiments revealed that this gut-localizing cell population consisted almost entirely, if not exclusively, of lymphoblasts present in mouse T-TDL. When lymphoblasts and small lymphocytes from mouse T-TDL were separated by velocity sedimentation, and the migration of separated fractions was studied, we found large cells (66% blasts) migrated well to the gut but poorly to the lymph nodes, whereas small cells (2% blasts) showed minimal migration to the gut but localized randomly in lymph nodes and spleen. The in vivo distribution of small cells from T-TDL was similar to that of T-PLN. Furthermore, the recirculatory patterns of both ⁵¹Cr-labeled T-TDL and T-PLN were found to be identical as accessed by their rate of recovery in the thoracic duct lymph of recipient mice. These results support the notion that the vast majority of T-TDL and T-PLN are part of a common pool of recirculating T cells which recirculate randomly through lymph nodes and spleen and not the small intestine.     

Developpement,Croissance et fecondite de cladoceres en fonction de la temperature et du niveau trophique. Elevage experimental in situ dans deux etangs

Samples of Daphnia longispina, Ceriodaphnia quadrangula and Bosmina longirostris have been simultaneously reared in cages immersed in two fish ponds differing principally by their trophic status.Vital parameters such as duration of postembryonic stage, rate of growth of young and adults as well as fecundity were studied against environmental factors.The importance of food quantity, temperature and of their interaction on size, rate of growth and fecundity was investigated.The magnitude of the discrepancies between these parameters as measured under natural and laboratory conditions is stressed.

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Developpement, Croissance et fecondite de cladoceres en fonction de la temperature et du niveau trophique. Elevage experimental in situ dans deux etangs

Institut Royal des Sciences Naturelles de Belgique et Service de Biologie, Université de l'Etat à Mons     

Biochemical research on oogenesis. RNA accumulation during oogenesis of the dogfish Scyliorhinus caniculus

Four biochemical mechanisms have been shown to operate in the oocytes of amphibians and teleosts: (1) amplification of the 28 S and 18 S genes, (2) noncoordinate accumulation of 5 S RNA and 28 S + 18 S RNA, (3) storage of 5 S and transfer RNA made in excess by small oocytes within nucleoprotein particles, (4) expression of different 5 S genes in oocytes and somatic cells. We have tried to extend these observations to another group of vertebrates, i.e., selacians (Chondrichthya). Our data suggest that ribosomal gene amplification is low or absent in the oocytes of the dogfish Scyliorhinus caniculus. However, previtellogenic oocytes of this species accumulate more 5 S RNA than needed for ribosome assembly. Transfer and 5 S RNA present in small oocytes are probably not free in the cell sap. A substantial fraction of these RNAs sediments at 10 S when homogenates of immature ovaries are centrifuged in sucrose density gradients. In contrast to what we observed in amphibians and teleosts, 5 S RNA from ovaries of S. caniculus is identical in sequence to 5 S RNA from liver. Among the four mechanisms mentioned above, the second and probably the third one are used by the oocytes of S. caniculus. Mechanism (4) is absent in this species. No definitive conclusion can be drawn concerning mechanism (1), i.e., ribosomal gene amplification.