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11.
Cellular iron homeostasis is critical for survival and growth. Bacteria employ a variety of strategies to sequester iron from the environment and to store intracellular iron surplus that can be utilized in iron‐restricted conditions while also limiting the potential for the production of iron‐induced reactive oxygen species (ROS). Here, we report that membrane‐derived oligosaccharide (mdo) glucan, an intrinsic component of Gram‐negative bacteria, sequesters the ferrous form of iron. Iron‐binding, uptake, and localization experiments indicated that both secreted and periplasmic β‐(1,2) ‐ glucans bind iron specifically and promote growth under iron‐restricted conditions. Xanthomonas campestris and Escherichia coli mutants blocked in the production of β‐(1,2) ‐ glucan accumulate low amounts of intracellular iron under iron‐restricted conditions, whereas they exhibit elevated ROS production and sensitivity under iron‐replete conditions. Our results reveal a critical role of glucan in intracellular iron homeostasis conserved in Gram‐negative bacteria. 相似文献
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Oliver Hecht Mireille Vankemmelbeke Amit Sharma Anne Marie Krachler Nicholas G Housden Katie J Lilly Richard James Geoffrey R Moore Colin Kleanthous 《The EMBO journal》2009,28(18):2846-2857
The Tol system is a five‐protein assembly parasitized by colicins and bacteriophages that helps stabilize the Gram‐negative outer membrane (OM). We show that allosteric signalling through the six‐bladed β‐propeller protein TolB is central to Tol function in Escherichia coli and that this is subverted by colicins such as ColE9 to initiate their OM translocation. Protein–protein interactions with the TolB β‐propeller govern two conformational states that are adopted by the distal N‐terminal 12 residues of TolB that bind TolA in the inner membrane. ColE9 promotes disorder of this ‘TolA box’ and recruitment of TolA. In contrast to ColE9, binding of the OM lipoprotein Pal to the same site induces conformational changes that sequester the TolA box to the TolB surface in which it exhibits little or no TolA binding. Our data suggest that Pal is an OFF switch for the Tol assembly, whereas colicins promote an ON state even though mimicking Pal. Comparison of the TolB mechanism to that of vertebrate guanine nucleotide exchange factor RCC1 suggests that allosteric signalling may be more prevalent in β‐propeller proteins than currently realized. 相似文献
13.
Chun-Yi Hu Shu-Ling Hsieh Jaang J. Wang Yo-Chia Chen Liang-Ping Lin 《World journal of microbiology & biotechnology》2006,22(8):873-880
The symbiotic roles of rhizobia with legumes have been extensively made use of in the agricultural field. An unresolved question about the infection process is the nature of the Rhizobium enzymes that degrade the plant cell wall. For the bacteria in the plant root-hair wall, carboxymethylcellulase (EC 3.2.1.4) could be one of the key enzymes in the early symbiotic process. Electron microscopic immunogold labeling experiments were performed with ultrathin sections of Sinorhizobium fredii CCRC15769. The results preliminarily indicated that antigenic determinants of membrane-bound carboxymethylcellulase are exposed, at the outside of the cytoplasmic membrane, to the periplasmic space; immuno-gold particles with 10nm diameter were hardly ever observed in the cytoplasm and other spaces. 相似文献
14.
Thomas J. Mansell Stephen W. Linderman Adam C. Fisher Matthew P. DeLisa 《Protein science : a publication of the Protein Society》2010,19(5):1079-1090
An array of genetic screens and selections has been developed for reporting protein folding and solubility in the cytoplasm of living cells. However, there are currently no analogous folding assays for the bacterial periplasm, despite the significance of this compartment for the expression of recombinant proteins, especially those requiring important posttranslational modifications (e.g., disulfide bond formation). Here, we describe an engineered genetic selection for monitoring protein folding in the periplasmic compartment of Escherichia coli cells. In this approach, target proteins are sandwiched between an N‐terminal signal recognition particle (SRP)‐dependent signal peptide and a C‐terminal selectable marker, TEM‐1 β‐lactamase. The resulting chimeras are localized to the periplasmic space via the cotranslational SRP pathway. Using a panel of native and heterologous proteins, we demonstrate that the folding efficiency of various target proteins correlates directly with in vivo β‐lactamase activity and thus resistance to ampicillin. We also show that this reporter is useful for the discovery of extrinsic periplasmic factors (e.g., chaperones) that affect protein folding and for obtaining folding‐enhanced proteins via directed evolution. Collectively, these data demonstrate that our periplasmic folding reporter is a powerful tool for screening and engineering protein folding in a manner that does not require any structural or functional information about the target protein. 相似文献
15.
