Analysis of quantitative trait loci affecting chlorophyll content of rice leaves in a double haploid population and two backcross populations

Chlorophyll content, one of the most important physiological parameters related to plant photosynthesis, is usually used to predict yield potential. To map the quantitative trait loci (QTLs) underlying the chlorophyll content of rice leaves, a double haploid (DH) population was developed from an indica/japonica (Zhenshan 97/Wuyujing 2) crossing and two backcross populations were established subsequently by backcrossing DH lines with each of their parents. The contents of chlorophyll a and chlorophyll b were determined by using a spectrophotometer to directly measure the leaf chlorophyll extracts. To determine the leaf chlorophyll retention along with maturation, all measurements were performed on the day of heading and were repeated 30 days later. A total of 60 QTLs were resolved for all the traits using these three populations. These QTLs were distributed on 10 rice chromosomes, except chromosomes 5 and 10; the closer the traits, the more clustering of the QTLs residing on common rice chromosomal regions. In general, the majority of QTLs that specify chlorophyll a content also play a role in determining chlorophyll b content. Strangely, chlorophyll content in this study was found mostly to be lacking or to have a negative correlation with yield. In both backcross F₁ populations, overdominant (or underdominant) loci were more important than complete or partially dominant loci for main-effect QTLs and epistatic QTLs, thereby supporting previous findings that overdominant effects are the primary genetic basis for depression in inbreeding and heterosis in rice.     

大豆小区产量及其相关性状QTL间的上位性和环境互作效应

以晋豆23栽培大豆(Glycine max)为母本、灰布支黑豆(ZDD2315,半野生大豆)为父本衍生出447个RIL群体,通过构建SSR遗传图谱及利用混合线性模型分析方法,对2年大豆小区产量及主要植物学性状进行QTL定位,并作加性效应、加性×加性上位互作效应及环境互作效应分析。结果显示,共检测到12个与小区产量、单株粒重、单株茎重、单株粒茎比、有效分枝、主茎节数、株高和结荚高度相关的QTL,分别位于A1、A2、H_1、I、J_2和M连锁群上。其中小区产量、株高、单株粒重、有效分枝和主茎节数均表现为遗传正效应,即增加其性状的等位基因来源于母本晋豆23。同时,检测到11对影响小区产量、单株粒重、单株茎重、株高和结荚高度的加性×加性上位互作效应及环境互作效应的QTL,发现22个QTL与环境存在互作。实验结果表明,上位效应和QE互作效应对大豆小区产量及主要农艺性状的遗传影响很大。进行大豆分子标记辅助育种时,既要考虑效应起主要作用的QTL,又要注重上位性QTL,这样有利于性状的稳定表达和遗传。     

Warming mediates the relationship between plant nutritional properties and herbivore functional responses

Quantifying the per capita effects of invasive alien species is crucial for assessing their ecological impact. A major challenge to risk assessment of invasive species was to understand the factors that cause per capita effects to vary in different ecological contexts, particularly in a warming world. By conducting functional response experiments, we estimated the per capita effects (attack rate and maximum feeding rate) of an invasive herbivorous snail, Pomacea canaliculata, toward ten host plant species. We tested whether variation in these effects is related to plant nutritional and physical properties (total N and dry matter content (DMC)) and examined how increasing temperature can shift these relationships. We observed stronger per capita effects (i.e., higher attack rate and maximum feeding rate) by the snail on plants with higher total N, but no direct relationship was found with DMC. A significant interaction effect of total N and DMC on the attack rate indicated that DMC probably adjusted the feeding indirectly. Warmer temperatures reduced correlations between snail functional responses and host plant nutritional properties (total N) by increasing maximum feeding rate for plants of low nutrition, but there was no such effect on attack rates. However, given the nonreplacement design used in our study, the nonsignificant effect of temperature on the attack rate should be caveated. Our result suggests that characterizing the per capita effects of herbivores using functional responses can reveal the mechanisms by which climate change may alter herbivore–plant interactions and, thus, the ecological impacts of introduced herbivores.     

