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81.
Alan H. Teramura Mark C. Perry John Lydon Marla S. McIntosh Edwin G. Summers 《Physiologia plantarum》1984,60(4):484-492
Soybean { Glycine max (L.) Merr. ev. Essex} was grown from seed in a greenhouse under ultraviolet-B (UV-B, 280–320 nm) radiation supplied by filtered FS-40 sunlamps. On a weighted, total daily dose basis these plants received either 0 (control) or 2875 effective J m−2 day−1 UV-BBE . When weighted with the generalized plant action spectrum (Caldwell 1971), this simulated the solar ultraviolet-B irradiance expected to occur at College Park, Maryland, USA (39°N) in the event the global stratospheric ozone column is reduced by 23%. The effects of ultraviolet radiation on the photosynthetic recovery from water stress were measured with an infrared gas analyzer. These effects were examined in plants which were either well-watered or previously preconditioned to water stress, during two distinct phenological stages of development. During the early stages of soybean growth, enhanced levels of UV-B reduced net photosynthesis by 25%, and water stress also reduced photosynthesis to nearly the same extent (by 20%). The combination of these two stresses resulted in smaller biomass than that produced by plants exposed to either stress independently. Photosynthesis in older, larger plants was much more sensitive to water stress and was reduced by as much as 50–60% in non-preconditioned plants. Although non-irradiated, non-preconditioned (control) plants recovered to only within 60% of their prestressed value, preconditioned plants recovered to within 70–80% during the 3 day recovery period. Both water stress and UV-B radiation affected non-stomatal conductance, while stomatal conductance was primarily affected by water stress. 相似文献
82.
The possibility that changes in the plasticity of expanding cell walls are involved in regulating early leaf growth responses to nutrient deficiencies in monocot plants was investigated. Intact maize seedlings (Zea mays L.) which were hydroponically grown with their roots in low-nutrient solution (1 mol m?3 CaCl2) showed early inhibition of first-leaf growth, as compared with seedlings on complete nutrient solution. This early inhibition of leaf growth was not associated with reduced cell production. However, segmental elongation along the cell expansion zone at the base of the leaf and the lengths of mature epidermal cells were reduced by the low-nutrient treatment. Solute (osmotic) potentials in the expanding leaf tissues were unchanged. In contrast, low-nutrient treatments significantly altered leaf plasticity, i.e. the irreversible extension caused by applying a small force in the direction of leaf growth. For example, in vivo plasticity decreased, along with leaf growth, after transfer of seedlings from complete nutrient solution to low-nutrient solution for 15 h. Conversely, in vivo plasticity increased, along with leaf growth, after transfer of plants previously grown on low-nutrient solution to complete nutrient solution for 15 h. The nutrient treatments also induced similar changes in the in vitro plasticity of the expanding leaf cell walls. There were no consistent changes in elasticity. Thus, reductions in the plasticity of expanding leaf cell walls appear to be involved in controlling the early inhibition of maize leaf growth by root imposition of nutrient stress. 相似文献
83.
The potassium salt-induced transient increase of delayed fluorescence yield was studied in pea chloroplasts treated with 3-(3,4-dichlorophenyl)-1,1-dimethylurea.A simple kinetic model is proposed to account for the actinic light intensity dependence of the delayed fluorescence enhancement by the transmembrane diffusion potential induced by sudden salt addition. The electric field dependence of the rate constants for the recombination of primary separated charges with and without subsequent electronic excitation of reaction center chlorophyll was obtained.From the value of enhancement of delayed fluorescence by salt concentration gradients at saturating actinic light intensity, it is concluded that the distance, normal to thylakoid membrane surface, between the primary acceptor and the donor of Photosystem II is smaller than the membrane thickness. 相似文献
84.
