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31.
Yoshiaki Nakahara Masanao Matsui Kenji Mori 《Bioscience, biotechnology, and biochemistry》2013,77(11):2243-2245
A novel synthetic method of 3-substituted furans was developed and syntheses of perillen and dendrolasin are described. 相似文献
32.
Phenolase activity in spinach leaves homogenates depends on the stage of development of leaves and on the kind of homogenization procedure. Under constant experimental conditions it is low in non-senescent leaves. With the onset of senescence there is a 15–20-fold increase in soluble activity in the supernatants of broken chloroplasts as well as an increase in activation of latent phenolase in fractions containing thylakoids. This rise in activity is due to an increase in particular multiple forms, differing for supernatants and membrane sediments. Phenolase from spinach lacks monophenolase and laccase activities. 相似文献
33.
The O-diphenol-oxygen-oxidoreductase of Agaricus bisporus: Activity and multiple forms during ageing
Peter W. Goodenough 《Phytochemistry》1978,17(4):633-636
Mushroom o-diphenol oxidase was separated into multiple forms by isoelectric focusing. Three major bands, as opposed to the four isoenzymes previously found, were separated over the pH range 3.5–9.5. A fourth form was obtained when the pH range was narrowed to 5.0–8.0. Changes in the enzyme activity were investigated during post-harvest ageing at different temperatures. Rapid ageing using tissue discs with or without inhibitors of protein synthesis showed that an increase in activity of the enzyme took place during this time, but was prevented by actinomycin D and 6 methyl purine. 相似文献
34.
Analysis of leaf canopy overtopping relationships was carried out using a non-destructive point quadrat method followed by a destructive stratified harvest of the above-ground phytomass in Dutch chalk grasslands with different management: summer sheep grazing and annual autumn mowing. The two methods of analysis are compared: e.g. relative leaf overtopping can be recorded by the point quadrat method but it is obscured in vertical vegetation profiles based on stratified phytomass distribution. However the stratified harvest method describes the relationship between canopy phytomass and light microclimate, recorded by measuring Photosynthetically Active Radiation (PAR) at different heights in the vegetation. Plant growth form during peak standing crop is of greater significance than Raunkiaerian life form in determining structure of chalk grassland vegetation. In annually mown grasslands, the tall graminoid growth form, shown by Brachypodium pinnatum, reduces PAR in the lower canopy and suppresses most other growth forms except those which can reach the higher canopy levels, e.g. clumped herbs such as Origanum vulgare. In contrast, grazing can result in a reduction of dominance from the tall graminoids and reduction of the abundance of taller, grazing-sensitive herbs, e.g. clumped and climbing herbs such as Origanum and Lathyrus pratensis, and an increase in grazing-tolerant species, e.g. smaller rosette herb growth forms, e.g. Leontodon hispidus and shorter rhizomatous or stoloniferous graminoids, e.g. Carex flacca and Briza media. Changes in both the overtopping hierarchy at the peak of the growing season and the intensity of overtopping in the course of a growing season are of conclusive importance in determining the relative abundance of species in the vegetation. 相似文献
35.
Jia An Xiang Wang Yajiang Jing Jianping Huang Qilong Wang Gang Zhang Jing Gao Liang Peng Wenli Huang Yonggang Yan 《Phyton》2023,92(5):1405-1420
Although Platycodon grandiflorum (Jacq.) A.DC. is a renowned medicine food homology plant, reports of excessive cadmium (Cd) levels are common, which affects its safety for clinical use and food consumption. To enable its Cd levels to be regulated or reduced, it is necessary to first elucidate the mechanism of Cd uptake and accumulation in the plant, in addition to its detoxification mechanisms. This present study used inductively couple plasma-mass-spectrometry to analyze the subcellular distribution and chemical forms of Cd in different tissues of P. grandiflorum. The experimental results showed that Cd was mainly accumulated in the roots [predominantly in the cell wall (50.96%–61.42%)], and it was found primarily in hypomobile and hypotoxic forms. The proportion of Cd in the soluble fraction increased after Cd exposure, and the proportion of insoluble phosphate Cd and oxalate Cd increased in roots and leaves, with a higher increase in oxalate Cd. Therefore, it is likely that root retention mechanisms, cell wall deposition, vacuole sequestration, and the formation of low mobility and low toxicity forms are tolerance strategies for Cd detoxification used by P. grandiflorum. The results of this study provide a theoretical grounding for the study of Cd accumulation and detoxification mechanisms in P. grandiflorum, and they can be used as a reference for developing Cd limits and standards for other medicine food homology plants. 相似文献
36.
37.
Berberine exerts an anti-adipogenic activity that is associated with the down-regulation of C/EBPα and PPARγ. Stimulation of AMP-activated kinase (AMPK) caused by inhibition of mitochondrial respiration has been suggested to underlie such molecular regulation. In the present study, we show that berberine up-regulated the expression of two different sets of C/EBP inhibitors, CHOP and DEC2, while down-modulating C/EBPα, PPARγ and other adipogenic markers and effectors in differentiating 3T3-L1 preadipocytes and mature adipocytes. Data also suggested that the berberine-induced up-regulation of CHOP and DEC2 was attributable to selective activation of an unfolded protein response (UPR) and modified extracellular environment, respectively. As a result, the anti-adipogenic activity of berberine was diminished remarkably by adjusting the differentiation culture media and limitedly but consistently by knockdown of CHOP expression. Together, up-regulation of C/EBP inhibitors appears to underlie the berberine-induced repression of C/EBPα and PPARγ and, so, the inhibition of adipogenesis. 相似文献
38.