Phylogenetic structural equation modelling reveals no need for an ‘origin’ of the leaf economics spectrum

The leaf economics spectrum (LES) is a prominent ecophysiological paradigm that describes global variation in leaf physiology across plant ecological strategies using a handful of key traits. Nearly a decade ago, Shipley et al. (2006) used structural equation modelling to explore the causal functional relationships among LES traits that give rise to their strong global covariation. They concluded that an unmeasured trait drives LES covariation, sparking efforts to identify the latent physiological trait underlying the ‘origin’ of the LES. Here, we use newly developed phylogenetic structural equation modelling approaches to reassess these conclusions using both global LES data as well as data collected across scales in the genus Helianthus. For global LES data, accounting for phylogenetic non‐independence indicates that no additional unmeasured traits are required to explain LES covariation. Across datasets in Helianthus, trait relationships are highly variable, indicating that global‐scale models may poorly describe LES covariation at non‐global scales.     

Is urban green space per capita a valuable target to achieve cities’ sustainability goals? Romania as a case study

Urban green infrastructure is considered to be a key element in improving quality of life and creating an appropriate framework for sustainable cities. The most used quantitative indicator to assess urban green infrastructure is urban green space (UGS) per capita. This papeŕs aim is to analyze whether UGS is a valuable target to achieve cities’ sustainability goals. We used Romania as a case study, which should meet the target of 26 m² of UGS per inhabitants in all cities. Aerial images were used to extract the surface of the UGS categories for a sample of 38 cities in Romania. We compared our data with three other databases (National Institute of Statistics, Environmental Protection Agencies and Urban Atlas) to check for differences. To understand the contribution of geographic and socio-economic factors to the dynamic of the UGS per capita in Romania’s cities, we used a multiple linear regression. To identify differences between cities in terms of their proportions of UGS, multiple correspondence analysis was performed. We found significant differences between the surface of UGS reported by the administrative offices and that resulting from the spatial analysis. The process of reporting UGS data currently has methodological and perspective shortcomings. Moreover, the density of the built-up space, the proximity to major transport infrastructure, the cities’ founding period and the geomorphology criteria are important predictors for the UGS. The target of 26 m² of green area per inhabitant in all Romanian cities is not feasible and it should consider the cities’ characteristics, if you want to achieve sustainability goals. Urban green planning should focus more on the development of urban green infrastructure models that are adapted to each type of urban area.     

Effect of environmental,economic and health factors on CoVid-19 transmission

The severe acute respiratory syndrome (SARS) is affected by meteorological parameters such as temperature and humidity. It is also observed that people having asthma are at risk for SARS. Therefore, it is of interest to report the effect of environmental, economic, and health factors on the spread of CoVid-19. We used data reporting CoVid-19 cases from 24 cities in eight different countries for this analysis. Data was analyzed using multiple linear regressions between these parameters. Data shows that temperature has effects on CoVid-19. A one-degree rise in temperature causes a -0.19 decrease in CoVid-19 cases per million people (log natural value per million populations). The effect of humidity is not significant at a p value of 0.26. Moreover, one-unit increase in asthma and GDP cases per million people show 0.06 and 0.46 increases in CoVid-19 cases, respectively.     

Electron transport chains and bioenergetics of respiratory nitrogen metabolism in Wolinella succinogenes and other Epsilonproteobacteria

Recent phylogenetic analyses have established that the Epsilonproteobacteria form a globally ubiquitous group of ecologically significant organisms that comprises a diverse range of free-living bacteria as well as host-associated organisms like Wolinella succinogenes and pathogenic Campylobacter and Helicobacter species. Many Epsilonproteobacteria reduce nitrate and nitrite and perform either respiratory nitrate ammonification or denitrification. The inventory of epsilonproteobacterial genomes from 21 different species was analysed with respect to key enzymes involved in respiratory nitrogen metabolism. Most ammonifying Epsilonproteobacteria employ two enzymic electron transport systems named Nap (periplasmic nitrate reductase) and Nrf (periplasmic cytochrome c nitrite reductase). The current knowledge on the architecture and function of the corresponding proton motive force-generating respiratory chains using low-potential electron donors are reviewed in this article and the role of membrane-bound quinone/quinol-reactive proteins (NapH and NrfH) that are representative of widespread bacterial electron transport modules is highlighted. Notably, all Epsilonproteobacteria lack a napC gene in their nap gene clusters. Possible roles of the Nap and Nrf systems in anabolism and nitrosative stress defence are also discussed. Free-living denitrifying Epsilonproteobacteria lack the Nrf system but encode cytochrome cd₁ nitrite reductase, at least one nitric oxide reductase and a characteristic cytochrome c nitrous oxide reductase system (cNosZ). Interestingly, cNosZ is also found in some ammonifying Epsilonproteobacteria and enables nitrous oxide respiration in W. succinogenes.     