A global study of relationships between leaf traits, climate and soil measures of nutrient fertility 总被引:7,自引:0,他引:7
Jenny C. Ordoñez Peter M. van Bodegom Jan-Philip M. Witte Ian J. Wright Peter B. Reich Rien Aerts 《Global Ecology and Biogeography》2009,18(2):137-149
Aim This first global quantification of the relationship between leaf traits and soil nutrient fertility reflects the trade‐off between growth and nutrient conservation. The power of soils versus climate in predicting leaf trait values is assessed in bivariate and multivariate analyses and is compared with the distribution of growth forms (as a discrete classification of vegetation) across gradients of soil fertility and climate. Location All continents except for Antarctica. Methods Data on specific leaf area (SLA), leaf N concentration (LNC), leaf P concentration (LPC) and leaf N:P were collected for 474 species distributed across 99 sites (809 records), together with abiotic information from each study site. Individual and combined effects of soils and climate on leaf traits were quantified using maximum likelihood methods. Differences in occurrence of growth form across soil fertility and climate were determined by one‐way ANOVA. Results There was a consistent increase in SLA, LNC and LPC with increasing soil fertility. SLA was related to proxies of N supply, LNC to both soil total N and P and LPC was only related to proxies of P supply. Soil nutrient measures explained more variance in leaf traits among sites than climate in bivariate analysis. Multivariate analysis showed that climate interacted with soil nutrients for SLA and area‐based LNC. Mass‐based LNC and LPC were determined mostly by soil fertility, but soil P was highly correlated to precipitation. Relationships of leaf traits to soil nutrients were stronger than those of growth form versus soil nutrients. In contrast, climate determined distribution of growth form more strongly than it did leaf traits. Main conclusions We provide the first global quantification of the trade‐off between traits associated with growth and resource conservation ‘strategies’ in relation to soil fertility. Precipitation but not temperature affected this trade‐off. Continuous leaf traits might be better predictors of plant responses to nutrient supply than growth form, but growth forms reflect important aspects of plant species distribution with climate. 相似文献
85.
86.
Pyriforms are ovarian follicle nurse cells that undergo apoptosis at the end of previtellogenesis and are completely eliminated
by the epithelium. This event is accompanied by the active transfer of organelles and macromolecules to the oocyte via an
intercellular bridge. Since it would be a nonsense for damaged mitochondria to reach the oocyte, we have postulated that pyriform
cells have adapted their apoptotic machinery to prevent mitochondrial degradation. To verify this hypothesis, we have studied
mitochondrial morphology and functionality during follicle cell regression. Cytological and biochemical evidence indicates
that mitochondria in pyriforms maintain their size, organization and membrane potential. This clearly indicates that they
are not involved in apoptosis signalling/progression. This block would favour both the oocyte, by increasing the pool of organelles
available from follicle cells, and also the regressing pyriforms, by maintaining the energy resources required for completion
of their nurse function. The block is probably attributable to an over-expression of Bcl-2 and might be carried out by sequestering
cytochrome c inside the organelles. As demonstrated by in vitro experiments, the mitochondrial apoptosis pathway can be activated
by stress induction, such as serum deprivation, but not following physiological pro-apoptotic signalling, such as treatment
with gonadotrophin-releasing hormone.
These studies were supported by a grant from the MIUR (PRIN project: Molecular responses of embryonic, differentiated and
tumoral cells exposed to cadmium intoxication). 相似文献
87.
Leena Peltonen Piritta Koistinen Milja Karjalainen Antti Häkkinen Jouni Hirvonen 《AAPS PharmSciTech》2002,3(4):52-58
The aim of this study was to formulate nanoparticles from poly(I)lactide by a modified nanoprecipitation method. The main
focus was to study the effect of cosolvent selection on the shape, size, formation efficiency, degree of crystallinity, x-ray
diffraction (XRD) reflection pattern, and zeta potential value of the particles. Low-molecular-weight (2000 g/mol) poly(I)lactide
was used as a polymer, and sodium cromoglycate was used as a drug. Acetone, ethanol, and methanol were selected as cosolvents.
Optimal nanoparticles were achieved with ethanol as a cosolvent, and the formation efficiency of the particles was also higher
with ethanol as compared with acetone or methanol. The particles formulated by ethanol and acetone appeared round and smooth,
while with methanol they were slightly angular. When the volume of the inner phase was decreased during the nanoprecipitation
process, the mean particle size was also decreased with all the solvents, but the particles were more prone to aggregate.
The XRD reflection pattern and the degree of crystallinity were more dependent were more prone to aggregate. The XRD reflection
pattern and the degree of crystallinity were more dependent on the amount of the solvents in the inner phase than on the properties
of the individual cosolvents. The zeta potential values of all the particle batches were slightly negative, which partially
explains the increased tendency toward particle aggregation. 相似文献
88.
