全文获取类型
收费全文 | 1736篇 |
免费 | 211篇 |
国内免费 | 49篇 |
出版年
2024年 | 9篇 |
2023年 | 54篇 |
2022年 | 47篇 |
2021年 | 70篇 |
2020年 | 91篇 |
2019年 | 86篇 |
2018年 | 81篇 |
2017年 | 73篇 |
2016年 | 76篇 |
2015年 | 66篇 |
2014年 | 71篇 |
2013年 | 127篇 |
2012年 | 112篇 |
2011年 | 61篇 |
2010年 | 50篇 |
2009年 | 67篇 |
2008年 | 78篇 |
2007年 | 84篇 |
2006年 | 62篇 |
2005年 | 56篇 |
2004年 | 66篇 |
2003年 | 56篇 |
2002年 | 50篇 |
2001年 | 40篇 |
2000年 | 23篇 |
1999年 | 30篇 |
1998年 | 15篇 |
1997年 | 32篇 |
1996年 | 23篇 |
1995年 | 28篇 |
1994年 | 26篇 |
1993年 | 24篇 |
1992年 | 30篇 |
1991年 | 23篇 |
1990年 | 14篇 |
1989年 | 21篇 |
1988年 | 10篇 |
1987年 | 4篇 |
1986年 | 8篇 |
1985年 | 10篇 |
1984年 | 7篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1981年 | 3篇 |
1979年 | 4篇 |
1977年 | 2篇 |
1976年 | 7篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 2篇 |
排序方式: 共有1996条查询结果,搜索用时 390 毫秒
911.
912.
913.
L.I.I. Ouoba C. Kando C. Parkouda H. Sawadogo‐Lingani B. Diawara J.P. Sutherland 《Journal of applied microbiology》2012,113(6):1428-1441
Aim
To investigate physicochemical characteristics and especially genotypic diversity of the main culturable micro‐organisms involved in fermentation of sap from Borassus akeassii, a newly identified palm tree from West Africa.Methods and Results
Physicochemical characterization was performed using conventional methods. Identification of micro‐organisms included phenotyping and sequencing of: 26S rRNA gene for yeasts, 16S rRNA and gyrB genes for lactic acid bacteria (LAB) and acetic acid bacteria (AAB). Interspecies and intraspecies genotypic diversities of the micro‐organisms were screened respectively by amplification of the ITS1‐5.8S rDNA‐ITS2/16S‐23S rDNA ITS regions and repetitive sequence‐based PCR (rep‐PCR). The physicochemical characteristics of samples were: pH: 3·48–4·12, titratable acidity: 1·67–3·50 mg KOH g?1, acetic acid: 0·16–0·37%, alcohol content: 0·30–2·73%, sugars (degrees Brix): 2·70–8·50. Yeast included mainly Saccharomyces cerevisiae and species of the genera Arthroascus, Issatchenkia, Candida, Trichosporon, Hanseniaspora, Kodamaea, Schizosaccharomyces, Trigonopsis and Galactomyces. Lactobacillus plantarum was the predominant LAB species. Three other species of Lactobacillus were also identified as well as isolates of Leuconostoc mesenteroides, Fructobacillus durionis and Streptococcus mitis. Acetic acid bacteria included nine species of the genus Acetobacter with Acetobacter indonesiensis as predominant species. In addition, isolates of Gluconobacter oxydans and Gluconacetobacter saccharivorans were also identified. Intraspecies diversity was observed for some species of micro‐organisms including four genotypes for Acet. indonesiensis, three for Candida tropicalis and Lactobacillus fermentum and two each for S. cerevisiae, Trichosporon asahii, Candida pararugosa and Acetobacter tropicalis.Conclusion
fermentation of palm sap from B. akeassii involved multi‐yeast‐LAB‐AAB cultures at genus, species and intraspecies level.Significance and Impact of the Study
First study describing microbiological and physicochemical characteristics of palm wine from B. akeassii. Genotypic diversity of palm wine LAB and AAB not reported before is demonstrated and this constitutes valuable information for better understanding of the fermentation which can be used to improve the product quality and develop added value by‐products. 相似文献914.
