全文获取类型
收费全文 | 6417篇 |
免费 | 719篇 |
国内免费 | 342篇 |
出版年
2024年 | 21篇 |
2023年 | 103篇 |
2022年 | 139篇 |
2021年 | 249篇 |
2020年 | 360篇 |
2019年 | 450篇 |
2018年 | 306篇 |
2017年 | 259篇 |
2016年 | 264篇 |
2015年 | 284篇 |
2014年 | 334篇 |
2013年 | 541篇 |
2012年 | 295篇 |
2011年 | 279篇 |
2010年 | 256篇 |
2009年 | 301篇 |
2008年 | 328篇 |
2007年 | 290篇 |
2006年 | 262篇 |
2005年 | 242篇 |
2004年 | 225篇 |
2003年 | 172篇 |
2002年 | 183篇 |
2001年 | 147篇 |
2000年 | 103篇 |
1999年 | 123篇 |
1998年 | 102篇 |
1997年 | 110篇 |
1996年 | 65篇 |
1995年 | 72篇 |
1994年 | 62篇 |
1993年 | 54篇 |
1992年 | 59篇 |
1991年 | 57篇 |
1990年 | 46篇 |
1989年 | 32篇 |
1988年 | 37篇 |
1987年 | 35篇 |
1986年 | 27篇 |
1985年 | 35篇 |
1984年 | 33篇 |
1983年 | 13篇 |
1982年 | 25篇 |
1981年 | 15篇 |
1980年 | 16篇 |
1979年 | 16篇 |
1978年 | 8篇 |
1977年 | 10篇 |
1976年 | 11篇 |
1974年 | 8篇 |
排序方式: 共有7478条查询结果,搜索用时 46 毫秒
111.
Dispersal of adult Viburnum whitefly,Aleurotrachelus jelinekii (Frauenf.) was assessed over a period of 6 years, both in the field and in the laboratory. Flight activity did not appear to be strongly affected by normal variation in either temperature or windspeed. The aerial density of flying adults decreased rapidly with distance from the host plant. Movement up to 5 m from the host plant was found to be density dependent, although there is no suggestion that longer flights become more frequent at higher population densities. More males were caught at the beginning of the season and the number of males flying increased as the population density rose. Insects were flight mature after about 3 h, but flew readily only after 2 days. Longer flights were observed from younger females at a time when they would move from old to young leaves. Landing site preference was not recorded, which contrasts with the behaviour of the cabbage whitefly. Flight in the Viburnum whitefly appears to redistribute the population within the immediate habitat, but migration did not appear to be a significant demographic factor in the isolated populations studied.
Etude de l'activité de vold d'Aleurotrachelus jelinekii peu enclin au vol
Résumé La dispersion des adultes d'Aleurotrachelus jelinekii Frauenf, tant dans la nature qu'au laboratoire, a été estimée sur une période de 6 ans. L'activité de vol n'a pas paru être fortement modifiée par les variations de la température et de la vitesse du vent. Le nombre d'adultes en vol a diminué rapidement jusqu'à 5 m de la plante hôte, et il était density-dependent, bien que rien ne prouve que les vols les plus longs deviennent plus fréquents à des densités de population plus élevées. Plus de mâles ont été capturés au début de la saison et le nombre de mâles en vol s'est accru avec la densité de la population. Les insectes étaient aptes au vol 3 heures après la mue imaginale, mais ne volaient normalement que 2 jours après. Les vols les plus longs ont été observés chez les plus jeunes femelles au moment où elles devaient migrer de feuilles âgées à des feuilles jeunes. Les lieux d'atterrissage préférés n'ont pas été décelés. Le vol chezA. jelinekii semble redistribuer la population dans l'habitat immédiat et la migration n'apparaît pas être un paramètre démographique significantif dans les populations isolées étudiées.相似文献
112.
The results of our present study indicate that 1 alpha, 25-dihydroxyvitamin D3[1 alpha, 25(OH)2D3] directly induces fusion of mouse alveolar macrophages without any participation of T-lymphocytes by a mechanism involving RNA and protein synthesis but not DNA synthesis. We have reported that 1 alpha, 25(OH)2D3 induces fusion of alveolar macrophages by a direct mechanism and by a spleen cell-mediated indirect mechanism [(1983) Proc. Natl. Acad. Sci. USA 80, 5583-5587]. Alveolar macrophages pretreated with or without anti-Thy 1.2 antibody and complement fused similarly when they were incubated with 1 alpha, 25(OH)2D3. The vitamin suppressed DNA synthesis, but it significantly enhanced RNA and protein synthesis. The 1 alpha, 25(OH)2D3-induced fusion was blocked by adding actinomycin D or cycloheximide, but not by hydroxyurea. 相似文献
113.
