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121.
An incubation protocol to immunolabel Lowicryl semithin sections was applied to paraffin probes. To improve the labeling density, colloidal gold complexes of different preparations and sizes were compared. The type of colloidal gold preparation used was found to affect the specificity of the immunostaining. Gold colloid of 5 nm diameter particle size prepared with white phosphorus minimized nonspecific background labeling of β-casein in paraffin embedded sections of the mammary epithelium of pregnant mice. Gold colloids of 5 nm and 9 nm diameter particle size prepared in varying concentrations of tannic acid generated significant nonspecific staining in similar tissue preparations. 相似文献
122.
《Biotechnic & histochemistry》2013,88(5):289-292
Yeast mlcrocolonies grown on 5 × 10 nun rectangles of membrane (Millipore) filter are treated through the membrane pores with 0.5% sodium azide for 2-3 hr, 10% perchloric acid for 4 days, and Giemsa stain for 1 day. After dehydration in air, they are permanently mounted. Similar preparations can be made with cells from sporularion cultures. 相似文献
123.
Shunro Kohbata 《Microbiology and immunology》1996,40(10):711-716
Nocardia asteroides GUH-2 was not acid-fast when grown in brain heart infusion (BHI) broth. When grown in BHI broth supplemented with paraffin, many filamentous cells showed acid-fastness after treatment with 1% acid-alcohol as the decolorizing agent. When treated with 3% acid-alcohol, filamentous cells were not acid-fast. In addition to the acid-fast filamentous cells of nocardiae, unknown acid-fast spherical bodies were observed in the paraffin-supplemented BHI broth cultures. 相似文献
124.
Paraffin wax embedding methods suitable for demonstrating the distribution of enzyme activity in tissues sections are uncommon; most procedures rely on the use of frozen section techniques. This paper describes a system for demonstrating certain enzymes which involves incubation of the tissue with appropriate substrates before a Paramat wax embedding procedure. While it has distinct merits of its own, the procedure is eminently suitable for use where a cryostat is not available; it can also be readily applied to other enzymes and tissues. 相似文献
125.
We describe chemical dehydration with 2,2-dimethoxypropane (DMP) for rapid paraffin embedding using a mixture of DMP and mineral oil followed by mineral oil as clearing intermediates. This method is useful for classical histological techniques as well as for histochemistry and immunocytochemistry. 相似文献
126.
Stephan Brandt Christina Walz Martina Schad Nada Pavlovic Julia Kehr 《Plant Molecular Biology Reporter》2003,21(4):417-427
Many physiological processes are limited to specific tissues or even specific cell types. Analysing entire plants or organs
results in averaged data of all cell types contained in the sample; thus, specific metabolic functions cannot be assigned
to individual cell types. A higher spatial resolution is required. By microdissecting plant organs, homogeneous material can
be obtained. If a suitable amount of material is collected, standard analytical methods can be applied to elucidate cell type-specific
processes. The collection of sufficient quantities of homogeneous material can be done by means of mechanical microdissection.
This technique is a low-cost alternative to laser-coupled microdissection techniques. Here we describe a protocol for chisel-assisted
mechanical microdissection of embedded plant material and demonstrate that the collected material is suitable to obtain nucleic
acids and proteins. 相似文献