Rageia Elfageih Alexandros Karyolaimos Grant Kemp Jan‐Willem de Gier Gunnar von Heijne Renuka Kudva 《Protein science : a publication of the Protein Society》2020,29(10):2028-2037
Cotranslational protein folding studies using Force Profile Analysis, a method where the SecM translational arrest peptide is used to detect folding‐induced forces acting on the nascent polypeptide, have so far been limited mainly to small domains of cytosolic proteins that fold in close proximity to the translating ribosome. In this study, we investigate the cotranslational folding of the periplasmic, disulfide bond‐containing Escherichia coli protein alkaline phosphatase (PhoA) in a wild‐type strain background and a strain background devoid of the periplasmic thiol: disulfide interchange protein DsbA. We find that folding‐induced forces can be transmitted via the nascent chain from the periplasm to the polypeptide transferase center in the ribosome, a distance of ~160 Å, and that PhoA appears to fold cotranslationally via at least two disulfide‐stabilized folding intermediates. Thus, Force Profile Analysis can be used to study cotranslational folding of proteins in an extra‐cytosolic compartment, like the periplasm. 相似文献
16.
《Journal of molecular biology》2021,433(15):167047
In Gram-negative bacteria, periplasmic domains in inner membrane proteins are cotranslationally translocated across the inner membrane through the SecYEG translocon. To what degree such domains also start to fold cotranslationally is generally difficult to determine using currently available methods. Here, we apply Force Profile Analysis (FPA) – a method where a translational arrest peptide is used to detect folding-induced forces acting on the nascent polypeptide – to follow the cotranslational translocation and folding of the large periplasmic domain of the E. coli inner membrane protease LepB in vivo. Membrane insertion of LepB’s two N-terminal transmembrane helices is initiated when their respective N-terminal ends reach 45–50 residues away from the peptidyl transferase center (PTC) in the ribosome. The main folding transition in the periplasmic domain involves all but the ~15 most C-terminal residues of the protein and happens when the C-terminal end of the folded part is ~70 residues away from the PTC; a smaller putative folding intermediate is also detected. This implies that wildtype LepB folds post-translationally in vivo, and shows that FPA can be used to study both co- and post-translational protein folding in the periplasm. 相似文献
17.
Wipa Chungjatupornchai Sirirat Fa-aroonsawat 《Journal of microbiology (Seoul, Korea)》2009,47(2):187-192
The translocation of proteins to cyanobacterial cell envelope is made complex by the presence of a highly differentiated membrane
system. To investigate the protein translocation in cyanobacterium Synechococcus PCC 7942 using the truncated ice nucleation protein (InpNC) from Pseudomonas syringae KCTC 1832, the green fluorescent protein (GFP) was fused in frame to the carboxyl-terminus of InpNC. The fluorescence of
GFP was found almost entirely as a halo in the outer regions of cells which appeared to correspond to the periplasm as demonstrated
by confocal laser scanning microscopy, however, GFP was not displayed on the outermost cell surface. Western blotting analysis
revealed that InpNC-GFP fusion protein was partially degraded. The N-terminal domain of InpNC may be susceptible to protease
attack; the remaining C-terminal domain conjugated with GFP lost the ability to direct translocation across outer membrane
and to act as a surface display motif. The fluorescence intensity of cells with periplasmic GFP was approximately 6-fold lower
than that of cells with cytoplasmic GFP. The successful translocation of the active GFP to the periplasm may provide a potential
means to study the property of cyanobacterial periplasmic substances in response to environmental changes in a non-invasive
manner. 相似文献
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大肠杆菌周质和外膜蛋白的定位 总被引:1,自引:0,他引:1
大肠杆菌周质和外膜蛋白发挥功能必须首先到达其特定亚细胞分区.大肠杆菌通过一系列与蛋白质分泌有关的蛋白(Sec蛋白)将周质和外膜蛋白转运至内膜.在切除了信号肽后,与周质蛋白的定位不同的是,外膜蛋白的最终定位还需要其他因子的协助.外膜蛋白的定位近来认为是以周质作为中介的. 相似文献
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