纤维连接蛋白对支气管上皮细胞抗氧化损伤保护作用的研究 总被引:4,自引:0,他引:4
目的和方法:为验证整合素分子激活对支气管上皮细胞(BEC)的抗氧化性保护作用,本实验用臭氧(O3)攻击培养的兔BEC,测定细胞的^3H释放率、乳酸脱氢酶(LDH)释放活性及脂质过氧化产物丙二醛(MDA)含量,反映细胞损伤程度;观察纤维连接蛋白(Fn)及人工合成的精-甘-天冬氨酸片段(RGD肽)的保护效应。结果:①臭氧攻击使BEC的^3H释放率增高,Fn处理可减少臭氧所致的^3H释放,钙调素抑制剂W7能抑制Fn的这一作用,RGD可减轻臭氧所致的^3H释放;②臭氧攻击后细胞上清液中LDH释放增多,Fn或RGD处理均能降低LDH释放,W7阻断Fn的这一效奕;③臭氧作用后明显提高细胞内MDA含量,Fn或RGD可降低MDA含量;④臭氧攻击使细胞内GSH含量下降,Fn或RGD可增加BEC内GSH的含量;⑤Fn可增强BEC内过氧化氢酶(CAT)活性,但可被W7阻断,RGD则显示有剂量依赖性促进作用。结论:Fn及其特异识别片段与BEC的整合素分子结合后,可减轻臭氧对BEC细胞的损伤,其机理与经钙调素途径上调BEC抗氧化能力有关。 相似文献
89.
The characteristics of Cl– transport in isolated tonoplast vesicles from red-beet (Beta vulgaris L.) storage tissue have been investigated using the Cl–-sensitive fluorescent probe, 6-methoxy-1-(3-sulfonatopropyl)-quinolinium (SPQ). The imposition of (inside) positive diffusion potentials, generated with K+ and valinomycin, increased the initial rate of Cl– transport, demonstrating that Cl– could be electrically driven into the vesicles. Chloride influx was unaffected by SO
4
2-
, but was competitively blocked by NO
3
–
, indicating that both Cl– and NO
3
–
may be transported by the same porter. In some preparations, increases in free-Ca2+ concentration from 10–8 to 10–5 mol·dm–3 caused a significant decrease in Cl– influx, which may indicate that cytosolic Ca2+ concentration has a role in controlling Cl– fluxes at the tonoplast. However, this effect was only seen in about 50% of membrane preparations and some doubt remains over its physiological significance. A range of compounds known to block anion transport in other systems was tested, and some partially blocked Cl– transport. However, many of these inhibitors interfered with SPQ fluorescence and so only irreversible effects could be tested. The results are discussed in the context of recent advances made using the patch-clamp technique on isolated vacuoles.Abbreviations and Symbols BTP
1,3-bis[tris(hydroxymethyl)-methylamino]propane
- DTT
dithiothreitol
- EDTA
ethylenediaminetetraacetic acid
-
membrane potential
- pH
pH gradient
- SPQ
6-methoxy-1-(3-sulfonatopropyl)quinolinium
- Tricine
N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl] glycine 相似文献
90.
Canopy height variation and environmental heterogeneity in the tropical dry forests of coastal Oaxaca,Mexico 下载免费PDF全文
Despite its importance for carbon storage and other ecosystem functions, the variation in vegetation canopy height is not yet well understood. We examined the relationship between this community attribute and environmental heterogeneity in a tropical dry forest of southern Mexico. We sampled vegetation in 15 sites along a 100‐km coastal stretch of Oaxaca State, and measured the heights of all woody plants (excluding lianas). The majority of the ca. 4000 individuals recorded concentrated in the 4–8 m height range. We defined three plant sets to describe overall community canopy height at each site: a set including all plants, a set made up by the tallest plants representing 10 percent of all individuals, and a set comprising the 10 tallest plants. For each site we computed maximum height and the mean and median heights of the three sets. Significant collinearity was observed between the seven resulting height variables, but null distributions constructed through bootstrap revealed their different behaviors as functions of species richness and density of individuals. Through linear modeling and a model selection procedure, we identified 21 models that best described the variation in canopy height variables. These models pointed out to soil (measured as PC1 of a principal component analysis performed on 10 soil variables), water stress, and elevation as the main drivers of canopy height variation in the region. In the event of increasing water stress resulting from global climate change, the studied tropical dry forests could become shorter and thus decrease their carbon storage potential. 相似文献