应用细胞工厂建立人用狂犬病疫苗连续培养多次收获工艺

目的为提高生产效率、增加原代地鼠肾细胞单产量及狂犬病病毒产量,建立人用狂犬病疫苗（地鼠肾细胞）连续培养多次收获工艺。方法选用12-14日龄SPF地鼠,无菌取肾经消化,制备成细胞悬液,分装到40层细胞工厂并培养细胞成单层;接种狂犬病病毒固定毒aG株,连续培养病毒并多次收获。分别对同一细胞批制备的多个单次病毒收获液的免疫原性、病毒滴度和地鼠肾细胞蛋白质含量进行检测。结果用40层细胞工厂培养原代地鼠肾细胞和狂犬病病毒,细胞接种浓度为1．0×10。～1．5x10。cells／mL,（36±1）℃培养72h成致密单层;按0．1MOI病毒接种,可进行6次收获病毒;多个单次病毒收获液病毒滴度均不低于6．0lgLD50／mL;免疫原性检查保护指数不低于100;地鼠肾细胞蛋白质残留量随着收获次数的增加而不断降低。结论用细胞工厂建立了人用狂犬病疫苗连续培养多次收获工艺,能显著提高地鼠。肾单产量,增加产能。     

工厂化生产海鲜菇菌包污染霉菌的鉴定及防治

对工厂化生产中海鲜菇菌包污染霉菌进行分离,根据霉菌的形态特征、培养性状及ITS序列分析,鉴定其为哈茨木霉、拟康氏木霉、脉孢霉、长枝木霉、黑曲霉、产红青霉和产黄青霉;在此基础上探讨了常用抑菌剂对霉菌的防治效果及对海鲜菇菌丝生长的影响。结果表明,质量浓度100 mg/L克霉灵对哈茨木霉、黑曲霉、产红青霉、产黄青霉、拟康氏木霉有强抑制作用,质量浓度100 mg/L多菌灵对长枝木霉、产红青霉、产黄青霉、拟康氏木霉有强抑制作用,二者对海鲜菇菌丝生长的抑制都比较弱。可为海鲜菇工厂化生产中污染霉菌的综合防治提供参考。     

原代地鼠肾细胞及狂犬病毒不同培养方式的研究

目的通过比较原代地鼠肾细胞在转瓶、微载体、细胞工厂的3种培养方式的培养效果,为原代地鼠肾细胞选择一种易扩大规模、培养高质量细胞的培养方式,进而提高狂犬病毒的产量。方法消化取得的细胞悬液分别在转瓶、微载体、细胞工厂中培养,通过显微镜观察细胞形态、计数等结果比对培养的差别。结果细胞工厂可以很好地培养原代地鼠肾细胞和狂犬病毒;而细胞在微载体上贴附性差,生长不好。结论实验结果表明细胞工厂可以取代转瓶,用于大规模培养原代地鼠肾细胞扩大狂犬病毒的产量。     

Bacterial Diversity and Biogeochemical Processes of Oil-Contaminated Groundwater,Baoding, North China

A total of 43 groundwater samples were collected from 9 multimonitoring wells at a petrochemical site, Baoding City, North China, from June 2008 to December 2009 to investigate the biogeochemical processes and/or bacterial conmmunity using both culture-dependent and -independent methods. The results showed that aromatic hydrocarbons and chlorinated hydrocarbons were the major pollutants in the groundwater. Denitrification and iron reduction might be the main biogeochemical processes in the aquifers at this site, which seemed to transform from denitrification-dominated to iron reduction-dominated in some sections. Denaturing gradient gel electrophoresis (DGGE) revealed that the dominant bacterial groups of the groundwater were related to some oil-degrading bacteria, which can grow under denitrifying, iron-reducing and sulfate-reducing anaerobic conditions. In some serious contaminated groundwater niches, there might be sulfur cycles, as sulfur oxidizer was also abundant, which was further confirmed by 16S rRNA gene cloning analysis. The operational taxonomic units (OTUs) that highly related to Pseudomonas sp., Hydrogenophaga sp., Sphingomonas sp., Ferribacterium sp. and Sulfuricurvum Kujiense etc. were predominant in the groundwater contaminated by chlorinated hydrocarbons (CHCs), benzene, toluene, ethylbenzene, and xylenes (BTEX) and/or polycyclic aromatic hydrocarbons (PAHs), respectively. Biodiversity seemed to be undermined by oil contamination, and varied with seasons. The bacterial community in the contaminated groundwater was largely determined by the groundwater geochemistry.     