Summary Pieces of coverslip glass coated with various proteins were implanted under one edge of a fresh skin wound on adult newt hind limbs so that the implant served as wound bed for the migrating wound epithelium. Laminin, a protein that has been implicated as an epithelial-specific adhesin, was a moderately good migration substrate. Type-IV collagen, fibrinogen and fibronectin, however, were significantly better. Fetuin, myoglobin, and casein all proved to be very poor substrates, allowing practically no migration. The inability of fetuin, myoglobin, and casein to support migration is further evidence that the considerable migration that occurs on collagen (Donaldson et al. 1982) fibrinogen and fibronectin (Donaldson and Mahan 1983) and the moderate migration on laminin, is a relatively specific response to these proteins and is therefore of special significance. The fact that laminin is a poorer migration substrate than collagen, fibrinogen or fibronectin suggests that the absence of cell surface laminin that has been associated with epithelial movement in several studies (Stanley et al. 1981; Clark et al. 1982; Madri and Stenn 1982; Gulati et al. 1983) may promote motility by allowing epithelial cells to interact directly with other extracellular macromolecules. 相似文献
114.
Thomas P. Cogley John R. Anderson Laura J. Cogley 《International journal for parasitology》1982,12(5):473-480
Cogley T. P., Anderson J. R. and Cooley L. J. 1982. Migration of Gasterophilus intestinalis larvae (Diptera: Gasterophilidae) in the equine oral cavity. International Journal for Parasitology12: 473–480. Larvae of G. intestinalis pursued a specific migratory pathway within the equine oral cavity en route to the stomach. The larval migration included the following sequence: burrowing in the tongue mucosa, invasion of the interdental spaces, transitory attachment at the root of the tongue and movement to the stomach. The molt from first to second instar did not occur in the tongue, as commonly believed, but between the interdental spaces. Ninety five percent of the larvae invading the interdental spaces were associated with gingiva of the upper molars. SEM analysis revealed further details of the oral migration: (1) air holes excavated in the epithelium which connect with deeper burrows; (2) an intimate association between air holes and posterior spiracles of larvae; (3) precise impressions of larvae in tissue immediately surrounding the most recently formed burrows; and (4) initial larval entry into the tongue through the use of natural disruptions or healing lesions. Factors influencing the development of the oral migration are discussed. 相似文献
115.
G M Ledda A Columbano P M Rao S Rajalakshmi D S Sarma 《Biochemical and biophysical research communications》1980,95(2):816-821
In order to characterize rat liver DNA replicated on a carcinogen-damaged template, the replicated DNA was treated with S1-nuclease and the release of (14C)-dimethyl-nitrosamine induced 06-methylguanine, a lesion associated with miscoding and N-7-methylguanine, a lesion that does not miscode were monitored. The results indicated that both the methylated guanines became susceptible to S1-nuclease upon replication. However, a greater percentage of 06-methylguanine (22% of the total 06-methylguanine present in the DNA) compared to N-7-methylguanine (4% of the total N-7-methylguanine present in the DNA) was rendered acid soluble by S1-nuclease. The preferential release of 06-methylguanine compared to N-7-methylguanine from replicated DNA was interpreted to indicate its occurrence in local denatured regions probably generated as a result of misbase pairing. 相似文献
116.
Perry Karfunkel 《Development genes and evolution》1977,181(1):31-40
Summary At the end of gastrulation, the lateral mesoderm of amphibian embryos migrates ventrally between the ectoderm and the endoderm. The present study is an examination of the morphology of the leading cells of the mesodermal sheet and of the substratum over which they move (the inner surface of the ectoderm). The cells of the leading edge of the mesoderm are generally round, with very short and narrow flattened projections in the forward direction. These projections do not have a ruffled morphology, regardless of whether fixation is carried out before or after the ectoderm and mesoderm are dissected away from the endoderm. The inner surface of the ectoderm is covered with fine (450–500A) filamentous extracellular material and the ectoderm cells sometimes extend cytoplasmic processes (approx. 0.1 wide) onto the leading surface of the mesoderm or onto adjacent ectoderm cells. These studies indicate that the morphology of cell migration in amphibians is closer to that seen inFundulus than to that characteristic of chick or mammalian cells.This paper is dedicated to the memory of Mac V. Edds, Jr., who warmly encouraged the developmental biologists of the Pioneer Valley 相似文献
117.