Interaction of the Thermoplasma acidophilum A1A0-ATP synthase peripheral stalk with the catalytic domain

The peripheral stalk of the archaeal ATP synthase (A₁A₀)-ATP synthase is formed by the heterodimeric EH complex and is part of the stator domain, which counteracts the torque of rotational catalysis. Here we used nuclear magnetic resonance spectroscopy to probe the interaction of the C-terminal domain of the EH heterodimer (E_CT1H_CT) with the N-terminal 23 residues of the B subunit (B_NT). The data show a specific interaction of B_NT peptide with 26 residues of the E_CT1H_CT domain, thereby providing a molecular picture of how the peripheral stalk is anchored to the A₃B₃ catalytic domain in A₁A₀.

Structured summary

MINT-7260681: Hct (refseq:NP_393485), Ect1 (uniprotkb:Q9HM68) and Bnt (uniprotkb:Q9HM64) physically interact (MI:0915) by nuclear magnetic resonance (MI:0077)     

Egg Laying Capacity of Haplorchis taichui (Digenea: Heterophyidae) in Humans

Quantitative fecal egg counts represented as the number of eggs per gram of feces (EPG) are generally a reliable parameter to estimate the worm burden of intestinal and hepatic parasitoses. Although Haplorchis taichui (Digenea: Heterophyidae) is one of the most common minute human intestinal flukes, little is known about the relationship between EPG and the actual worm burden in patients or the severity of the disease. In the present study, fecal samples were collected from 25 villagers in northern Thailand before and after praziquantel treatment. The EPG values of each participant were determined by the modified cellophane thick smear method, and adult worms were collected from the whole stool after the treatment. Eggs per day per worm (EPDPW) of H. taichui were estimated 82 from egg counts and expelled worms. The EPG was not well correlated with the worm burden, and a reverse correlation was observed between the EPDPW and the worm burden.     

红松不同苗龄幼苗叶性状对温度和光照变化的响应

通过分析不同苗龄红松幼苗在控制温度和光照条件下叶性状的变化差异,以及各控制因素交互作用对红松幼苗叶性状的影响程度和红松叶性状的线性相关关系,探讨红松幼苗叶性状响应气候环境因素变化的特征和所采取的物质分配上的变化策略。实验采取人工气候箱培养红松幼苗,设置3组温度为10、20、30℃,每组温度下设置有效光照辐射4组:45.3、90.5、181.1、271.6(μmol m~(-2)s~(-1)),每组观测苗龄1—4a。观测性状为:比叶重(LMA)、单位面积光合能力(A_(area))、单位质量叶氮含量(N_(mass))和单位质量叶磷含量(P_(mass))。结果显示:(1)红松幼苗的LMA、A_(area)、N_(mass)、P_(mass)受到温度、光照和苗龄的影响,各控制组叶性状间差异显著(P0.05);(2)温度、光照和苗龄对红松幼苗叶性状的交互作用效应显著(P0.05),影响程度依次为:苗龄温度温度×光照×苗龄光照光照×苗龄温度×光照温度×苗龄;(3)LMA与温度和苗龄呈正相关性,A_(area)与苗龄呈正相关性,与温度呈负相关性,N_(mass)与温度、光照、苗龄、LMA和A_(area)均呈负相关性,P_(mass)与温度、N_(mass)呈正相关性而与苗龄和LMA呈负相关性。通过结果分析可以得出以下结论:(1)红松幼苗叶性状对温度、光照和苗龄3个因素均有明显响应但响应程度不一,影响性状差异的主要因素是温度和苗龄,光照在其温度和苗龄二者的作用基础上对性状产生影响;(2)红松幼苗叶性状之间的线性关系实际反映的是对温度、光照和苗龄响应的结果,叶性状间的密切联系反映出叶片在响应环境因素时所采取的分配策略的调整,其目的是维持植物体内同化作用和异化作用的平衡,确保红松幼苗稳定生长。