研究了排水条件下施用腐熟有机物料、种稻改良滨海盐渍土内源铁形态分布.结果表明,单淹水利于土中无定形氧化铁、络合态铁、有效态铁以及氧化铁活化度升高,并且络合态铁与有效态铁升高程度,低盐土壤显著;晶形氧化铁变化不明显,土中可能存在其它形态铁向晶形氧化铁转化.种稻不施有机物料,根际络合态铁和有效态铁富集;无定形氧化铁和晶形氧化铁亏缺.根际氧化铁活化度稍低于非根际.增施有机物料利于两种盐渍土根际内外铁形态向络合态铁和无定形氧化铁转化;有效态铁和氧化铁活化度提高,低盐土壤根际较明显 相似文献
39.
Heim Alexander Luster Jörg Brunner Ivano Frey Beat Frossard Emmanuel 《Plant and Soil》1999,216(1-2):103-116
In order to investigate if Al resistance in Norway spruce (Picea abies[L.] Karst.) can be attributed to similar exclusion mechanisms as they occur in several crop plants, three-year-old Norway
spruce plants were treated for one week in hydroculture with either 500 μM AlCl3 or CaCl2 solutions at pH 4. Sequential root extraction with 1 M NH4Cl and 0.01 M HCl and EDX microanalysis revealed that Al and Ca in cell walls and on the surface participated in exchange processes. About
half of the Al extracted by the sequential extraction was not exchangeable by 1 M NH4Cl. Phenolics and phosphate present in the root extracts are possible ligands for Al adsorbed to or precipitated at the root
in a non-exchangeable form. In both treatments, C release during the first period of 2 d was much higher than during the remaining
time of the experiment. Al treated plants released less total C, carbohydrates and phenolics than did Ca treated plants. Acetate
was the only organic acid anion that could be detected in some samples of both treatments. Free amino acids were present at
micromolar concentrations but as hydrolysis did not increase their yield, there was no evidence of peptide release. One to
two thirds of the released C were large enough not to pass a 1 kDa ultrafilter. The results suggest that exudation of soluble
organic complexors is not a major Al tolerance mechanism in Norway spruce, although complexation of Al by phenolic substances
released by the root could be detected by fluorescence spectroscopy. Aluminium tolerance could rather be attributed to immobilization
in the root apoplast, where strong binding sites are available or precipitation may occur.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
40.
Paramagnetic relaxation enhancements in unfolded proteins: Theory and application to drkN SH3 domain
Yi Xue Ivan S. Podkorytov D. Krishna Rao Nathan Benjamin Honglei Sun Nikolai R. Skrynnikov 《Protein science : a publication of the Protein Society》2009,18(7):1401-1424
Site‐directed spin labeling in combination with paramagnetic relaxation enhancement (PRE) measurements is one of the most promising techniques for studying unfolded proteins. Since the pioneering work of Gillespie and Shortle (J Mol Biol 1997;268:158), PRE data from unfolded proteins have been interpreted using the theory that was originally developed for rotational spin relaxation. At the same time, it can be readily recognized that the relative motion of the paramagnetic tag attached to the peptide chain and the reporter spin such as 1HN is best described as a translation. With this notion in mind, we developed a number of models for the PRE effect in unfolded proteins: (i) mutual diffusion of the two tethered spheres, (ii) mutual diffusion of the two tethered spheres subject to a harmonic potential, (iii) mutual diffusion of the two tethered spheres subject to a simulated mean‐force potential (Smoluchowski equation); (iv) explicit‐atom molecular dynamics simulation. The new models were used to predict the dependences of the PRE rates on the 1HN residue number and static magnetic field strength; the results are appreciably different from the Gillespie–Shortle model. At the same time, the Gillespie–Shortle approach is expected to be generally adequate if the goal is to reconstruct the distance distributions between 1HN spins and the paramagnetic center (provided that the characteristic correlation time is known with a reasonable accuracy). The theory has been tested by measuring the PRE rates in three spin‐labeled mutants of the drkN SH3 domain in 2M guanidinium chloride. Two modifications introduced into the measurement scheme—using a reference compound to calibrate the signals from the two samples (oxidized and reduced) and using peak volumes instead of intensities to determine the PRE rates—lead to a substantial improvement in the quality of data. The PRE data from the denatured drkN SH3 are mostly consistent with the model of moderately expanded random‐coil protein, although part of the data point toward a more compact structure (local hydrophobic cluster). At the same time, the radius of gyration reported by Choy et al. (J Mol Biol 2002;316:101) suggests that the protein is highly expanded. This seemingly contradictory evidence can be reconciled if one assumes that denatured drkN SH3 forms a conformational ensemble that is dominated by extended conformations, yet also contains compact (collapsed) species. Such behavior is apparently more complex than predicted by the model of a random‐coil protein in good solvent/poor solvent. 相似文献