Summary Cells of embryos carrying a lethal nucleolar mutation have been maintained in vitro for extended periods of time. Normally
these mutants live only 9 to 12 days after fertilization but their cells in culture will survive for more than 3 months. The
extent of ribosomal RNA (rRNA) synthesis was determined in primary cultures prepared from normal embryos and nucleolar mutants
having different numbers of ribosomal RNA genes. We found that the accumulation of radioactivity into rRNA for normal and
mutant embryos was similar in vivo and in vitro. In primary cultures of normal embryos which have two nucleoli per cell and
mutant embryos which have only one nucleolus per cell, the incorporation of radio-activity into rRNA was similar even though
the normal cells have twice as many rRNA genes. Thus the mechanism which regulates dosage compensation of the rRNA genes operates
both in vivo and in vitro.
This work was supported by Grant GB38651 from the National Science Foundation. 相似文献
118.
Evidence of a genetic component in the seasonal return pattern of Atlantic salmon, Salmo salar L. 总被引:1,自引:0,他引:1
We tested the null hypothesis that differences in the seasonal return patterns between stocks of Atlantic salmon, Salmo salar L., are a result of a direct response to the environment, and not under genetic control. Two stocks were used in the experiments, originating from the R. Figga and R. Imsa, respectively. In their native habitat fish from the former are known to return to the home stream as adult salmon early in the summer, while from the latter return during late summer and autumn. By rearing these stocks in the same hatchery and releasing smolts of both stocks together at three sites in southern Norway, it was demonstrated that salmon from the R. Figga stock returned earlier to coastal Norway than salmon from the R. Imsa stock, as maturing adults. Thus, we reject the hypothesis that these stocks are genetically identical in this trait. Within both stocks, multi-sea-winter fish returned earlier than one-sea-winter fish. Within stocks, there was no significant difference in time of return between salmon released as 1- and 2-year-old smolts, or between fish reared from parents ascending the R. Imsa early or late in the season. 相似文献
119.
Behavioural thermoregulation and homing by spring chinook salmon, Oncorhynchus tshawytscha (Walbaum), in the Yakima River 总被引:1,自引:1,他引:0
Temperature-sensitive radio transmitters were employed to study the patterns of behavioural thermoregulation, habitat preference and movement of 19 adult spring chinook salmon, Oncurhynchus tshawytscha (Walbaum), in the Yakima River. During the 4 months prior to spawning, fish maintained an average internal temperature 2.5°C below ambient river temperature. This represented a 12 to 20% decrease in basal metabolic demand or a saving of 17.3 to 29.9 calkg−1 h−1 . Fish were most commonly associated with islands, pools, and rock out-croppings along stream banks. Homing behaviour appeared to be modified to optimize temperature regimes and energy conservation. As the time of spawning approached, fish left thermal refuges and migrated to spawning grounds upstream and downstream of refuge areas. Although spring chinook salmon residing within cool-water refuges may be capable of mitigating sub-lethal temperature effects, cool-water areas need to be abundant and available to the fish. The availability of suitable thermal refuges and appropriate holding habitat within mainstem rivers may affect long-term population survival. 相似文献
120.
Louise R. Romanow Orlando M. B. Ponti Chris Mollema 《Entomologia Experimentalis et Applicata》1991,60(3):247-259
Life history parameters including longevity, developmental time, and reproduction were determined for whiteflies, Trialeurodes vaporariorum (Westwood), kept in clip-on cages on susceptible parent, Lycopersicon esculentum, resistant parent, L. hirsutum glabratum, and intermediate breeding lines of tomato. Using the Lewontin triangular reproductive function, the intrinsic rate of increase (rm) was calculated for each genotype. This is an elegant tool for detecting resistance, as it incorporates all salient factors of life history. Comparing rm to the other parameters measured, selection criteria were chosen. Developmental time is not a suitable selection criterion as it varies very little even between the most susceptible and the most resistant genotypes. Additionally, developmental time of T. vaporariorum offspring was found to be highly significantly correlated to parental age on all tomato genotypes except L. hirsutum glabratum. Total reproduction, truncated population counts, and longevity appear good criteria for selection. This test, focusing on antibiotic factors, shows large differences between the resistant and susceptible parent genotypes, but much smaller differences between the breeding lines and the susceptible parent. Earlier screenings relying on a variety of tests appear to have maintained antixenotic rather than antibiotic properties in the breeding lines. 相似文献