Seung Won Nam Woongghi Shin D. Wayne Coats Jong Woo Park Wonho Yih 《The Journal of eukaryotic microbiology》2012,59(6):625-636
Mesodinium rubrum Lohmann is a photosynthetic marine ciliate that has functional chloroplasts of cryptophyte origin. Little is known about the oral ultrastructure of M. rubrum compared with several reports on the sequestration of nuclei and plastids from prey organisms, such as Geminigera cryophila and Teleaulax species. Here, we describe the fine structure of the oral apparatus of a M. rubrum strain from Gomso Bay, Korea. The cytopharynx was cone‐shaped and supported by 20–22 ribbons of triplet microtubules. At the anterior end of the cytopharynx, an annulus anchored small cylinders composed of 11 microtubules. The small cylinders were spaced at regular intervals, each reinforced by one set of the triplet microtubules. At the opening of the cytostome, larger 14‐membered microtubular cylinders were set adjacent to the small, 11‐membered microtubular cylinders, each pair surrounded by separate membranes, however, only the large cylinders extended into the oral tentacles. There were 20–22 oral tentacles each having one to five extrusomes at its tip. At the anterior end of the oral apparatus, microtubular bands supporting the cytostome curved posteriad, extending beneath the cell cortex to the kinetosomes of the somatic cirri. The microtubular bands were connected by striated fibers and originated from kinetosomes anchored by fibers. Each cirrus consisted of eight cilia associated with 16 kinetosomes. The ultrastructure of M. rubrum from Korea provides information useful for taxonomic characterization of the genus Mesodinium and relevant to developing a better understanding of the acquisition of foreign organelles through phagocytosis by M. rubrum. 相似文献
915.
916.
917.
Azadirachtin: structural requirements for reducing growth and increasing mortality in lepidopterous larvae 总被引:3,自引:0,他引:3
M. S. J. Simmonds W. M. Blaney S. V. Ley J. C. Anderson P. L. Toogood 《Entomologia Experimentalis et Applicata》1990,55(2):169-181
Spodoptera littoralis, Spodoptera frugiperda and Heliothis virescens were exposed to azadirachtin and 23 other related compounds in three bioassays: oral cannulation, haemolymph injection and topical application. Spodoptera littoralis was susceptible to a larger proportion of the compounds than either of the other species. Overall, azadirachtin, 22,23-dihydroazadirachtin and 1-detigloyl-22,23-dihydroazadirachtin were the most active compounds. The nature of the substitutes at C-1 and C-3 of the decalin ring affects the potency of the compounds, as does the addition of substitutes to C-22,23. Generally, larvae were less sensitive to compounds when they were topically applied than when they were cannulated into the gut or injected into the haemolymph.
Résumé Spodoptera littoralis, S. frugiperda et Heliothis virescens ont été exposés à l'azadirachtine et 23 autres substances voisines dans 3 expériences: canulation orale, injection d'haemolymphe et application topique. S. littoralis est sensible à un plus grand nombre de substances que les 2 autres espèces. De toutes façons, les substances les plus actives ont été l'azadirachtine, la 22,23-dihydroazadirachtine et la 1-détigloyl-22,23-dihydroazadirachtine. La nature des substitutions en C-1 et C-3 de l'anneau décaline modifie la puissance des substances, tout comme les substitutions en C-22,23. D'une façon générale, les chenilles ont été moins sensibles aux applications topiques qu'aux canulations dans le tube digestif ou aux injections dans l'haemolymphe.相似文献
918.
J Riley 《International journal for parasitology》1973,3(2):146-152
The buccal complex of the haematophagous pentastomid Reighardia sternae has been described. It consists of a buccal capsule containing a prominent oral papilla communicating with a heavily chitinized pharynx which is crescentic in cross section. The chitinous skeleton of the buccal apparatus provides a framework for the attachment of various muscle systems, the contractions of which have been related to the mode of operation of the oral papilla and pharnyx during the feeding process. Particular emphasis was placed on the possible function of the oral papilla in the initial rupture of a blood capillary, and on the pumping action of the pharynx during the ingestion of the liberated blood. 相似文献
919.
920.