Arsenic exposure levels of petrochemical workers in three workplace settings in Rayong Province,Thailand

Background: Crude oil and natural gas are often contaminated with arsenic. As a carcinogen, arsenic contamination in the workplace is of concern, particularly when urinary arsenic levels are higher than the standard. The aim of this study was to identify exposure sources of arsenic among petrochemical workers. Methods: A total of 188 operators and 30 office workers participated in the study. Ninety-three workplace air samples, three main meals in five consecutive days, and drinking water were collected from each participant. Urine was collected at the end of the day after the last food sample was collected from each subject. Urine samples where arsenic concentration exceeded 100 mg/L were further analyzed to identify species. Results: The average arsenic concentrations in operators' and office workers' food and urine were 0.55 ± 1.00 and 0.49 ± 0.67 mg/kg; and 76.43 ± 107.36 and 149.92 ± 200.28 mg/L, respectively. The arsenic concentrations in air and water were well below their standards. The urinary arsenic correlated well with arsenic in the food but not in the air and water. Conclusion: Occupational exposure to arsenic among operators and office workers was lower than 1% TLV (Threshold limit value) and did not differ significantly. The major source of arsenic exposure Q2 was food.     

ECG changes in factory workers exposed to 27.2 MHz radiofrequency radiation

To research the effect of 27.2 MHz radiofrequency radiation on electrocardiograms (ECG), 225 female workers operating radiofrequency machines at a shoe factory were chosen as the exposure group and 100 female workers without exposure from the same factory were selected as the control group. The 6 min electric field strength that the female workers were exposed to was 64.0 ± 25.2 V/m (mean ± SD), which exceeded 61 V/m, the International Commission on Non‐Ionizing Radiation Protection reference root mean square levels for occupational exposure. A statistical difference was observed between the exposed group and the control group in terms of the rate of sinus bradycardia (χ² = 11.48, P = 0.003). When several known risk factors for cardiovascular disease were considered, including smoking, age, alcohol ingestion habit, and so on, the exposure duration was not an effective factor for ECG changes, sinus arrhythmia, or sinus bradycardia according to α = 0.05, while P = 0.052 for sinus arrhythmia was very close to 0.05. We did not find any statistical difference in heart rate, duration of the QRS wave (ventricular depolarization), or corrected QT intervals (between the start of the Q wave and end of the T wave) between the exposed and control groups. Occupational exposure to radiofrequency radiation was not found to be a cause of ECG changes after consideration of the confounding factors. Bioelectromagnetics 34:285–290, 2013. © 2012 Wiley Periodicals, Inc.     

牛肾细胞在两种不同载体中培养效果的比较

目的通过牛肾细胞在两种不同载体中培养效果的比较,为牛肾细胞在细胞工厂中规模化生产提供真实的、有力的支持。方法不同代次牛肾细胞在两种载体中经过相同培养条件进行培养。结果实验中原代牛肾细胞在细胞工厂接种密度为5.5×104/cm2左右,在15 L转瓶接种密度为9.0×104/cm2左右。一代牛肾细胞在细胞工厂接种密度为6.5×104/cm2左右,在15 L转瓶接种密度为10×104/cm2左右。二代牛肾细胞在细胞工厂接种密度为7.0×104/cm2左右,在15 L转瓶接种密度为14×104/cm2左右。两种载体中牛肾细胞生长状况均能达到培养要求。结论细胞工厂能在有限的空间内利用最大限度的培养表面培养牛肾细胞,不仅节约了传代前的细胞用量,而且提高了培养后的细胞产量。